BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0335.0021.p (290 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value A8MFN7.1 A8MFN7_ALKOO Methyltransferase type 11 274 8e-72 A6FF73.1 A6FF73_9GAMM SAM-dependent methyltransferase 188 5e-46 Q1Z3F0.1 Q1Z3F0_PHOPR SAM-dependent methyltransferase 186 2e-45 B7VNY9.1 B7VNY9_VIBSL Putative SAM dependant methyltransferase 184 6e-45 A3XWK3.1 A3XWK3_9VIBR SAM-dependent methyltransferase 181 6e-44 >A8MFN7.1 A8MFN7_ALKOO Methyltransferase type 11 Length = 266 Score = 274 bits (700), Expect = 8e-72, Method: Compositional matrix adjust. Identities = 120/265 (45%), Positives = 188/265 (70%), Gaps = 1/265 (0%) Query: 26 MKGIINYYDRYNEAARLTTDHARRLEFITTTHFLDTYLKGDSQILDLGAGAGIYTFYYAQ 85 MK ++++Y++Y+E +R+TT++AR++EF+ TT LD +++ +IL++GAG G Y+FYY + Sbjct: 1 MKTVVDFYEKYDEESRITTNNARKIEFLMTTGVLDNHIEKTHRILEIGAGTGAYSFYYGE 60 Query: 86 KGHTVISTDLTPKHVEIISQRASDEGY-ANITAAMADATDLSRYPDESFDAVLCLGPLYH 144 KG+ VI+TD+TPKH+EII Q+ + G N+ A +A+ATDLS++ ESFD VLCLGP+YH Sbjct: 61 KGNFVIATDITPKHIEIIGQKMKERGNDINLQAEVANATDLSQFSSESFDVVLCLGPMYH 120 Query: 145 LTDIPDQDTCIRECLRVLRPGGLLAAAYVNRLFIYPHLVKSDPGFLDTRWIQTILEEKQI 204 LT+ D+ TC+ E LRVL+ GGLLA AY+N+ F+ ++ + FL +I IL+ Sbjct: 121 LTNSSDRKTCLSEALRVLKKGGLLAIAYINKHFVLNSVMIRNKEFLTRNFIDKILDTGVT 180 Query: 205 SSTEADCFWTDAFFHTPGEIEALIEAYGTQKLVHAASDGISILLRETLNGLTTEQFNIWV 264 E +CFWTDAFF TP E+ + + + + + H DG+S ++ ++G+ +E++ W+ Sbjct: 181 REGEKECFWTDAFFTTPDEMASYMNCFEVEIIDHLGVDGLSPYFKDAVDGMNSEEYESWL 240 Query: 265 DYHLRTCTEPSILGMSNHGLYLCRK 289 Y++R+C E SILG+SNHGL LC+K Sbjct: 241 YYNMRSCREKSILGISNHGLLLCKK 265 >A6FF73.1 A6FF73_9GAMM SAM-dependent methyltransferase Length = 265 Score = 188 bits (478), Expect = 5e-46, Method: Compositional matrix adjust. Identities = 94/264 (35%), Positives = 148/264 (56%), Gaps = 1/264 (0%) Query: 26 MKGIINYYDRYNEAARLTTDHARRLEFITTTHFLDTYLKGDSQILDLGAGAGIYTFYYAQ 85 M+ +I+ Y NE +RLT + ++EF TT L YL + ++++LGA G Y+ ++A+ Sbjct: 1 MQELIDQYTGANEDSRLTRQYIAQMEFDTTMDALKAYLVPNVKVIELGAATGRYSLHFAK 60 Query: 86 KGHTVISTDLTPKHVEIISQRASDEGYANITAAMADATDLSRYPDESFDAVLCLGPLYHL 145 + +L P V I+ ++A ++G ++ +A + S D + LGPLYHL Sbjct: 61 MACDTTAIELVPDQVNILKRKAEEQGL-TLSIHEGNACSVPFIESNSHDLCVILGPLYHL 119 Query: 146 TDIPDQDTCIRECLRVLRPGGLLAAAYVNRLFIYPHLVKSDPGFLDTRWIQTILEEKQIS 205 +D I E R+L+P G+LA AY++R FI + P + + T+LE + Sbjct: 120 QTQEQRDQAISEAYRILKPNGVLAVAYISRYFIAGMFAQQFPKLITPEILSTLLESGLVP 179 Query: 206 STEADCFWTDAFFHTPGEIEALIEAYGTQKLVHAASDGISILLRETLNGLTTEQFNIWVD 265 S AD F+ +F TP E+E L+ A+G +L H A+DG + + +N T EQ+ W+ Sbjct: 180 SQLADSFFNVGYFATPVEVEELVSAHGFTQLRHVATDGFNRYISSGVNNFTPEQYQTWLS 239 Query: 266 YHLRTCTEPSILGMSNHGLYLCRK 289 YHL+TC EPS+LG SNHGL + RK Sbjct: 240 YHLKTCDEPSLLGSSNHGLVIARK 263 >Q1Z3F0.1 Q1Z3F0_PHOPR SAM-dependent methyltransferase Length = 265 Score = 186 bits (472), Expect = 2e-45, Method: Compositional matrix adjust. Identities = 92/264 (34%), Positives = 147/264 (55%), Gaps = 1/264 (0%) Query: 26 MKGIINYYDRYNEAARLTTDHARRLEFITTTHFLDTYLKGDSQILDLGAGAGIYTFYYAQ 85 M+ +I+ Y +E RLT ++EF TT H L YL + ++++ GA G Y+ +A+ Sbjct: 1 MQELIDQYTGVDEDGRLTRQFITQMEFDTTMHVLKEYLTPEIKVIEQGAATGRYSLNFAK 60 Query: 86 KGHTVISTDLTPKHVEIISQRASDEGYANITAAMADATDLSRYPDESFDAVLCLGPLYHL 145 G + +L P V I+ ++A+++G ++ +A + +S D + LGPLYHL Sbjct: 61 MGCETTAIELVPDQVNILKRKATEQGL-TLSIYEGNACSVPFVESDSHDLCVILGPLYHL 119 Query: 146 TDIPDQDTCIRECLRVLRPGGLLAAAYVNRLFIYPHLVKSDPGFLDTRWIQTILEEKQIS 205 ++D I E R+L+PGG+LA AY++R F+ + P + + T+ E + Sbjct: 120 QTQKERDQAISEAHRILKPGGVLAVAYISRYFVAGMFAQRFPELVTPEVLSTLFESGLVP 179 Query: 206 STEADCFWTDAFFHTPGEIEALIEAYGTQKLVHAASDGISILLRETLNGLTTEQFNIWVD 265 + AD F+ +F +P EIE L+ +G KL H A+DG + +N T EQ+ W+ Sbjct: 180 NKLADSFFNVGYFASPSEIERLVSEHGFTKLRHVATDGFGRYISSGVNKFTPEQYKTWLS 239 Query: 266 YHLRTCTEPSILGMSNHGLYLCRK 289 YHL+TC EPS+LG SNHGL + RK Sbjct: 240 YHLKTCDEPSLLGSSNHGLVISRK 263 >B7VNY9.1 B7VNY9_VIBSL Putative SAM dependant methyltransferase Length = 269 Score = 184 bits (468), Expect = 6e-45, Method: Compositional matrix adjust. Identities = 94/264 (35%), Positives = 144/264 (54%), Gaps = 1/264 (0%) Query: 26 MKGIINYYDRYNEAARLTTDHARRLEFITTTHFLDTYLKGDSQILDLGAGAGIYTFYYAQ 85 M+ +I Y NE RLT + +LEF TT H L YL + +LGA G Y+ ++A+ Sbjct: 1 MEELIAQYTGANEDERLTRQYIAQLEFDTTIHKLTPYLLKGKSVCELGAATGRYSLHFAK 60 Query: 86 KGHTVISTDLTPKHVEIISQRASDEGYANITAAMADATDLSRYPDESFDAVLCLGPLYHL 145 G V + +L P V+I++ +A EG + +A D+S S D + LGPLYHL Sbjct: 61 LGCDVTAVELAPDQVKILNTKAKSEGI-ELKVFEGNACDVSFIESSSLDVCVILGPLYHL 119 Query: 146 TDIPDQDTCIRECLRVLRPGGLLAAAYVNRLFIYPHLVKSDPGFLDTRWIQTILEEKQIS 205 + +++ + E LR+L+P G+LA AY++R F+ + P + + + + +S Sbjct: 120 KTLHERELAVNEALRILKPNGVLAIAYISRFFVAGMFAQQFPELVTPEVLLELNKHGTVS 179 Query: 206 STEADCFWTDAFFHTPGEIEALIEAYGTQKLVHAASDGISILLRETLNGLTTEQFNIWVD 265 + +AD F+ +F TP EIE L+ G + HAA+DG + +N T Q+ W+D Sbjct: 180 NAKADSFFKVGYFATPSEIENLVNKNGFNVIGHAATDGFGRYISNGVNNFTPSQYQTWLD 239 Query: 266 YHLRTCTEPSILGMSNHGLYLCRK 289 YHL C E S+LG SNHGL + RK Sbjct: 240 YHLANCDEASLLGSSNHGLVIARK 263 >A3XWK3.1 A3XWK3_9VIBR SAM-dependent methyltransferase Length = 269 Score = 181 bits (460), Expect = 6e-44, Method: Compositional matrix adjust. Identities = 93/264 (35%), Positives = 143/264 (54%), Gaps = 1/264 (0%) Query: 26 MKGIINYYDRYNEAARLTTDHARRLEFITTTHFLDTYLKGDSQILDLGAGAGIYTFYYAQ 85 M+ +I Y NE RLT + +LEF TT H L YL + +LGA G Y+ ++A+ Sbjct: 1 MEELIAQYTGANEDERLTRQYIAQLEFDTTIHKLTPYLLKGKSVCELGAATGRYSLHFAK 60 Query: 86 KGHTVISTDLTPKHVEIISQRASDEGYANITAAMADATDLSRYPDESFDAVLCLGPLYHL 145 G V + +L P V+I++ + EG + +A D+S S D + LGPLYHL Sbjct: 61 LGCDVTAVELAPDQVKILNTKVKSEGL-ELKVFEGNACDVSFIESSSQDVCVILGPLYHL 119 Query: 146 TDIPDQDTCIRECLRVLRPGGLLAAAYVNRLFIYPHLVKSDPGFLDTRWIQTILEEKQIS 205 + +++ + E LR+L+P G+LA AY++R F+ + P + + + + +S Sbjct: 120 KTLHERELAVNEALRILKPNGVLAIAYISRFFVAGMFAQQFPELVTPEVLLELNKHGTVS 179 Query: 206 STEADCFWTDAFFHTPGEIEALIEAYGTQKLVHAASDGISILLRETLNGLTTEQFNIWVD 265 + +AD F+ +F TP EIE L+ G + HAA+DG + +N T Q+ W+D Sbjct: 180 NAKADSFFKVGYFATPSEIENLVNKNGFNVIGHAATDGFGRYISNGVNNFTPSQYQTWLD 239 Query: 266 YHLRTCTEPSILGMSNHGLYLCRK 289 YHL C E S+LG SNHGL + RK Sbjct: 240 YHLANCDEASLLGSSNHGLVIARK 263 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.321 0.137 0.416 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,775,666,431 Number of extensions: 71098482 Number of successful extensions: 193808 Number of sequences better than 1.0e-05: 526 Number of HSP's gapped: 195891 Number of HSP's successfully gapped: 526 Length of query: 290 Length of database: 3,060,696,203 Length adjustment: 134 Effective length of query: 156 Effective length of database: 1,798,280,193 Effective search space: 280531710108 Effective search space used: 280531710108 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 130 (54.7 bits)