BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0335.0009.p (416 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value A8MEH6.1 A8MEH6_ALKOO Major facilitator superfamily MFS_1 350 1e-94 Q4V226.1 Q4V226_BACCZ Macrolide-efflux protein 342 5e-92 Q8TRL4.1 Q8TRL4_METAC Macrolide-efflux protein 331 7e-89 Q46AW2.1 Q46AW2_METBF Putative uncharacterized protein 327 2e-87 Q5WLF5.1 Q5WLF5_BACSK Major facilitator (MFS) superfamily macrol... 325 6e-87 >A8MEH6.1 A8MEH6_ALKOO Major facilitator superfamily MFS_1 Length = 421 Score = 350 bits (899), Expect = 1e-94, Method: Compositional matrix adjust. Identities = 181/372 (48%), Positives = 252/372 (67%), Gaps = 3/372 (0%) Query: 1 MGEWISSIGSGLTAFALGVYVFHMTGTATSVAMVTLCAYVPSIVLGPFGGVLADRFDRRL 60 +G+ ISSIGSG+TAFA+ +YV+ TG+AT V++ L A++P+I+L P GGVLADR+DRRL Sbjct: 9 IGQLISSIGSGMTAFAVSIYVYQSTGSATLVSLAALLAFLPTILLNPVGGVLADRYDRRL 68 Query: 61 MMITGDLGSAVGLVFILVLMLEGEPSLGQICLGVALSSVFVSLLEPAYKATITDLLSVEQ 120 MMI GD SA+GL+F+L+ + G L I +GV ++SVFVSLLEPAY+AT+TD++S E Sbjct: 69 MMILGDSLSALGLLFMLISIQTGHRGLMPIFIGVTINSVFVSLLEPAYRATVTDIISEEN 128 Query: 121 FAKASGLVQLAASSKYLLSPVLAGSLLAXXXXXXXXXXXXXXXXXXXXXXXXXKRSMNTP 180 +AKASGLVQ+A +SKYL+SP +AG +L K+ + Sbjct: 129 YAKASGLVQIAGTSKYLISPFIAGLILTVSDIRAILIFDIATIIITAFAVASVKKRIAVV 188 Query: 181 KSAAVTGVPLQLFKDLREGWKALASVQGVLQLIFVLSMVTFCAGFLQTLFTPLLLAFTDT 240 K FK+ G ++ S +GV L+ +++ + F F+QTL TP++L+F DT Sbjct: 189 KPITHN---FNFFKEFEAGVNSITSDRGVRSLVILMTFMCFFIAFIQTLMTPMILSFADT 245 Query: 241 RTLGMIESISAVGMLAGSLVIGIFSLKGNIVRLLAVSLGVAGLCFALVGMSTNTALITSA 300 + LG++ES+SAVGML GS++IGI ++K N ++L VSL AG+ ALVG +TN I + Sbjct: 246 KKLGIMESVSAVGMLTGSIIIGILNIKKNHPKILMVSLMAAGIFMALVGTTTNITFIVVS 305 Query: 301 GFLFFAALPFVNTSADVMIRSNIPGEVQGRAWGIIGVLSQFGYVAAYACSGVLSDQVFNP 360 FLFF ALPFVNT ADV+IR IP +VQGRAWG+I V++Q G+V AYA G L+D VF P Sbjct: 306 CFLFFTALPFVNTCADVLIRMRIPNDVQGRAWGMISVITQSGFVMAYAVCGALADYVFGP 365 Query: 361 LLQEGGMLASSV 372 +L EGG+ A ++ Sbjct: 366 MLMEGGIFAGNI 377 >Q4V226.1 Q4V226_BACCZ Macrolide-efflux protein Length = 441 Score = 342 bits (876), Expect = 5e-92, Method: Compositional matrix adjust. Identities = 170/371 (45%), Positives = 253/371 (68%), Gaps = 4/371 (1%) Query: 2 GEWISSIGSGLTAFALGVYVFHMTGTATSVAMVTLCAYVPSIVLGPFGGVLADRFDRRLM 61 G++IS+IG+GLT+F L +YVF TG A+ +A+VTL A++PS++L P GVLADR+DRRL+ Sbjct: 21 GQFISAIGNGLTSFGLSIYVFEQTGRASLMALVTLLAFMPSLLLSPVAGVLADRYDRRLL 80 Query: 62 MITGDLGSAVGLVFILVLMLEGEPSLGQICLGVALSSVFVSLLEPAYKATITDLLSVEQF 121 M+ GD S +GL+FIL+ ML GE + QIC+GV +SS+F S+L+PAYKAT+TDLL+ E++ Sbjct: 81 MVLGDSLSMLGLIFILICMLGGEAQIWQICVGVTISSIFSSMLDPAYKATVTDLLTEEEY 140 Query: 122 AKASGLVQLAASSKYLLSPVLAGSLLAXXXXXXXXXXXXXXXXXXXXXXXXXKRSMNTPK 181 KASG VQ+A S+KYL+SP++AG LL ++ + + K Sbjct: 141 TKASGFVQVAGSAKYLISPIIAGFLLTVVDIKLLLVIDICTFFVTVASTLAVRKGLISKK 200 Query: 182 SAAVTGVPLQLFKDLREGWKALASVQGVLQLIFVLSMVTFCAGFLQTLFTPLLLAFTDTR 241 +A ++L+EGW A++ +GVL L+ + S++TF GF++TL P++LAF D+ Sbjct: 201 NAQENSFT----RELKEGWDAVSKKRGVLVLVMMSSVLTFFLGFIETLSVPMILAFADSA 256 Query: 242 TLGMIESISAVGMLAGSLVIGIFSLKGNIVRLLAVSLGVAGLCFALVGMSTNTALITSAG 301 +G +E+ A GML S++IG +K +++L +SL G+ A+ G+ N L+ +G Sbjct: 257 VVGTVETTVATGMLVSSVLIGFIPIKKGFIKVLFISLFCEGIFMAVFGLRENIVLLCISG 316 Query: 302 FLFFAALPFVNTSADVMIRSNIPGEVQGRAWGIIGVLSQFGYVAAYACSGVLSDQVFNPL 361 FLFFA +PF NT+ D ++R+NI VQGRAW +IG++SQ G++AAY SGVL+D VF PL Sbjct: 317 FLFFAMMPFANTTLDFLVRTNIDNSVQGRAWSVIGLISQLGFIAAYTLSGVLADYVFTPL 376 Query: 362 LQEGGMLASSV 372 L +GG+LA S+ Sbjct: 377 LVDGGLLADSM 387 >Q8TRL4.1 Q8TRL4_METAC Macrolide-efflux protein Length = 443 Score = 331 bits (849), Expect = 7e-89, Method: Compositional matrix adjust. Identities = 166/367 (45%), Positives = 242/367 (65%), Gaps = 3/367 (0%) Query: 2 GEWISSIGSGLTAFALGVYVFHMTGTATSVAMVTLCAYVPSIVLGPFGGVLADRFDRRLM 61 G++IS IGSGLT F+LG+YV+ TGTA+S + +CA++P +L P+GG+LADR DRRLM Sbjct: 18 GQFISIIGSGLTTFSLGIYVYQQTGTASSYVFILMCAFLPPFLLKPYGGILADRHDRRLM 77 Query: 62 MITGDLGSAVGLVFILVLMLEGEPSLGQICLGVALSSVFVSLLEPAYKATITDLLSVEQF 121 MI GDLGS +GL+FI +ML+G L QI LG+A+SSVF + EPAYKA ITDLL Q+ Sbjct: 78 MILGDLGSTLGLLFIFAMMLKGNIELWQIYLGIAISSVFSAFQEPAYKALITDLLPENQY 137 Query: 122 AKASGLVQLAASSKYLLSPVLAGSLLAXXXXXXXXXXXXXXXXXXXXXXXXXKRSMNTPK 181 AKASGL+QLA+S++YL+SP LAG +L ++ ++ + Sbjct: 138 AKASGLMQLASSAQYLISPFLAGIILTIMDIKFVFLIDVTTFLIASSIVIWIRKILSRTQ 197 Query: 182 SAAVTGVPLQLFKDLREGWKALASVQGVLQLIFVLSMVTFCAGFLQTLFTPLLLAFTDTR 241 +T DL+EG + + +G++ L+ +++ F G LQ+LF P++L T + Sbjct: 198 ---ITKQEQNNMADLKEGIQEFSKNRGIVNLVITTTLILFFVGLLQSLFIPMMLNLTTVK 254 Query: 242 TLGMIESISAVGMLAGSLVIGIFSLKGNIVRLLAVSLGVAGLCFALVGMSTNTALITSAG 301 +G I+SI A G+L GSL IG++ K V+ L++SL V+G+ FA +G+STN IT AG Sbjct: 255 AVGTIQSICASGILVGSLFIGVYGSKNKYVKTLSISLFVSGIFFANLGLSTNVIFITLAG 314 Query: 302 FLFFAALPFVNTSADVMIRSNIPGEVQGRAWGIIGVLSQFGYVAAYACSGVLSDQVFNPL 361 F+FF+ LPF+NT+ +V+IR NI QGR W II +++ G + A+A +G L+D++FNPL Sbjct: 315 FMFFSTLPFINTAIEVLIRKNIENSKQGRIWSIISMVTYLGSIIAFAVAGFLADKIFNPL 374 Query: 362 LQEGGML 368 L+ G+L Sbjct: 375 LESDGLL 381 >Q46AW2.1 Q46AW2_METBF Putative uncharacterized protein Length = 448 Score = 327 bits (838), Expect = 2e-87, Method: Compositional matrix adjust. Identities = 166/370 (44%), Positives = 239/370 (64%), Gaps = 3/370 (0%) Query: 2 GEWISSIGSGLTAFALGVYVFHMTGTATSVAMVTLCAYVPSIVLGPFGGVLADRFDRRLM 61 G++IS IG+GLT F+LGVYV+ T TA+S + +C ++P +L P+GG+LADR+DRRLM Sbjct: 25 GQFISVIGTGLTVFSLGVYVYQQTNTASSYVFILMCVFLPPFLLKPYGGILADRYDRRLM 84 Query: 62 MITGDLGSAVGLVFILVLMLEGEPSLGQICLGVALSSVFVSLLEPAYKATITDLLSVEQF 121 MI GDLG+ +GL+FI +ML+G L QI LG+A SSVF + EPAYKA ITDLL Q+ Sbjct: 85 MILGDLGATLGLLFIFFMMLKGNIQLWQIYLGIATSSVFSAFQEPAYKALITDLLPEAQY 144 Query: 122 AKASGLVQLAASSKYLLSPVLAGSLLAXXXXXXXXXXXXXXXXXXXXXXXXXKRSMNTPK 181 A+ASGLVQLA+S++YL+SP LAG LL + N Sbjct: 145 ARASGLVQLASSAQYLISPFLAGILLTIIDIKFIFLIDTTTLLISSSIVIWIR---NISG 201 Query: 182 SAAVTGVPLQLFKDLREGWKALASVQGVLQLIFVLSMVTFCAGFLQTLFTPLLLAFTDTR 241 + + DL+EG K ++ +GV+ L+ + + F G LQ+LF PLLL+ T + Sbjct: 202 KVKIDQHKQNIIADLKEGMKEFSNNRGVVNLVIITMITLFFVGLLQSLFIPLLLSLTTVK 261 Query: 242 TLGMIESISAVGMLAGSLVIGIFSLKGNIVRLLAVSLGVAGLCFALVGMSTNTALITSAG 301 T G+ +SI A G+L GSL IG+F K V+ L++SL ++G+ FA +G+S N +T AG Sbjct: 262 TAGISQSICASGILIGSLFIGVFGSKNRYVKTLSISLFLSGIFFANLGISKNILFVTLAG 321 Query: 302 FLFFAALPFVNTSADVMIRSNIPGEVQGRAWGIIGVLSQFGYVAAYACSGVLSDQVFNPL 361 F+FFA LPF+NTS +V+IR NI + QGR W +I +++ G + A+A +G L+D++FNPL Sbjct: 322 FMFFATLPFINTSIEVLIRKNIDNKKQGRVWSLISMITYLGSIMAFAVAGFLADKIFNPL 381 Query: 362 LQEGGMLASS 371 L+ G++ + Sbjct: 382 LEVDGLMVET 391 >Q5WLF5.1 Q5WLF5_BACSK Major facilitator (MFS) superfamily macrolide-efflux protein Length = 441 Score = 325 bits (833), Expect = 6e-87, Method: Compositional matrix adjust. Identities = 189/414 (45%), Positives = 260/414 (62%), Gaps = 7/414 (1%) Query: 1 MGEWISSIGSGLTAFALGVYVFHMTGTATSVAMVTLCAYVPSIVLGPFGGVLADRFDRRL 60 +GE IS+IG+GLTAFALGVYV+ MT +A VAMV + A +P I+L P GVLADRFDRRL Sbjct: 18 VGELISNIGTGLTAFALGVYVWLMTQSAVDVAMVEMAALLPMILLAPAAGVLADRFDRRL 77 Query: 61 MMITGDLGSAVGLVFILVLMLEGEPSLGQICLGVALSSVFVSLLEPAYKATITDLLSVEQ 120 +M+ GD+ S LV +LVL++ G + QIC+ + +SS+F +LL PAYKATITDLL+ E+ Sbjct: 78 LMMLGDIVSGFALVVLLVLIINGHIQVWQICVCIGVSSLFSALLNPAYKATITDLLTEEE 137 Query: 121 FAKASGLVQLAASSKYLLSPVLAGSLLAXXXXXXXXXXXXXXXXXXXXXXXXXKRSMN-T 179 +AKASG+V +A+SSK+L+SP+LAG +LA ++S+ Sbjct: 138 YAKASGMVGIASSSKFLISPILAGFILAFSTIEVVLIIDILTFVIAVIAIFLIRKSLAIK 197 Query: 180 PKSAAVTGVPLQLFKDLREGWKALASVQGVLQLIFVLSMVTFCAGFLQTLFTPLLLAFTD 239 P+ L F DL+EGW + +GVL+LI ++S + F G +Q L PLLLA TD Sbjct: 198 PRVKE----KLNFFNDLKEGWLIILESKGVLRLIVLVSFLMFYMGMIQVLSKPLLLAITD 253 Query: 240 TRTLGMIESISAVGMLAGSLVIGIFSLKGNIVRLLAVSLGVAGLCFALVGMSTNTALITS 299 +T G++ +I A GML S+VIG LK N V ++ S VAG+ A G +T+ +I Sbjct: 254 EKTTGILLTIIACGMLVSSIVIGNGILKKNYVNVMVGSFIVAGVTMAGFGATTSIPVILL 313 Query: 300 AGFLFFAALPFVNTSADVMIRSNIPGEVQGRAWGIIGVLSQFGYVAAYACSGVLSDQVFN 359 +GFLFFA+LPF N S DV+IR +I QGRAWG+I ++SQ G+V A+ G L+D VFN Sbjct: 314 SGFLFFASLPFANASIDVLIRKSIDNHKQGRAWGLISLISQVGFVVAFVIGGFLADHVFN 373 Query: 360 PLLQEGGMLASSVXXXXXXXXXXXXXXXXMVAG-GLIVFAAFMISGMTSIRSLN 412 P EGG+LAS+V ++AG GLI+ A F +S IR ++ Sbjct: 374 PAFAEGGVLASTVGKIIGTGEARGIGFLIILAGLGLIITALF-VSRSRGIRDMD 426 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.325 0.138 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,940,654,505 Number of extensions: 70813346 Number of successful extensions: 327375 Number of sequences better than 1.0e-05: 1303 Number of HSP's gapped: 327706 Number of HSP's successfully gapped: 1361 Length of query: 416 Length of database: 3,060,696,203 Length adjustment: 138 Effective length of query: 278 Effective length of database: 1,760,596,133 Effective search space: 489445724974 Effective search space used: 489445724974 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 132 (55.5 bits)