BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0340.0042.p (367 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1DB12.1 B1DB12_9BACL Spore germination protein 421 e-116 B7HWN7.1 B7HWN7_BACC7 Spore germination protein A2 349 2e-94 C3C492.1 C3C492_BACTU Spore germination protein 349 2e-94 Q7BYC0.1 Q7BYC0_BACCR Spore germination protein BB 349 2e-94 Q9K337.1 Q9K337_BACCE YndE protein 349 2e-94 >B1DB12.1 B1DB12_9BACL Spore germination protein Length = 374 Score = 421 bits (1083), Expect = e-116, Method: Compositional matrix adjust. Identities = 211/365 (57%), Positives = 263/365 (72%) Query: 2 FVRSDQKITSTQAAIFLTNSVLGAGILTLPRSVTESAKTPDAWLSXXXXXXXXXXXXXXX 61 F+ D KITS+QAA+ L N++LGAGILTLPRSV+E+ KTPD+W+S Sbjct: 7 FLAQDDKITSSQAALVLNNTMLGAGILTLPRSVSETVKTPDSWISVLLGGLIVMLVVVLM 66 Query: 62 XKLSQQFPGKTVYQYSRQIAGSIPGSFLSMLLIVYYIVIAGFEIRVFAEITMFFLLEGTP 121 KLS +FPGKTVYQY++ + G PG +LL++YYI IAGFEIR +E+TMF+LLEGTP Sbjct: 67 VKLSLRFPGKTVYQYTQIVIGRYPGILWCLLLVLYYIAIAGFEIRAMSEVTMFYLLEGTP 126 Query: 122 IWAIVIPFIWVGAYLVFGGINSIARVYQIIFPVSIFVFLLCYFFSGRLFDINHLRPFLGD 181 IWAI IPFIWVGAYLV GGIN+IARV QI+FP+S+F+ ++ Y S RLF +++LRP LG+ Sbjct: 127 IWAITIPFIWVGAYLVRGGINAIARVGQIVFPISLFILIVSYLLSLRLFHLDNLRPVLGN 186 Query: 182 GIMPVIRGLKSTILVYTGCEVVMTLTAFMQHPQQAVKAMLAGIGIPMALYLITVVMSIGG 241 GI PVI+GLKST+LVY+GCEVVMTL A + +P+QA+KAML GI IP LY IT++M IGG Sbjct: 187 GIFPVIQGLKSTVLVYSGCEVVMTLVAKLDNPKQALKAMLIGISIPAGLYFITIIMVIGG 246 Query: 242 LSIDSVITSTWPTIDLIRSFEVSGFFFERLEFPLLVIWMMQMXXXXXXXXXXXXLGISQL 301 LSIDS +TSTWPTIDLIRSFE++GF FER EFPL+VIW+MQM LG+SQL Sbjct: 247 LSIDSAVTSTWPTIDLIRSFEITGFLFERFEFPLMVIWLMQMFCNYSSFFYNSSLGVSQL 306 Query: 302 YKLKMAPIIFGLMPLIFLATMVPVRINDVFALGDAIGFTGXXXXXXXXXXXXXXXXVRRK 361 + L +IF L+P IFLA MVP IN+VF LGD IG+ +R K Sbjct: 307 FNLPYKSVIFALIPFIFLAAMVPKNINEVFMLGDDIGYFSILLFILFPVSLTLIALIRSK 366 Query: 362 GLKQN 366 G Q+ Sbjct: 367 GGPQH 371 >B7HWN7.1 B7HWN7_BACC7 Spore germination protein A2 Length = 363 Score = 349 bits (896), Expect = 2e-94, Method: Compositional matrix adjust. Identities = 171/333 (51%), Positives = 228/333 (68%) Query: 6 DQKITSTQAAIFLTNSVLGAGILTLPRSVTESAKTPDAWLSXXXXXXXXXXXXXXXXKLS 65 +IT++QA + + N +LG GILTLPR+ E KTPD WLS KLS Sbjct: 6 KDRITTSQAVVIIVNYILGTGILTLPRASVEKVKTPDVWLSVILGGILAMISGVIMVKLS 65 Query: 66 QQFPGKTVYQYSRQIAGSIPGSFLSMLLIVYYIVIAGFEIRVFAEITMFFLLEGTPIWAI 125 QQFP KT YQYS+ I G G LS L+I Y++ + F+IR AE+ FFLLEGTP WAI Sbjct: 66 QQFPEKTFYQYSQDIVGKWIGRLLSFLIIGYFLTTSAFQIRSMAEVISFFLLEGTPTWAI 125 Query: 126 VIPFIWVGAYLVFGGINSIARVYQIIFPVSIFVFLLCYFFSGRLFDINHLRPFLGDGIMP 185 V+PF+W+G YL+ GGINSIAR+++IIFP+++F+FL+ F S +F+I++LRP LG GI P Sbjct: 126 VMPFMWIGLYLIMGGINSIARMFEIIFPITVFIFLIISFMSIGIFEIDNLRPVLGLGIKP 185 Query: 186 VIRGLKSTILVYTGCEVVMTLTAFMQHPQQAVKAMLAGIGIPMALYLITVVMSIGGLSID 245 V+ G+K+T L YTG E+++ L FM+ ++AVKA+L GI IP+ Y+ITVVM IG LSID Sbjct: 186 VLDGIKTTSLAYTGPEIMLILLVFMEQRKKAVKAILVGISIPLIFYVITVVMVIGALSID 245 Query: 246 SVITSTWPTIDLIRSFEVSGFFFERLEFPLLVIWMMQMXXXXXXXXXXXXLGISQLYKLK 305 V+T TWPTIDL+RSFE+SG FER E LLVIW+MQ+ LG++QL+K Sbjct: 246 GVVTRTWPTIDLMRSFEISGLIFERFESLLLVIWIMQIFATYTISYYAAALGLAQLFKKS 305 Query: 306 MAPIIFGLMPLIFLATMVPVRINDVFALGDAIG 338 + P IF L+P+I++ M IND+ LGD +G Sbjct: 306 IHPFIFALIPIIYIIAMTSKNINDLLKLGDMLG 338 >C3C492.1 C3C492_BACTU Spore germination protein Length = 363 Score = 349 bits (896), Expect = 2e-94, Method: Compositional matrix adjust. Identities = 171/333 (51%), Positives = 228/333 (68%) Query: 6 DQKITSTQAAIFLTNSVLGAGILTLPRSVTESAKTPDAWLSXXXXXXXXXXXXXXXXKLS 65 +IT++QA + + N +LG GILTLPR+ E KTPD WLS KLS Sbjct: 6 KDRITTSQAVVIIVNYILGTGILTLPRASVEKVKTPDVWLSVILGGILAMISGVIMVKLS 65 Query: 66 QQFPGKTVYQYSRQIAGSIPGSFLSMLLIVYYIVIAGFEIRVFAEITMFFLLEGTPIWAI 125 QQFP KT YQYS+ I G G LS L+I Y++ + F+IR AE+ FFLLEGTP WAI Sbjct: 66 QQFPEKTFYQYSQDIVGKWIGRLLSFLIIGYFLTTSAFQIRSMAEVISFFLLEGTPTWAI 125 Query: 126 VIPFIWVGAYLVFGGINSIARVYQIIFPVSIFVFLLCYFFSGRLFDINHLRPFLGDGIMP 185 V+PF+W+G YL+ GGINSIAR+++IIFP+++F+FL+ F S +F+I++LRP LG GI P Sbjct: 126 VMPFMWIGLYLIMGGINSIARMFEIIFPITVFIFLIISFMSIGIFEIDNLRPVLGLGIKP 185 Query: 186 VIRGLKSTILVYTGCEVVMTLTAFMQHPQQAVKAMLAGIGIPMALYLITVVMSIGGLSID 245 V+ G+K+T L YTG E+++ L FM+ ++AVKA+L GI IP+ Y+ITVVM IG LSID Sbjct: 186 VLDGIKTTSLAYTGPEIMLILLVFMEQRKKAVKAILVGISIPLIFYVITVVMVIGALSID 245 Query: 246 SVITSTWPTIDLIRSFEVSGFFFERLEFPLLVIWMMQMXXXXXXXXXXXXLGISQLYKLK 305 V+T TWPTIDL+RSFE+SG FER E LLVIW+MQ+ LG++QL+K Sbjct: 246 GVVTRTWPTIDLMRSFEISGLIFERFESLLLVIWIMQIFATYTISYYAAALGLAQLFKKS 305 Query: 306 MAPIIFGLMPLIFLATMVPVRINDVFALGDAIG 338 + P IF L+P+I++ M IND+ LGD +G Sbjct: 306 IHPFIFALIPIIYIIAMTSKNINDLLKLGDMLG 338 >Q7BYC0.1 Q7BYC0_BACCR Spore germination protein BB Length = 363 Score = 349 bits (896), Expect = 2e-94, Method: Compositional matrix adjust. Identities = 171/333 (51%), Positives = 228/333 (68%) Query: 6 DQKITSTQAAIFLTNSVLGAGILTLPRSVTESAKTPDAWLSXXXXXXXXXXXXXXXXKLS 65 +IT++QA + + N +LG GILTLPR+ E KTPD WLS KLS Sbjct: 6 KDRITTSQAVVIIVNYILGTGILTLPRASVEKVKTPDVWLSVILGGILAMVSGVIMVKLS 65 Query: 66 QQFPGKTVYQYSRQIAGSIPGSFLSMLLIVYYIVIAGFEIRVFAEITMFFLLEGTPIWAI 125 QQFP KT YQYS+ I G G LS L+I Y++ + F+IR AE+ FFLLEGTP WAI Sbjct: 66 QQFPEKTFYQYSQDIVGKWIGRLLSFLIIGYFLTTSAFQIRSMAEVISFFLLEGTPTWAI 125 Query: 126 VIPFIWVGAYLVFGGINSIARVYQIIFPVSIFVFLLCYFFSGRLFDINHLRPFLGDGIMP 185 V+PF+W+G YL+ GGINSIAR+++IIFP+++F+FL+ F S +F+I++LRP LG GI P Sbjct: 126 VMPFMWIGLYLIMGGINSIARMFEIIFPITVFIFLIISFMSIGIFEIDNLRPVLGLGIKP 185 Query: 186 VIRGLKSTILVYTGCEVVMTLTAFMQHPQQAVKAMLAGIGIPMALYLITVVMSIGGLSID 245 V+ G+K+T L YTG E+++ L FM+ ++AVKA+L GI IP+ Y+ITVVM IG LSID Sbjct: 186 VLDGIKTTSLAYTGPEIMLILLVFMEQRKKAVKAILVGISIPLIFYVITVVMVIGALSID 245 Query: 246 SVITSTWPTIDLIRSFEVSGFFFERLEFPLLVIWMMQMXXXXXXXXXXXXLGISQLYKLK 305 V+T TWPTIDL+RSFE+SG FER E LLVIW+MQ+ LG++QL+K Sbjct: 246 GVVTRTWPTIDLMRSFEISGLIFERFESLLLVIWIMQIFATYTISYYAAALGLAQLFKKS 305 Query: 306 MAPIIFGLMPLIFLATMVPVRINDVFALGDAIG 338 + P IF L+P+I++ M IND+ LGD +G Sbjct: 306 IHPFIFALIPIIYIIAMTSKNINDLLKLGDMLG 338 >Q9K337.1 Q9K337_BACCE YndE protein Length = 363 Score = 349 bits (896), Expect = 2e-94, Method: Compositional matrix adjust. Identities = 171/333 (51%), Positives = 228/333 (68%) Query: 6 DQKITSTQAAIFLTNSVLGAGILTLPRSVTESAKTPDAWLSXXXXXXXXXXXXXXXXKLS 65 +IT++QA + + N +LG GILTLPR+ E KTPD WLS KLS Sbjct: 6 KDRITTSQAVVIIVNYILGTGILTLPRASVEKVKTPDVWLSVILGGILAMVSGVIMVKLS 65 Query: 66 QQFPGKTVYQYSRQIAGSIPGSFLSMLLIVYYIVIAGFEIRVFAEITMFFLLEGTPIWAI 125 QQFP KT YQYS+ I G G LS L+I Y++ + F+IR AE+ FFLLEGTP WAI Sbjct: 66 QQFPEKTFYQYSQDIVGKWIGRLLSFLIIGYFLTTSAFQIRSMAEVISFFLLEGTPTWAI 125 Query: 126 VIPFIWVGAYLVFGGINSIARVYQIIFPVSIFVFLLCYFFSGRLFDINHLRPFLGDGIMP 185 V+PF+W+G YL+ GGINSIAR+++IIFP+++F+FL+ F S +F+I++LRP LG GI P Sbjct: 126 VMPFMWIGLYLIMGGINSIARMFEIIFPITVFIFLIISFMSIGIFEIDNLRPVLGLGIKP 185 Query: 186 VIRGLKSTILVYTGCEVVMTLTAFMQHPQQAVKAMLAGIGIPMALYLITVVMSIGGLSID 245 V+ G+K+T L YTG E+++ L FM+ ++AVKA+L GI IP+ Y+ITVVM IG LSID Sbjct: 186 VLDGIKTTSLAYTGPEIMLILLVFMEQRKKAVKAILVGISIPLIFYVITVVMVIGALSID 245 Query: 246 SVITSTWPTIDLIRSFEVSGFFFERLEFPLLVIWMMQMXXXXXXXXXXXXLGISQLYKLK 305 V+T TWPTIDL+RSFE+SG FER E LLVIW+MQ+ LG++QL+K Sbjct: 246 GVVTRTWPTIDLMRSFEISGLIFERFESLLLVIWIMQIFATYTISYYAAALGLAQLFKKS 305 Query: 306 MAPIIFGLMPLIFLATMVPVRINDVFALGDAIG 338 + P IF L+P+I++ M IND+ LGD +G Sbjct: 306 IHPFIFALIPIIYIIAMTSKNINDLLKLGDMLG 338 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.331 0.145 0.439 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,741,585,368 Number of extensions: 62660364 Number of successful extensions: 206362 Number of sequences better than 1.0e-05: 800 Number of HSP's gapped: 205395 Number of HSP's successfully gapped: 809 Length of query: 367 Length of database: 3,060,696,203 Length adjustment: 137 Effective length of query: 230 Effective length of database: 1,770,017,148 Effective search space: 407103944040 Effective search space used: 407103944040 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.9 bits) S2: 132 (55.5 bits)