BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0337.0014.p (359 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KI65.1 B3KI65_9BACI Spore germination protein 313 1e-83 B1DKP8.1 B1DKP8_9BACL Spore germination protein 303 1e-80 Q2B1X3.1 Q2B1X3_9BACI Spore germination protein 84 2e-14 C2YDH8.1 C2YDH8_BACCE Spore germination protein 83 5e-14 B7HAU7.1 B7HAU7_BACC4 Spore germination protein 82 1e-13 >B3KI65.1 B3KI65_9BACI Spore germination protein Length = 362 Score = 313 bits (803), Expect = 1e-83, Method: Compositional matrix adjust. Identities = 160/358 (44%), Positives = 209/358 (58%) Query: 1 MMEKLSPFHVAILVYMTQIGIVVFSLPRQLADYFGTNGWXXXXXXXXXTSFNIYLITLVY 60 M EKLS FH AIL+YM Q G+ VFS+ + A YFGTNGW NIY + VY Sbjct: 1 MKEKLSQFHTAILIYMIQTGVYVFSITQVEAQYFGTNGWLITIPASILVCLNIYQMYWVY 60 Query: 61 RLGDGKPVFEIMERSIPKVILYPVYLGLCAVWLIFGCMIGKKYVLLFQMIAFPTTNPMXX 120 RLG+G+ +F I+E+SIPK IL P YL + VW + GC++ K+YVL+FQM AFPTTNPM Sbjct: 61 RLGEGQSIFVILEKSIPKFILAPFYLAISTVWALIGCLVAKQYVLIFQMFAFPTTNPMVF 120 Query: 121 XXXXXXXXXXXXIKGIFTISKAATIFFWLTFWMYFLLLWFFPSFRWVRLTPFILQGGHDQ 180 KG++ ISKA+T+FFW T WM LLL++ FR RLTP+I + + Sbjct: 121 KIAIDVLAFMLLTKGLYNISKASTVFFWCTIWMSLLLLYYAGEFRIERLTPYIFKDSSNM 180 Query: 181 IKGAFDLYTVYLGYELSILLFPYMDKSKKSLAAIYAGNAYLSVIYISLSIVCFGFFSFGQ 240 ++G F +Y YLGYEL +LLFPY DK K A+ GN SV Y L V FG Sbjct: 181 LEGIFQIYLAYLGYELCLLLFPYTDKKTKLFRAVIYGNLIRSVSYTILGFVSFGVVGSDM 240 Query: 241 XXXXXXXXXXXXGYIQFPFVERLENMLYGFFLFLILITAAMYWWGAMEAMRRIIPKLKIP 300 YI+ PF+ER+EN+ YGFFLF LIT +Y+W A E+ +R+ PK+K+ Sbjct: 241 LKVMLFPLLDLLAYIKLPFIERIENLFYGFFLFTTLITLVLYFWAAGESTQRVFPKIKMN 300 Query: 301 VMVFLLTAIAYCISYIPKTLDQVNNWILHLGYAAIGIAFGXXXXXXXXXXXQGRRVSR 358 V F + +A ISYIP L+++ W+L LGY IG A+ Q R+ R Sbjct: 301 VQYFFIILVALLISYIPAVLNRIREWLLFLGYIQIGFAYIMPLILIMLLLWQRRKRQR 358 >B1DKP8.1 B1DKP8_9BACL Spore germination protein Length = 369 Score = 303 bits (777), Expect = 1e-80, Method: Compositional matrix adjust. Identities = 152/338 (44%), Positives = 207/338 (61%) Query: 3 EKLSPFHVAILVYMTQIGIVVFSLPRQLADYFGTNGWXXXXXXXXXTSFNIYLITLVYRL 62 EKLSPFH ILVYM Q G++ LP+ LA+ FGTNGW + NI LI VY+L Sbjct: 7 EKLSPFHTTILVYMIQTGVMALILPQLLAETFGTNGWITVLGFGLIAAVNILLIGAVYKL 66 Query: 63 GDGKPVFEIMERSIPKVILYPVYLGLCAVWLIFGCMIGKKYVLLFQMIAFPTTNPMXXXX 122 G G+ +F+I+E+SIPK +L PVYL + + GCM GK+Y+L+FQM AFPTTNPM Sbjct: 67 GKGRSIFQILEQSIPKFLLVPVYLAIALNCAMLGCMAGKEYILIFQMYAFPTTNPMEFKL 126 Query: 123 XXXXXXXXXXIKGIFTISKAATIFFWLTFWMYFLLLWFFPSFRWVRLTPFILQGGHDQIK 182 KG++ ISKA+T+FFWL WM+ LL +F+ F W RLTPF+ +GGH Sbjct: 127 IMDALVFWLLTKGLYNISKASTVFFWLFAWMFLLLFFFYSDFNWSRLTPFLFKGGHFSYL 186 Query: 183 GAFDLYTVYLGYELSILLFPYMDKSKKSLAAIYAGNAYLSVIYISLSIVCFGFFSFGQXX 242 + D+Y+ +LGYE+ +L F YM++ K + + GN + Y LSIV FG +S Q Sbjct: 187 ESLDIYSSFLGYEVLLLAFGYMNQRTKFVKSALIGNGMTTFTYFYLSIVTFGVYSLEQLK 246 Query: 243 XXXXXXXXXXGYIQFPFVERLENMLYGFFLFLILITAAMYWWGAMEAMRRIIPKLKIPVM 302 YI+FPF+ER+EN+LYGF LF L + MY W A+E +++ PKLK + Sbjct: 247 NKAFPVLEMLAYIRFPFIERMENLLYGFILFTALFSIVMYEWCALEMVKQSFPKLKGNFL 306 Query: 303 VFLLTAIAYCISYIPKTLDQVNNWILHLGYAAIGIAFG 340 F+L Y I++IP TL++V +W+ L GIAFG Sbjct: 307 AFILVMTCYLIAFIPDTLNEVGDWLSTLSLIECGIAFG 344 >Q2B1X3.1 Q2B1X3_9BACI Spore germination protein Length = 378 Score = 84.0 bits (206), Expect = 2e-14, Method: Compositional matrix adjust. Identities = 72/335 (21%), Positives = 145/335 (43%), Gaps = 14/335 (4%) Query: 13 LVYMTQIGIVVFSLPRQLADYFGTNGWXXXXXXXXXTSFNIYLITLVYRLGDGKPVFEIM 72 L++ Q G+ + LPR + G +GW S I+L ++ + + +F I Sbjct: 25 LIHTIQTGVGIAGLPRIVYLAAGHDGWISIIIAGLLVSAVIFLSVMMLKQYESADLFGIH 84 Query: 73 ERSIPKVILYPVYLG-----LCAVWLIFGC-MIGKKYVLLFQMIAFPTTNPMXXXXXXXX 126 ++ ++LG L +LI G +I YV + Q FP + Sbjct: 85 YD------VFGIFLGRLVNILYTFYLISGIYVIVMNYVEIVQAWVFPDLSTWFLAMILLL 138 Query: 127 XXXXXXIKGIFTISKAATIFFWLTFWMYFLLLWFFPSFRWVRLTPFILQGGHDQIKGAFD 186 + G+ + + F LTFW+ +++ F + L P + +G + +KG + Sbjct: 139 LSMYAVMGGVRIAVGFSFMSFLLTFWIVLIIIIPARDFDYTHLLPILNKGYAEILKGVYK 198 Query: 187 LYTVYLGYELSILLFPYMDKSKKSLAAIYAGNAYLSVIYISLSIVCFGFFSFGQXXXXXX 246 +G+E+ + L+P++ K++ G Y +++++ ++++ GFF Sbjct: 199 TSLSIIGFEMLLFLYPFIKNKKRAHLYTQLGGLYTTLLFVLVTVISIGFFGEKGLEKTIW 258 Query: 247 XXXXXXGYIQFPFVERLENMLYGFFLFLILITAAMYWWGAMEAMRRIIPKLKIPVMVFLL 306 ++ P +ER E + F++ +IL +Y W A M+R+ P +K + L+ Sbjct: 259 PVLSMFKIVRLPNLERFEFIAVSFWMLIILPNICLYLWAASRGMKRVFPGMKQKTFIVLI 318 Query: 307 TAIAYCISYIPKTLDQVNNWILHLGYAA--IGIAF 339 + + S++ K Q+N ++G A IGI + Sbjct: 319 AILVWSCSFLVKARYQMNIVSDYIGMAGFYIGICY 353 >C2YDH8.1 C2YDH8_BACCE Spore germination protein Length = 365 Score = 82.8 bits (203), Expect = 5e-14, Method: Compositional matrix adjust. Identities = 76/325 (23%), Positives = 130/325 (40%), Gaps = 14/325 (4%) Query: 3 EKLSPFHVAILVYMTQIGIVVFSLPRQLADYFGTNGWXXXXXXXXXTSFNIYLITLVYRL 62 ++S F + + Q+G V +LPR++A T+GW T+ I + Sbjct: 7 REISLFQYILTISGVQVGFGVLTLPREVAQGANTDGWMSIIIGCALTTLVSLCIVKIMEK 66 Query: 63 GDGKPVFEIMERSIPK------VILYPVYLGLCAVWLIFGCMIGKKYVLLFQMIAFPTTN 116 G + +++ R + K +I + +Y L A+ LIF + YV+ + P T Sbjct: 67 HPGYTLLDVLTRYLGKWLGRVGMIFWILYAVLAAISLIFSLL----YVI--HIWILPRTP 120 Query: 117 PMXXXXXXXXXXXXXXIKGIFTISKAATIFFWLTFWMYFLLLWFFPSFRWVRLTPFILQG 176 KG+ IS+ A + T WM FLL W+ L P + +G Sbjct: 121 MFLIMILLSIPMLMLACKGVLIISRYAVFTVFFTLWMPFLLFIPLKDGHWIYLLPLLKEG 180 Query: 177 GHDQIKGAFDLYTVYLGYELSILLFPYMDKSKKSLAAIYAGNAYLSVIYISLSIVCFGFF 236 + +LG+E + +L+PY+ + I N IY+ ++ V F +F Sbjct: 181 WIPVLNTVKSTIIAFLGFEFAFVLYPYLSNKSSAKKGIVLANLITLFIYLQVTFVSFVYF 240 Query: 237 SFGQXXXXXXXXXXXXGYIQFPFVERLENMLYGFFLFLILITAAMYWWGAMEAMRRIIPK 296 S F F+ER E + F+LF+I + Y + A + + + + K Sbjct: 241 SPDGITKFLWPTLSLVTPFHFSFLERFEIIFLSFYLFIIFDSCIPYIFTASDGINQFLNK 300 Query: 297 L--KIPVMVFLLTAIAYCISYIPKT 319 +P+ V L I YIP + Sbjct: 301 KGSSLPIYVLLFGCIFILFFYIPSS 325 >B7HAU7.1 B7HAU7_BACC4 Spore germination protein Length = 365 Score = 81.6 bits (200), Expect = 1e-13, Method: Compositional matrix adjust. Identities = 75/325 (23%), Positives = 131/325 (40%), Gaps = 14/325 (4%) Query: 3 EKLSPFHVAILVYMTQIGIVVFSLPRQLADYFGTNGWXXXXXXXXXTSFNIYLITLVYRL 62 ++S F + + Q+G V +LPR++A T+GW T+ I + Sbjct: 7 REISLFQYILTISGVQVGFGVLTLPREVAQGANTDGWMSIIIGCALTTLVSLCIVKIMEN 66 Query: 63 GDGKPVFEIMERSIPK------VILYPVYLGLCAVWLIFGCMIGKKYVLLFQMIAFPTTN 116 G + +++ R + + +I + +Y L A+ LIF + YV+ + P T Sbjct: 67 HPGYTLLDVLTRYLGEWLGRVGMIFWILYAVLAAISLIFSLL----YVI--HIWILPRTP 120 Query: 117 PMXXXXXXXXXXXXXXIKGIFTISKAATIFFWLTFWMYFLLLWFFPSFRWVRLTPFILQG 176 KG+ IS+ A + T WM FLL W+ L P + +G Sbjct: 121 MFLIMILLSIPMLMLACKGVLIISRYAVFTVFFTLWMPFLLFIPLKDGHWIYLLPLLKEG 180 Query: 177 GHDQIKGAFDLYTVYLGYELSILLFPYMDKSKKSLAAIYAGNAYLSVIYISLSIVCFGFF 236 + +LG+E + +L+PY+ + I N IY+ ++ V F +F Sbjct: 181 WIPVLNTVKSTIIAFLGFEFAFVLYPYLSNKSSAKKGIVLANLITLFIYLQVTFVSFVYF 240 Query: 237 SFGQXXXXXXXXXXXXGYIQFPFVERLENMLYGFFLFLILITAAMYWWGAMEAMRRIIPK 296 S F F+ER E + F+LF+I + Y + A + + +++ K Sbjct: 241 SPDGITKFLWPTLSLVTPFHFSFLERFEIIFLSFYLFIIFDSCIPYIFTASDGINQLLNK 300 Query: 297 L--KIPVMVFLLTAIAYCISYIPKT 319 +P+ V L I YIP + Sbjct: 301 KSSSLPIYVLLFGCIFILFFYIPSS 325 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.334 0.148 0.484 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,831,862,672 Number of extensions: 67687841 Number of successful extensions: 335698 Number of sequences better than 1.0e-05: 354 Number of HSP's gapped: 335066 Number of HSP's successfully gapped: 354 Length of query: 359 Length of database: 3,060,696,203 Length adjustment: 137 Effective length of query: 222 Effective length of database: 1,770,017,148 Effective search space: 392943806856 Effective search space used: 392943806856 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 39 (21.6 bits) S2: 132 (55.5 bits)