BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0341.0003.p (500 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value C3E5D0.1 C3E5D0_BACTU Uncharacterized membrane protein yndD 428 e-118 C3IVC2.1 C3IVC2_BACTU Uncharacterized membrane protein yndD 427 e-117 B7IMM8.1 B7IMM8_BACC2 Spore germination protein GerAA 426 e-117 B9J5D8.1 B9J5D8_BACCQ Spore germination protein 426 e-117 B5V279.1 B5V279_BACCE Spore germination protein KA 426 e-117 >C3E5D0.1 C3E5D0_BACTU Uncharacterized membrane protein yndD Length = 527 Score = 428 bits (1100), Expect = e-118, Method: Compositional matrix adjust. Identities = 219/490 (44%), Positives = 316/490 (64%), Gaps = 6/490 (1%) Query: 13 FEANLDYLKKTLDHLPDIIFKKMHVGQPGIQIAMVYLSGIADADMLNEHIVKPLI--LTG 70 F +NL+ +K+ + H D+ F+K ++G+ IQ +V++ G++D +++++HI+K L+ + Sbjct: 41 FASNLELIKQQIGHNSDVRFRKFNIGRTDIQAGIVFVDGLSDKEIIDKHIMKLLMDDFSD 100 Query: 71 IRERHSQFNPEKLKEELIQQTAFTIN-TKEAADIEACVIEVLSGNTCLLIEGNEEFFVVS 129 + S + + +E I+ TI+ E I+ + +VL G+T LLI+G + F++ Sbjct: 101 EYKNESSYVEGTVSKEYIKNKVLTISEVAEVHYIKDLIPKVLIGSTALLIDGLADVFILG 160 Query: 130 TGKFPLRSVEEPNTETLVRGPREGFIEDLESNLAMIRRHIKDLSFKIDKIRIGRRSSRKL 189 T K R++EEP +E LVRGPR GF E L N +++RRH D S + K+ +G R+ ++L Sbjct: 161 TTKANKRNIEEPVSEALVRGPRVGFTEVLSDNTSLLRRHCADESLSLVKLPVGERAKKEL 220 Query: 190 AVLYISDIANPKLVQEVKRRVETIDVDDASDTGIIEQWIEESSWSPFPQIQASERSDKAI 249 V +I D+A+ +LV E+K R++ ID+D ++G IEQ IE++ SPFPQIQ +ER D+ I Sbjct: 221 VVAFIKDLASTELVDEIKNRIQKIDIDSVLESGYIEQLIEDNYLSPFPQIQNTERPDRVI 280 Query: 250 TALMSGRVVILLDGTPFALIAPMTFWMLAQSPEDYYEXXXXXXXXXXXXXXXXXXXXXXP 309 +ALM GRV ILLDGTPF LIAP+TF ML QSPEDYYE P Sbjct: 281 SALMEGRVAILLDGTPFVLIAPVTFSMLLQSPEDYYERWLPGTLLRLLRFMTAFVSLFAP 340 Query: 310 SLYVALISFHPGLIPPELVISIISSREGIPFPVFVEALIMEVSLEILREASLRLPKXXXX 369 +LY++ ISFHPGLIP +L ISI+ SREG+PFP +EALIMEV++E+LREA LRLPK Sbjct: 341 ALYISFISFHPGLIPTKLAISILGSREGVPFPALIEALIMEVAIEVLREAGLRLPKPIGP 400 Query: 370 XXXXXXXXXXXXXXXEAAVVSSFLIIIVGLTAIASFASPQYSGSIGIRLLRFPIMIIAAI 429 EA +VS ++I+V +TAI+SF PQYS I +R+LRF M AAI Sbjct: 401 AMGIVGGLIIGQAAVEAGIVSPIMVIVVAVTAISSFTIPQYSVGITLRILRFVAMFCAAI 460 Query: 430 YGLYGVILAFIFLGAHMVQIKSFGVPYMSPLAPIRLRDQQDTLVRVPFKYQKKRPIMLKT 489 +GLYGVIL F+ L +H+V++KSFGVPY SP P R D +D +VR+P K ++RP ++ T Sbjct: 461 FGLYGVILFFLLLCSHLVKLKSFGVPYTSPAVPYRFSDWKDFMVRMPLKIMRRRPKIMHT 520 Query: 490 QDETSANRKR 499 + NRKR Sbjct: 521 K---KTNRKR 527 >C3IVC2.1 C3IVC2_BACTU Uncharacterized membrane protein yndD Length = 521 Score = 427 bits (1098), Expect = e-117, Method: Compositional matrix adjust. Identities = 220/490 (44%), Positives = 315/490 (64%), Gaps = 6/490 (1%) Query: 13 FEANLDYLKKTLDHLPDIIFKKMHVGQPGIQIAMVYLSGIADADMLNEHIVKPLI--LTG 70 F +NL+ +K+ + H D+ F+K ++G+ IQ +V++ G++D +++++HI+K L+ + Sbjct: 35 FASNLELIKQQIGHNSDVRFRKFNIGRTDIQAGIVFVDGLSDKEIIDKHIMKLLMDDFSD 94 Query: 71 IRERHSQFNPEKLKEELIQQTAFTIN-TKEAADIEACVIEVLSGNTCLLIEGNEEFFVVS 129 + + + + +E I+ TI+ E I+ + +VL G+T LLI+G + F++ Sbjct: 95 EYKNETSYVEGTVSKEYIKNKVLTISEVAEVHYIKDLIPKVLIGSTALLIDGLADVFILG 154 Query: 130 TGKFPLRSVEEPNTETLVRGPREGFIEDLESNLAMIRRHIKDLSFKIDKIRIGRRSSRKL 189 T K R++EEP +E LVRGPR GF E L N +++RRH D S + K+ +G R+ ++L Sbjct: 155 TTKANKRNIEEPVSEALVRGPRVGFTEVLSDNTSLLRRHCADESLSLVKLPVGERAKKEL 214 Query: 190 AVLYISDIANPKLVQEVKRRVETIDVDDASDTGIIEQWIEESSWSPFPQIQASERSDKAI 249 V +I D+A+ +LV EVK R++ ID+D ++G IEQ IE++ SPFPQIQ +ER D+ I Sbjct: 215 VVAFIRDLASTELVDEVKNRIQKIDIDSVLESGYIEQLIEDNYLSPFPQIQNTERPDRVI 274 Query: 250 TALMSGRVVILLDGTPFALIAPMTFWMLAQSPEDYYEXXXXXXXXXXXXXXXXXXXXXXP 309 +ALM GRV ILLDGTPF LIAP+TF ML QSPEDYYE P Sbjct: 275 SALMEGRVAILLDGTPFVLIAPVTFSMLLQSPEDYYERWLPGTLLRLLRFMTAFVSLFAP 334 Query: 310 SLYVALISFHPGLIPPELVISIISSREGIPFPVFVEALIMEVSLEILREASLRLPKXXXX 369 +LY++ ISFHPGLIP +L ISII SREG+PFP +EALIMEV++E+LREA LRLPK Sbjct: 335 ALYISFISFHPGLIPTKLAISIIGSREGVPFPALIEALIMEVAIEVLREAGLRLPKPIGP 394 Query: 370 XXXXXXXXXXXXXXXEAAVVSSFLIIIVGLTAIASFASPQYSGSIGIRLLRFPIMIIAAI 429 EA +VS ++I+V +TAI+SF PQYS I +R+LRF M AAI Sbjct: 395 AMGIVGGLIIGQAAVEAGIVSPIMVIVVAVTAISSFTIPQYSVGITLRILRFVAMFCAAI 454 Query: 430 YGLYGVILAFIFLGAHMVQIKSFGVPYMSPLAPIRLRDQQDTLVRVPFKYQKKRPIMLKT 489 +GLYGVIL F+ L +H+V++KSFGVPY SP P R D +D +VR+P K ++RP ++ T Sbjct: 455 FGLYGVILFFLLLCSHLVKLKSFGVPYTSPAVPYRFSDWKDFMVRMPLKIMRRRPKIMHT 514 Query: 490 QDETSANRKR 499 Q RKR Sbjct: 515 QKNI---RKR 521 >B7IMM8.1 B7IMM8_BACC2 Spore germination protein GerAA Length = 524 Score = 426 bits (1096), Expect = e-117, Method: Compositional matrix adjust. Identities = 220/490 (44%), Positives = 315/490 (64%), Gaps = 6/490 (1%) Query: 13 FEANLDYLKKTLDHLPDIIFKKMHVGQPGIQIAMVYLSGIADADMLNEHIVKPLI--LTG 70 F +NL+ +K+ + H D+ F+K ++G+ IQ +V++ G++D +++++HI+K L+ + Sbjct: 38 FASNLELIKQQIGHNSDVRFRKFNIGRTDIQAGIVFVDGLSDKEIIDKHIMKLLMDDFSD 97 Query: 71 IRERHSQFNPEKLKEELIQQTAFTIN-TKEAADIEACVIEVLSGNTCLLIEGNEEFFVVS 129 + + + + +E I+ TI+ E I+ + +VL G+T LLI+G + F++ Sbjct: 98 EYKNETSYVEGTVSKEYIKNKVLTISEVAEVHYIKDLIPKVLIGSTVLLIDGLADVFILG 157 Query: 130 TGKFPLRSVEEPNTETLVRGPREGFIEDLESNLAMIRRHIKDLSFKIDKIRIGRRSSRKL 189 T K R++EEP +E LVRGPR GF E L N +++RRH D S + K+ +G R+ ++L Sbjct: 158 TTKANKRNIEEPVSEALVRGPRVGFTEVLSDNTSLLRRHCADESLSLVKLPVGERAKKEL 217 Query: 190 AVLYISDIANPKLVQEVKRRVETIDVDDASDTGIIEQWIEESSWSPFPQIQASERSDKAI 249 V +I D+A+ +LV EVK R++ ID+D ++G IEQ IE++ SPFPQIQ +ER D+ I Sbjct: 218 VVAFIRDLASTELVDEVKNRIQKIDIDSVLESGYIEQLIEDNYLSPFPQIQNTERPDRVI 277 Query: 250 TALMSGRVVILLDGTPFALIAPMTFWMLAQSPEDYYEXXXXXXXXXXXXXXXXXXXXXXP 309 +ALM GRV ILLDGTPF LIAP+TF ML QSPEDYYE P Sbjct: 278 SALMEGRVAILLDGTPFVLIAPVTFSMLLQSPEDYYERWLPGTLLRLLRFMTAFVSLFAP 337 Query: 310 SLYVALISFHPGLIPPELVISIISSREGIPFPVFVEALIMEVSLEILREASLRLPKXXXX 369 +LY++ ISFHPGLIP +L ISII SREG+PFP +EALIMEV++E+LREA LRLPK Sbjct: 338 ALYISFISFHPGLIPTKLAISIIGSREGVPFPALIEALIMEVAIEVLREAGLRLPKPIGP 397 Query: 370 XXXXXXXXXXXXXXXEAAVVSSFLIIIVGLTAIASFASPQYSGSIGIRLLRFPIMIIAAI 429 EA +VS ++I+V +TAI+SF PQYS I +R+LRF M AAI Sbjct: 398 AMGIVGGLIIGQAAVEAGIVSPIMVIVVAVTAISSFTIPQYSVGITLRILRFVAMFCAAI 457 Query: 430 YGLYGVILAFIFLGAHMVQIKSFGVPYMSPLAPIRLRDQQDTLVRVPFKYQKKRPIMLKT 489 +GLYGVIL F+ L +H+V++KSFGVPY SP P R D +D +VR+P K ++RP ++ T Sbjct: 458 FGLYGVILFFLLLCSHLVKLKSFGVPYTSPAVPYRFSDWKDFMVRMPLKIMRRRPKIMHT 517 Query: 490 QDETSANRKR 499 Q RKR Sbjct: 518 QKNI---RKR 524 >B9J5D8.1 B9J5D8_BACCQ Spore germination protein Length = 521 Score = 426 bits (1095), Expect = e-117, Method: Compositional matrix adjust. Identities = 215/481 (44%), Positives = 313/481 (65%), Gaps = 3/481 (0%) Query: 13 FEANLDYLKKTLDHLPDIIFKKMHVGQPGIQIAMVYLSGIADADMLNEHIVKPLI--LTG 70 F +NL+ +K+ + H D+ F+K ++G+ IQ +V++ G++D +++++HI+K L+ + Sbjct: 35 FASNLELIKQQIGHNSDVRFRKFNIGRTDIQAGIVFVDGLSDKEIIDKHIMKLLMDDFSD 94 Query: 71 IRERHSQFNPEKLKEELIQQTAFTIN-TKEAADIEACVIEVLSGNTCLLIEGNEEFFVVS 129 + S + + +E I+ TI+ E I+ + +VL G+T LLI+G + F++ Sbjct: 95 EYKNESSYVERTVSKEYIKNKVLTISEVAEVHYIKDLIPKVLIGSTALLIDGLADVFILG 154 Query: 130 TGKFPLRSVEEPNTETLVRGPREGFIEDLESNLAMIRRHIKDLSFKIDKIRIGRRSSRKL 189 T K R++EEP +E LVRGPR GF E L N +++RRH D S + K+ +G R+ ++L Sbjct: 155 TTKANKRNIEEPVSEALVRGPRVGFTEVLSDNTSLLRRHCADESLSLVKLPVGERAKKEL 214 Query: 190 AVLYISDIANPKLVQEVKRRVETIDVDDASDTGIIEQWIEESSWSPFPQIQASERSDKAI 249 V +I+D+A+ +LV E+K R++ ID+D ++G IEQ IE++ SPFPQIQ +ER D+ I Sbjct: 215 VVAFINDLASTELVDEIKNRIQKIDIDSVLESGYIEQLIEDNYLSPFPQIQNTERPDRVI 274 Query: 250 TALMSGRVVILLDGTPFALIAPMTFWMLAQSPEDYYEXXXXXXXXXXXXXXXXXXXXXXP 309 +ALM GRV ILLDGTPF LIAP+TF ML QSPEDYYE P Sbjct: 275 SALMEGRVAILLDGTPFVLIAPVTFSMLLQSPEDYYERWLPGTLLRLLRFMTAFVSLFAP 334 Query: 310 SLYVALISFHPGLIPPELVISIISSREGIPFPVFVEALIMEVSLEILREASLRLPKXXXX 369 +LY++ ISFHPGLIP +L ISII SREG+PFP +EALIME+++E+LREA LRLPK Sbjct: 335 ALYISFISFHPGLIPTKLAISIIGSREGVPFPALIEALIMEIAIEVLREAGLRLPKPIGP 394 Query: 370 XXXXXXXXXXXXXXXEAAVVSSFLIIIVGLTAIASFASPQYSGSIGIRLLRFPIMIIAAI 429 EA +VS ++I+V +TAI+SF PQYS I +R+LRF M AAI Sbjct: 395 AMGIVGGLIIGQAAVEAGIVSPIMVIVVAVTAISSFTIPQYSVGITLRILRFVAMFCAAI 454 Query: 430 YGLYGVILAFIFLGAHMVQIKSFGVPYMSPLAPIRLRDQQDTLVRVPFKYQKKRPIMLKT 489 +GLYGVIL F+ L +H+V++KSFGVPY SP P R D +D +VR+P K ++RP ++ T Sbjct: 455 FGLYGVILFFLLLCSHLVKLKSFGVPYTSPAVPYRFSDWKDFMVRMPLKIMRRRPKIMHT 514 Query: 490 Q 490 + Sbjct: 515 K 515 >B5V279.1 B5V279_BACCE Spore germination protein KA Length = 531 Score = 426 bits (1095), Expect = e-117, Method: Compositional matrix adjust. Identities = 217/481 (45%), Positives = 312/481 (64%), Gaps = 3/481 (0%) Query: 13 FEANLDYLKKTLDHLPDIIFKKMHVGQPGIQIAMVYLSGIADADMLNEHIVKPLI--LTG 70 F +NL+ +K+ + H D+ F+K ++G+ IQ +V++ G++D +++++HI+K L+ + Sbjct: 38 FASNLELIKQQIGHNSDVRFRKFNIGRTDIQAGIVFVDGLSDKEIIDKHIMKLLMDDFSD 97 Query: 71 IRERHSQFNPEKLKEELIQQTAFTIN-TKEAADIEACVIEVLSGNTCLLIEGNEEFFVVS 129 + S + + +E I+ TI+ E I+ + +VL G+T LLI+G + F++ Sbjct: 98 EYKNESSYVEGTVSKEYIKNKVLTISEVAEVHYIKDLIPKVLIGSTALLIDGLADVFILG 157 Query: 130 TGKFPLRSVEEPNTETLVRGPREGFIEDLESNLAMIRRHIKDLSFKIDKIRIGRRSSRKL 189 T K R+VEEP +E LVRGPR GF E L N +++RRH D S + K+ +G R+ ++L Sbjct: 158 TTKANKRNVEEPVSEALVRGPRVGFTEVLSDNTSLLRRHCADESLSLVKLPVGERAKKEL 217 Query: 190 AVLYISDIANPKLVQEVKRRVETIDVDDASDTGIIEQWIEESSWSPFPQIQASERSDKAI 249 V +I D+A+ +LV E+K R++ ID+D ++G IEQ IE++ SPFPQIQ +ER D+ I Sbjct: 218 VVAFIKDLASTELVDEIKNRIQKIDIDSVLESGYIEQLIEDNYLSPFPQIQNTERPDRVI 277 Query: 250 TALMSGRVVILLDGTPFALIAPMTFWMLAQSPEDYYEXXXXXXXXXXXXXXXXXXXXXXP 309 +ALM GRV ILLDGTPF LIAP+TF ML QSPEDYYE P Sbjct: 278 SALMEGRVAILLDGTPFVLIAPVTFSMLLQSPEDYYERWLPGTLLRLLRFMTAFVSLFAP 337 Query: 310 SLYVALISFHPGLIPPELVISIISSREGIPFPVFVEALIMEVSLEILREASLRLPKXXXX 369 +LY++ ISFHPGLIP +L ISII SREG+PFP +EALIMEV++E+LREA LRLPK Sbjct: 338 ALYISFISFHPGLIPTKLAISIIGSREGVPFPALIEALIMEVAIEVLREAGLRLPKPIGP 397 Query: 370 XXXXXXXXXXXXXXXEAAVVSSFLIIIVGLTAIASFASPQYSGSIGIRLLRFPIMIIAAI 429 EA +VS ++I+V +TAI+SF PQYS I +R+LRF M AAI Sbjct: 398 AMGIVGGLIIGQAAVEAGIVSPIMVIVVAVTAISSFTIPQYSVGITLRILRFVAMFCAAI 457 Query: 430 YGLYGVILAFIFLGAHMVQIKSFGVPYMSPLAPIRLRDQQDTLVRVPFKYQKKRPIMLKT 489 +GLYGVIL F+ L +H+V++KSFGVPY SP P R D +D +VR+P K ++RP ++ T Sbjct: 458 FGLYGVILFFLLLCSHLVKLKSFGVPYTSPAVPYRFSDWKDFMVRMPLKLMRRRPKIMHT 517 Query: 490 Q 490 + Sbjct: 518 K 518 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.322 0.139 0.393 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,491,919,272 Number of extensions: 92516880 Number of successful extensions: 249389 Number of sequences better than 1.0e-05: 1091 Number of HSP's gapped: 246295 Number of HSP's successfully gapped: 1094 Length of query: 500 Length of database: 3,060,696,203 Length adjustment: 140 Effective length of query: 360 Effective length of database: 1,741,754,103 Effective search space: 627031477080 Effective search space used: 627031477080 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 133 (55.8 bits)