BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0337.0015.p (473 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1DKP9.1 B1DKP9_9BACL GerA spore germination protein 464 e-129 B3KI64.1 B3KI64_9BACI GerA spore germination protein 446 e-123 C3C4B9.1 C3C4B9_BACTU GerA spore germination protein 268 6e-70 C2PH48.1 C2PH48_BACCE GerA spore germination protein 266 3e-69 C2VDY0.1 C2VDY0_BACCE GerA spore germination protein 259 4e-67 >B1DKP9.1 B1DKP9_9BACL GerA spore germination protein Length = 512 Score = 464 bits (1195), Expect = e-129, Method: Compositional matrix adjust. Identities = 230/472 (48%), Positives = 301/472 (63%), Gaps = 4/472 (0%) Query: 2 DANPQSASMLRHVLEEFDQCADLIFRSFPEIRTDIVYFPSIVDQKMFTDEILAPFAQVQL 61 D+ P + L H L+EF +C+D+ R+FP+ + D+VYF + D+ + IL+P Sbjct: 44 DSQPPT---LAHTLDEFKECSDITHRTFPDYQVDLVYFDQLTDKDKLSRHILSPLLSGSG 100 Query: 62 DEVEQLFQQSQFTRVTDSKTVIEGILAGSVAIFHDKNIYLLDIYGPKTRTVQQSETETII 121 +E+ + + Q + T SKT+I +L+G +A+F+ + Y+ D++ P++R + QSETET+I Sbjct: 101 EEIAMMLKHLQSFKTTSSKTLILDVLSGGIAVFNGPDAYVFDVFSPESRAISQSETETVI 160 Query: 122 AGPHDAFVESAMTNISLIRQRIKSSHLKIKEYSVGEITKTSVYVLYIKDIANMKYVGELE 181 GPHDAF E T++++IR R+KSSHLK+ + SVGEITKT VY+LY+KDI NMK+V E + Sbjct: 161 TGPHDAFTEQINTSLAMIRARLKSSHLKVIKLSVGEITKTDVYLLYVKDIVNMKFVEEAK 220 Query: 182 KRIKRIEIDAVNDGGMLTQFLEDRPNSVFPQFLTTERPDVAVSKLTAGRIITLIDNXXXX 241 RI++IE+DA+ D ML Q+++D S+FPQ+LT+ERPD VSKL AGR+ L+D Sbjct: 221 TRIQKIEVDAILDTNMLIQYMDDNKYSIFPQYLTSERPDAIVSKLVAGRVAGLVDGSPSA 280 Query: 242 XXXXXXXXXXXXXXDDYYQRWAXXXXXXXXXXXXXXXXXSFTAFYVSVTTFHYEMIPEAL 301 DDYYQRW SFTA YVSVTTFHYEMIPE L Sbjct: 281 LSLPTSFFEFFSSSDDYYQRWILGSAIRLLRFIAFLITISFTALYVSVTTFHYEMIPENL 340 Query: 302 LRTLAESRNRVPFPPVYEALLMETMIELLREAGARLPTKXXXXXXXXXXXXXXXXXXXXX 361 L TL ESR RVPFPP+YEALLME IELLREAGARLPTK Sbjct: 341 LMTLTESRTRVPFPPLYEALLMEVTIELLREAGARLPTKIGQTIGIVGGIVIGQAAVQAG 400 Query: 362 LTSNVLXXXXXXXXXXXXXXXXYMMSASIRLVRFGIIMLAGIWGNMGISMGVALIVTHIS 421 LTSN+L Y+MSASIRL+RFG+I+L G GN G+ +G+A IV H+S Sbjct: 401 LTSNILIIAVASSAIASFVIPSYVMSASIRLIRFGLILLTGFLGNFGLVVGIAAIVIHLS 460 Query: 422 SLTSLGSSYLIPVAPMALGDWKDNFIRAPFTFLTKRPSQTQSPNPIRQNKRK 473 LTS+GSSYL PVAP DWKD FIRAPF +L +RPSQ+ SPN +R NK K Sbjct: 461 GLTSMGSSYLNPVAPFEPKDWKDIFIRAPFQWLRERPSQSASPNMVR-NKTK 511 >B3KI64.1 B3KI64_9BACI GerA spore germination protein Length = 501 Score = 446 bits (1147), Expect = e-123, Method: Compositional matrix adjust. Identities = 218/474 (45%), Positives = 304/474 (64%), Gaps = 3/474 (0%) Query: 2 DANPQS--ASMLRHVLEEFDQCADLIFRSFPEIRTDIVYFPSIVDQKMFTDEILAPFAQV 59 D PQ + L+HV ++FD AD + + + R ++YF +V++ ++L P Sbjct: 29 DPTPQEEVSLTLKHVADQFDDSADFVHKIYLHGRLHVMYFSHLVNEDRLERDLLPPILNS 88 Query: 60 QLDEVEQLFQQSQFTRVTDSKTVIEGILAGSVAIFHDKNIYLLDIYGPKTRTVQQSETET 119 + ++ L +QSQ+ VTDSKT +EGIL+G A +++ +++D+ P +RTV +SETE+ Sbjct: 89 D-NPLDLLTEQSQYEHVTDSKTYVEGILSGMAAFYYEDQAFMIDVIFPTSRTVAESETES 147 Query: 120 IIAGPHDAFVESAMTNISLIRQRIKSSHLKIKEYSVGEITKTSVYVLYIKDIANMKYVGE 179 II GPH+AF E A TN+SLIR+R++SSHLK+ + SVGE+TK+ +YVLYI+DIAN KYV E Sbjct: 148 IIIGPHEAFTEQAYTNLSLIRKRVQSSHLKVIKLSVGEVTKSDIYVLYIQDIANEKYVKE 207 Query: 180 LEKRIKRIEIDAVNDGGMLTQFLEDRPNSVFPQFLTTERPDVAVSKLTAGRIITLIDNXX 239 + KR++ IEIDA++D ML Q ++D P S+F QFLT+ERPD SKL +GR++ ++D Sbjct: 208 VIKRVEAIEIDAIHDTNMLIQCIDDDPYSIFAQFLTSERPDTIASKLVSGRVVGIMDGSP 267 Query: 240 XXXXXXXXXXXXXXXXDDYYQRWAXXXXXXXXXXXXXXXXXSFTAFYVSVTTFHYEMIPE 299 DDYYQRW+ + TA YVSVT+FHYEMIPE Sbjct: 268 TAFSAPANFFEFFSSSDDYYQRWSIGTATLFLRFAALLITVTLTALYVSVTSFHYEMIPE 327 Query: 300 ALLRTLAESRNRVPFPPVYEALLMETMIELLREAGARLPTKXXXXXXXXXXXXXXXXXXX 359 L+ TL ESR++VPFPP++EALLMET IELLREAGARLPTK Sbjct: 328 NLILTLTESRSKVPFPPIFEALLMETTIELLREAGARLPTKIGQTIGIVGGIVIGQAAVQ 387 Query: 360 XXLTSNVLXXXXXXXXXXXXXXXXYMMSASIRLVRFGIIMLAGIWGNMGISMGVALIVTH 419 LTSN+L Y+MSAS RL RFG+I+LAG+WGNMG+++G+A +V H Sbjct: 388 AGLTSNILIISVASSAIASFVIPNYVMSASFRLFRFGLIVLAGLWGNMGLALGLAYMVIH 447 Query: 420 ISSLTSLGSSYLIPVAPMALGDWKDNFIRAPFTFLTKRPSQTQSPNPIRQNKRK 473 +S LTS+GSSYL P+AP DWKD F+R PF+ L KRP+Q ++ N ++ ++ Sbjct: 448 LSGLTSVGSSYLTPIAPFEPYDWKDVFLRLPFSTLAKRPTQVKAKNKVKLKMKR 501 >C3C4B9.1 C3C4B9_BACTU GerA spore germination protein Length = 515 Score = 268 bits (686), Expect = 6e-70, Method: Compositional matrix adjust. Identities = 153/468 (32%), Positives = 235/468 (50%), Gaps = 7/468 (1%) Query: 13 HVLEEFDQCADLIFRSFP----EIRTDIVYFPSIVDQKMFTDEILAPFAQVQLDEVEQLF 68 H+ F Q DL+ R F + +VY + D+++ + +L+P D +L Sbjct: 45 HIQNIFRQAPDLVIRQFQITVGGLEAALVYLSGLTDKELIQNHVLSPLMNRDFDTNTELP 104 Query: 69 QQSQFTRVTDSKTVIEGILAG--SVAIFHDKNI-YLLDIYGPKTRTVQQSETETIIAGPH 125 ++ ++ + IE + G SV + H + + LD G R+V+ + E + G H Sbjct: 105 IPLGQIKIINTLSQIENAIYGGDSVLLIHSRTTAHQLDTKGWPQRSVEDAHNEIALKGAH 164 Query: 126 DAFVESAMTNISLIRQRIKSSHLKIKEYSVGEITKTSVYVLYIKDIANMKYVGELEKRIK 185 AF+E+ NI++IR+ I L IKE +G +KT + +LY+KD+A+ + ELE RI+ Sbjct: 165 QAFIETGSQNIAMIRRYIPHRELAIKEIVIGVRSKTKISILYLKDVASEDVLKELETRIQ 224 Query: 186 RIEIDAVNDGGMLTQFLEDRPNSVFPQFLTTERPDVAVSKLTAGRIITLIDNXXXXXXXX 245 I IDAV G L +F+ED P S FPQF+ TERPD+AVS + GR +T++D+ Sbjct: 225 NITIDAVTSNGELIEFIEDHPYSPFPQFILTERPDLAVSHILQGRFVTVMDHSPNVLITP 284 Query: 246 XXXXXXXXXXDDYYQRWAXXXXXXXXXXXXXXXXXSFTAFYVSVTTFHYEMIPEALLRTL 305 DDY RW A YV+ +F++E+IP L ++ Sbjct: 285 TNFISFFQGVDDYGTRWLVASSIRLLRFVAFMVALFLPASYVAFISFNFEVIPVELYLSI 344 Query: 306 AESRNRVPFPPVYEALLMETMIELLREAGARLPTKXXXXXXXXXXXXXXXXXXXXXLTSN 365 AESR RVPF PV EALLME +E +RE R+PT + SN Sbjct: 345 AESRTRVPFSPVMEALLMEITLETMREGALRIPTPIGQTVGIVGGIVIGQAAVQAGIVSN 404 Query: 366 VLXXXXXXXXXXXXXXXXYMMSASIRLVRFGIIMLAGIWGNMGISMGVALIVTHISSLTS 425 ++ Y M A++RL+RF +++ A ++G +G+ +G ++ H+SSL S Sbjct: 405 IMIIVVAVTAISSFVIPNYDMGAAVRLLRFPMMLAAALFGIVGLVIGWMTLIGHLSSLKS 464 Query: 426 LGSSYLIPVAPMALGDWKDNFIRAPFTFLTKRPSQTQSPNPIRQNKRK 473 LG+SY P+AP D KD F+R P + +RP + IRQ K + Sbjct: 465 LGTSYGTPLAPFRFADMKDVFVRFPLWTIRRRPKGNGTNQTIRQGKNR 512 >C2PH48.1 C2PH48_BACCE GerA spore germination protein Length = 515 Score = 266 bits (681), Expect = 3e-69, Method: Compositional matrix adjust. Identities = 154/464 (33%), Positives = 235/464 (50%), Gaps = 9/464 (1%) Query: 18 FDQCADLIFRSFPEIRTD-----IVYFPSIVDQKMFTDEILAPFAQVQLDEVEQLFQQSQ 72 F Q DL+ R F +I D +VY + D+++ + +L+P D +L Sbjct: 50 FRQAPDLVIRRF-QITVDGLEAALVYLSGLTDKELIQNHVLSPLMNSDFDTNTELPIPLG 108 Query: 73 FTRVTDSKTVIEGILAG--SVAIFHDKNI-YLLDIYGPKTRTVQQSETETIIAGPHDAFV 129 ++ ++ + IE + G SV + H + + LD G R+V+ + E + G H AF+ Sbjct: 109 QIKIINTLSQIENAIYGGDSVLLIHSRTTAHQLDTKGWPQRSVEDAHNEIALKGAHQAFI 168 Query: 130 ESAMTNISLIRQRIKSSHLKIKEYSVGEITKTSVYVLYIKDIANMKYVGELEKRIKRIEI 189 E+ NI++IR+ I L IKE +G +KT + +LY+KD+A+ + ELE RI+ I I Sbjct: 169 ETGSQNIAMIRRYIPHRELAIKEIVIGVRSKTQISILYLKDVASEDVLKELETRIQNITI 228 Query: 190 DAVNDGGMLTQFLEDRPNSVFPQFLTTERPDVAVSKLTAGRIITLIDNXXXXXXXXXXXX 249 DAV G L +F+ED P S FPQF+ TERPD+AVS + GR +T++D+ Sbjct: 229 DAVTSNGELIEFIEDHPYSPFPQFILTERPDLAVSHILQGRFVTVMDHSPNVLITPTNFI 288 Query: 250 XXXXXXDDYYQRWAXXXXXXXXXXXXXXXXXSFTAFYVSVTTFHYEMIPEALLRTLAESR 309 DDY RW A YV+ +F++E+IP L ++AESR Sbjct: 289 SFFQGVDDYGTRWLVASSIRLLRFIAFMVALFLPASYVAFISFNFEVIPVELYLSIAESR 348 Query: 310 NRVPFPPVYEALLMETMIELLREAGARLPTKXXXXXXXXXXXXXXXXXXXXXLTSNVLXX 369 RVPF PV EALLME +E +RE R+PT + SN++ Sbjct: 349 TRVPFSPVMEALLMEITLETMREGALRIPTPIGQTVGIVGGIVIGQAAVQAGIVSNIMII 408 Query: 370 XXXXXXXXXXXXXXYMMSASIRLVRFGIIMLAGIWGNMGISMGVALIVTHISSLTSLGSS 429 Y M A++RL+RF +++ A ++G +G+ +G ++ H+SSL SLG+S Sbjct: 409 VVAVTAISSFVIPNYDMGAAVRLLRFPMMLAAALFGIVGLVIGWMTLIGHLSSLKSLGTS 468 Query: 430 YLIPVAPMALGDWKDNFIRAPFTFLTKRPSQTQSPNPIRQNKRK 473 Y P+AP D KD F+R P + +RP + IRQ K + Sbjct: 469 YGTPLAPFRFADMKDVFVRFPLWTIRRRPKGNGTIQSIRQGKNR 512 >C2VDY0.1 C2VDY0_BACCE GerA spore germination protein Length = 515 Score = 259 bits (662), Expect = 4e-67, Method: Compositional matrix adjust. Identities = 149/462 (32%), Positives = 234/462 (50%), Gaps = 9/462 (1%) Query: 18 FDQCADLIFRSFPEIRTD-----IVYFPSIVDQKMFTDEILAPFAQVQLDEVEQLFQQSQ 72 F Q DL+ R F +I D +VY + D+++ + +L+P + +L Sbjct: 50 FRQAPDLVIRRF-QITVDGLEAALVYLSGLTDKELIQNHVLSPLMNSDFNTNTELPIPLG 108 Query: 73 FTRVTDSKTVIEGILAG--SVAIFHDKNI-YLLDIYGPKTRTVQQSETETIIAGPHDAFV 129 ++ ++ + IE + G SV + H + + LD G R+V+ + E + G H AF+ Sbjct: 109 QIKIINTLSQIENAIFGGDSVLLIHSRTTAHQLDTKGWPQRSVEDAHNEIALKGAHQAFI 168 Query: 130 ESAMTNISLIRQRIKSSHLKIKEYSVGEITKTSVYVLYIKDIANMKYVGELEKRIKRIEI 189 E+ NI++IR+ I L IKE ++G +KT + +LY+KD+A+ + ELE RI+ I + Sbjct: 169 ETGSQNIAMIRRYIPYRELTIKEMAIGVRSKTRISILYLKDVASENVLKELETRIQNITV 228 Query: 190 DAVNDGGMLTQFLEDRPNSVFPQFLTTERPDVAVSKLTAGRIITLIDNXXXXXXXXXXXX 249 DAV G L +F+ED P S FPQF+ TERPD+AVS + GR +T++D+ Sbjct: 229 DAVASNGELIEFIEDNPYSPFPQFILTERPDLAVSHILQGRFVTVMDHSPNVLITPTNFI 288 Query: 250 XXXXXXDDYYQRWAXXXXXXXXXXXXXXXXXSFTAFYVSVTTFHYEMIPEALLRTLAESR 309 DDY RW A YV+ +F++E+IP L ++AESR Sbjct: 289 SFFQGVDDYGTRWLVASSIRVLRFIAFMIALFLPASYVAFISFNFEVIPVELYLSIAESR 348 Query: 310 NRVPFPPVYEALLMETMIELLREAGARLPTKXXXXXXXXXXXXXXXXXXXXXLTSNVLXX 369 RVPF PV EALLME +E +RE R+PT + SN++ Sbjct: 349 TRVPFSPVMEALLMEITLETMREGALRIPTPIGQTVGIVGGIVIGQAAVQAGIVSNIMII 408 Query: 370 XXXXXXXXXXXXXXYMMSASIRLVRFGIIMLAGIWGNMGISMGVALIVTHISSLTSLGSS 429 Y M A++R +RF +++ A ++G +G+ +G ++ H+ SL SLG+ Sbjct: 409 VVAVTAISSFVIPNYDMGAAVRFLRFPMMLAAALFGIVGLVIGWMTLIGHLISLESLGTP 468 Query: 430 YLIPVAPMALGDWKDNFIRAPFTFLTKRPSQTQSPNPIRQNK 471 Y +P+AP D KD F+R P + +RP ++ IRQ K Sbjct: 469 YGMPLAPFRFADMKDAFVRFPLWAIRRRPKGKRAIQSIRQGK 510 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.322 0.136 0.387 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,134,462,492 Number of extensions: 73741041 Number of successful extensions: 195292 Number of sequences better than 1.0e-05: 1080 Number of HSP's gapped: 193055 Number of HSP's successfully gapped: 1101 Length of query: 473 Length of database: 3,060,696,203 Length adjustment: 140 Effective length of query: 333 Effective length of database: 1,741,754,103 Effective search space: 580004116299 Effective search space used: 580004116299 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 133 (55.8 bits)