BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0337.0019.p (327 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KE24.1 B3KE24_9BACI Regulatory protein LacI 372 e-101 C3B7Q7.1 C3B7Q7_BACMY Transcriptional regulator, LacI 317 8e-85 C3AQH5.1 C3AQH5_BACMY Transcriptional regulator, LacI 317 8e-85 C3BPV4.1 C3BPV4_9BACI Transcriptional regulator, LacI 315 4e-84 Q81IW5.1 Q81IW5_BACCR Transcriptional regulator, LacI family 315 5e-84 >B3KE24.1 B3KE24_9BACI Regulatory protein LacI Length = 341 Score = 372 bits (955), Expect = e-101, Method: Compositional matrix adjust. Identities = 176/328 (53%), Positives = 244/328 (74%), Gaps = 4/328 (1%) Query: 1 MANIKEIARIAGVSVTTVSRVLNNHPYVSKDKRTAVLQTIEQLNYTRNMNAVHLITGRTG 60 +ANIKEIA++AGVSV+TVSRVLNNHPYV ++KR VL+T++QL Y RNMNAVHLITG+T Sbjct: 17 VANIKEIAKLAGVSVSTVSRVLNNHPYVREEKRARVLETMKQLKYARNMNAVHLITGKTM 76 Query: 61 AVAVILPYISSFYFSVVMDGXXXXXXXXXYRLILCQTNYVPEEELKVLDMLRNKEIDGVI 120 +A++LP+I+ YFS++M+G YRLILCQT Y P++E++VL+MLRN+EIDG+I Sbjct: 77 NIAIMLPFINHAYFSLLMEGAGQEALAASYRLILCQTGYAPDKEVEVLEMLRNREIDGII 136 Query: 121 IVSTALKPEVIESYTIYGPVVTCQNSGERQFSSVYIEHYAAFRHGLEYLYGKGHRRIGYC 180 I+S+A+ P+ IE+Y YGP+V CQ++GER FSS+YI H AFR + YL +GHRRIG+ Sbjct: 137 ILSSAVTPKQIEAYCAYGPIVCCQDAGERLFSSIYINHAEAFRSAMRYLIRRGHRRIGFT 196 Query: 181 EGRQNGSSASIRQNAFQDFLAEKELLFDPEWMIFDCMREEDGAEVARRLLDNPDRSGRPE 240 GR + S RQ A+++ L E + EW++ C+ EDGA + RL + RP Sbjct: 197 VGRLDSPSTRARQAAYEEVLQEIGEPYREEWILDGCIDLEDGAAIMDRLSGMEE---RPS 253 Query: 241 AMMISGDHIAAGLIIEARKHGLKIPEDLAVMGFDNQPIGRLLGITTIDNQLYQMGEAAFG 300 A++++GDH+AAGLI EA K GL IP++LAV+GFDNQPIG++LG+TTIDN+L+ MG AF Sbjct: 254 AILVTGDHVAAGLIYEAEKRGLSIPQELAVIGFDNQPIGKMLGLTTIDNRLFDMGTEAFR 313 Query: 301 IIHEQINGSSL-PVTRKLDYRIIERSTV 327 I++ I G P+ ++L + +IERS+V Sbjct: 314 ILYAHIQGEQKEPIRKELAFELIERSSV 341 >C3B7Q7.1 C3B7Q7_BACMY Transcriptional regulator, LacI Length = 332 Score = 317 bits (813), Expect = 8e-85, Method: Compositional matrix adjust. Identities = 152/328 (46%), Positives = 215/328 (65%), Gaps = 4/328 (1%) Query: 1 MANIKEIARIAGVSVTTVSRVLNNHPYVSKDKRTAVLQTIEQLNYTRNMNAVHLITGRTG 60 MANIK+IA+ AGVS+TTVSRVLN+HPYVS +KR VL +E+LNY +N+NA+HLITG+T Sbjct: 8 MANIKQIAKTAGVSITTVSRVLNDHPYVSDEKRKRVLDAVEELNYAKNINAIHLITGKTL 67 Query: 61 AVAVILPYISSFYFSVVMDGXXXXXXXXXYRLILCQTNYVPEEELKVLDMLRNKEIDGVI 120 V V+LP+I+ YFS +++G Y++ LCQTNY +EE++VL+M++ K+ DG+I Sbjct: 68 TVGVMLPFINLPYFSTIIEGIGNEALAAGYQINLCQTNYDSKEEIRVLEMMKMKQFDGMI 127 Query: 121 IVSTALKPEVIESYTIYGPVVTCQNSGERQFSSVYIEHYAAFRHGLEYLYGKGHRRIGYC 180 I S A E IE + + P+++C+ SSVY+ HY FR G EYL KGH +IG+C Sbjct: 128 ICSRASSWEQIEPFAKFAPIISCEKMEHPLVSSVYVNHYEGFRIGTEYLLRKGHEKIGFC 187 Query: 181 EGRQNGSSASIRQNAFQDFLAEKELLFDPEWMIFDCMREEDGAEVARRLLDNPDRSGRPE 240 RQN + R+ AF D L + P+WM + C +DGA+V +L+ + RP Sbjct: 188 LARQNSINTKQREKAFSDALHTAQKRVQPDWMFYQCYNMQDGAKVLHSILNMKE---RPT 244 Query: 241 AMMISGDHIAAGLIIEARKHGLKIPEDLAVMGFDNQPIGRLLGITTIDNQLYQMGEAAFG 300 A+ + D +AAGL EARKHGL++PEDLA++GFDN I + L ITTI++ MG AF Sbjct: 245 AIFAANDQVAAGLTTEARKHGLRVPEDLAILGFDNHEISKALEITTIEHPGLTMGARAFS 304 Query: 301 IIHEQINGSS-LPVTRKLDYRIIERSTV 327 + H+QI + + +L + +IER TV Sbjct: 305 LFHKQIQAEQIIGSSEELSFHLIERKTV 332 >C3AQH5.1 C3AQH5_BACMY Transcriptional regulator, LacI Length = 332 Score = 317 bits (813), Expect = 8e-85, Method: Compositional matrix adjust. Identities = 152/328 (46%), Positives = 215/328 (65%), Gaps = 4/328 (1%) Query: 1 MANIKEIARIAGVSVTTVSRVLNNHPYVSKDKRTAVLQTIEQLNYTRNMNAVHLITGRTG 60 MANIK+IA+ AGVS+TTVSRVLN+HPYVS +KR VL +E+LNY +N+NA+HLITG+T Sbjct: 8 MANIKQIAKTAGVSITTVSRVLNDHPYVSDEKRKRVLDAVEELNYAKNINAIHLITGKTL 67 Query: 61 AVAVILPYISSFYFSVVMDGXXXXXXXXXYRLILCQTNYVPEEELKVLDMLRNKEIDGVI 120 V V+LP+I+ YFS +++G Y++ LCQTNY +EE++VL+M++ K+ DG+I Sbjct: 68 TVGVMLPFINLPYFSTIIEGIGNEALAAGYQINLCQTNYDSKEEIRVLEMMKMKQFDGMI 127 Query: 121 IVSTALKPEVIESYTIYGPVVTCQNSGERQFSSVYIEHYAAFRHGLEYLYGKGHRRIGYC 180 I S A E IE + + P+++C+ SSVY+ HY FR G EYL KGH +IG+C Sbjct: 128 ICSRASSWEQIEPFAKFAPIISCEKMEHPLVSSVYVNHYEGFRIGTEYLLRKGHEKIGFC 187 Query: 181 EGRQNGSSASIRQNAFQDFLAEKELLFDPEWMIFDCMREEDGAEVARRLLDNPDRSGRPE 240 RQN + R+ AF D L + P+WM + C +DGA+V +L+ + RP Sbjct: 188 LARQNSINTKQREKAFSDALHTAQKRVQPDWMFYQCYNMQDGAKVLHSILNMKE---RPT 244 Query: 241 AMMISGDHIAAGLIIEARKHGLKIPEDLAVMGFDNQPIGRLLGITTIDNQLYQMGEAAFG 300 A+ + D +AAGL EARKHGL++PEDLA++GFDN I + L ITTI++ MG AF Sbjct: 245 AIFAANDQVAAGLTTEARKHGLRVPEDLAILGFDNHEISKALEITTIEHPGLTMGARAFS 304 Query: 301 IIHEQINGSS-LPVTRKLDYRIIERSTV 327 + H+QI + + +L + +IER TV Sbjct: 305 LFHKQIQAEQIIGSSEELSFHLIERKTV 332 >C3BPV4.1 C3BPV4_9BACI Transcriptional regulator, LacI Length = 332 Score = 315 bits (807), Expect = 4e-84, Method: Compositional matrix adjust. Identities = 152/328 (46%), Positives = 214/328 (65%), Gaps = 4/328 (1%) Query: 1 MANIKEIARIAGVSVTTVSRVLNNHPYVSKDKRTAVLQTIEQLNYTRNMNAVHLITGRTG 60 MANIK+IA+ AGVS+TTVSRVLN+HPYVS +KR VL +E+LNY +N+NA+HLITG+T Sbjct: 8 MANIKQIAKTAGVSITTVSRVLNDHPYVSDEKRKRVLDAVEELNYAKNINAIHLITGKTL 67 Query: 61 AVAVILPYISSFYFSVVMDGXXXXXXXXXYRLILCQTNYVPEEELKVLDMLRNKEIDGVI 120 V V+LP+I+ YFS +++G Y++ LCQTNY +EE++VL+M++ K+ DG+I Sbjct: 68 TVGVMLPFINLPYFSTIIEGIGNEALAAGYQINLCQTNYDSKEEIRVLEMMKMKQFDGMI 127 Query: 121 IVSTALKPEVIESYTIYGPVVTCQNSGERQFSSVYIEHYAAFRHGLEYLYGKGHRRIGYC 180 I S A E IE + + P+++C+ SSVY+ HY FR G EYL KGH +IG+C Sbjct: 128 ICSRASSWEQIEPFAKFAPIISCEKMEHPLVSSVYVNHYEGFRIGTEYLLRKGHEKIGFC 187 Query: 181 EGRQNGSSASIRQNAFQDFLAEKELLFDPEWMIFDCMREEDGAEVARRLLDNPDRSGRPE 240 RQN + R+ AF D L + P+WM + C +DGA+V +L+ + RP Sbjct: 188 LARQNSINTKQREKAFSDALHTAQKRVQPDWMFYQCYNMQDGAKVLHSILNMKE---RPT 244 Query: 241 AMMISGDHIAAGLIIEARKHGLKIPEDLAVMGFDNQPIGRLLGITTIDNQLYQMGEAAFG 300 A+ + D +AAGL EARKHGL++PEDLA++GFDN I L ITTI++ MG AF Sbjct: 245 AIFAANDQVAAGLTTEARKHGLRVPEDLAILGFDNHEISTALEITTIEHPGLTMGTRAFS 304 Query: 301 IIHEQINGSS-LPVTRKLDYRIIERSTV 327 + H+QI + + +L + +IER TV Sbjct: 305 LFHKQIQAEQIIGSSEELPFHLIERKTV 332 >Q81IW5.1 Q81IW5_BACCR Transcriptional regulator, LacI family Length = 325 Score = 315 bits (806), Expect = 5e-84, Method: Compositional matrix adjust. Identities = 151/328 (46%), Positives = 225/328 (68%), Gaps = 4/328 (1%) Query: 1 MANIKEIARIAGVSVTTVSRVLNNHPYVSKDKRTAVLQTIEQLNYTRNMNAVHLITGRTG 60 MANIK+IA++AGVSVTTVSRVLN+HPYVS++KR AV++ +E+LNY++N NAVHL G+T Sbjct: 1 MANIKDIAKMAGVSVTTVSRVLNDHPYVSEEKRKAVIEIVEKLNYSQNANAVHLSKGKTN 60 Query: 61 AVAVILPYISSFYFSVVMDGXXXXXXXXXYRLILCQTNYVPEEELKVLDMLRNKEIDGVI 120 V VILPYI+ F ++ G YR++LCQTNY +EE+K L ML+ K++DG+I Sbjct: 61 IVGVILPYINHPCFDAMVSGMMETALAHNYRVLLCQTNYNKKEEMKSLHMLKTKQLDGLI 120 Query: 121 IVSTALKPEVIESYTIYGPVVTCQNSGERQFSSVYIEHYAAFRHGLEYLYGKGHRRIGYC 180 I S A E IE Y YG ++ C+++ SSVY H AAF+ G++YL KG+++IGYC Sbjct: 121 ICSRANDWETIEPYASYGAIIACEDNDISNISSVYTNHSAAFQLGMDYLIEKGYKKIGYC 180 Query: 181 EGRQNGSSASIRQNAFQDFLAEKELLFDPEWMIFDCMREEDGAEVARRLLDNPDRSGRPE 240 GR+ G S+ R + ++ L + + EW+ +C EDG +VA +L + D PE Sbjct: 181 TGRKLGPSSQKRFDVYKQQLQSIDEEVNEEWIFTECFTLEDGVKVAHKLKEMQD---LPE 237 Query: 241 AMMISGDHIAAGLIIEARKHGLKIPEDLAVMGFDNQPIGRLLGITTIDNQLYQMGEAAFG 300 A++++GD +A G++ E K G+++PEDLA++GFDNQPI ++L +TTID L ++G+ AF Sbjct: 238 ALIVAGDEVAIGVMTEVEKLGIQVPEDLAIIGFDNQPISQVLQLTTIDQNLKEIGKTAFE 297 Query: 301 IIHEQINGSSLPVTR-KLDYRIIERSTV 327 ++H ++N S + ++ Y ++ERSTV Sbjct: 298 MLHRKVNDESSEQEKLEIPYELVERSTV 325 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.321 0.138 0.397 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,827,804,876 Number of extensions: 74231517 Number of successful extensions: 193266 Number of sequences better than 1.0e-05: 7887 Number of HSP's gapped: 181861 Number of HSP's successfully gapped: 7893 Length of query: 327 Length of database: 3,060,696,203 Length adjustment: 136 Effective length of query: 191 Effective length of database: 1,779,438,163 Effective search space: 339872689133 Effective search space used: 339872689133 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)