BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0341.0006.p (505 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K5R4.1 B3K5R4_9BACI GerA spore germination protein 509 e-142 B3K743.1 B3K743_9BACI GerA spore germination protein 483 e-134 B1DF37.1 B1DF37_9BACL GerA spore germination protein 483 e-134 B4BK05.1 B4BK05_9BACI GerA spore germination protein 471 e-131 Q5KZH7.1 Q5KZH7_GEOKA Spore germination protein 471 e-131 >B3K5R4.1 B3K5R4_9BACI GerA spore germination protein Length = 531 Score = 509 bits (1310), Expect = e-142, Method: Compositional matrix adjust. Identities = 261/504 (51%), Positives = 333/504 (66%), Gaps = 7/504 (1%) Query: 3 SGGCDLKDTNFATLSSCLKENQQQLVQLFGKSTDLVIKELQPDPCTQ--IAVIYIDGLVD 60 S KD+N LS L+ N + + G STD+VI+E+ D + +AV+Y DGL D Sbjct: 20 SAAATKKDSN--ALSPELESNLNHIRRTLGNSTDIVIREIPLDEGEKHYMAVVYTDGLAD 77 Query: 61 TQVLHNSILFSLQEGCTPDRLKALDAGQKLE---LLHKRILMAGDISVTRDLEPFVHQLL 117 +++ SI+ ++ + + + G+ LE +L + ++ +I D + H LL Sbjct: 78 QKIISESIMSAVADIAKSRARENTEYGKPLEAQKVLEEISFISKEIQSISDYKTLFHSLL 137 Query: 118 SGNVMVLVDGTSSALRIGLPGWEDRNVSEPSSQTVVRGPMEGFTENLRTNTALIRRKIKD 177 SG+ +VLVDG + G GW+DR V E S++ VVRGP E FTE +RTNTALIRRKIKD Sbjct: 138 SGDAIVLVDGLDRGIAAGARGWKDRGVMETSAENVVRGPRESFTEAVRTNTALIRRKIKD 197 Query: 178 PQLWLETLQIGRVTQTSVSIMYLTHIANPDLVQEVKRRLDKIDTDSILESGYIEEFIQDK 237 P LW+ET QIGR+TQT V +MY+ IAN +V EV+ RL++ID DSILESGYIEE +QDK Sbjct: 198 PHLWMETKQIGRITQTDVVMMYIDGIANEKIVGEVRTRLERIDVDSILESGYIEELVQDK 257 Query: 238 AATPFPTVYNSDRPDTIAAGILEGKVAILVDGTPFVLLVPTFFVSFFQSAEDYYQRADIA 297 TPFPT+YN++RPD +AA +LEGK+AI+VDGTPFVL+VP FVSF +AEDYYQRADI+ Sbjct: 258 TLTPFPTIYNTERPDVVAAELLEGKIAIIVDGTPFVLIVPALFVSFLHAAEDYYQRADIS 317 Query: 298 XXXXXXXXXXXXXXXXAPSFYVAVTTYHQEMIPTTLVISLAAQRESVPFPAFVEVILMEL 357 PS YVA+TT+H EM+PT L+ LA QRE +PFPAFVE +LME+ Sbjct: 318 SFLRILRYLSIFISLLGPSLYVAITTFHHEMLPTRLLFGLAGQREQIPFPAFVEAVLMEV 377 Query: 358 TYEILREAGVRIPKNIGQAISIVGTLVIGQAAVSAGFISSAMVIIVSITAISSFVIPETG 417 T+EILREAGVR+PKNIG AISIVGTLVIGQAAV AG IS AMVI+V+ITAISSFV+P Sbjct: 378 TFEILREAGVRLPKNIGSAISIVGTLVIGQAAVEAGIISPAMVIVVAITAISSFVVPSFN 437 Query: 418 MXXXXXXXXXXXXXXXXXXGLYGILFGVFIIVMHLASLRSFGMPYMSPIGPYQSKDLKDS 477 M GL+GIL GV I++HL SLRSFG+PYMSP P + KDS Sbjct: 438 MSIAFRMLRFPLMALAGSFGLFGILVGVIAIILHLCSLRSFGIPYMSPFAPLIPEGQKDS 497 Query: 478 LFRFPWPFLKSRPTNSKIQNPNRQ 501 + R P L +RP N R+ Sbjct: 498 ILRLPHWMLINRPRLINQDNEKRR 521 >B3K743.1 B3K743_9BACI GerA spore germination protein Length = 532 Score = 483 bits (1244), Expect = e-134, Method: Compositional matrix adjust. Identities = 251/492 (51%), Positives = 325/492 (66%), Gaps = 2/492 (0%) Query: 16 LSSCLKENQQQLVQLFGKSTDLVIKELQP--DPCTQIAVIYIDGLVDTQVLHNSILFSLQ 73 LS L N ++ + G S D++I+ L +AVIYIDGLVDT +++SI+ SL Sbjct: 32 LSDRLDHNLHRIREQTGHSRDIIIRTLSHMFGSDHTLAVIYIDGLVDTLTINHSIMQSLT 91 Query: 74 EGCTPDRLKALDAGQKLELLHKRILMAGDISVTRDLEPFVHQLLSGNVMVLVDGTSSALR 133 + L D + L ++ R L G IS +EP + +L GN ++L+DG+S + Sbjct: 92 SKDYDEELHTSDPAEILSIIKNRFLSIGSISDLEHMEPLISAILEGNTVILIDGSSHGVI 151 Query: 134 IGLPGWEDRNVSEPSSQTVVRGPMEGFTENLRTNTALIRRKIKDPQLWLETLQIGRVTQT 193 G E+R V EP SQTVVRGP EGFTEN+RTNTALIRRKIK P LW+ +IGRVTQT Sbjct: 152 ASSIGGEERGVEEPQSQTVVRGPKEGFTENIRTNTALIRRKIKSPNLWIVDRKIGRVTQT 211 Query: 194 SVSIMYLTHIANPDLVQEVKRRLDKIDTDSILESGYIEEFIQDKAATPFPTVYNSDRPDT 253 V++MYL IAN +VQE+ RLD+IDTDSILESGYIEEFIQD TPFPT+ N++RPD Sbjct: 212 DVAVMYLNGIANDKIVQEILNRLDRIDTDSILESGYIEEFIQDTTFTPFPTITNTERPDA 271 Query: 254 IAAGILEGKVAILVDGTPFVLLVPTFFVSFFQSAEDYYQRADIAXXXXXXXXXXXXXXXX 313 IA ILEGK+AILVDGTPF L+ P FQS+EDYYQ+ DIA Sbjct: 272 IAGAILEGKIAILVDGTPFALIAPITIFKLFQSSEDYYQKFDIATFLRLLRIISFVVSML 331 Query: 314 APSFYVAVTTYHQEMIPTTLVISLAAQRESVPFPAFVEVILMELTYEILREAGVRIPKNI 373 PS Y+A++T+HQEM+PTTL+ISLAAQRE +PFPA VE + ME+T+E+LREAGVR+P+ + Sbjct: 332 LPSLYIAISTFHQEMLPTTLLISLAAQREGIPFPAVVEALAMEITFEVLREAGVRMPRAM 391 Query: 374 GQAISIVGTLVIGQAAVSAGFISSAMVIIVSITAISSFVIPETGMXXXXXXXXXXXXXXX 433 G AISIVG LV+GQAAV AG +S+AMVIIV+ TAI+SFV P + Sbjct: 392 GSAISIVGALVLGQAAVQAGLVSAAMVIIVAFTAIASFVAPSVSIANSARLLRFGFMILA 451 Query: 434 XXXGLYGILFGVFIIVMHLASLRSFGMPYMSPIGPYQSKDLKDSLFRFPWPFLKSRPTNS 493 GL+GI+ G+ +++HLA LRSFG+PY++P P+ D KD L R PW + +RPT Sbjct: 452 ATLGLFGIMAGLLAMLIHLAGLRSFGLPYLTPFAPFIPLDQKDVLVRVPWWAMVTRPTLI 511 Query: 494 KIQNPNRQGTSK 505 +N RQ ++ Sbjct: 512 NRKNAKRQKMNQ 523 >B1DF37.1 B1DF37_9BACL GerA spore germination protein Length = 486 Score = 483 bits (1242), Expect = e-134, Method: Compositional matrix adjust. Identities = 253/479 (52%), Positives = 321/479 (67%), Gaps = 15/479 (3%) Query: 31 FGKSTDLVIKELQ---PDPCTQIAVIYIDGLVDTQVLHNSILFSLQEGCTPDRLKALDAG 87 G S D+V KE Q D ++ + Y DGLV+ + + IL +L LD Sbjct: 13 LGNSNDIVFKEFQIEYADRSQRVDLFYADGLVNAGSVQDFILETLMV-----EFPKLDTN 67 Query: 88 QKLELLHKRILMAGDISVTRDLEPFVHQLLSGNVMVLVDGTSSALRIGLPGWEDRNVSEP 147 Q L +L AGD+ E + L SG+V+++ D T L I + G +DR VSE Sbjct: 68 QHPFL---DLLTAGDVEEVTSTETMYYDLFSGHVILIWDSTK-ILSIAMNGIKDRAVSEA 123 Query: 148 SSQTVVRGPMEGFTENLRTNTALIRRKIKDPQLWLETLQIGRVTQTSVSIMYLTHIANPD 207 ++TV RGP E FTE++RTNTAL+RRKIK+P LWLE ++IG+VTQTSVSIMY+ I + Sbjct: 124 MTETVSRGPKESFTESIRTNTALVRRKIKNPNLWLENMRIGKVTQTSVSIMYVKGIVSDK 183 Query: 208 LVQEVKRRLDKIDTDSILESGYIEEFIQDKAATPFPTVYNSDRPDTIAAGILEGKVAILV 267 +V EV+ RL +ID D ILESGYIEE IQD A +PFPT+YNS+RPD IAA +LEGKVAILV Sbjct: 184 IVDEVRNRLTRIDIDGILESGYIEELIQDSAYSPFPTMYNSERPDVIAAELLEGKVAILV 243 Query: 268 DGTPFVLLVPTFFVSFFQSAEDYYQRADIAXXXXXXXXXXXXXXXXAPSFYVAVTTYHQE 327 DGTP VL+VP F+SF SAEDYYQRADI+ PS Y+A+TT+HQE Sbjct: 244 DGTPHVLVVPALFISFMHSAEDYYQRADISSLIRILRFVGIFIALLGPSLYIAITTFHQE 303 Query: 328 MIPTTLVISLAAQRESVPFPAFVEVILMELTYEILREAGVRIPKNIGQAISIVGTLVIGQ 387 M+PT L+I LAAQRE +PFPAF+E ++ME+T+EILREAGVR+P+ +GQAISIVGTLVIGQ Sbjct: 304 MLPTQLLIGLAAQREGIPFPAFIEALIMEITFEILREAGVRMPRTMGQAISIVGTLVIGQ 363 Query: 388 AAVSAGFISSAMVIIVSITAISSFVIPETGMXXXXXXXXXXXXXXXXXXGLYGILFGVFI 447 AAV AG +S+AMVI+VSITAISSFV+P M GLYGI+ G+F Sbjct: 364 AAVDAGIVSAAMVIVVSITAISSFVVPAMNMSISIRMIRFALMGLAASFGLYGIILGIFA 423 Query: 448 IVMHLASLRSFGMPYMSPIGPYQSKDLKDSLFRFP-WPFLKSRPTNSKIQNPNRQGTSK 505 +V+HL SLR+FG+PYMSP GP+ ++D KD+LFR P W K S QN +G +K Sbjct: 424 LVLHLCSLRTFGVPYMSPFGPFIAEDTKDTLFRLPHWAMRKRLRLIS--QNNITRGNNK 480 >B4BK05.1 B4BK05_9BACI GerA spore germination protein Length = 548 Score = 471 bits (1213), Expect = e-131, Method: Compositional matrix adjust. Identities = 232/488 (47%), Positives = 327/488 (67%), Gaps = 5/488 (1%) Query: 16 LSSCLKENQQQLVQLFGKSTDLVIKE--LQPDPCTQIAVIYIDGLVDTQVLHNSILFSLQ 73 +S+ L N + + G+S D+VI+ L + + AV+++DGLVD + ++ +L L Sbjct: 44 MSAELAVNIDIIRRATGESDDVVIRRFSLGQEKQMKAAVVFVDGLVDEKNVYEFLLTPLL 103 Query: 74 EGCTPDRLKALDAGQKLELLHKRILMAGDISVTRDLEPFVHQLLSGNVMVLVDGTSSALR 133 P AL+ ++L+ + +++ G + D + +L SG ++++DGTS A+ Sbjct: 104 TASFP---LALEEKERLQWIEQKLAAVGGVKHVADWKLLFCELFSGATVMMIDGTSFAIC 160 Query: 134 IGLPGWEDRNVSEPSSQTVVRGPMEGFTENLRTNTALIRRKIKDPQLWLETLQIGRVTQT 193 G + R++ EP +Q VRGP EGFTE+LRTNTA+IRR+IK+P LWLET+QIG VTQT Sbjct: 161 ASTKGGQYRSIEEPQTQIAVRGPREGFTESLRTNTAMIRRRIKNPNLWLETMQIGTVTQT 220 Query: 194 SVSIMYLTHIANPDLVQEVKRRLDKIDTDSILESGYIEEFIQDKAATPFPTVYNSDRPDT 253 V+IMY+ IAN ++++EVK+RL +ID D +LESGYIE+ I+D+ TPFPT Y+++RPD Sbjct: 221 DVAIMYVKGIANDEIIEEVKKRLRRIDIDGVLESGYIEQLIEDQTFTPFPTTYHTERPDI 280 Query: 254 IAAGILEGKVAILVDGTPFVLLVPTFFVSFFQSAEDYYQRADIAXXXXXXXXXXXXXXXX 313 +AA +LEG+VAI V+GTPFVL+ P F+ FFQ+ EDYY R DIA Sbjct: 281 VAANLLEGRVAIFVEGTPFVLVAPALFIQFFQAVEDYYARFDIATALRFLRVLIFFISLV 340 Query: 314 APSFYVAVTTYHQEMIPTTLVISLAAQRESVPFPAFVEVILMELTYEILREAGVRIPKNI 373 AP+ Y+A T +HQEMIPT LVI++AAQRE+VPFPAFVE ++ME+T+EILREAG+R+P+ + Sbjct: 341 APAAYIAGTMFHQEMIPTLLVIAIAAQREAVPFPAFVEALIMEVTFEILREAGIRLPRAV 400 Query: 374 GQAISIVGTLVIGQAAVSAGFISSAMVIIVSITAISSFVIPETGMXXXXXXXXXXXXXXX 433 GQA+SIVG LVIGQAAV AG +SSAMVI+VSITAI+SF P + Sbjct: 401 GQAVSIVGALVIGQAAVEAGLVSSAMVIVVSITAIASFATPSFAIAISARLIRFALMFLA 460 Query: 434 XXXGLYGILFGVFIIVMHLASLRSFGMPYMSPIGPYQSKDLKDSLFRFPWPFLKSRPTNS 493 G YGI+ G+ ++ +HL SLRSFG+PYMSP+ P+ ++ D+LFR P + RP Sbjct: 461 AMFGFYGIMMGLLVMTIHLCSLRSFGVPYMSPLAPFMPSNMGDTLFRLPTWMFRERPRLI 520 Query: 494 KIQNPNRQ 501 +N RQ Sbjct: 521 SQKNIVRQ 528 >Q5KZH7.1 Q5KZH7_GEOKA Spore germination protein Length = 539 Score = 471 bits (1212), Expect = e-131, Method: Compositional matrix adjust. Identities = 233/488 (47%), Positives = 322/488 (65%), Gaps = 5/488 (1%) Query: 16 LSSCLKENQQQLVQLFGKSTDLVIKELQPDPCTQI--AVIYIDGLVDTQVLHNSILFSLQ 73 LS+ L N + Q G+S D+VI+ QI A+I++DGLVD + ++ +L L Sbjct: 35 LSAELAVNIDIIRQKTGESNDVVIRRFALGQEWQIKAAIIFVDGLVDEKNVYEFVLTPLL 94 Query: 74 EGCTPDRLKALDAGQKLELLHKRILMAGDISVTRDLEPFVHQLLSGNVMVLVDGTSSALR 133 + P AL+ ++ + + K++ G + D +L SG ++L+DG A+ Sbjct: 95 QASFP---LALEEKERFQWIEKKLAAVGGVKHISDWRKLFFELFSGATVMLIDGVPFAIS 151 Query: 134 IGLPGWEDRNVSEPSSQTVVRGPMEGFTENLRTNTALIRRKIKDPQLWLETLQIGRVTQT 193 G E R++ EP +Q VRGP EGFTE+LRTNTA+IRR+I++P LWLET+QIG VTQT Sbjct: 152 ASTKGGEYRSIEEPQTQIAVRGPREGFTESLRTNTAMIRRRIRNPNLWLETMQIGAVTQT 211 Query: 194 SVSIMYLTHIANPDLVQEVKRRLDKIDTDSILESGYIEEFIQDKAATPFPTVYNSDRPDT 253 V+IMY+ IAN ++V+EV+ RL +ID D +LESGYIE+ I+D+ T FPT Y+++RPD Sbjct: 212 DVAIMYIKGIANDEIVKEVRARLRRIDIDGVLESGYIEQLIEDQTFTTFPTTYHTERPDI 271 Query: 254 IAAGILEGKVAILVDGTPFVLLVPTFFVSFFQSAEDYYQRADIAXXXXXXXXXXXXXXXX 313 +AA +LEG+VA+ V+GTPFVL+ P F+ FFQ+ EDYY R DI+ Sbjct: 272 VAANLLEGRVAVFVEGTPFVLVAPALFIQFFQAVEDYYARFDISTALRFLRVLIFFISLV 331 Query: 314 APSFYVAVTTYHQEMIPTTLVISLAAQRESVPFPAFVEVILMELTYEILREAGVRIPKNI 373 AP+ Y+A TT+HQEMIPT LVI++AAQRE+VPFP FVE ++ME+T+EILREAGVR+P+ + Sbjct: 332 APAIYIAATTFHQEMIPTQLVIAIAAQREAVPFPGFVEALVMEVTFEILREAGVRLPRAV 391 Query: 374 GQAISIVGTLVIGQAAVSAGFISSAMVIIVSITAISSFVIPETGMXXXXXXXXXXXXXXX 433 GQA+SIVG LVIGQAAV AGF+SSAMVI+VSITAI+SF P + Sbjct: 392 GQAVSIVGALVIGQAAVEAGFVSSAMVIVVSITAIASFATPSFAIAISARLIRFGLMFLA 451 Query: 434 XXXGLYGILFGVFIIVMHLASLRSFGMPYMSPIGPYQSKDLKDSLFRFPWPFLKSRPTNS 493 G YGI+ G+ ++ +HL SLRSFG+PYMSP+ P+ ++ D+LFR P + RP Sbjct: 452 AMFGFYGIMMGILVMTIHLCSLRSFGVPYMSPLAPFIPTNMGDTLFRLPTWMFRERPRLI 511 Query: 494 KIQNPNRQ 501 +N RQ Sbjct: 512 NQKNIVRQ 519 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.321 0.137 0.393 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,652,252,539 Number of extensions: 100403850 Number of successful extensions: 243181 Number of sequences better than 1.0e-05: 1094 Number of HSP's gapped: 239753 Number of HSP's successfully gapped: 1105 Length of query: 505 Length of database: 3,060,696,203 Length adjustment: 140 Effective length of query: 365 Effective length of database: 1,741,754,103 Effective search space: 635740247595 Effective search space used: 635740247595 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 133 (55.8 bits)