BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0340.0024.p (243 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1DGH1.1 B1DGH1_9BACL Beta-galactosidase EC=3.2.1.23 298 3e-79 B3K4X6.1 B3K4X6_9BACI Glycoside hydrolase family 2 TIM barrel 293 1e-77 C1KC07.1 C1KC07_9BACL Beta-galactosidase (Fragment) 230 1e-58 A3FFK4.1 A3FFK4_BACME Beta-galactosidase 228 3e-58 O85167.1 O85167_BACME Beta-galactosidase 224 5e-57 >B1DGH1.1 B1DGH1_9BACL Beta-galactosidase EC=3.2.1.23 Length = 1037 Score = 298 bits (763), Expect = 3e-79, Method: Compositional matrix adjust. Identities = 138/243 (56%), Positives = 168/243 (69%) Query: 1 VRPNFWRAVTDNDLGNGLDKRCAVWKDASYSRTLTGFSYTAGDHLCTVAVSYQLETQPAS 60 +RPNFWRA TDND GN + +RC++WKDA ++ L F++TA C V +QL T P S Sbjct: 795 IRPNFWRAATDNDFGNKMPERCSIWKDAGSTKRLIQFTWTAAQDRCHVTTVHQLATNPVS 854 Query: 61 TLLLQYKITPDGKVDITQELNPGSDGLPEIPXXXXXXXXXXXXXTVSWYGKGPHENYWDR 120 L + Y + +G +DI ++L PG D LPEIP T+SWYG GP +NYWDR Sbjct: 855 LLTIGYTVHSNGSIDIHEQLLPGGDKLPEIPEFGMLLVLDGNLDTISWYGLGPQDNYWDR 914 Query: 121 QTSAKLGHYTGKVSEQFVHYLRPQECGNKTGVRYASLTESVSGNGIHLESTLPFEVNVLP 180 +T AKLG Y+G V +QFV YLRPQECGNKT VRYA LT GNGI +S E+N LP Sbjct: 915 KTGAKLGQYSGTVKDQFVPYLRPQECGNKTDVRYAELTNGADGNGIRFDSAAVMEINALP 974 Query: 181 WTPEELEASDHPYKLAPSTKSVLRVNYKQMGVGGDDSWGAPTHAEFTLPSNRPYSFRFTL 240 WTP+ELEASDHPYKL S K+V+RVN KQMGVGGDDSWGAP H E+ L +NR YS FTL Sbjct: 975 WTPDELEASDHPYKLPVSFKTVVRVNDKQMGVGGDDSWGAPIHPEYKLFANRTYSLSFTL 1034 Query: 241 SVV 243 S++ Sbjct: 1035 SLI 1037 >B3K4X6.1 B3K4X6_9BACI Glycoside hydrolase family 2 TIM barrel Length = 1042 Score = 293 bits (749), Expect = 1e-77, Method: Compositional matrix adjust. Identities = 142/240 (59%), Positives = 169/240 (70%), Gaps = 1/240 (0%) Query: 1 VRPNFWRAVTDNDLGNGLDKRCAVWKDASYSRTLTGFSYTAGDHLCTVAVSYQLETQPAS 60 RPNFWRAVTDNDLGN L +R VW+ A R L + A V+ Y++ET P S Sbjct: 796 ARPNFWRAVTDNDLGNRLPERSGVWRTAHDRRKLLSLRWHAEGPAVVVSSHYRIETNPVS 855 Query: 61 TLLLQYKITPDGKVDITQELNPGSDGLPEIPXXXXXXXXXXXXXTVSWYGKGPHENYWDR 120 +L L+Y+I PDG V + + L PG+ GLP+IP T+SWYG+GPH+NYWDR Sbjct: 856 SLTLEYRIYPDGTVRVHETLIPGA-GLPDIPEVGMLFLLDEGLDTLSWYGRGPHDNYWDR 914 Query: 121 QTSAKLGHYTGKVSEQFVHYLRPQECGNKTGVRYASLTESVSGNGIHLESTLPFEVNVLP 180 +T AK+G Y GKV++QFV YLRPQECGNKT VR+A LT G+G+ ES+ EV LP Sbjct: 915 KTGAKIGRYEGKVADQFVPYLRPQECGNKTDVRFALLTGESGGDGLEFESSSLMEVCALP 974 Query: 181 WTPEELEASDHPYKLAPSTKSVLRVNYKQMGVGGDDSWGAPTHAEFTLPSNRPYSFRFTL 240 WTPEELE SDH YKL PS K+VLR+NYKQMGVGGDDSWGAP H EFTLPSNR YSF FT+ Sbjct: 975 WTPEELEGSDHAYKLPPSDKTVLRINYKQMGVGGDDSWGAPIHEEFTLPSNRTYSFGFTM 1034 >C1KC07.1 C1KC07_9BACL Beta-galactosidase (Fragment) Length = 1041 Score = 230 bits (586), Expect = 1e-58, Method: Compositional matrix adjust. Identities = 112/239 (46%), Positives = 152/239 (63%), Gaps = 2/239 (0%) Query: 3 PNFWRAVTDNDLGNGLDKRCAVWKDASYSRTLTGFSYTAGDHLCTVAVSYQLETQPASTL 62 PNFWRA+TDND GNGLD+R +W+DA+ R L F Y + +++ + E S + Sbjct: 801 PNFWRAMTDNDRGNGLDQRSQIWRDANEVRELVSFQYEVLTNRVSISTVFLYEDLNHSRV 860 Query: 63 LLQYKITPDGKVDITQELNPGSDGLPEIPXXXXXXXXXXXXXTVSWYGKGPHENYWDRQT 122 L + IT G++ + L PG D LPEIP +SWYGKGPHE+YWD+Q Sbjct: 861 ELNFLITGTGEIKVDYVLKPGED-LPEIPEIGLMLTMPKSFDQLSWYGKGPHESYWDKQK 919 Query: 123 SAKLGHYTGKVSEQFVHYLRPQECGNKTGVRYASLTESVSGNGIHLESTLPFEVNVLPWT 182 AK+G Y G V +Q+V YL+PQECGNK GVR A L V G G+ + E+NVLP+T Sbjct: 920 GAKIGLYQGFVGDQYVPYLKPQECGNKVGVRSAELVNDV-GVGLIISGLPTLELNVLPYT 978 Query: 183 PEELEASDHPYKLAPSTKSVLRVNYKQMGVGGDDSWGAPTHAEFTLPSNRPYSFRFTLS 241 P +LE++DH Y+L + ++V+R+N QMGVGGDDSWG TH +FTL +N+ Y + F L+ Sbjct: 979 PVQLESADHSYQLPETDQTVVRINLGQMGVGGDDSWGQRTHQDFTLFANKTYHYSFMLN 1037 >A3FFK4.1 A3FFK4_BACME Beta-galactosidase Length = 1034 Score = 228 bits (581), Expect = 3e-58, Method: Compositional matrix adjust. Identities = 117/239 (48%), Positives = 149/239 (62%), Gaps = 3/239 (1%) Query: 2 RPNFWRAVTDNDLGNGLDKRCAVWKDASYSRTLTGFSYTAGDHLCTVAVSYQLETQPAST 61 RPNFWRAVTDNDLGN L +RC W+ AS + + S ++V L+ AS Sbjct: 796 RPNFWRAVTDNDLGNKLHERCQTWRQASLEQHVQNISVQPQIDFVIISVELALDNSLASC 855 Query: 62 LLLQYKITPDGKVDITQELNPGSDGLPEIPXXXXXXXXXXXXXTVSWYGKGPHENYWDRQ 121 + Y + DG++ I Q L P S+ +PEIP +++WYG+GPHENYWDR+ Sbjct: 856 YV-TYTLYNDGEMKIEQSLAP-SETMPEIPEIGMLFMMNAAFDSLTWYGRGPHENYWDRK 913 Query: 122 TSAKLGHYTGKVSEQFVHYLRPQECGNKTGVRYASLTESVSGNGIHLESTLPFEVNVLPW 181 T AKL + G V EQ YLRPQECGNKT VR+A++T G G ++ E+N LP+ Sbjct: 914 TGAKLALHKGSVKEQVTPYLRPQECGNKTDVRWATITND-QGRGFLIKGLPTVELNALPY 972 Query: 182 TPEELEASDHPYKLAPSTKSVLRVNYKQMGVGGDDSWGAPTHAEFTLPSNRPYSFRFTL 240 +P ELEA DH YKL S +RVNYKQMGVGGDDSWGA TH ++TL +NR Y+ FTL Sbjct: 973 SPFELEAYDHFYKLPSSDSVTVRVNYKQMGVGGDDSWGAKTHPDYTLYANRSYTNTFTL 1031 >O85167.1 O85167_BACME Beta-galactosidase Length = 1034 Score = 224 bits (571), Expect = 5e-57, Method: Compositional matrix adjust. Identities = 116/239 (48%), Positives = 147/239 (61%), Gaps = 3/239 (1%) Query: 2 RPNFWRAVTDNDLGNGLDKRCAVWKDASYSRTLTGFSYTAGDHLCTVAVSYQLETQPAST 61 RPNFWRAVTDNDLGN L +RC W+ AS + + ++V L+ AS Sbjct: 796 RPNFWRAVTDNDLGNKLHERCQTWRQASLEQHVKKVIVQPQVDFVIISVELALDNSLASC 855 Query: 62 LLLQYKITPDGKVDITQELNPGSDGLPEIPXXXXXXXXXXXXXTVSWYGKGPHENYWDRQ 121 + Y + DGK+ I Q L P S+ +PEIP +++WYG+GPHENYWDR+ Sbjct: 856 YV-TYTLYNDGKMKIEQSLAP-SETMPEIPEIGMLFTMNAAFDSLTWYGRGPHENYWDRK 913 Query: 122 TSAKLGHYTGKVSEQFVHYLRPQECGNKTGVRYASLTESVSGNGIHLESTLPFEVNVLPW 181 T AKL + G V EQ YLRPQECGNKT VR+A++T G G ++ E+N LP+ Sbjct: 914 TGAKLALHKGSVKEQVTPYLRPQECGNKTDVRWATITND-QGRGFLIKGLPTVELNALPY 972 Query: 182 TPEELEASDHPYKLAPSTKSVLRVNYKQMGVGGDDSWGAPTHAEFTLPSNRPYSFRFTL 240 +P ELEA DH YKL S +RVNYKQMGVGGDDSW A TH ++TL +NR Y+ FTL Sbjct: 973 SPFELEAYDHFYKLPASDSVTVRVNYKQMGVGGDDSWQAKTHPDYTLYANRSYTNTFTL 1031 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.314 0.133 0.416 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,533,796,878 Number of extensions: 61284560 Number of successful extensions: 88290 Number of sequences better than 1.0e-05: 664 Number of HSP's gapped: 87342 Number of HSP's successfully gapped: 664 Length of query: 243 Length of database: 3,060,696,203 Length adjustment: 132 Effective length of query: 111 Effective length of database: 1,817,122,223 Effective search space: 201700566753 Effective search space used: 201700566753 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 129 (54.3 bits)