BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0340.0041.p (536 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1DB11.1 B1DB11_9BACL GerA spore germination protein 585 e-165 B3K870.1 B3K870_9BACI GerA spore germination protein 561 e-158 B7JE63.1 B7JE63_BACC0 Spore germination protein GerAA 548 e-154 Q4MX50.1 Q4MX50_BACCE YndD 548 e-154 B3YN24.1 B3YN24_BACCE Spore germination protein GerAA 548 e-154 >B1DB11.1 B1DB11_9BACL GerA spore germination protein Length = 537 Score = 585 bits (1508), Expect = e-165, Method: Compositional matrix adjust. Identities = 300/540 (55%), Positives = 380/540 (70%), Gaps = 25/540 (4%) Query: 2 QAAITLLCPLGIYFIYKKLYAF-----VSSAGPKMKAIAQPNAESTADSLVSGQYEADLS 56 QA ITL+ PL IY IYK L + VSS G + +++ ++ Y+A+L Sbjct: 17 QALITLVVPLLIYRIYKALASLANDKLVSSGG---ASSVDASSDEPIIHPITADYDANLD 73 Query: 57 YVQQAIGGNSDVNFREFVVKGYELRAVLVFVNGMQDEELINRHVMQQLMSGNGGMKEEKQ 116 ++ IG NSDV RE + ++RA L+FV GMQ+++++ +MQ L Sbjct: 74 KIRNTIGVNSDVVIRELTMGDAKVRAALIFVKGMQEDQVLKMQIMQML------------ 121 Query: 117 DNRET-LTLSALKSNLLPLTELEEVKELDKLQERILFGYTALLIEGIPAGLLVGQPNGSI 175 R+T L+L ++ LP + +++L + ILFGY AL+I+G GLL+ P+G Sbjct: 122 --RDTELSLPIIER--LPTNAAHRISSMEELTKAILFGYLALMIDGRDEGLLINPPSGPA 177 Query: 176 RAITEPTSEALLRGPRIAFSEVLSENTSMLRRQGLNKDLEMKLFEVGSLVKRKLVIAYMK 235 R I+EP SE+LLRG RI F+E ++ NT+MLR+QG+ ++L+++ F VG ++++L I Y+ Sbjct: 178 RDISEPVSESLLRGSRIGFTEDINVNTAMLRKQGVTENLQVRSFMVGHTIQKELAILYLD 237 Query: 236 DIVNPELLEEVKTRVSRIDMDFIAESGYVEQLIEDNILSPFQQIQNTERPDRVINALLEG 295 IVNP+LL EV R+ +I +D+ AESGY+EQLIEDN LSPFQQ QNTERPDRVI ALLEG Sbjct: 238 QIVNPDLLREVIGRIEKIKLDYAAESGYIEQLIEDNYLSPFQQTQNTERPDRVIAALLEG 297 Query: 296 RVALLLDGTPFALIVPVTFSMLLQSPEDYFERWLPGTXXXXXXXXXXXXXXXXXXXYISF 355 R+A+LLDGTPFALIVPVTFSMLLQSPEDYFERWLP + YISF Sbjct: 298 RIAILLDGTPFALIVPVTFSMLLQSPEDYFERWLPSSLLRMLRFLAAFIALMAPSLYISF 357 Query: 356 ISFHPGLIPTELVISIIETRQGVPFPXXXXXXXXXXXXXXXREAGIRLPKPIGPAMGIVG 415 ISFHPGLIPTEL ++IIETRQGVPFP REAGIRLPKPIGPAMGIVG Sbjct: 358 ISFHPGLIPTELALTIIETRQGVPFPALIEVMILEIAIEILREAGIRLPKPIGPAMGIVG 417 Query: 416 GLIIGDAAVNAGIVSPFLVIVVAVTAISSFSIPMYSAGITLRILRFVGMMFAAVLGMLGT 475 GL+IG+AAV AGIVSPFLVIVVAVTAISSFSIPMYSAGITLR+LRF M+FA++LGM GT Sbjct: 418 GLVIGEAAVQAGIVSPFLVIVVAVTAISSFSIPMYSAGITLRLLRFAAMLFASILGMFGT 477 Query: 476 ILFMLLIFIHLTKLKSFGVPYVTPFSPLRLSDWKDLYIRAPLPLMKRRPEMMKTQKKKRR 535 ILF LLI HL+KLKSFGVPY+TP +P R SDWKD ++R P+ +MKRRP++MKT + KRR Sbjct: 478 ILFFLLICCHLSKLKSFGVPYLTPLTPFRWSDWKDFFVRVPIFMMKRRPKLMKTMQNKRR 537 >B3K870.1 B3K870_9BACI GerA spore germination protein Length = 555 Score = 561 bits (1447), Expect = e-158, Method: Compositional matrix adjust. Identities = 286/541 (52%), Positives = 376/541 (69%), Gaps = 8/541 (1%) Query: 2 QAAITLLCPLGIYFIYKKLYAFVSSA---GPKMKAIAQPNAESTADSLVSGQYEADLSYV 58 QA + P+ + K + ++S+ P + + + D+ ++G Y+ D++ V Sbjct: 17 QAFLLFFVPVVMLMFSKWFHKSINSSFSLKPNIPSSPVREDSVSPDASLTGLYDTDITSV 76 Query: 59 QQAIGGNSDVNFREFVVKGYELRAVLVFVNGMQDEELINRHVMQQLMSGNGGMKEEKQDN 118 +QAIG N+DV REF ++G RAV+V+V+G+ D +LI+ H+++ LM G+ E + DN Sbjct: 77 RQAIGDNADVEIREFTIRGTRTRAVVVYVDGLLDSDLIDTHLIRPLMLE--GVPETEHDN 134 Query: 119 ---RETLTLSALKSNLLPLTELEEVKELDKLQERILFGYTALLIEGIPAGLLVGQPNGSI 175 + + S +LP+ E++ V++L L + +L G LL++G+ LVG G Sbjct: 135 FNDSSDAIKAYISSQMLPVCEIKHVRQLLDLVQAVLAGTPCLLVDGMQGAFLVGSAKGKS 194 Query: 176 RAITEPTSEALLRGPRIAFSEVLSENTSMLRRQGLNKDLEMKLFEVGSLVKRKLVIAYMK 235 R + EP SEALLRGPRI F+E LS+NT +LR G N+ L++ FEVG+ VK+ LV+AYMK Sbjct: 195 RNVEEPLSEALLRGPRIGFTERLSDNTGLLRLHGKNESLQLTRFEVGNRVKKDLVVAYMK 254 Query: 236 DIVNPELLEEVKTRVSRIDMDFIAESGYVEQLIEDNILSPFQQIQNTERPDRVINALLEG 295 DI +P L++EV TR+ +I++D ESGY+EQLIE+N++SPFQQIQNTERPDRVI ALLEG Sbjct: 255 DIADPNLVDEVVTRIRKIEIDDPLESGYIEQLIEENVMSPFQQIQNTERPDRVIGALLEG 314 Query: 296 RVALLLDGTPFALIVPVTFSMLLQSPEDYFERWLPGTXXXXXXXXXXXXXXXXXXXYISF 355 RVA+LLDGTP+ALIVPVTFSMLLQSPEDY+ERW+ G+ YISF Sbjct: 315 RVAILLDGTPYALIVPVTFSMLLQSPEDYYERWISGSFVRLLRFLSALLALLAPALYISF 374 Query: 356 ISFHPGLIPTELVISIIETRQGVPFPXXXXXXXXXXXXXXXREAGIRLPKPIGPAMGIVG 415 ISF+PGLIPT+L ++II TRQGVPFP REAGIRLPKPIGPAMGIVG Sbjct: 375 ISFNPGLIPTKLALTIIGTRQGVPFPSIIEALIMEIAIEILREAGIRLPKPIGPAMGIVG 434 Query: 416 GLIIGDAAVNAGIVSPFLVIVVAVTAISSFSIPMYSAGITLRILRFVGMMFAAVLGMLGT 475 GLIIGDAAVNAGIVSPFLVIVVAVTAISSF+IP+YSAGITLR+LRF M FAAVLG+ G Sbjct: 435 GLIIGDAAVNAGIVSPFLVIVVAVTAISSFTIPVYSAGITLRVLRFTAMFFAAVLGLFGV 494 Query: 476 ILFMLLIFIHLTKLKSFGVPYVTPFSPLRLSDWKDLYIRAPLPLMKRRPEMMKTQKKKRR 535 ++F LL+ HL +LKSFGVPY +P P ++D KD IR PL +MK+RP+++K Q RR Sbjct: 495 VMFFLLLCSHLVRLKSFGVPYASPMVPYGINDLKDFLIRFPLKVMKKRPQLLKPQDPDRR 554 Query: 536 T 536 Sbjct: 555 N 555 >B7JE63.1 B7JE63_BACC0 Spore germination protein GerAA Length = 524 Score = 548 bits (1413), Expect = e-154, Method: Compositional matrix adjust. Identities = 271/507 (53%), Positives = 358/507 (70%), Gaps = 3/507 (0%) Query: 30 KMKAIAQPNAESTADSLVSGQYEADLSYVQQAIGGNSDVNFREFVVKGYELRAVLVFVNG 89 K K +P+ +T + + ++L ++Q IG NSDV FREF + +++A ++FV+G Sbjct: 19 KTKQEHKPSQNTTVH--FTNDFASNLELIKQQIGHNSDVRFREFNIGRTDIQAGIIFVDG 76 Query: 90 MQDEELINRHVMQQLMSG-NGGMKEEKQDNRETLTLSALKSNLLPLTELEEVKELDKLQE 148 + D+E+I++H+M+ LM + K E T++ +K+ +L ++E+ EV+ + L Sbjct: 77 LSDKEIIDKHIMKLLMDDFSDEYKNESYYVEGTVSKEYIKNKVLTISEVAEVQYIKDLIP 136 Query: 149 RILFGYTALLIEGIPAGLLVGQPNGSIRAITEPTSEALLRGPRIAFSEVLSENTSMLRRQ 208 ++L G TALLI+G+ ++G + R + EP SEAL+RGPR+ F+EVLS+NTS+LRR Sbjct: 137 KVLIGSTALLIDGLADVFILGTTKANKRNMEEPVSEALVRGPRVGFTEVLSDNTSLLRRH 196 Query: 209 GLNKDLEMKLFEVGSLVKRKLVIAYMKDIVNPELLEEVKTRVSRIDMDFIAESGYVEQLI 268 +++L + VG K++LV+A++KD+ + EL+EE+K R+ +ID+D + ESGY+EQLI Sbjct: 197 CADENLSLVKLPVGERAKKELVVAFIKDLASTELVEEIKNRIQKIDIDSVLESGYIEQLI 256 Query: 269 EDNILSPFQQIQNTERPDRVINALLEGRVALLLDGTPFALIVPVTFSMLLQSPEDYFERW 328 EDN LSPF QIQNTERPDRVI+AL+EGRVA+LLDGTPF LI PVTFSMLLQSPEDY+ERW Sbjct: 257 EDNYLSPFPQIQNTERPDRVISALMEGRVAILLDGTPFVLIAPVTFSMLLQSPEDYYERW 316 Query: 329 LPGTXXXXXXXXXXXXXXXXXXXYISFISFHPGLIPTELVISIIETRQGVPFPXXXXXXX 388 LPGT YISFISFHPGLIPT+L ISI+ +R+GVPFP Sbjct: 317 LPGTLLRLLRFMTAFVSLFAPALYISFISFHPGLIPTKLAISILGSREGVPFPALIEALI 376 Query: 389 XXXXXXXXREAGIRLPKPIGPAMGIVGGLIIGDAAVNAGIVSPFLVIVVAVTAISSFSIP 448 REAG+RLPKPIGPAMGIVGGLIIG AAV AGIVSP +VIVVAVTAISSF+IP Sbjct: 377 MEVAIEVLREAGLRLPKPIGPAMGIVGGLIIGQAAVEAGIVSPIMVIVVAVTAISSFTIP 436 Query: 449 MYSAGITLRILRFVGMMFAAVLGMLGTILFMLLIFIHLTKLKSFGVPYVTPFSPLRLSDW 508 YS GITLRILRFV M AA+ G+ G ILF LL+ HL KLKSFGVPY +P P R SDW Sbjct: 437 QYSVGITLRILRFVAMFCAAIFGLYGVILFFLLLCSHLVKLKSFGVPYTSPAVPYRFSDW 496 Query: 509 KDLYIRAPLPLMKRRPEMMKTQKKKRR 535 KD +R PL +M+RRP++M T+K R+ Sbjct: 497 KDFMVRMPLKIMRRRPKIMHTKKTNRK 523 >Q4MX50.1 Q4MX50_BACCE YndD Length = 524 Score = 548 bits (1412), Expect = e-154, Method: Compositional matrix adjust. Identities = 273/507 (53%), Positives = 358/507 (70%), Gaps = 3/507 (0%) Query: 30 KMKAIAQPNAESTADSLVSGQYEADLSYVQQAIGGNSDVNFREFVVKGYELRAVLVFVNG 89 K K +P+ +T + + ++L ++Q IG NSDV FR+F + +++A ++FV+G Sbjct: 19 KTKQEYKPSQNTTVH--FTNDFASNLEIIKQQIGHNSDVRFRKFNIGRTDIQAGIIFVDG 76 Query: 90 MQDEELINRHVMQQLMSG-NGGMKEEKQDNRETLTLSALKSNLLPLTELEEVKELDKLQE 148 + D+E+I++H+M+ LM + K E T++ +K+ +L ++E+ EV + L Sbjct: 77 LSDKEIIDKHIMKLLMDDFSDEYKNESSYVEGTVSKEYIKNKVLTISEVAEVHYIKDLIS 136 Query: 149 RILFGYTALLIEGIPAGLLVGQPNGSIRAITEPTSEALLRGPRIAFSEVLSENTSMLRRQ 208 ++L G TALLI+G+ ++G + R I EP SEAL+RGPR+ F+EVLS+NTS+LRR Sbjct: 137 KVLIGSTALLIDGLADVFILGTTKVNKRNIEEPVSEALVRGPRVGFTEVLSDNTSLLRRH 196 Query: 209 GLNKDLEMKLFEVGSLVKRKLVIAYMKDIVNPELLEEVKTRVSRIDMDFIAESGYVEQLI 268 +++L + VG K++LVIA++KD+ + EL+EE+K+R+ +ID+D + ESGY+EQLI Sbjct: 197 CADENLSLVKLPVGERAKKELVIAFIKDLASTELVEEIKSRIQKIDIDSVLESGYIEQLI 256 Query: 269 EDNILSPFQQIQNTERPDRVINALLEGRVALLLDGTPFALIVPVTFSMLLQSPEDYFERW 328 EDN LSPF QIQNTERPDRVI+AL+EGRVA+LLDGTPF LI PVTFSMLLQSPEDY+ERW Sbjct: 257 EDNYLSPFPQIQNTERPDRVISALMEGRVAILLDGTPFVLIAPVTFSMLLQSPEDYYERW 316 Query: 329 LPGTXXXXXXXXXXXXXXXXXXXYISFISFHPGLIPTELVISIIETRQGVPFPXXXXXXX 388 LPGT YISFISFHPGLIPT+L ISII +R+GVPFP Sbjct: 317 LPGTLLRLLRFMTAFVSLFAPALYISFISFHPGLIPTKLAISIIGSREGVPFPALIEALI 376 Query: 389 XXXXXXXXREAGIRLPKPIGPAMGIVGGLIIGDAAVNAGIVSPFLVIVVAVTAISSFSIP 448 REAG+RLPKPIGPAMGIVGGLIIG AAV AGIVSP +VIVVAVTAISSF+IP Sbjct: 377 MEIAIEVLREAGLRLPKPIGPAMGIVGGLIIGQAAVEAGIVSPIMVIVVAVTAISSFTIP 436 Query: 449 MYSAGITLRILRFVGMMFAAVLGMLGTILFMLLIFIHLTKLKSFGVPYVTPFSPLRLSDW 508 YS GITLRILRFV M AA+ G+ G ILF LL+ HL KLKSFGVPY +P P R SDW Sbjct: 437 QYSVGITLRILRFVAMFCAAIFGLYGVILFFLLLCSHLVKLKSFGVPYTSPAVPYRFSDW 496 Query: 509 KDLYIRAPLPLMKRRPEMMKTQKKKRR 535 KD +R PL +M+RRP++M T+K R+ Sbjct: 497 KDFMVRMPLKIMRRRPKIMHTKKTIRK 523 >B3YN24.1 B3YN24_BACCE Spore germination protein GerAA Length = 524 Score = 548 bits (1412), Expect = e-154, Method: Compositional matrix adjust. Identities = 271/507 (53%), Positives = 357/507 (70%), Gaps = 3/507 (0%) Query: 30 KMKAIAQPNAESTADSLVSGQYEADLSYVQQAIGGNSDVNFREFVVKGYELRAVLVFVNG 89 K K +P+ +T + + ++L ++Q IG NSDV FREF + +++A ++FV+G Sbjct: 19 KTKQEHKPSQNTTVH--FTNDFASNLELIKQQIGHNSDVRFREFNIGRTDIQAGIIFVDG 76 Query: 90 MQDEELINRHVMQQLMSG-NGGMKEEKQDNRETLTLSALKSNLLPLTELEEVKELDKLQE 148 + D+E+I++H+M+ LM + K E T++ +K+ +L ++E+ EV + L Sbjct: 77 LSDKEIIDKHIMKLLMDDFSDEYKNESYYVEGTVSKEYIKNKVLTISEVAEVHYIKDLIP 136 Query: 149 RILFGYTALLIEGIPAGLLVGQPNGSIRAITEPTSEALLRGPRIAFSEVLSENTSMLRRQ 208 ++L G TALLI+G+ ++G + R + EP SEAL+RGPR+ F+EVLS+NTS+LRR Sbjct: 137 KVLIGSTALLIDGLADVFILGTTKANKRNMEEPVSEALVRGPRVGFTEVLSDNTSLLRRH 196 Query: 209 GLNKDLEMKLFEVGSLVKRKLVIAYMKDIVNPELLEEVKTRVSRIDMDFIAESGYVEQLI 268 +++L + VG K++LV+A++KD+ + EL+EE+K R+ +ID+D + ESGY+EQLI Sbjct: 197 CADENLSLVKLPVGERAKKELVVAFIKDLASTELVEEIKNRIQKIDIDSVLESGYIEQLI 256 Query: 269 EDNILSPFQQIQNTERPDRVINALLEGRVALLLDGTPFALIVPVTFSMLLQSPEDYFERW 328 EDN LSPF QIQNTERPDRVI+AL+EGRVA+LLDGTPF LI PVTFSMLLQSPEDY+ERW Sbjct: 257 EDNYLSPFPQIQNTERPDRVISALMEGRVAILLDGTPFVLIAPVTFSMLLQSPEDYYERW 316 Query: 329 LPGTXXXXXXXXXXXXXXXXXXXYISFISFHPGLIPTELVISIIETRQGVPFPXXXXXXX 388 LPGT YISFISFHPGLIPT+L ISI+ +R+GVPFP Sbjct: 317 LPGTLLRLLRFMTAFVSLFAPALYISFISFHPGLIPTKLAISILGSREGVPFPALIEALI 376 Query: 389 XXXXXXXXREAGIRLPKPIGPAMGIVGGLIIGDAAVNAGIVSPFLVIVVAVTAISSFSIP 448 REAG+RLPKPIGPAMGIVGGLIIG AAV AGIVSP +VIVVAVTAISSF+IP Sbjct: 377 MEVAIEVLREAGLRLPKPIGPAMGIVGGLIIGQAAVEAGIVSPIMVIVVAVTAISSFTIP 436 Query: 449 MYSAGITLRILRFVGMMFAAVLGMLGTILFMLLIFIHLTKLKSFGVPYVTPFSPLRLSDW 508 YS GITLRILRFV M AA+ G+ G ILF LL+ HL KLKSFGVPY +P P R SDW Sbjct: 437 QYSVGITLRILRFVAMFCAAIFGLYGVILFFLLLCSHLVKLKSFGVPYTSPAVPYRFSDW 496 Query: 509 KDLYIRAPLPLMKRRPEMMKTQKKKRR 535 KD +R PL +M+RRP++M T+K R+ Sbjct: 497 KDFMVRMPLKIMRRRPKIMHTKKTNRK 523 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.322 0.140 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,836,620,780 Number of extensions: 112087941 Number of successful extensions: 330940 Number of sequences better than 1.0e-05: 1090 Number of HSP's gapped: 328677 Number of HSP's successfully gapped: 1102 Length of query: 536 Length of database: 3,060,696,203 Length adjustment: 141 Effective length of query: 395 Effective length of database: 1,732,333,088 Effective search space: 684271569760 Effective search space used: 684271569760 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 134 (56.2 bits)