BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0341.0032.p (267 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Q5WBZ0.1 Q5WBZ0_BACSK Two-component response regulator 234 9e-60 B3K3I8.1 B3K3I8_9BACI Response regulator receiver 231 4e-59 C5EHL9.1 C5EHL9_9FIRM Predicted protein 166 1e-39 C0D691.1 C0D691_9CLOT Putative uncharacterized protein 159 2e-37 A3CMX5.1 A3CMX5_STRSV Two-component response transcriptional reg... 157 1e-36 >Q5WBZ0.1 Q5WBZ0_BACSK Two-component response regulator Length = 249 Score = 234 bits (596), Expect = 9e-60, Method: Compositional matrix adjust. Identities = 117/252 (46%), Positives = 169/252 (67%), Gaps = 10/252 (3%) Query: 12 LFRIGGMGLHKLFLVEDEALIRSGLKHLVEEVIGGFKVVGEAENGRLALEALKSIKPDVL 71 LF GG ++K+ + EDE +IR GL +V++++ F V+GEA+NG ALE L PDVL Sbjct: 4 LFFKGGAEMYKVVIAEDEKMIRKGLVTIVDQLVTDFTVIGEADNGERALELLAHDCPDVL 63 Query: 72 ITDIRMNEMNGLEMIRRIRDQYPDLYILILSGYADFEYAKQAIKYGISDYLLKPVDRTEL 131 +TDIRM + +GL +IR R YP + I+I+SG+ +F YA+QAI++G+S YLLKP+DRTE+ Sbjct: 64 LTDIRMPKCDGLSLIREARSYYPHVKIVIVSGHEEFAYAQQAIQHGVSRYLLKPIDRTEV 123 Query: 132 AQALGEFKRKHGSGGDISDSTGEGGTDNKGRQLIRKVKELVAQRLDQEISLQYMAEQVHL 191 A E KR E ++ LI++V + +DQ+I+LQ +A+ VHL Sbjct: 124 VLAFDEIKR----------GLVEPEQEDHENHLIQQVDSYIKTHIDQDITLQAVAKLVHL 173 Query: 192 NHQYLSVLFKSETGQNFTDYVSQCRMNRAKQLLKETNLKIYEVAKLSGYLSSKHFMAVFK 251 + Y S FK+ETGQNF+ YV+Q R+ RA+ LL +TNL++YEVA++SGY + KHFM +FK Sbjct: 174 HPTYFSQWFKNETGQNFSTYVTQKRLERAETLLHQTNLRVYEVARMSGYQNEKHFMKLFK 233 Query: 252 DYTGRTPSQFRE 263 TP+QFR+ Sbjct: 234 KEKRCTPTQFRQ 245 >B3K3I8.1 B3K3I8_9BACI Response regulator receiver Length = 254 Score = 231 bits (590), Expect = 4e-59, Method: Compositional matrix adjust. Identities = 121/248 (48%), Positives = 171/248 (68%), Gaps = 6/248 (2%) Query: 22 KLFLVEDEALIRSGLKHLVEEVIGGFKVVGEAENGRLALEALKSIKPDVLITDIRMNEMN 81 KL LVEDE +IR G++ L+ V F+VV EA +G+ AL L+ DVLITDIRM EM+ Sbjct: 3 KLMLVEDEDIIRKGIRDLIGRVAPDFEVVYEAAHGKEALTYLERNVVDVLITDIRMREMD 62 Query: 82 GLEMIRRIRDQYPDLYILILSGYADFEYAKQAIKYGISDYLLKPVDRTELAQALGEFKRK 141 GL+M+ ++R ++ I+I+SGY DF YAK+A+++GI+DYLLKP+DR + AL + + Sbjct: 63 GLQMVEKLRIAGHEMPIVIISGYGDFVYAKKALQFGIADYLLKPIDRKDFTVALDKIRLT 122 Query: 142 HGS-----GGDISDSTGEGGT-DNKGRQLIRKVKELVAQRLDQEISLQYMAEQVHLNHQY 195 S GG S +GG ++GR++IRK+K +A+ D ++ LQ +AE VHLN Y Sbjct: 123 LNSSNRYAGGTEPHSEADGGPPSSEGRRMIRKLKAHIAENPDGDLRLQTLAEMVHLNPSY 182 Query: 196 LSVLFKSETGQNFTDYVSQCRMNRAKQLLKETNLKIYEVAKLSGYLSSKHFMAVFKDYTG 255 LS LFK E G N +DY+++ R+ RA +LL+ TNLK+Y++A+LSGY S KHFM VFK +TG Sbjct: 183 LSQLFKQEGGGNLSDYIAETRIRRACKLLETTNLKVYDIARLSGYQSPKHFMLVFKQHTG 242 Query: 256 RTPSQFRE 263 TP +RE Sbjct: 243 MTPGTYRE 250 >C5EHL9.1 C5EHL9_9FIRM Predicted protein Length = 254 Score = 166 bits (421), Expect = 1e-39, Method: Compositional matrix adjust. Identities = 97/254 (38%), Positives = 155/254 (61%), Gaps = 11/254 (4%) Query: 22 KLFLVEDEALIRSGLKHLVEEVIGGFKVVGEAENGRLALEALKSIKPDVLITDIRMNEMN 81 KL +V+DE IR+GL L+ G++V G E+ AL AL + DV++TDI+M E++ Sbjct: 2 KLMIVDDEPKIRNGLLKLLG-ARDGWQVTGVFEDALSALTALYDNEADVIVTDIKMPEVS 60 Query: 82 GLEMIRRIRDQYPDLYILILSGYADFEYAKQAIKYGISDYLLKPVDRTELAQALGEFKRK 141 GLE+IR+IR++ D++I+ILSG+++F YA++AI+ G++ YLLKP + EL L +++ Sbjct: 61 GLELIRQIRERNQDIHIVILSGHSNFAYAQKAIELGVTRYLLKPTNPRELVAVLEGIEKE 120 Query: 142 HGSG-GDISDST-----GEGGT----DNKGRQLIRKVKELVAQRLDQEISLQYMAEQVHL 191 G GD D GE G+ D G L++K + V I+L+ M+E++HL Sbjct: 121 LKQGAGDAPDKKQPPQEGEAGSGADGDGVGNLLVQKAIQYVEVHYGGRITLKDMSEELHL 180 Query: 192 NHQYLSVLFKSETGQNFTDYVSQCRMNRAKQLLKETNLKIYEVAKLSGYLSSKHFMAVFK 251 + YL LFK TG+N +YV++ RM +A+ L K+ +VA++ GY +K+F + FK Sbjct: 181 SPNYLCELFKRHTGKNLMEYVTEYRMMKARNYLNHVEYKVSDVAEMVGYKEAKYFSSTFK 240 Query: 252 DYTGRTPSQFREDK 265 G TP ++R + Sbjct: 241 KMYGMTPLEYRNKR 254 >C0D691.1 C0D691_9CLOT Putative uncharacterized protein Length = 253 Score = 159 bits (403), Expect = 2e-37, Method: Compositional matrix adjust. Identities = 86/246 (34%), Positives = 143/246 (58%), Gaps = 4/246 (1%) Query: 22 KLFLVEDEALIRSGLKHLVEEVIGGFKVVGEAENGRLALEALKSIKPDVLITDIRMNEMN 81 +L +++DE LIR GL+ ++E G +++ GEA +G L+ L + PD+ + D++M + N Sbjct: 3 RLVIIDDEQLIRRGLQKIIEMSGGEYEIAGEAADGEEGLKLLLKVSPDIAVVDVKMPKKN 62 Query: 82 GLEMIRRIRDQYP-DLYILILSGYADFEYAKQAIKYGISDYLLKPVDRTELAQALGE--- 137 GLEMI Q ++LS Y D+ + ++AIK GI DYLLKPV+R + + L E Sbjct: 63 GLEMIGEYLAQGAGRTRFIVLSAYDDYVFTRKAIKLGICDYLLKPVNRFDFLRQLEETAK 122 Query: 138 FKRKHGSGGDISDSTGEGGTDNKGRQLIRKVKELVAQRLDQEISLQYMAEQVHLNHQYLS 197 R+ + G +++ E + + QE++L +A++VH++ Y Sbjct: 123 LMRREAVSDGVEGGGEGAGEGAAAADAVKRAMEYIDRYFYQELTLAGVAKEVHMHPNYFG 182 Query: 198 VLFKSETGQNFTDYVSQCRMNRAKQLLKETNLKIYEVAKLSGYLSSKHFMAVFKDYTGRT 257 LFK +TG ++ Y++ RM +A++LL NLK+YEV ++ GY S KHF VFK +T T Sbjct: 183 NLFKKKTGVSYLTYLTSVRMEKARELLDNPNLKVYEVGQIVGYYSPKHFSKVFKKHTNMT 242 Query: 258 PSQFRE 263 P+++R+ Sbjct: 243 PNEYRD 248 >A3CMX5.1 A3CMX5_STRSV Two-component response transcriptional regulator (CheY-like receiver and AraC-type DNA-binding domains), putative Length = 244 Score = 157 bits (396), Expect = 1e-36, Method: Compositional matrix adjust. Identities = 96/248 (38%), Positives = 145/248 (58%), Gaps = 12/248 (4%) Query: 20 LHKLFLVEDEALIRSGLKHLVEEVIGGFKVVGEAENGRLALEALKSIKPDVLITDIRMNE 79 ++ + +VEDE L+R G+ LV+ G +V+ +AENG A + + D+L+TDI M + Sbjct: 1 MYSIIIVEDEYLVRQGIASLVDYEQFGMQVIAQAENGIEAWQKFQENPADILLTDINMPQ 60 Query: 80 MNGLEMIRRIRDQYPDLYILILSGYADFEYAKQAIKYGISDYLLKPVDRTELAQALGEFK 139 MNGLE+ + +RDQ P +I+ L+GY DF+YA+ AIK G DYLLKP + ++ + L + + Sbjct: 61 MNGLELAKLVRDQAPKCHIVFLTGYDDFDYARTAIKLGADDYLLKPFSKDDVEEMLAKVQ 120 Query: 140 R---KHGSGGDISDSTGEGGTDNKGRQLIRKVKELVAQRL-DQEISLQYMAEQVHLNHQY 195 K I + +G +++E + RL D E+SL+ +A Q+ + Y Sbjct: 121 TKLDKERKKAQIQNLVDQGHH--------SELEEAIHARLADSELSLKSLAFQLGFSPSY 172 Query: 196 LSVLFKSETGQNFTDYVSQCRMNRAKQLLKETNLKIYEVAKLSGYLSSKHFMAVFKDYTG 255 LSVL K E G F DY+ Q RM +AK LL T+LKIYE+A+ G+ +F FK G Sbjct: 173 LSVLIKKELGLPFQDYLIQERMKKAKLLLLTTDLKIYEIAEQVGFEDMNYFSQRFKQVVG 232 Query: 256 RTPSQFRE 263 TP QF++ Sbjct: 233 LTPRQFKK 240 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.318 0.137 0.383 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,574,226,433 Number of extensions: 64504649 Number of successful extensions: 312557 Number of sequences better than 1.0e-05: 15997 Number of HSP's gapped: 322052 Number of HSP's successfully gapped: 16513 Length of query: 267 Length of database: 3,060,696,203 Length adjustment: 133 Effective length of query: 134 Effective length of database: 1,807,701,208 Effective search space: 242231961872 Effective search space used: 242231961872 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 130 (54.7 bits)