BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0335.0018.p (534 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K9R1.1 B3K9R1_9BACI Chemotaxis sensory transducer 131 2e-28 Q97MS1.1 Q97MS1_CLOAB Membrane-associated methyl-accepting chemo... 116 6e-24 B8I4Z5.1 B8I4Z5_CLOCE Methyl-accepting chemotaxis sensory transd... 105 1e-20 B3K102.1 B3K102_9DELT Methyl-accepting chemotaxis sensory transd... 104 3e-20 A5G585.1 A5G585_GEOUR Methyl-accepting chemotaxis sensory transd... 92 1e-16 >B3K9R1.1 B3K9R1_9BACI Chemotaxis sensory transducer Length = 662 Score = 131 bits (330), Expect = 2e-28, Method: Compositional matrix adjust. Identities = 86/261 (32%), Positives = 125/261 (47%), Gaps = 3/261 (1%) Query: 215 IIARSISKPLMRMLGLATEVASGNLTQR--AD-ISTKDEVGQLASALNRMVDNLKDLING 271 ++ RSI KPL + + ++ G+ TQ AD + DE+G L+ +++++++++I Sbjct: 340 LLTRSIVKPLGVSVAIGESMSKGDFTQEVPADYLRRADEIGTLSRVFAQLLESMREMIGK 399 Query: 272 IVMNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXNITELFKEXXXXXXXXXXXXXXXXXX 331 + MN ++ELF+E Sbjct: 400 VNMNASSVAAASQQLSAGAEELAVGSSSQAESVQTVSELFQEWSNAINSVASDASAASEL 459 Query: 332 XXXTVRTAREGGNVVETSLQGMQAVNYQMTQLEEDSSKIGDIIEVIDDIAEQTXXXXXXX 391 T A EG +V+ S++GM + + +LE DS +IG+IIEVIDDIA+QT Sbjct: 460 VNQTSGIAAEGSGIVQQSVEGMNRIREHVAKLETDSQRIGEIIEVIDDIAKQTNLLALNA 519 Query: 392 XXXXXXXXXXXXXXXVVADEVRKLAERSSDATKEITKIIKAMQENTKQSVRAVAESVEQS 451 VVADEVRKLAERS ATK+I IIK MQ+NT SV AV + V + Sbjct: 520 TIEAARAGDQGRGFAVVADEVRKLAERSGGATKQIADIIKGMQKNTADSVEAVHQGVIHT 579 Query: 452 SMTSQAFEQIISMVNSSSLKV 472 +AF I SMVN S+ KV Sbjct: 580 EKAGEAFAHITSMVNESAGKV 600 >Q97MS1.1 Q97MS1_CLOAB Membrane-associated methyl-accepting chemotaxis protein with HAMP domain Length = 555 Score = 116 bits (290), Expect = 6e-24, Method: Compositional matrix adjust. Identities = 106/469 (22%), Positives = 195/469 (41%), Gaps = 19/469 (4%) Query: 1 MKWFGNLKTATKIISAFLIVSLILAGLGVFSISTLRNANGNMKAMYGNNLISVRDLSAAQ 60 M+W+ ++K ++KI+ F+++ I A GVF I L N + Y L+ +L + Sbjct: 1 MRWYDDMKISSKILVGFVVIIFISAVSGVFGILNLNKVNNSNTETYEKMLVPTGELVSIS 60 Query: 61 INYQTMRVYLRDMSTTTVSGEVSGYKEKIETTRQEINKLVDRYRPLATTLKEQELLRGFD 120 ++ TMR +RD+ T ++ + + +K+++ + T + +L Sbjct: 61 NDFGTMRARVRDVVIETQMDKMDKFSSDFNAASSDFDKVLESFSQTILTADGKTILSEIK 120 Query: 121 TEYGEYQKLFDQALILAEQDDPTEFNAFLRSQLTPQGEKVVVNLKGLIDVNVDLAXXXXA 180 EY + QA+ L++Q+ E A +R+QLT +K+ ++K + D+ +LA Sbjct: 121 DAKKEYVQYAQQAMELSKQNKNVEAMAIVRNQLTVAHDKLATSIKNVTDMKKELAK---- 176 Query: 181 DEATSHSQSTYSSALILTXXXXXXXXXXXXXXXYIIARSISKPLMRMLGLATEVASGNLT 240 E+T + +T +++I T + +RSI P+ ++ ++ ++A G L Sbjct: 177 -ESTDQNSATAKTSIIETIILVILGAGLGVAMAVLTSRSIGNPMKKITEVSEKIADGELD 235 Query: 241 QRADISTKDEVGQLASALNRMVDNLKDLINGIVMNXXXXXXXXXXXXXXXXXXXXXXXXX 300 KDE+G L + N+MV NL +++ + Sbjct: 236 VELKTDRKDEIGVLMNTFNKMVSNLNLVMSSMNTAADQVAIGSKQVSDSSIMLSQGSSDQ 295 Query: 301 XXXXXNITELFKEXXXXXXXXXXXXXXXXXXXXXTVRTAREGGNVVETSLQGMQAVNYQM 360 +T +E A +G N ++ L M +N Sbjct: 296 ASAVEELTSSMEEIASQTKVNANNATEASELGNEVKSNAEKGNNQMKQMLNSMDDINAA- 354 Query: 361 TQLEEDSSKIGDIIEVIDDIAEQTXXXXXXXXXXXXXXXXXXXXXXVVADEVRKLAERSS 420 S I II+VIDDIA QT VVA+EVR LA +S+ Sbjct: 355 ------SKNISKIIKVIDDIAFQTNILSLNAAVEAARAGQYGKGFAVVAEEVRNLAAKSA 408 Query: 421 DATKEITKIIKAMQENTKQSVRAVAESVEQSSMTSQAFEQIISMVNSSS 469 +A +E T++I + S+R V E + T+ A ++I+ ++ S+ Sbjct: 409 NAARETTEMI-------ENSIRKVETGTEFAKETAAALDEIVQGISKSA 450 >B8I4Z5.1 B8I4Z5_CLOCE Methyl-accepting chemotaxis sensory transducer Length = 567 Score = 105 bits (261), Expect = 1e-20, Method: Compositional matrix adjust. Identities = 105/435 (24%), Positives = 178/435 (40%), Gaps = 18/435 (4%) Query: 1 MKWFGNLKTATKIISAFLIVSLILAGLGVFSISTLRNANGNMKAMYGNNLISVRDLSAAQ 60 MKWF NLK TK+++ F+IV+L+ +G+ I ++ N +Y N I+ ++ Sbjct: 1 MKWFNNLKIGTKLLATFIIVALLAGVVGIVGIINIKKEEKNYTELYKNFGIATANIGKIS 60 Query: 61 INYQTMRVYLRDMSTTTVSGEVSGYKEKIETTRQEIN-KLVDRYRPLATTLKEQELLRGF 119 ++ ++R RD+ + + Y +I+ +++ L + + L T KEQ+ Sbjct: 61 TDFNSIRAMTRDILLSNSVEDKKNYSNQIKDLDKDVQLNLKELFDSLQTE-KEQKAYTAL 119 Query: 120 DTEYGEYQKLFDQA--LILAEQDDPTEFNAFLRSQLTPQGEKVVVNLKGLIDVNVDLAXX 177 + + +Y ++ D+ + +A QDD F + P K+ K ID +L Sbjct: 120 NEDLNKYDEIRDRVINMAIAGQDDEA-LTLFYKEGTEPA--KLA---KQYIDELFELKET 173 Query: 178 XXADEATSHSQSTYSSALILTXXXXXXXXXXXXXXXYIIARSISKPLMRMLGLATEVASG 237 + +S+ T ++ + + I+R IS P+ R++ A ++A G Sbjct: 174 GGVERTEEYSRETDATVYTMLTVVVIAVIVAVLLGIF-ISRLISVPVNRLVIAAEKIADG 232 Query: 238 NLTQRADISTKDEVGQLASALNRMVDNLKDLINGIVMNXXXXXXXXXXXXXXXXXXXXXX 297 +L ++KDE+G LASA RM DNL D+I+ I Sbjct: 233 DLNVDVKQNSKDEIGMLASAFKRMSDNLNDVISSINSAAEQVSSGSRQVSDSSVALSQGA 292 Query: 298 XXXXXXXXNITELFKEXXXXXXXXXXXXXXXXXXXXXTVRTAREGGNVVETSLQGMQAVN 357 +T +E A +G ++ L M +N Sbjct: 293 TEQASSIEELTASLEEISAQTKLNAENATQASNLAENVKTIALKGNQHMKEMLNAMDDIN 352 Query: 358 YQMTQLEEDSSKIGDIIEVIDDIAEQTXXXXXXXXXXXXXXXXXXXXXXVVADEVRKLAE 417 + SS I II+VID+IA QT VVA+EVR LA Sbjct: 353 -------DSSSNISKIIKVIDEIAFQTNILALNAAVEAARAGQHGKGFAVVAEEVRNLAA 405 Query: 418 RSSDATKEITKIIKA 432 RS++A KE T +I+ Sbjct: 406 RSANAAKETTDMIEG 420 >B3K102.1 B3K102_9DELT Methyl-accepting chemotaxis sensory transducer Length = 563 Score = 104 bits (259), Expect = 3e-20, Method: Compositional matrix adjust. Identities = 89/291 (30%), Positives = 134/291 (46%), Gaps = 46/291 (15%) Query: 1 MKWFGNLKTATKIISAFLIVSLILAGLGVFSISTLRNANGNMKAMYGNNLISVRDLSAAQ 60 MKWF NLK TK+ISAF++VSLI A +G I + N MY L+ + + A Sbjct: 1 MKWFSNLKVGTKLISAFIVVSLITAIVGYIGIRNMGAINDMADEMYQKELLGISYIKEAN 60 Query: 61 INY----QTMRVYL-------RDMSTTTVSGEVSGYKEKIETTRQEINKLVDRYRPLATT 109 IN + + +L R+ S ++ +GYKE +D+ RP+ T+ Sbjct: 61 INLIYISRAEKNFLLATSAQERETSQGNINKYKAGYKE-----------WLDKARPMFTS 109 Query: 110 LKEQELLRGFDTEYGEY----QKLFD----QALILAEQDDPTEFNAFLRSQLTPQGEKVV 161 K +E+L+ + E+ QK+ D +AL +Q F Q+ Sbjct: 110 EKGKEILKRLEAANEEWFAVQQKVIDLGAKEALNDRKQSVELSFGEARTKQMA------- 162 Query: 162 VNLKGLIDVNV-DLA--XXXXADEATSHSQSTYSSALILTXXXXXXXXXXXXXXXYIIAR 218 +D N+ +LA A +A+ + Y S+L + IAR Sbjct: 163 ------VDDNLTELARLKEGNAKDASDETTRIYKSSLTMMVGLVAGGVLIGLALGIFIAR 216 Query: 219 SISKPLMRMLGLATEVASGNLTQRADISTKDEVGQLASALNRMVDNLKDLI 269 IS PL R + AT VA G+LT+ ++ KDEVGQLA+ALN MV LK+++ Sbjct: 217 MISVPLRRGVEFATSVAGGDLTRSINLDQKDEVGQLAAALNDMVGRLKEIV 267 >A5G585.1 A5G585_GEOUR Methyl-accepting chemotaxis sensory transducer Length = 566 Score = 92.4 bits (228), Expect = 1e-16, Method: Compositional matrix adjust. Identities = 83/279 (29%), Positives = 138/279 (49%), Gaps = 24/279 (8%) Query: 1 MKWFGNLKTATKIISAFLIVSLILAGLGVFSISTLRNANGNMKAMYGNNLISVRDLSAAQ 60 MKWF NLK K++ F +++LI G+GV I + + AMY N + L Sbjct: 1 MKWFYNLKIGKKLVIGFTLLALIAVGVGVLGILKINEIEESASAMYKMNTEPLMVLLDTA 60 Query: 61 INYQTMRVYLRDMSTTTVSGEVSGYKEKIETTRQEINKLVDRYRPLAT---TLKEQELLR 117 + +Q RV LRD+ E K K +E++K VD LAT T+K EL + Sbjct: 61 VPFQRTRVNLRDLILDKTIEE----KNKRVALIKELDKEVDD--GLATFEKTVKNAELKK 114 Query: 118 GFDTEYG---EYQKLFDQALILAEQDDPTEFNAFLRSQLTPQGEKVVVNLKGLID----V 170 +++ G +Y+ + ++ + LA D E ++RS P+ + L+ L+D + Sbjct: 115 EYESLKGLIAKYEPVREKIITLAMADKDEEAVTYMRS---PEAAGLAKELETLMDKLGDM 171 Query: 171 NVDLAXXXXADEATSHSQSTYSSALILTXXXXXXXXXXXXXXXYIIARSISKPLMRMLGL 230 V+LA + + +++ ++ L+LT IAR IS+PL + + Sbjct: 172 KVELARKKDIE---NEAEARKAATLMLTILCFGVAVAIGLG--LFIARIISRPLREAVEV 226 Query: 231 ATEVASGNLTQRADISTKDEVGQLASALNRMVDNLKDLI 269 A +A+G+LT + +TKDE GQL +A+ MV+ LKD++ Sbjct: 227 ANRLAAGDLTVVLESNTKDETGQLLAAMGNMVEKLKDVV 265 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.313 0.128 0.332 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,735,195,471 Number of extensions: 54962009 Number of successful extensions: 213413 Number of sequences better than 1.0e-05: 2999 Number of HSP's gapped: 218694 Number of HSP's successfully gapped: 3154 Length of query: 534 Length of database: 3,060,696,203 Length adjustment: 141 Effective length of query: 393 Effective length of database: 1,732,333,088 Effective search space: 680806903584 Effective search space used: 680806903584 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 134 (56.2 bits)