BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0341.0001.p (276 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value C0ZIP8.1 C0ZIP8_BREBN Putative spore germination protein 147 9e-34 C2WE61.1 C2WE61_BACCE Spore germination protein 147 9e-34 B3K871.1 B3K871_9BACI Spore germination protein 141 6e-32 Q65IP9.1 Q65IP9_BACLD Spore germination protein 137 9e-31 C3BA78.1 C3BA78_BACMY Spore germination protein 137 1e-30 >C0ZIP8.1 C0ZIP8_BREBN Putative spore germination protein Length = 369 Score = 147 bits (371), Expect = 9e-34, Method: Compositional matrix adjust. Identities = 82/265 (30%), Positives = 124/265 (46%) Query: 1 VYAVAIVSLPRTIAEQTQTPDVWQGLLLASLFGVGVVFINVKLCQRFPGKTFYELNSVIT 60 + I++LPR E+ +TPDVW +++ L + I VKL QRFP KTF+E + I Sbjct: 22 ILGAGILTLPRISVEKAKTPDVWISVIIGGLLAMLAGIIIVKLSQRFPEKTFFEYSQEIA 81 Query: 61 GKFISLLINIAFMVYCVMICSLVCRMMAEFLKALALEKTPVSMIXXXXXXXXXXXTSGGL 120 G+++ ++++ + Y M SL R MAE LEKTP+ I GG+ Sbjct: 82 GEWVGRMLSLLIICYFCMTSSLQVRSMAEVTTFYLLEKTPMWAIVMVFLWVALYLVIGGI 141 Query: 121 HAMVRLIEXXXXXXXXXXXXXXXXXXXXXXXDHLRPVFHQGWGPIFKSLKVVPFATLGYE 180 + + R++E DHLRPV GW P+F +K A G+E Sbjct: 142 NCLARMLEIILPITVLFFLVVSLMSIKIFEIDHLRPVLGLGWKPVFDGIKPTALAYTGFE 201 Query: 181 SMLILTNFMVNPQKAWKAGAIGYSIAMALYVLMVTMVIGCMSVEEVSRLQWPVVSFAQQI 240 M+I+ +M P K KA +G S ++ Y++ V MVIG S++ V WP + + Sbjct: 202 IMMIIVVYMRQPNKGVKAVVVGISASLIFYLMTVVMVIGAFSIDGVVTRTWPTIDLIRSF 261 Query: 241 EFPGSXXXXXXXXXXXXWTIKIFMT 265 E G W ++IF T Sbjct: 262 EITGLLFERFESLLLVLWIMQIFST 286 >C2WE61.1 C2WE61_BACCE Spore germination protein Length = 368 Score = 147 bits (371), Expect = 9e-34, Method: Compositional matrix adjust. Identities = 91/265 (34%), Positives = 127/265 (47%) Query: 1 VYAVAIVSLPRTIAEQTQTPDVWQGLLLASLFGVGVVFINVKLCQRFPGKTFYELNSVIT 60 V AV I++LPRT AE+ +TPDVW +++ L + I VKL Q+FPGKTFY+ + I Sbjct: 22 VLAVGILTLPRTAAEKVKTPDVWITVIVGGLIAMVAGVIIVKLSQQFPGKTFYQYSQDIV 81 Query: 61 GKFISLLINIAFMVYCVMICSLVCRMMAEFLKALALEKTPVSMIXXXXXXXXXXXTSGGL 120 GK++ L+++ + Y VM+ S RM+ E LE TP I GG+ Sbjct: 82 GKWVGALLSLFIIGYFVMLSSFEVRMLGEITSFFLLEGTPGWAIIMPFMWVSIYLNLGGI 141 Query: 121 HAMVRLIEXXXXXXXXXXXXXXXXXXXXXXXDHLRPVFHQGWGPIFKSLKVVPFATLGYE 180 +++ RL E D+LRPV G P+ K +K A G E Sbjct: 142 NSIARLFEIIFPITVFIFLMTSFMSIGMFELDNLRPVLGLGVLPVLKGIKTTTLAYSGVE 201 Query: 181 SMLILTNFMVNPQKAWKAGAIGYSIAMALYVLMVTMVIGCMSVEEVSRLQWPVVSFAQQI 240 ML+L FM P +A K IG +I + YV+ V MVIG SV+ V WP + + Sbjct: 202 IMLLLLAFMKQPLQAVKVVLIGTAIPLIFYVITVVMVIGAFSVDGVVMRTWPSIDLMRSF 261 Query: 241 EFPGSXXXXXXXXXXXXWTIKIFMT 265 E PG W ++IF T Sbjct: 262 EIPGLLFERFESLLLVIWIMQIFAT 286 >B3K871.1 B3K871_9BACI Spore germination protein Length = 369 Score = 141 bits (355), Expect = 6e-32, Method: Compositional matrix adjust. Identities = 80/261 (30%), Positives = 128/261 (49%) Query: 5 AIVSLPRTIAEQTQTPDVWQGLLLASLFGVGVVFINVKLCQRFPGKTFYELNSVITGKFI 64 I++LPRT E +TPDVW +L++ + + FI V LC+RF GKT+++ ITGK++ Sbjct: 29 GILTLPRTTVEAAKTPDVWISILISGMIIMLAGFIVVTLCRRFQGKTYFQFVPEITGKWV 88 Query: 65 SLLINIAFMVYCVMICSLVCRMMAEFLKALALEKTPVSMIXXXXXXXXXXXTSGGLHAMV 124 + L+++ + Y + I + R+MAE LE TPV +I T+GG++ + Sbjct: 89 AFLLSLLVIAYLITISAFEIRVMAEVTGLYLLEGTPVWIIVMTFMWIGLYLTTGGINCIA 148 Query: 125 RLIEXXXXXXXXXXXXXXXXXXXXXXXDHLRPVFHQGWGPIFKSLKVVPFATLGYESMLI 184 R+ E +LRPV G P+ K +K + GYE M + Sbjct: 149 RMYEIILPITLLFFIIAIMLSSSIFDVKNLRPVLGSGIMPVLKGIKPSLLSFTGYEIMFV 208 Query: 185 LTNFMVNPQKAWKAGAIGYSIAMALYVLMVTMVIGCMSVEEVSRLQWPVVSFAQQIEFPG 244 FM +P+K KA +G ++ M +Y++ V MVIG +S+ V + WP + + E G Sbjct: 209 AMAFMKSPKKGTKAMLVGITVPMVIYLITVIMVIGGLSIHGVQKRTWPTLDLMRSFEMQG 268 Query: 245 SXXXXXXXXXXXXWTIKIFMT 265 W ++IF T Sbjct: 269 LIFERFESLLLVIWIMQIFST 289 >Q65IP9.1 Q65IP9_BACLD Spore germination protein Length = 366 Score = 137 bits (345), Expect = 9e-31, Method: Compositional matrix adjust. Identities = 83/265 (31%), Positives = 121/265 (45%) Query: 3 AVAIVSLPRTIAEQTQTPDVWQGLLLASLFGVGVVFINVKLCQRFPGKTFYELNSVITGK 62 I++LPRT ++ TPDVW L+L L + I VKL ++P KTFYE + I GK Sbjct: 24 GTGILTLPRTSVDEVGTPDVWITLVLGGLVALTAAVIMVKLNHQYPDKTFYEYSQDIIGK 83 Query: 63 FISLLINIAFMVYCVMICSLVCRMMAEFLKALALEKTPVSMIXXXXXXXXXXXTSGGLHA 122 + LI++ F Y + R M E ++ LE TP + GGL + Sbjct: 84 WGGSLISLFFACYLLAHSGFQVRSMLEIIRFFLLEGTPRWAVAMVFLWVGLYLIVGGLSS 143 Query: 123 MVRLIEXXXXXXXXXXXXXXXXXXXXXXXDHLRPVFHQGWGPIFKSLKVVPFATLGYESM 182 + RL + D+LRPV +G GP+ K +KV + G E + Sbjct: 144 IARLFQIVLPITFILFLLVVFMSIGIFDVDNLRPVLGEGIGPVLKGIKVTALSFSGIEII 203 Query: 183 LILTNFMVNPQKAWKAGAIGYSIAMALYVLMVTMVIGCMSVEEVSRLQWPVVSFAQQIEF 242 LIL FM P+KA IG +I + Y++ V MVIG +S++ V WP + + E Sbjct: 204 LILMPFMNQPRKAIHTIMIGIAIPLLFYMITVVMVIGALSIDGVVSRTWPTIDLMRSFEI 263 Query: 243 PGSXXXXXXXXXXXXWTIKIFMTTA 267 PG W +++F T A Sbjct: 264 PGLIFERFESLLLVIWIMQLFCTFA 288 >C3BA78.1 C3BA78_BACMY Spore germination protein Length = 339 Score = 137 bits (344), Expect = 1e-30, Method: Compositional matrix adjust. Identities = 84/257 (32%), Positives = 118/257 (45%) Query: 9 LPRTIAEQTQTPDVWQGLLLASLFGVGVVFINVKLCQRFPGKTFYELNSVITGKFISLLI 68 +PRT AE+ TPDVW ++L L + I VKL Q+FPGKTFY+ N I GK++ ++ Sbjct: 1 MPRTAAEKVNTPDVWITVILGGLIAMVAGIIMVKLSQQFPGKTFYQYNQEIVGKWVGAIL 60 Query: 69 NIAFMVYCVMICSLVCRMMAEFLKALALEKTPVSMIXXXXXXXXXXXTSGGLHAMVRLIE 128 ++ + Y M+ S + + LE TP I GG++++ RL E Sbjct: 61 SLFIVGYFFMLSSFEVKTLEGITSFFLLEGTPGWAIIMPFMWVSLYLNLGGINSIARLFE 120 Query: 129 XXXXXXXXXXXXXXXXXXXXXXXDHLRPVFHQGWGPIFKSLKVVPFATLGYESMLILTNF 188 D+LRPV G P+ K LK A G E ML+L F Sbjct: 121 IIFPITVFIFLFTSFMSIGIFEIDNLRPVLGLGIIPVLKGLKTTTLAYSGAEIMLLLLAF 180 Query: 189 MVNPQKAWKAGAIGYSIAMALYVLMVTMVIGCMSVEEVSRLQWPVVSFAQQIEFPGSXXX 248 M P++A K IG +I + YV+ V MVIG SV+ V WP + + E PG Sbjct: 181 MKQPRQAVKVVLIGTAIPLIFYVITVVMVIGTFSVDGVLMRTWPTIDLMRSFELPGLIFE 240 Query: 249 XXXXXXXXXWTIKIFMT 265 W ++IF T Sbjct: 241 RFESLLLVIWIMQIFAT 257 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.330 0.140 0.434 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,151,793,556 Number of extensions: 35251536 Number of successful extensions: 122391 Number of sequences better than 1.0e-05: 294 Number of HSP's gapped: 122203 Number of HSP's successfully gapped: 294 Length of query: 276 Length of database: 3,060,696,203 Length adjustment: 134 Effective length of query: 142 Effective length of database: 1,798,280,193 Effective search space: 255355787406 Effective search space used: 255355787406 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.9 bits) S2: 130 (54.7 bits)