BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0340.0044.p (328 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value C2XEJ1.1 C2XEJ1_BACCE Nonribosomal peptide synthetase 65 1e-08 C3I384.1 C3I384_BACTU Nonribosomal peptide synthetase 65 1e-08 C3FUA1.1 C3FUA1_BACTB Nonribosomal peptide synthetase 65 1e-08 C3DAQ0.1 C3DAQ0_BACTU Nonribosomal peptide synthetase 65 1e-08 B5UXK3.1 B5UXK3_BACCE Bacitracin synthetase 3 (BA3) EC=5.1.1.11 ... 64 2e-08 >C2XEJ1.1 C2XEJ1_BACCE Nonribosomal peptide synthetase Length = 883 Score = 64.7 bits (156), Expect = 1e-08, Method: Compositional matrix adjust. Identities = 71/335 (21%), Positives = 139/335 (41%), Gaps = 41/335 (12%) Query: 1 MLNIVPVKSTPYPTLRSCIDDCIHSVAEWLGKDSKCMYGNAWQVSFEEQQFPDQPLIERL 60 + ++ P KS+ ++CIDD I ++A W + MY W F P+ Sbjct: 9 LYDVEPCKSSE----QNCIDDVIATLANWFKRKHYLMYARLWDFGF-------SPVDNGN 57 Query: 61 ALPRISTEA------LSAYHGLVFSYIERKEPEMDKTFMLDMLESRLSSGMPVLVGFDSY 114 + TE+ L YHG+ + + D+ F +++ +L SG PV+V ++Y Sbjct: 58 LGDGLYTESGYFVSDLEKYHGIKSTIFDTDSS--DEGF--EIILEQLYSGFPVVVTMNAY 113 Query: 115 DCPWCLAFRKLHTSHACLAVGLDRSSHLIYLTDAYYGKTLEPVDFDVFQKACHFYALFEL 174 PW + K H H+ + VG + +TD Y+ + ++F K + ++ Sbjct: 114 WLPWDENYLKNHIVHSFVIVGYEPEEDCFIITDPYFMVKNAQLSTELFHKGYQKVSTLKV 173 Query: 175 CDSSRSYTDWQATLQGMLSSPINQVQPDQ-VAAHLRAYADAYLNTGITADCPAEYSSSFM 233 + TD Q+ + LS+ I ++ ++ + + + +A T + + + Sbjct: 174 TED--EITDIQS-ITTDLSNWIKHIKDEKHIFSQMHLFA-----TKLISRFDLNKETDGY 225 Query: 234 LAANTLPIH---------RIRFSLFLRLLNREAGLTALSQAAEGYQLSGEQWNLINQFMI 284 L N +P+ R++FS L L E + AE + + W +I +I Sbjct: 226 LDFNDVPLFSHLGQVVDGRVKFSKMLSFLG-ERYHSDFQTIAESMKTAAANWGVIRGSLI 284 Query: 285 KVMCSGNKLSQR-RKIHKKMHEMISLEEQVLEELT 318 K+ + + + I K+H E+ + E++T Sbjct: 285 KMHLTKSVTKEGIVNISDKIHHAAEYEQNLAEQIT 319 >C3I384.1 C3I384_BACTU Nonribosomal peptide synthetase Length = 884 Score = 64.7 bits (156), Expect = 1e-08, Method: Compositional matrix adjust. Identities = 70/334 (20%), Positives = 140/334 (41%), Gaps = 41/334 (12%) Query: 1 MLNIVPVKSTPYPTLRSCIDDCIHSVAEWLGKDSKCMYGNAWQVSFEEQQFPDQPLIERL 60 + ++ P KS+ ++CIDD I ++A W + MY W F P+ + Sbjct: 9 LYDVEPCKSSE----QNCIDDVIATLANWFKRKHYLMYARLWDFGF-------SPVDKGN 57 Query: 61 ALPRISTEA------LSAYHGLVFSYIERKEPEMDKTFMLDMLESRLSSGMPVLVGFDSY 114 + TE+ L YHG+ + D+ F +++ +L SG PV+V ++Y Sbjct: 58 LGDGLYTESGYFVSDLEKYHGIKSTIFNTDSS--DEGF--EIILEQLYSGFPVVVTMNAY 113 Query: 115 DCPWCLAFRKLHTSHACLAVGLDRSSHLIYLTDAYYGKTLEPVDFDVFQKACHFYALFEL 174 PW + K H H+ + VG + + +TD Y+ + ++F K + ++ Sbjct: 114 WLPWDENYLKNHIVHSFVIVGYEPEEDCLIITDPYFMVKNAQLSTELFHKGYQKVSTLKV 173 Query: 175 CDSSRSYTDWQATLQGMLSSPINQVQPDQ-VAAHLRAYADAYLNTGITADCPAEYSSSFM 233 + TD Q+ + LS+ I ++ ++ + + + +A T + + + Sbjct: 174 TED--EITDIQS-ITTDLSNWIKHIKDEKHIFSQMHLFA-----TQLISKFDLNKETDGY 225 Query: 234 LAANTLPIH---------RIRFSLFLRLLNREAGLTALSQAAEGYQLSGEQWNLINQFMI 284 L N +P+ R++FS L L E + AE + + W +I +I Sbjct: 226 LDFNDVPLFSHLGQVVDGRVKFSKMLSFLG-ERYHSDFQTIAESMKTAAANWGVIRGSLI 284 Query: 285 KVMCSGNKLSQR-RKIHKKMHEMISLEEQVLEEL 317 K+ + + + + I K+H E+ + E++ Sbjct: 285 KMHLTKSVIKEGIVNISDKIHHAAEYEQNLAEQI 318 >C3FUA1.1 C3FUA1_BACTB Nonribosomal peptide synthetase Length = 883 Score = 64.7 bits (156), Expect = 1e-08, Method: Compositional matrix adjust. Identities = 71/334 (21%), Positives = 138/334 (41%), Gaps = 41/334 (12%) Query: 1 MLNIVPVKSTPYPTLRSCIDDCIHSVAEWLGKDSKCMYGNAWQVSFEEQQFPDQPLIERL 60 + ++ P KS+ ++CIDD I ++A W + MY W SF P+ Sbjct: 9 LYDVEPCKSSE----QNCIDDVIATLANWFKRKHYLMYARLWDFSF-------SPVDNGN 57 Query: 61 ALPRISTEA------LSAYHGLVFSYIERKEPEMDKTFMLDMLESRLSSGMPVLVGFDSY 114 + TE+ L YHG+ + + D+ F +++ +L SG PV+V ++Y Sbjct: 58 LGDGLYTESGYFVSDLEKYHGIKSTIFDTDSS--DEGF--EIILEQLYSGFPVVVTMNAY 113 Query: 115 DCPWCLAFRKLHTSHACLAVGLDRSSHLIYLTDAYYGKTLEPVDFDVFQKACHFYALFEL 174 PW + K H H+ + VG + + +TD Y+ + ++F K + ++ Sbjct: 114 WLPWDENYLKNHIVHSFVVVGYESEEDCLIITDPYFMVKNAQLSIELFHKGYQKVSTLKV 173 Query: 175 CDSSRSYTDWQATLQGMLSSPINQVQPDQ-VAAHLRAYADAYLNTGITADCPAEYSSSFM 233 + TD Q+ + LS+ I ++ + + + + +A T + + + Sbjct: 174 TED--EITDIQS-ITTDLSNWIKHIKDKKHIFSQMHLFA-----TQLISRFDLNKETDGY 225 Query: 234 LAANTLPIH---------RIRFSLFLRLLNREAGLTALSQAAEGYQLSGEQWNLINQFMI 284 L N +P+ R++FS L L E AE + + W +I +I Sbjct: 226 LDFNDVPLFSHLGQVVDGRVKFSKMLSFLG-EHYHPDFQTIAESMKTAAANWGVIRGSLI 284 Query: 285 KVMCSGNKLSQR-RKIHKKMHEMISLEEQVLEEL 317 K+ + + + I K+H E+ + E++ Sbjct: 285 KMHLTKSVTKEGIVNISDKIHHAAEYEQNLAEQI 318 >C3DAQ0.1 C3DAQ0_BACTU Nonribosomal peptide synthetase Length = 883 Score = 64.7 bits (156), Expect = 1e-08, Method: Compositional matrix adjust. Identities = 71/334 (21%), Positives = 138/334 (41%), Gaps = 41/334 (12%) Query: 1 MLNIVPVKSTPYPTLRSCIDDCIHSVAEWLGKDSKCMYGNAWQVSFEEQQFPDQPLIERL 60 + ++ P KS+ ++CIDD I ++A W + MY W SF P+ Sbjct: 9 LYDVEPCKSSE----QNCIDDVIATLANWFKRKHYLMYARLWDFSF-------SPVDNGN 57 Query: 61 ALPRISTEA------LSAYHGLVFSYIERKEPEMDKTFMLDMLESRLSSGMPVLVGFDSY 114 + TE+ L YHG+ + + D+ F +++ +L SG PV+V ++Y Sbjct: 58 LGDGLYTESGYFVSDLEKYHGIKSTIFDTDSS--DEGF--EIILEQLYSGFPVVVTMNAY 113 Query: 115 DCPWCLAFRKLHTSHACLAVGLDRSSHLIYLTDAYYGKTLEPVDFDVFQKACHFYALFEL 174 PW + K H H+ + VG + + +TD Y+ + ++F K + ++ Sbjct: 114 WLPWDENYLKNHIVHSFVVVGYESEEDCLIITDPYFMVKNAQLSIELFHKGYQKVSTLKV 173 Query: 175 CDSSRSYTDWQATLQGMLSSPINQVQPDQ-VAAHLRAYADAYLNTGITADCPAEYSSSFM 233 + TD Q+ + LS+ I ++ + + + + +A T + + + Sbjct: 174 TED--EITDIQS-ITTDLSNWIKHIKDKKHIFSQMHLFA-----TQLISRFDLNKETDGY 225 Query: 234 LAANTLPIH---------RIRFSLFLRLLNREAGLTALSQAAEGYQLSGEQWNLINQFMI 284 L N +P+ R++FS L L E AE + + W +I +I Sbjct: 226 LDFNDVPLFSHLGQVVDGRVKFSKMLSFLG-EHYHPDFQTIAESMKTAAANWGVIRGSLI 284 Query: 285 KVMCSGNKLSQR-RKIHKKMHEMISLEEQVLEEL 317 K+ + + + I K+H E+ + E++ Sbjct: 285 KMHLTKSVTKEGIVNISDKIHHAAEYEQNLAEQI 318 >B5UXK3.1 B5UXK3_BACCE Bacitracin synthetase 3 (BA3) EC=5.1.1.11 EC=5.1.1.13 Length = 883 Score = 63.5 bits (153), Expect = 2e-08, Method: Compositional matrix adjust. Identities = 70/334 (20%), Positives = 139/334 (41%), Gaps = 41/334 (12%) Query: 1 MLNIVPVKSTPYPTLRSCIDDCIHSVAEWLGKDSKCMYGNAWQVSFEEQQFPDQPLIERL 60 + ++ P KS+ ++CIDD I ++A W + MY W F P+ Sbjct: 9 LYDVEPCKSSE----QNCIDDVIATLANWFKRKHYLMYARLWDFGF-------SPVDNGN 57 Query: 61 ALPRISTEA------LSAYHGLVFSYIERKEPEMDKTFMLDMLESRLSSGMPVLVGFDSY 114 + TE+ L YHG+ + + D+ F +++ +L SG PV+V ++Y Sbjct: 58 LGDGLYTESGYFVSDLEKYHGIKSTIFDTDSS--DEGF--EIILEQLYSGFPVVVTMNAY 113 Query: 115 DCPWCLAFRKLHTSHACLAVGLDRSSHLIYLTDAYYGKTLEPVDFDVFQKACHFYALFEL 174 PW + K H H+ + VG + + +TD Y+ + ++F K + ++ Sbjct: 114 WLPWDENYLKNHIVHSFVIVGYEPEEDCLIITDPYFMVKNAQLSTELFHKGYQKVSTLKV 173 Query: 175 CDSSRSYTDWQATLQGMLSSPINQVQPDQ-VAAHLRAYADAYLNTGITADCPAEYSSSFM 233 + TD Q+ + LS+ I ++ ++ + + + +A T + + + Sbjct: 174 TED--EITDIQS-ITTDLSNWIKHIKDEKHIFSQMHLFA-----TKLISRFDLNKETDGY 225 Query: 234 LAANTLPIH---------RIRFSLFLRLLNREAGLTALSQAAEGYQLSGEQWNLINQFMI 284 L N +P+ R++FS L L E + AE + + W +I +I Sbjct: 226 LDFNDVPLFSHLGQVVDGRVKFSKMLSFLG-ERYHSDFQTIAESMKTAAANWGVIRGSLI 284 Query: 285 KVMCSGNKLSQR-RKIHKKMHEMISLEEQVLEEL 317 K+ + + + I K+H E+ + E++ Sbjct: 285 KMHLTKSVTKEGIVNISDKIHHAAEYEQNLAEQI 318 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.322 0.134 0.410 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,908,615,093 Number of extensions: 75328834 Number of successful extensions: 177901 Number of sequences better than 1.0e-05: 10 Number of HSP's gapped: 177893 Number of HSP's successfully gapped: 10 Length of query: 328 Length of database: 3,060,696,203 Length adjustment: 136 Effective length of query: 192 Effective length of database: 1,779,438,163 Effective search space: 341652127296 Effective search space used: 341652127296 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 131 (55.1 bits)