BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0340.0043.p (389 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1DB13.1 B1DB13_9BACL Germination protein, Ger(X)C family 400 e-109 B3K872.1 B3K872_9BACI Germination protein, Ger(X)C family 344 9e-93 C3ASS8.1 C3ASS8_BACMY Spore germination protein BC 304 9e-81 C2WE60.1 C2WE60_BACCE Spore germination protein BC 303 3e-80 C0ZIP7.1 C0ZIP7_BREBN Putative spore germination protein 302 3e-80 >B1DB13.1 B1DB13_9BACL Germination protein, Ger(X)C family Length = 395 Score = 400 bits (1027), Expect = e-109, Method: Compositional matrix adjust. Identities = 183/389 (47%), Positives = 274/389 (70%), Gaps = 4/389 (1%) Query: 1 LAAALLLTETGCWSSNEIEDLSMYTALTIDAGQPTQTEQTFEKEGGSYLKDNKITVTIEI 60 LAAA+L T++GCWSSNEIEDL++Y + +D+G+ E+ FE+EGGSY K NKIT T++I Sbjct: 11 LAAAVLFTQSGCWSSNEIEDLAVYAGMALDSGKENPEEKAFEQEGGSYSKQNKITATVQI 70 Query: 61 VPKQSYGNSNKTPDTGAKAPNYVNISETGDSVLEILRQFAIRLDRPVIGHHLKVIVISRQ 120 VP ++ +K G +P Y NI+ TGDSVLEI RQF++RL++P+IGHHLK+IVIS Q Sbjct: 71 VPLKA---GSKDEVRGKTSP-YTNITGTGDSVLEIFRQFSLRLNKPIIGHHLKIIVISSQ 126 Query: 121 LLQEQRIQDLMDFVLRDNDIRPSCLILMSEGRAADTLKTKFGGEIPAFHLRGMTRNRFRN 180 LLQE+ I+ + +F+LRDNDIRPS I +S G+A D L K E+P+F + + N+FR Sbjct: 127 LLQERNIKQVTNFMLRDNDIRPSVKIFISSGKALDALTLKTPDEVPSFFINDVVENQFRT 186 Query: 181 SRIMKGIITSELDALMHAKKSFLLQNIVEANQEVEFSGGGVIKGDTGRWIGNLEQQDIES 240 S+IM ++ ++LDA+MH+K+SF+LQNI++A + +EFSG G+IKGDTGRWIGNL Q+D+E Sbjct: 187 SKIMDAVLLTDLDAMMHSKQSFILQNIIQAKKHIEFSGAGIIKGDTGRWIGNLTQEDVEC 246 Query: 241 IAWIKGDVKGGTLKTYNPDHQTVTYEXXXXXXXXXXXVNKDHEPEFHVSIESEGRLIEKW 300 I W+ GT+KTY+ +TYE ++D++ F V +E++GR+ E W Sbjct: 247 IDWLTNQGDSGTIKTYDEQGNPITYEMKAMKTRIRVETDQDNQFTFQVRVETKGRISEDW 306 Query: 301 KDPGLPSKKEYLEKMQGIFKERLTEMIQSLMHKMQSEFKVEVAGFGDRLSVEEPQEWKKL 360 G P+ + Y ++++ +F+ +LT MI+ L+ MQ+ +KV+VA F + +++P WK++ Sbjct: 307 DVEGNPALRSYQKEIEKLFESKLTSMIEHLLSAMQTRYKVDVAKFCQSVRIQKPGLWKEV 366 Query: 361 KDHWDETFSRTPVTFDIKLTITDYGSFTE 389 K +WD+TFS + + DI L +TD+GS T+ Sbjct: 367 KSNWDDTFSASKINLDIDLQVTDFGSTTK 395 >B3K872.1 B3K872_9BACI Germination protein, Ger(X)C family Length = 401 Score = 344 bits (882), Expect = 9e-93, Method: Compositional matrix adjust. Identities = 169/390 (43%), Positives = 257/390 (65%), Gaps = 3/390 (0%) Query: 2 AAALLLTETGCWSSNEIEDLSMYTALTIDAGQPTQTEQTFEKEGGSYLKDNKITVTIEIV 61 A+ LLL TGCWSSNEIED+S+Y L +DA ++ E+ ++G SY K +T T++IV Sbjct: 13 ASVLLLLTTGCWSSNEIEDVSVYVGLGLDAANESRFEREINQQGASYPKRKVLTATVQIV 72 Query: 62 PK-QSYGNSNKTPDTGAKAPNYVNISETGDSVLEILRQFAIRLDRPVIGHHLKVIVISRQ 120 P S + + Y+N TGDS+++I RQFA+R DRP+IGHHLKVIV+S + Sbjct: 73 PPITSKSEKQGGSSSSPPSKAYLNEQLTGDSLIQIFRQFALRRDRPLIGHHLKVIVVSSE 132 Query: 121 LLQEQRIQDLMDFVLRDNDIRPSCLILMSEGRAADTLKTKFGGEIPAFHLRGMTRNRFRN 180 L + ++ ++DF+LRDNDIRPSC++++S A + L + GEIPAF+L G+ NR+R+ Sbjct: 133 LAKRYSLEQILDFILRDNDIRPSCMVVVSHRSALEALSSTEPGEIPAFYLMGLVDNRYRS 192 Query: 181 SRIMKGIITSELDALMHAKKSFLLQNIVEANQEVEFSGGGVIKGDTGRWIGNLEQQDIES 240 ++I+ + +LD+ M + +SFLLQN+V A E +FSG V KG+T ++IG L Q D+E Sbjct: 193 NKILPPMSLIKLDSTMQSGESFLLQNVVTAQSEHKFSGAAVFKGNTKKFIGELSQYDLEG 252 Query: 241 IAWIKGDVKGGTLKTY-NPDHQTVTYEXXXXXXXXXXXVNKDHEPEFHVSIESEGRLIEK 299 ++WIK DVKGG LKTY + TV YE + D E FH+ + SEGRLIE Sbjct: 253 LSWIKEDVKGGALKTYAHKTGHTVIYEPKNSHSTIIPKIQGD-EISFHIKVRSEGRLIED 311 Query: 300 WKDPGLPSKKEYLEKMQGIFKERLTEMIQSLMHKMQSEFKVEVAGFGDRLSVEEPQEWKK 359 W P +P+ ++Y+ +++ +F+E + I+ ++ KMQ + V+V GF D++ ++ P+ WKK Sbjct: 312 WSFPEIPTTEQYMRELERLFEEEARKQIEQVLDKMQHVYHVDVGGFADQIRIKYPKLWKK 371 Query: 360 LKDHWDETFSRTPVTFDIKLTITDYGSFTE 389 ++ WDETFS P+T+++ + ITD+GS T+ Sbjct: 372 VEKDWDETFSHIPITYEVDMKITDFGSSTD 401 >C3ASS8.1 C3ASS8_BACMY Spore germination protein BC Length = 382 Score = 304 bits (779), Expect = 9e-81, Method: Compositional matrix adjust. Identities = 155/384 (40%), Positives = 239/384 (62%), Gaps = 7/384 (1%) Query: 5 LLLTETGCWSSNEIEDLSMYTALTIDAGQPTQTEQTFEKEGGSYLKDNKITVTIEIV-PK 63 +LL TGCWSS EIE+ L D G+ T+ E+ E+ GG Y K N IT T + V P+ Sbjct: 1 MLLALTGCWSSREIEEQGFAVGLAFDKGKETKVEKEIEEHGGGYRKRNLITSTYQFVKPE 60 Query: 64 QSYGNSNKTPDTGAKAPNYVNISETGDSVLEILRQFAIRLDRPVIGHHLKVIVISRQLLQ 123 S A Y NISETGDS+ + +R+ A+R RP+I HH KVIV+S L + Sbjct: 61 LSGKGKGGGGQQKA----YTNISETGDSLHQSIREVALRRQRPIIFHHTKVIVVSESLAR 116 Query: 124 EQRIQDLMDFVLRDNDIRPSCLILMSEGRAADTLKTKFGGEIPAFHLRGMTRNRFRNSRI 183 ++ L+D +RDN++RPSCL+L+S+GRA++TLK++ GEIPAF L G+ N++R+SRI Sbjct: 117 MYSLKQLLDIYIRDNEMRPSCLVLISKGRASETLKSREAGEIPAFLLIGIGDNQYRSSRI 176 Query: 184 MKGIITSELDALMHAKKSFLLQNIVEANQEVEFSGGGVIKGDTGRWIGNLEQQDIESIAW 243 + + ++L +H+ SFLL N++ N EV+F+G VIKG T + G L + ++E + W Sbjct: 177 LPPVPLAKLPGKIHSGASFLLPNVISINGEVKFAGAAVIKGKTKKLRGFLNENELEGLTW 236 Query: 244 IKGDVKGGTLKTYNPD-HQTVTYEXXXXXXXXXXXVNKDHEPEFHVSIESEGRLIEKWKD 302 I G K G +K+++ + + YE V K + F V+I+SEGRL E W Sbjct: 237 ITGKGKSGLVKSFDEKTGKPILYEVKSMKSKIQPHV-KGNNISFDVNIQSEGRLSENWVA 295 Query: 303 PGLPSKKEYLEKMQGIFKERLTEMIQSLMHKMQSEFKVEVAGFGDRLSVEEPQEWKKLKD 362 G P + ++L ++ ++ + +++ + K+Q E++V+VAGFG+RL +E P+ WKK++ Sbjct: 296 SGEPFENKFLRSVEKSAEQEVNRLVKRTLKKIQKEYQVDVAGFGNRLKIEYPKVWKKVEK 355 Query: 363 HWDETFSRTPVTFDIKLTITDYGS 386 WD+TFS+ P+ + +KLTI DYG+ Sbjct: 356 DWDKTFSKVPIKYSVKLTIEDYGT 379 >C2WE60.1 C2WE60_BACCE Spore germination protein BC Length = 382 Score = 303 bits (775), Expect = 3e-80, Method: Compositional matrix adjust. Identities = 154/384 (40%), Positives = 240/384 (62%), Gaps = 7/384 (1%) Query: 5 LLLTETGCWSSNEIEDLSMYTALTIDAGQPTQTEQTFEKEGGSYLKDNKITVTIEIV-PK 63 +LL TGCWSS+EIE+ L D G+ T E+ E+ GG Y K N IT T + V P+ Sbjct: 1 MLLALTGCWSSHEIEEQGFAVGLAFDKGKETDVERDIEEHGGGYRKRNLITSTYQFVKPE 60 Query: 64 QSYGNSNKTPDTGAKAPNYVNISETGDSVLEILRQFAIRLDRPVIGHHLKVIVISRQLLQ 123 S A Y N+SETGDS+ + +R+ A+R RP+I HH KVIV+S L + Sbjct: 61 LSGKGKGGGGQQKA----YTNVSETGDSLHQSIREVALRRPRPIIFHHTKVIVVSANLAR 116 Query: 124 EQRIQDLMDFVLRDNDIRPSCLILMSEGRAADTLKTKFGGEIPAFHLRGMTRNRFRNSRI 183 ++ L+D +RDN+IRPSCL+L+S+GRA++TLK++ GEIPAF L G+ N++R+SRI Sbjct: 117 TYSLKQLLDIYIRDNEIRPSCLVLISKGRASETLKSREAGEIPAFLLLGIGDNQYRSSRI 176 Query: 184 MKGIITSELDALMHAKKSFLLQNIVEANQEVEFSGGGVIKGDTGRWIGNLEQQDIESIAW 243 + + ++L + + SFLL N++ N EV+F+G VIKG T + G L + ++E + W Sbjct: 177 LPPVPLAKLPGKIESGSSFLLPNVIPVNGEVKFAGAAVIKGKTKKLRGFLNENELEGLTW 236 Query: 244 IKGDVKGGTLKTYN-PDHQTVTYEXXXXXXXXXXXVNKDHEPEFHVSIESEGRLIEKWKD 302 I G K G +K+++ + + YE V K + F V+I+SEGRL E W Sbjct: 237 ITGKGKSGLVKSFDEKTGKPILYEVKSMKSKIQPHV-KGNNISFDVNIQSEGRLSENWVA 295 Query: 303 PGLPSKKEYLEKMQGIFKERLTEMIQSLMHKMQSEFKVEVAGFGDRLSVEEPQEWKKLKD 362 G P + ++L+ ++ K+ + +++ + K+Q E++V+VAGFG+RL +E P+ WKK++ Sbjct: 296 SGEPFENKFLKSVEKSTKQEVNRLVKHTLKKIQKEYQVDVAGFGNRLKIEYPKVWKKVEK 355 Query: 363 HWDETFSRTPVTFDIKLTITDYGS 386 WD+TFS+ P+ +++K+TI DYG+ Sbjct: 356 DWDKTFSKIPIKYNVKITIQDYGT 379 >C0ZIP7.1 C0ZIP7_BREBN Putative spore germination protein Length = 404 Score = 302 bits (774), Expect = 3e-80, Method: Compositional matrix adjust. Identities = 151/379 (39%), Positives = 239/379 (63%), Gaps = 6/379 (1%) Query: 10 TGCWSSNEIEDLSMYTALTIDAGQPTQTEQTFEKEGGSYLKDNKITVTIEIV-PKQSYGN 68 TGCWSSN+I+ + M L +DA + E+ EK+G S + IT+T + + PK + Sbjct: 25 TGCWSSNDIDKMGMGIGLALDASNKSNVEKKLEKQGKSPPHVDTITMTYQFINPKAT--- 81 Query: 69 SNKTPDTGAKAPNYVNISETGDSVLEILRQFAIRLDRPVIGHHLKVIVISRQLLQEQRIQ 128 S ++ G + Y+N S+TG+S+L I+R+F++ D P+ HLKVIVIS LL R++ Sbjct: 82 SKESSSAGPQQKAYLNASQTGESILPIIREFSLSHDTPLFSPHLKVIVISEALLHTHRLE 141 Query: 129 DLMDFVLRDNDIRPSCLILMSEGRAADTLKTKFGGEIPAFHLRGMTRNRFRNSRIMKGII 188 L+D LRDN+IRPSCL+L+S G A D L+TK GE+PA +L G+ NR R ++I+ + Sbjct: 142 SLLDLFLRDNEIRPSCLLLVSNGNARDVLETKKPGELPALNLLGIVDNRNRTAKILPPVS 201 Query: 189 TSELDALMHAKKSFLLQNIVEANQEVEFSGGGVIKGDTGRWIGNLEQQDIESIAWIKGDV 248 ++L A ++++ SFLLQN+ +N+EV+F G VI G T +W G L + D+E I WI G Sbjct: 202 LAKLTAKLNSESSFLLQNVAASNKEVKFEGAAVINGKTKKWAGFLNEMDLEGITWITGKG 261 Query: 249 KGGTLKTYNPDH-QTVTYEXXXXXXXXXXXVNKDHEPEFHVSIESEGRLIEKWKDPGLPS 307 KGG +++ + + Q + YE V+ + + F V I S+GRL E W P Sbjct: 262 KGGLVRSLDKETGQLIIYEIETMESKITPHVDGN-KISFDVHINSKGRLSENWVLNANPF 320 Query: 308 KKEYLEKMQGIFKERLTEMIQSLMHKMQSEFKVEVAGFGDRLSVEEPQEWKKLKDHWDET 367 + E+L++++ + + ++++++ KMQ+ F +VAGFG+RL +E P+ W+K+K WD+T Sbjct: 321 ENEFLKRVEKAAELEVRKLVKNVTAKMQNHFHADVAGFGERLRIEYPKTWEKVKKDWDQT 380 Query: 368 FSRTPVTFDIKLTITDYGS 386 FS P+T+ +KLT TD+G+ Sbjct: 381 FSTVPITYFVKLTTTDFGA 399 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.316 0.134 0.387 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,299,917,608 Number of extensions: 93360600 Number of successful extensions: 232914 Number of sequences better than 1.0e-05: 272 Number of HSP's gapped: 232446 Number of HSP's successfully gapped: 273 Length of query: 389 Length of database: 3,060,696,203 Length adjustment: 138 Effective length of query: 251 Effective length of database: 1,760,596,133 Effective search space: 441909629383 Effective search space used: 441909629383 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 132 (55.5 bits)