BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0337.0013.p (366 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1DKP7.1 B1DKP7_9BACL Germination protein, Ger(X)C family 339 3e-91 B3KI66.1 B3KI66_9BACI Germination protein, Ger(X)C family 331 6e-89 C0ZJY6.1 C0ZJY6_BREBN Spore germination protein 164 2e-38 Q9KAV0.1 Q9KAV0_BACHD BH2186 protein 154 2e-35 C0ZJ25.1 C0ZJ25_BREBN Spore germination protein 142 8e-32 >B1DKP7.1 B1DKP7_9BACL Germination protein, Ger(X)C family Length = 368 Score = 339 bits (869), Expect = 3e-91, Method: Compositional matrix adjust. Identities = 165/350 (47%), Positives = 244/350 (69%), Gaps = 4/350 (1%) Query: 17 GCNSDQRILEKLGMMQTASYDLAENDQLKATLSVPVIDPESSEHRHLLTAVTDSLKEARV 76 GC D RILE++G ASYD A DQ++ +SVP +P R + +AV S K+ARV Sbjct: 19 GC--DYRILERIGFTYAASYDTAPEDQVEIAISVPRAEPTIKLKREIHSAVGRSSKDARV 76 Query: 77 QLSNQIDLLLVSGQLRSVLFGLSLAKEGLADHVDTLIRDPSIALGVQVTVVDGDAGKLLS 136 + SN+ DLLLV+GQ+R +L+ +LA++ L++ +++++RD SI+ V++T+V G+A LL Sbjct: 77 KFSNETDLLLVNGQIRVLLYSDALARKDLSNEINSVLRDTSISPLVKITLVSGNAKDLLV 136 Query: 137 KTFKPNKDTGTYVNHLLKKESSSNIIPPTTLYEFTREYYDDGIDPIAPIVKDGGDRAGIS 196 K +K + T Y+N LL+K SS++ IP TTLYEF R+YYDDGIDP+AP++K+ G+ + Sbjct: 137 KDYKQHLSTDKYINRLLEKSSSNHRIPKTTLYEFERDYYDDGIDPVAPVLKEVGNDISVD 196 Query: 197 GIALFQDDRFVTKIPAKDGIIFALLRGNINQGELSVNLG--ERNGRKEMVMLSSMLNKRK 254 GI LFQD ++V KIP KD ++FA+LRGN +G++S++LG E G E +M SS+L+ RK Sbjct: 197 GIGLFQDYKYVAKIPNKDSLLFAILRGNFKKGDMSLDLGRSEEKGSLEFIMFSSVLSDRK 256 Query: 255 VSVQHLGDNRFKFIIANSIHGSVLEYTGHEDLSQSKDRHKLEADIAEYISDKAQEVVRQL 314 V V+H I I GSV EY G++ LS RH++E+ ++E +S +A +++QL Sbjct: 257 VHVEHKAGGTIHVSINGHIRGSVHEYIGNKKLSNDAQRHEVESKVSEILSARANRLIKQL 316 Query: 315 QQNNVDSIGIGQYVRNSLSYKAWKSLNWRKVYPGVEVECRVKVTIKDYGK 364 QQ+ VDS+GIG YVRNS+ Y+AWK L+WR+ YP VE++C++ KDYGK Sbjct: 317 QQHKVDSLGIGIYVRNSMKYEAWKKLDWREEYPKVEIDCKLHFKAKDYGK 366 >B3KI66.1 B3KI66_9BACI Germination protein, Ger(X)C family Length = 369 Score = 331 bits (849), Expect = 6e-89, Method: Compositional matrix adjust. Identities = 164/353 (46%), Positives = 244/353 (69%), Gaps = 5/353 (1%) Query: 16 TGCNSDQRILEKLGMMQTASYDLAENDQLKATLSVPVIDPESSEHRHLLTAVTDSLKEAR 75 GC DQRILE LG +QT SYDL ++ L ++S+P DPE++ R +LTA S KEA+ Sbjct: 18 AGCG-DQRILEDLGFIQTTSYDLLQDGNLAISVSIPQADPETASKREVLTATAKSSKEAK 76 Query: 76 VQLSNQIDLLLVSGQLRSVLFGLSLAKEGLADHVDTLIRDPSIALGVQVTVVDGDAGKLL 135 + +S Q +LLVSGQLR+VLFGLS+A++GL H+DTL RDPSI+ V+++VV+G+AG+LL Sbjct: 77 MIMSRQTGMLLVSGQLRNVLFGLSMAEQGLHGHLDTLYRDPSISSQVKISVVEGNAGELL 136 Query: 136 SKTFKPNKDTGTYVNHLLKKESSSNIIPPTTLYEFTREYYDDGIDPIAPIVKDGGDRAGI 195 +K + TG Y++ LL+KE+ IP TL+ F R+Y+D G+DP+API+K D+ I Sbjct: 137 IDDYKQHPRTGRYIDMLLEKEALGQTIPKVTLFNFARDYFDQGVDPVAPIIKK-HDKNYI 195 Query: 196 S--GIALFQDDRFVTKIPAKDGIIFALLRGNINQGELSVNLGER-NGRKEMVMLSSMLNK 252 + GIALF+DDR+V KI +D +IF+ L+G +G+L+++L E +KE+VM SS+++ Sbjct: 196 TTNGIALFKDDRYVAKIEPQDALIFSFLKGKFREGQLNIDLSEDPKDKKEVVMFSSLISS 255 Query: 253 RKVSVQHLGDNRFKFIIANSIHGSVLEYTGHEDLSQSKDRHKLEADIAEYISDKAQEVVR 312 RKV V K + + GSVLEY G LS ++RH+LE I++YI+ K ++ Sbjct: 256 RKVKVVRGDQGIDKVMFYVKVSGSVLEYIGDARLSNDRERHELEQRISQYITKKGNAMIS 315 Query: 313 QLQQNNVDSIGIGQYVRNSLSYKAWKSLNWRKVYPGVEVECRVKVTIKDYGKY 365 ++Q+ +DS+GIG+YVRN + Y+ WK ++WR+ ++VEC + V IKDYGK+ Sbjct: 316 IMKQHQIDSLGIGRYVRNRIGYEQWKKMDWRQELQKLQVECVIDVHIKDYGKF 368 >C0ZJY6.1 C0ZJY6_BREBN Spore germination protein Length = 377 Score = 164 bits (414), Expect = 2e-38, Method: Compositional matrix adjust. Identities = 106/370 (28%), Positives = 182/370 (49%), Gaps = 16/370 (4%) Query: 3 KRLSAVXXXXXXXTGCNSDQRILEKLGMMQTASYDLAENDQLKATLSVPVIDPESSEHRH 62 KRL A+ SD++I ++ G+ YDL ++ ++ T +P I+PE+ E Sbjct: 4 KRLLALWCSCILVLAGCSDRQIFDETGLATAVGYDLLKDGDVRGTAIMPSINPEAKEKIQ 63 Query: 63 LLTAVTDSLKEARVQLSNQIDLLLVSGQLRSVLFGLSLAKEGLADHVDTLIRDPSIALGV 122 + +A+ ++ K R +++ Q D ++ GQ+R L+ LAK GL VDTL RD SI+ V Sbjct: 64 VFSAIGETSKGIRDRINLQSDKRVLGGQIRVALYNEKLAKRGLGSIVDTLYRDSSISSRV 123 Query: 123 QVTVVDGDAGKLLSKTFKPNKDTGTYVNHLLKKESSSNIIPPTTLYEFTREYYDDGIDPI 182 + + +G+ +L+ + + G Y+ ++++ IP +T++EF R YYD GIDPI Sbjct: 124 YLCLFEGETYDMLTHPYSEEGNIGIYLYRMIEQNIEGEKIPSSTMHEFFRSYYDKGIDPI 183 Query: 183 APIVKDGGDRAGISGIALFQDDRFVTKIPAKDGIIFALLRGNIN------------QGEL 230 P + G+ I GIALF+ +R+V I K+ + L+ G GE+ Sbjct: 184 MPFIARKGNELQIKGIALFRGERYVGWITPKEAFLIKLMHGKFRIGSYDTSVPAHIFGEM 243 Query: 231 SVNLGERNGRKEMVMLSSMLNKRKVSVQHLGDNRFKFIIANSIHGSVLEYTGHEDLSQSK 290 N G + R ++V ++ + K+ + + F I ++ +LE T L ++ Sbjct: 244 PKNRGSKKDRVQLV-FDTISSHSKIELTQVSPPIFDIHI--RLNARILELTLPLKLENAR 300 Query: 291 DRHKLEADIAEYISDKAQEVVRQLQQNNVDSIGIGQYVRNSLSYKA-WKSLNWRKVYPGV 349 +E + E + + Q+VV +LQ NVD G G R++ KA WRK++ Sbjct: 301 LMKIMEHALEEELGKELQKVVHKLQDLNVDPAGFGIVYRSNRRGKANMTQAEWRKMFKEA 360 Query: 350 EVECRVKVTI 359 +K TI Sbjct: 361 TFRFHIKSTI 370 >Q9KAV0.1 Q9KAV0_BACHD BH2186 protein Length = 369 Score = 154 bits (388), Expect = 2e-35, Method: Compositional matrix adjust. Identities = 100/354 (28%), Positives = 178/354 (50%), Gaps = 8/354 (2%) Query: 16 TGCNSDQRILEKLGMMQTASYDLAENDQ--LKATLSVPVIDPESSEHRHLLTAVTDSLKE 73 TGC D+RILE+L M+ + D+AE D L + + P + E L++ SL+E Sbjct: 18 TGC-WDERILEELAMITILTVDVAEEDSPYLIVSATFPEFSKRAKEPETLISTKARSLRE 76 Query: 74 ARVQLSNQIDLLLVSGQLRSVLFGLSLAKEGLADHVDTLIRDPSIALGVQVTVVDGDAGK 133 A +L+ Q D +V GQ++ VL G A+ G+ +DTL RD ++ + V + +G A + Sbjct: 77 AMEKLNAQTDKKIVQGQIQVVLLGEEFARGGIQSTLDTLYRDVDVSAALYVCITEGRADE 136 Query: 134 LLSKTFKPNKDTGTYVNHLLKKESSSNIIPPTTLYEFTREYYDDGIDPIAPIVKDGGDRA 193 L+++ F+ G Y++ L++K+ N++P T L++F EY DP+ P V G + A Sbjct: 137 LIARAFQDKPMDGFYLSRLIQKQREVNMLPATPLHQFQSEYLSKRTDPVLPYVHLGKNEA 196 Query: 194 GISGIALFQDDRFVTKIPAKDGIIFALLRGNINQGELSVNLGERNGRKEMVMLSSMLNKR 253 + A+F +DRFV + + + + +LR + + + + E E + +S + + Sbjct: 197 IAAETAVFFNDRFVESLNSMETQLLVMLRNDGTSSQFTKEI-ESGSPFEQMTISFVDSHT 255 Query: 254 KVSVQHLGDNRFKFIIANSIHGSVLEYTGHEDLSQSKDRHKLEADIAEYISDKAQEVVRQ 313 V V+ G + F + + + EY E + K ++ + + Y+ QE+VR+ Sbjct: 256 TVDVEERGGEGWHFNVNLRLSAELTEYEKREHVLTDKKFAEIAEEFSRYLEKTCQEMVRR 315 Query: 314 LQ-QNNVDSIGIGQYVRNSLSYKAWKSL-NWRKVYPGVEVECRVKVTIKDYGKY 365 +Q Q+ D +G+G+Y R L SL WRK + + V + I G + Sbjct: 316 IQKQHQADPMGLGEYTRPLLPVDT--SLEEWRKKFTASTMSITVDLDIIHDGDF 367 >C0ZJ25.1 C0ZJ25_BREBN Spore germination protein Length = 367 Score = 142 bits (357), Expect = 8e-32, Method: Compositional matrix adjust. Identities = 90/347 (25%), Positives = 178/347 (51%), Gaps = 9/347 (2%) Query: 17 GCNSDQRILEKLGMMQTASYDLAENDQLKATLSVPVIDPESSEHRHLLTAVTDSLKEARV 76 GC +Q +LEKLG+ YDL+++ +L T IDPE+ E +++ + K V Sbjct: 19 GC-GEQHVLEKLGLAVAVGYDLSKDGKLLGTTVFYQIDPEARELVTVISGTAHTSKGLTV 77 Query: 77 QLSNQIDLLLVSGQLRSVLFGLSLAKEGLADHVDTLIRDPSIALGVQVTVVDGDAGKLLS 136 + + +V GQ+R V++ LA++G+ V+ L RD SI V +TV G A +L+ Sbjct: 78 SQNRESSKKIVGGQIRVVVYHDELARKGILSLVENLTRDASIGSNVYLTVAKGRAYDILT 137 Query: 137 KTFKPNKDTGTYVNHLLKKESSSNIIPPTTLYEFTREYYDDGIDPIAPIVKDGGDRAGIS 196 + N + G Y+ +++ + + L+EF ++YY G DP+ P ++ + I+ Sbjct: 138 HRYPENSNIGIYLYQAIRQNVEGENMISSKLHEFMKDYYAIGKDPVMPYIERVENELHIN 197 Query: 197 GIALFQDDRFVTKIPAKDGIIFALLRGNINQGELSVNLGERNGRKEMVMLSSMLNKRKVS 256 I LF+D + V +I +++G +LR N+GEL +N+ + ++ ++ + +K+++ Sbjct: 198 QIGLFRDQKLVAEITSREGFFLKILRERYNKGELEINIS-LDKKRFFTVIEHISSKKQIK 256 Query: 257 VQHLGDNRFKFIIANSIHGSVLEYTGHEDLSQSKDRHKLEADIAEYISDKAQEVVRQLQQ 316 V L RF + ++ + E DLS+ + K++ I + + + ++ + ++ Sbjct: 257 VLDLEKPRFS--VKVNMTAQIQEMAEKIDLSKPEQLKKVQKAIELAVKKEMEHLLEKFRK 314 Query: 317 NNVDSIGIGQYVRNSLSYKAWKSL----NWRKVYPGVEVECRVKVTI 359 +VD +G G+ + N S ++ K WR++ + + VK+ I Sbjct: 315 QDVDPVGFGE-IYNIASQRSGKKKMSKDEWREIMKRAKFDVDVKIKI 360 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.316 0.134 0.376 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,036,810,568 Number of extensions: 83556925 Number of successful extensions: 227875 Number of sequences better than 1.0e-05: 527 Number of HSP's gapped: 227120 Number of HSP's successfully gapped: 527 Length of query: 366 Length of database: 3,060,696,203 Length adjustment: 137 Effective length of query: 229 Effective length of database: 1,770,017,148 Effective search space: 405333926892 Effective search space used: 405333926892 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 132 (55.5 bits)