BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0336.0002.p (329 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KAC4.1 B3KAC4_9BACI Phosphate transporter 509 e-142 B1D8Z5.1 B1D8Z5_9BACL Phosphate transporter 437 e-121 C1T9F3.1 C1T9F3_9FIRM Phosphate/sulfate permease 395 e-108 A4J7W1.1 A4J7W1_DESRM Phosphate transporter 384 e-104 A8FC78.1 A8FC78_BACP2 PiT family inorganic phosphate transporter 382 e-104 >B3KAC4.1 B3KAC4_9BACI Phosphate transporter Length = 329 Score = 509 bits (1311), Expect = e-142, Method: Compositional matrix adjust. Identities = 249/329 (75%), Positives = 290/329 (88%) Query: 1 METSLFVLGFVIFLALAFDFINGFHDTANAIATSVSTRALRPRTAIIMAASMNFIGALIF 60 M+T++ VL V+FLALAFDFINGFHDTANAIATSVSTRAL+PR AI++AASMNF+GA++F Sbjct: 1 MDTTMLVLVIVVFLALAFDFINGFHDTANAIATSVSTRALKPRVAILLAASMNFVGAMMF 60 Query: 61 TGVAKKIGGSITDPSLLDNGVDIVIATLIAAIIWNLVTWWLGIPSSSSHXXXXXXXXXXX 120 TGVAK IGGS+ DP+ LDNG+++VIATL+AAIIWNLVTWW G+PSSSSH Sbjct: 61 TGVAKTIGGSVADPTKLDNGIEVVIATLLAAIIWNLVTWWFGLPSSSSHALIGALAGAVF 120 Query: 121 XXXXTEHIKWHGFIEIVEGLVFSPLIAFVVGYIVMTILKWIFANRSPHTVNKGFRSMQVI 180 ++ + + GF +IV LV SP+IAFV+GY+VM +LK IFA RSPHTVNKGFR+MQ+ Sbjct: 121 VGAGSDMLNYGGFKDIVLALVLSPIIAFVIGYLVMQLLKVIFAKRSPHTVNKGFRTMQIF 180 Query: 181 TAALQSFTHGTNDAQKAMGIITFALVTSGRLDTMEVPLWVKIAAATSMALGTSIGGWKII 240 TAALQSFTHGTNDAQKAMGIITFALVTSG+L MEVP WVK++AAT+MALGTS+GGWKII Sbjct: 181 TAALQSFTHGTNDAQKAMGIITFALVTSGQLQEMEVPFWVKVSAATAMALGTSVGGWKII 240 Query: 241 KTMGTKIFKIEPINGFAADISAASVIFSATLLHLPVSTTHAITSSILGVGSAKRFSAVKW 300 KTMGTKIFKIEPINGFAAD SAASVIFSATLLHLP+STTHAITS+ILGVGSAKRFSAVKW Sbjct: 241 KTMGTKIFKIEPINGFAADFSAASVIFSATLLHLPISTTHAITSAILGVGSAKRFSAVKW 300 Query: 301 GVAGRIVVTWFITIPISAVLAGLVFKLIF 329 +AGRIVVTWFITIPI+AVLAG++FK++F Sbjct: 301 SLAGRIVVTWFITIPITAVLAGVIFKILF 329 >B1D8Z5.1 B1D8Z5_9BACL Phosphate transporter Length = 331 Score = 437 bits (1124), Expect = e-121, Method: Compositional matrix adjust. Identities = 223/318 (70%), Positives = 257/318 (80%) Query: 11 VIFLALAFDFINGFHDTANAIATSVSTRALRPRTAIIMAASMNFIGALIFTGVAKKIGGS 70 V+ LAL FDFINGFHDTANAIAT+VSTRAL+PR AIIMAA MNFIGA+ FTGVAKKIG Sbjct: 9 VVILALGFDFINGFHDTANAIATTVSTRALKPRVAIIMAACMNFIGAVTFTGVAKKIGSG 68 Query: 71 ITDPSLLDNGVDIVIATLIAAIIWNLVTWWLGIPSSSSHXXXXXXXXXXXXXXXTEHIKW 130 + +P+ L NGV+IVIA LI+AI WNL+TWW GIPSSSSH + I Sbjct: 69 VANPADLSNGVEIVIAALISAIAWNLLTWWFGIPSSSSHALIGSLAGAVASGAGFDAINA 128 Query: 131 HGFIEIVEGLVFSPLIAFVVGYIVMTILKWIFANRSPHTVNKGFRSMQVITAALQSFTHG 190 HGFI+IV+ L+FSPLIAF G+I+M ILK IFA SPH VNKGFR Q++TAA QSF+HG Sbjct: 129 HGFIDIVKALIFSPLIAFSAGFIIMWILKLIFARTSPHQVNKGFRFGQILTAAFQSFSHG 188 Query: 191 TNDAQKAMGIITFALVTSGRLDTMEVPLWVKIAAATSMALGTSIGGWKIIKTMGTKIFKI 250 TNDAQKAMGIITFALV G D EVPLWVK++AA +MALGTSIGGWKIIKTMGTKIFKI Sbjct: 189 TNDAQKAMGIITFALVAGGIQDHFEVPLWVKMSAALAMALGTSIGGWKIIKTMGTKIFKI 248 Query: 251 EPINGFAADISAASVIFSATLLHLPVSTTHAITSSILGVGSAKRFSAVKWGVAGRIVVTW 310 EPINGFAAD+ +A VI SAT+L LPVSTTH ITSSILGVGSAKRFS+V+W +AG+IV+ W Sbjct: 249 EPINGFAADVGSAGVILSATMLELPVSTTHVITSSILGVGSAKRFSSVRWSLAGKIVIAW 308 Query: 311 FITIPISAVLAGLVFKLI 328 FITIPI+A+ A V+ +I Sbjct: 309 FITIPITALFAAGVYWVI 326 >C1T9F3.1 C1T9F3_9FIRM Phosphate/sulfate permease Length = 332 Score = 395 bits (1016), Expect = e-108, Method: Compositional matrix adjust. Identities = 184/325 (56%), Positives = 254/325 (78%) Query: 1 METSLFVLGFVIFLALAFDFINGFHDTANAIATSVSTRALRPRTAIIMAASMNFIGALIF 60 M+++L ++ V+ LAL+FDFINGFHDTANAIATS+ST+AL PR AI++A++MN +GAL F Sbjct: 1 MDSTLVLIAAVVLLALSFDFINGFHDTANAIATSISTKALTPRAAIVLASTMNLVGALTF 60 Query: 61 TGVAKKIGGSITDPSLLDNGVDIVIATLIAAIIWNLVTWWLGIPSSSSHXXXXXXXXXXX 120 TGVAK IGG I DP L++G+ IVIA L+AAIIWNL+TW+ GIPSSSSH Sbjct: 61 TGVAKTIGGKIADPMKLEHGLPIVIAALVAAIIWNLITWYYGIPSSSSHALIGALTGSVI 120 Query: 121 XXXXTEHIKWHGFIEIVEGLVFSPLIAFVVGYIVMTILKWIFANRSPHTVNKGFRSMQVI 180 + + GF+ I++ L+ SP++AF +GYI+M+IL+++F + +P + N+ FR +Q++ Sbjct: 121 ASAGLSAVNFAGFLTILQALILSPVLAFCIGYIIMSILRFVFRSANPRSTNRMFRFLQIL 180 Query: 181 TAALQSFTHGTNDAQKAMGIITFALVTSGRLDTMEVPLWVKIAAATSMALGTSIGGWKII 240 TAA Q+F+HGTNDAQK+MGIITFAL+ G +T ++P WVK+ A +MALGTS+GGWKII Sbjct: 181 TAAFQAFSHGTNDAQKSMGIITFALIAGGLQNTHDIPFWVKLTCAIAMALGTSVGGWKII 240 Query: 241 KTMGTKIFKIEPINGFAADISAASVIFSATLLHLPVSTTHAITSSILGVGSAKRFSAVKW 300 KT+GT+I K++PI+GFA+D+++ASVI AT++ PVSTTH I+S+I+GVGSAKR S VKW Sbjct: 241 KTVGTQIIKLKPISGFASDVTSASVIIGATIIGQPVSTTHVISSAIMGVGSAKRLSCVKW 300 Query: 301 GVAGRIVVTWFITIPISAVLAGLVF 325 A R++ W IT+PI+A LAGLV+ Sbjct: 301 CTAQRMITAWLITLPITATLAGLVY 325 >A4J7W1.1 A4J7W1_DESRM Phosphate transporter Length = 332 Score = 384 bits (985), Expect = e-104, Method: Compositional matrix adjust. Identities = 192/328 (58%), Positives = 255/328 (77%), Gaps = 1/328 (0%) Query: 1 METSLFVLGFVIFLALAFDFINGFHDTANAIATSVSTRALRPRTAIIMAASMNFIGALIF 60 ME + ++ V+ LAL+FDFINGFHDTANAIATS+ST+AL+PRTAI++AA MN +GAL F Sbjct: 1 MEQQIVIIAVVV-LALSFDFINGFHDTANAIATSISTKALKPRTAIVLAACMNLLGALTF 59 Query: 61 TGVAKKIGGSITDPSLLDNGVDIVIATLIAAIIWNLVTWWLGIPSSSSHXXXXXXXXXXX 120 TGVAK +GG + +P+ +++GV +V A LIAAIIWNL+TW+ GIPSSSSH Sbjct: 60 TGVAKTVGGKVANPANIEHGVYVVAAALIAAIIWNLITWYYGIPSSSSHALIGSLAGSVI 119 Query: 121 XXXXTEHIKWHGFIEIVEGLVFSPLIAFVVGYIVMTILKWIFANRSPHTVNKGFRSMQVI 180 + + GF+ I+EGL+ SPL+AF VGYI+M+I++ IFAN SPH +N FR +QV Sbjct: 120 AAAGFTAVNFKGFLTIIEGLILSPLLAFTVGYIIMSIIRMIFANVSPHKLNSNFRFLQVF 179 Query: 181 TAALQSFTHGTNDAQKAMGIITFALVTSGRLDTMEVPLWVKIAAATSMALGTSIGGWKII 240 TAA Q+F+HGTNDAQK MGIITFALV G ++++V LWVK++AA +MALGTS+GGW+II Sbjct: 180 TAAFQAFSHGTNDAQKTMGIITFALVAGGFQESLDVALWVKVSAAMAMALGTSVGGWRII 239 Query: 241 KTMGTKIFKIEPINGFAADISAASVIFSATLLHLPVSTTHAITSSILGVGSAKRFSAVKW 300 KT+G+KI K EP +GFA+D+++A VI ATL+ LPVSTTH I+S+I+GVGSAKRFS+V+W Sbjct: 240 KTVGSKIIKFEPASGFASDLTSALVITGATLIKLPVSTTHVISSAIMGVGSAKRFSSVQW 299 Query: 301 GVAGRIVVTWFITIPISAVLAGLVFKLI 328 G A +V W IT+PI+ +LA L F ++ Sbjct: 300 GTAVTMVSAWIITLPITMILASLTFIIV 327 >A8FC78.1 A8FC78_BACP2 PiT family inorganic phosphate transporter Length = 332 Score = 382 bits (982), Expect = e-104, Method: Compositional matrix adjust. Identities = 190/325 (58%), Positives = 239/325 (73%) Query: 1 METSLFVLGFVIFLALAFDFINGFHDTANAIATSVSTRALRPRTAIIMAASMNFIGALIF 60 M+T L + F++ ALAFDFINGFHDTANAIATSVST+AL+PR AIIMAA MNF+GA+ F Sbjct: 1 MDTLLILTIFIVICALAFDFINGFHDTANAIATSVSTKALKPRHAIIMAAFMNFLGAMTF 60 Query: 61 TGVAKKIGGSITDPSLLDNGVDIVIATLIAAIIWNLVTWWLGIPSSSSHXXXXXXXXXXX 120 TGVAK I I DP L NG +++A LIAAI WNL+TW+ GIPSSSSH Sbjct: 61 TGVAKSITKDIADPFTLQNGSVVILAALIAAITWNLLTWYYGIPSSSSHALIGSIAGAVI 120 Query: 121 XXXXTEHIKWHGFIEIVEGLVFSPLIAFVVGYIVMTILKWIFANRSPHTVNKGFRSMQVI 180 + + GFI+I++ L+ SP++AFV+GYIV TI+K+IF N + NK FR +Q++ Sbjct: 121 ASAGFGALNYSGFIKIIQALLLSPILAFVLGYIVYTIVKFIFRNNNLSKTNKQFRRVQIL 180 Query: 181 TAALQSFTHGTNDAQKAMGIITFALVTSGRLDTMEVPLWVKIAAATSMALGTSIGGWKII 240 TAALQS+THGTNDAQKAMGIIT AL+ T ++P WV+ + A +M LGTSIGGWKII Sbjct: 181 TAALQSYTHGTNDAQKAMGIITMALIAGNLHTTDDIPFWVQFSCALAMGLGTSIGGWKII 240 Query: 241 KTMGTKIFKIEPINGFAADISAASVIFSATLLHLPVSTTHAITSSILGVGSAKRFSAVKW 300 KT+G KI KI P+NG +AD++ A++IF AT +HLPVSTTH I+SSILGVGSA R V W Sbjct: 241 KTVGGKIMKIRPVNGVSADLTGAAIIFGATFIHLPVSTTHVISSSILGVGSAHRVKGVNW 300 Query: 301 GVAGRIVVTWFITIPISAVLAGLVF 325 G A R++VTW IT+PIS L L++ Sbjct: 301 GTAKRMLVTWVITLPISGTLGALIY 325 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.326 0.137 0.413 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,704,906,688 Number of extensions: 62469008 Number of successful extensions: 248438 Number of sequences better than 1.0e-05: 1554 Number of HSP's gapped: 244227 Number of HSP's successfully gapped: 2214 Length of query: 329 Length of database: 3,060,696,203 Length adjustment: 136 Effective length of query: 193 Effective length of database: 1,779,438,163 Effective search space: 343431565459 Effective search space used: 343431565459 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 131 (55.1 bits)