BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0341.0034.p (398 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value A6LZ65.1 A6LZ65_CLOB8 Citrate transporter 416 e-114 C1I537.1 C1I537_9CLOT Citrate transporter 231 1e-58 B0KC98.1 B0KC98_THEP3 Citrate transporter 223 3e-56 B0K357.1 B0K357_THEPX Citrate transporter 223 3e-56 B7R8E1.1 B7R8E1_9THEO TRAP transporter, DctM-like membrane protein 218 1e-54 >A6LZ65.1 A6LZ65_CLOB8 Citrate transporter Length = 430 Score = 416 bits (1068), Expect = e-114, Method: Compositional matrix adjust. Identities = 212/367 (57%), Positives = 256/367 (69%) Query: 16 SDNPDTFTITKGVLAGGAMKLSTAIAGLIFGAWFGQILNKVGITKTIIRKAAELAGDKPX 75 S +P FTIT VL G+M++S+AIAGLIFGAWFGQILNKVGITKTIIRKAAELAGDKP Sbjct: 47 SSDPKEFTITANVLEAGSMRMSSAIAGLIFGAWFGQILNKVGITKTIIRKAAELAGDKPV 106 Query: 76 XXXXXXXXXXXXXXXXXNGLGMVILVGTIAIPIMLTAGLKPFXXXXXXXXXXXXXXXXXX 135 +GLGMVILVGTI IPIMLTAGL P Sbjct: 107 IIALTFLIAASIIFSAASGLGMVILVGTIIIPIMLTAGLSPLVSALVLLFANAIGVTFYV 166 Query: 136 STWAIYTDVLKVPTDTIASYSLICAVPLIVIALIMIVYYTRKDGKVRRAWAMPLKQEFQN 195 WAIY DVLK+ T+ IA+YSLI AVPLI++A++ I+YY K K+++AW+MPL E Q Sbjct: 167 GGWAIYKDVLKLTTEQIANYSLIPAVPLIIVAVVTILYYNSKGAKLKKAWSMPLNNEGQL 226 Query: 196 QDTKNVRSIAXXXXXXXXXXXXXXKVDIVSAVFMGALVTLLLATPKRPIHVLSSALVEGI 255 + NVRSIA K+ IV +V +G ++TL+LATPKRP+HVLSSAL+EGI Sbjct: 227 GNNMNVRSIALISPIIPVIFAFTLKLSIVPSVVLGIVITLILATPKRPVHVLSSALIEGI 286 Query: 256 QDVXXXXXXXXXXXXXXSAVTAPQVASLIQPLIEGMIPTSPLMYILVFTLLSPLAIYRGP 315 QD +AV A VA++++P+IE +IP SPL YILVFTLLSPLAIYRGP Sbjct: 287 QDTAGALALLIGIGMVLNAVMAEPVAAILKPMIETIIPHSPLTYILVFTLLSPLAIYRGP 346 Query: 316 LNVWGLGSGIAALIVAGGMAPVAAMLALRIVSNLQAVSDPTNSHNVWISDYTKTDIIETL 375 N++GLGSGIAAL+VA GM PVAAM+ALR+ SNLQAV DPTNSHNVW+SDYTKTD+ E L Sbjct: 347 FNIYGLGSGIAALLVAAGMNPVAAMVALRVNSNLQAVCDPTNSHNVWVSDYTKTDVNEIL 406 Query: 376 RKTLPWM 382 +KT+ W+ Sbjct: 407 KKTIIWL 413 >C1I537.1 C1I537_9CLOT Citrate transporter Length = 435 Score = 231 bits (589), Expect = 1e-58, Method: Compositional matrix adjust. Identities = 133/377 (35%), Positives = 201/377 (53%), Gaps = 3/377 (0%) Query: 23 TITKGVLAGGAMKLSTAIAGLIFGAWFGQILNKVGITKTIIRKAAELAGDKPXXXXXXXX 82 T+ V+ G+ +L+ + IFGA F +++ K GI+ TIIRKAAELAGDKP Sbjct: 61 TLMAHVIGAGSSRLAGTMMAAIFGAMFAKVIQKQGISNTIIRKAAELAGDKPLAIAFILT 120 Query: 83 XXXXXXXXXXNGLGMVILVGTIAIPIMLTAGLKPFXXXXXXXXXXXXXXXXXXSTWAIYT 142 G G VI+V TI IP+ML+AG+KP + + Y Sbjct: 121 TACAIVFSALGGAGAVIMVATIVIPLMLSAGIKPKVAAGSFLMGLGLGGLFNVANYQFYV 180 Query: 143 DVLKVPTDTIASYSLICAVPLIVIALIMIVYYTRKDGKVRRAWAMPLKQEFQNQDTKNVR 202 DV+ + + + S SL+ + IV+ I+ R VR WAMP F+ + +V Sbjct: 181 DVIGMDMELVKSTSLVLGLISIVVTAAYILINVRSK-SVRSTWAMP-NSNFETKTHDDVN 238 Query: 203 SIAXXXXXXXXXXXXXXKVDIVSAVFMGALVTLLLATPKRPIHVLSSALVEGIQDVXXXX 262 A K ++ + + T+L+ P + I + +S+LVEGIQDV Sbjct: 239 VFALISPLLPILLVFFFKFSAELSLLISVVYTILVTKPSQIIQIATSSLVEGIQDVAGVI 298 Query: 263 XXXXXXXXXXSAVTAPQVASLIQPLIEGMIPTSPLMYILVFTLLSPLAIYRGPLNVWGLG 322 + V+A +L+QPLI ++PTSP+ YI++FT+ SPLA+YRGPLN++GLG Sbjct: 299 GLMIGIGILLNGVSANATIALMQPLIGAILPTSPIPYIIIFTIASPLALYRGPLNLYGLG 358 Query: 323 SGIAALIVAGG-MAPVAAMLALRIVSNLQAVSDPTNSHNVWISDYTKTDIIETLRKTLPW 381 SGI ++VA G ++P A +ALR +Q +SDPTN+HNV ++D+ K D+ L+ TLP+ Sbjct: 359 SGIGNVMVAAGTLSPAAIGMALRATGVVQGISDPTNTHNVIVADFAKVDVNSVLKSTLPY 418 Query: 382 MFVAVLISMVIAGLVAF 398 + +S++ LV F Sbjct: 419 TVIITCLSLIYTALVIF 435 >B0KC98.1 B0KC98_THEP3 Citrate transporter Length = 431 Score = 223 bits (568), Expect = 3e-56, Method: Compositional matrix adjust. Identities = 131/384 (34%), Positives = 204/384 (53%), Gaps = 15/384 (3%) Query: 24 ITKGVLAGGAMKLSTAIAGLIFGAWFGQILNKVGITKTIIRKAAELAGDKPXXXXXXXXX 83 I ++ GA++L +A+A IFGA QI+NK GI+++I+R AAEL GD+P Sbjct: 48 ILSNIIGAGAVRLQSAMAAAIFGAILAQIINKTGISESIVRTAAELGGDRPIIVALAITF 107 Query: 84 XXXXXXXXXNGLGMVILVGTIAIPIMLTAGLKPFXXXXXXXXXXXXXXXXXXSTWAIYTD 143 GLG VI+VGTI +PI+++ G++P +A Y Sbjct: 108 AVALIFTSLGGLGSVIMVGTIVLPILISVGIEPVIAASLLLMGLNLGGLFNVMNYAFYQS 167 Query: 144 VLKVPTDTIASYSLICAVPLIVIALIMIVYYTRKDGKVRRAWAMPLKQEFQNQDTKN--- 200 L + TI S++L + + + L++ ++ K AWAMP E Q + + Sbjct: 168 ALNLDMATIKSFALAFGI-IAAVVLLLFIFINVPKKKTVTAWAMP--TETQEEKPRASWY 224 Query: 201 --------VRSIAXXXXXXXXXXXXXXKVDIVSAVFMGALVTLLLATPKRPIHVLSSALV 252 V + ++DI+SA+ +G L +L P+ ++L+S+++ Sbjct: 225 ALLTPIIPVLLLFIWPLIFKDAEGKPIQIDIISAMLIGILYGILTTRPREIKNLLTSSII 284 Query: 253 EGIQDVXXXXXXXXXXXXXXSAVTAPQVASLIQPLIEGMIPTSPLMYILVFTLLSPLAIY 312 EGI+DV AV A+LI P+I ++PTS + Y+L F +LSPLA+Y Sbjct: 285 EGIKDVAPALGLMIGIGMLLLAVMDKTTAALISPIISKILPTSKIGYVLFFVILSPLALY 344 Query: 313 RGPLNVWGLGSGIAALIVAGGMAPVAAML-ALRIVSNLQAVSDPTNSHNVWISDYTKTDI 371 RGPLN+WGLGSG+AAL+++ G+ P A++ AL N+Q V DPTN+HNVW + +T TD Sbjct: 345 RGPLNLWGLGSGVAALMISAGILPPQAIMGALIATGNIQGVCDPTNTHNVWTAYFTNTDT 404 Query: 372 IETLRKTLPWMFVAVLISMVIAGL 395 L+KTLP+M + VL+ ++ L Sbjct: 405 TTILKKTLPYMLIVVLLDIIYVAL 428 >B0K357.1 B0K357_THEPX Citrate transporter Length = 431 Score = 223 bits (568), Expect = 3e-56, Method: Compositional matrix adjust. Identities = 131/384 (34%), Positives = 204/384 (53%), Gaps = 15/384 (3%) Query: 24 ITKGVLAGGAMKLSTAIAGLIFGAWFGQILNKVGITKTIIRKAAELAGDKPXXXXXXXXX 83 I ++ GA++L +A+A IFGA QI+NK GI+++I+R AAEL GD+P Sbjct: 48 ILSNIIGAGAVRLQSAMAAAIFGAILAQIINKTGISESIVRTAAELGGDRPIIVALAITF 107 Query: 84 XXXXXXXXXNGLGMVILVGTIAIPIMLTAGLKPFXXXXXXXXXXXXXXXXXXSTWAIYTD 143 GLG VI+VGTI +PI+++ G++P +A Y Sbjct: 108 AVALIFTSLGGLGSVIMVGTIVLPILISVGIEPVIAASLLLMGLNLGGLFNVMNYAFYQS 167 Query: 144 VLKVPTDTIASYSLICAVPLIVIALIMIVYYTRKDGKVRRAWAMPLKQEFQNQDTKN--- 200 L + TI S++L + + + L++ ++ K AWAMP E Q + + Sbjct: 168 ALNLDMATIKSFALAFGI-IAAVVLLLFIFINVPKKKTVTAWAMP--TETQEEKPRASWY 224 Query: 201 --------VRSIAXXXXXXXXXXXXXXKVDIVSAVFMGALVTLLLATPKRPIHVLSSALV 252 V + ++DI+SA+ +G L +L P+ ++L+S+++ Sbjct: 225 ALLTPIIPVLLLFIWPLIFKDAEGKPIQIDIISAMLIGILYGILTTRPREIKNLLTSSII 284 Query: 253 EGIQDVXXXXXXXXXXXXXXSAVTAPQVASLIQPLIEGMIPTSPLMYILVFTLLSPLAIY 312 EGI+DV AV A+LI P+I ++PTS + Y+L F +LSPLA+Y Sbjct: 285 EGIKDVAPALGLMIGIGMLLLAVMDKTTAALISPIISKILPTSKIGYVLFFVILSPLALY 344 Query: 313 RGPLNVWGLGSGIAALIVAGGMAPVAAML-ALRIVSNLQAVSDPTNSHNVWISDYTKTDI 371 RGPLN+WGLGSG+AAL+++ G+ P A++ AL N+Q V DPTN+HNVW + +T TD Sbjct: 345 RGPLNLWGLGSGVAALMISAGILPPQAIMGALIATGNIQGVCDPTNTHNVWTAYFTNTDT 404 Query: 372 IETLRKTLPWMFVAVLISMVIAGL 395 L+KTLP+M + VL+ ++ L Sbjct: 405 TTILKKTLPYMLIVVLLDIIYVAL 428 >B7R8E1.1 B7R8E1_9THEO TRAP transporter, DctM-like membrane protein Length = 431 Score = 218 bits (554), Expect = 1e-54, Method: Compositional matrix adjust. Identities = 132/384 (34%), Positives = 199/384 (51%), Gaps = 15/384 (3%) Query: 24 ITKGVLAGGAMKLSTAIAGLIFGAWFGQILNKVGITKTIIRKAAELAGDKPXXXXXXXXX 83 I ++ GA++L A+A IFGA QI+NK GI+ I+R AAEL GD+P Sbjct: 48 IFSNIIGAGAVRLQGAMAAAIFGAILAQIINKTGISDNIVRTAAELGGDRPIAVALAITF 107 Query: 84 XXXXXXXXXNGLGMVILVGTIAIPIMLTAGLKPFXXXXXXXXXXXXXXXXXXSTWAIYTD 143 GLG VI+VGTI +PI+++ G++P +A Y Sbjct: 108 AVALIFTSLGGLGSVIMVGTIVLPILMSVGIEPVIAASLLLMGLNLGGLFNVMNYAFYQS 167 Query: 144 VLKVPTDTIASYSLICAVPLIVIALIMIVYYTRKDGKVRRAWAMPLKQEFQNQDTK---- 199 L + TI S++L + + + L++ ++ K AWAM E Q + + Sbjct: 168 ALNLDMATIKSFALAFGI-IAAVVLLLFIFINVPKKKTVAAWAM--STETQEEKPRVSWY 224 Query: 200 -------NVRSIAXXXXXXXXXXXXXXKVDIVSAVFMGALVTLLLATPKRPIHVLSSALV 252 V + ++DI+SA+ +G L +L P+ ++L+S+++ Sbjct: 225 ALLTPIIPVLLLFIWPLIFKDAEGKPIQIDIISAMLIGILYGILTTKPREIKNLLTSSII 284 Query: 253 EGIQDVXXXXXXXXXXXXXXSAVTAPQVASLIQPLIEGMIPTSPLMYILVFTLLSPLAIY 312 EGI+DV AV A+LI P+I ++PTS + Y+L F +LSPLA+Y Sbjct: 285 EGIKDVAPALGLMIGIGMLLLAVMDKTTAALISPIISKILPTSKIGYVLFFIILSPLALY 344 Query: 313 RGPLNVWGLGSGIAALIV-AGGMAPVAAMLALRIVSNLQAVSDPTNSHNVWISDYTKTDI 371 RGPLN+WGLGSG+AAL++ AG + P A M AL N+Q V DPTN+HNVW + +T TD Sbjct: 345 RGPLNLWGLGSGVAALMISAGTLPPQAIMGALIATGNIQGVCDPTNTHNVWTAYFTNTDT 404 Query: 372 IETLRKTLPWMFVAVLISMVIAGL 395 L+KTLP+M + VL+ ++ L Sbjct: 405 TTILKKTLPYMLIVVLLDIIYVAL 428 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.324 0.137 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,763,745,583 Number of extensions: 57479163 Number of successful extensions: 254218 Number of sequences better than 1.0e-05: 42 Number of HSP's gapped: 254148 Number of HSP's successfully gapped: 49 Length of query: 398 Length of database: 3,060,696,203 Length adjustment: 138 Effective length of query: 260 Effective length of database: 1,760,596,133 Effective search space: 457754994580 Effective search space used: 457754994580 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.5 bits) S2: 132 (55.5 bits)