BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0334.0005.p (476 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value C5EE57.1 C5EE57_9FIRM Secreted sugar-binding protein 341 1e-91 C4EIT6.1 C4EIT6_STRRS Carbohydrate ABC transporter substrate-bin... 315 6e-84 C0T359.1 C0T359_9PSEU Carbohydrate ABC transporter substrate-bin... 314 2e-83 Q829N7.1 Q829N7_STRAW Putative secreted sugar-binding protein 297 2e-78 C1AY05.1 C1AY05_RHOOB Putative ABC transporter substrate-binding... 291 1e-76 >C5EE57.1 C5EE57_9FIRM Secreted sugar-binding protein Length = 450 Score = 341 bits (874), Expect = 1e-91, Method: Compositional matrix adjust. Identities = 177/415 (42%), Positives = 244/415 (58%), Gaps = 6/415 (1%) Query: 55 KSDNAADTAEHGGKVVLTYWDENAGPSRTPILQELLRRFEQKNPDIQVKYEGMPAGINKA 114 K+DN A G + +WDENAGP+RTP +EL++RFE++NPDI+++Y G+P K Sbjct: 34 KADNG--KAASGEVKTVVFWDENAGPNRTPYYEELIKRFEEQNPDIKIEYVGLPNSDAKN 91 Query: 115 KYDLAVASGDLPDAGGINGEWIADYAAKGVLLPLDAYFADWPERGQIDPSFIDYNRSLTQ 174 K ++AV PD G+ +W A + L PLDAYF W E +I+ S + R+L+ Sbjct: 92 KIEVAVTGNAAPDVCGMPPQWQAGFILNDALEPLDAYFDSWEEHDKINQSIVQSLRTLSV 151 Query: 175 DGKLYQIPSTYYLD-VFWYRSDWFAAAGLPRPQTWEDIFRSVRVLANTRQGSFGYSLRGG 233 D KLY IP+T + +FW R DWF G+ P TW+D + V + ++ G++G+S+RGG Sbjct: 152 DDKLYCIPNTSTFNPMFWGRKDWFEEKGVEAPGTWDDFYDLVELFTDSDAGTYGFSIRGG 211 Query: 234 EGSVAQLTSLMYAYSGITGSFRTDGSSTVNDPVHIEFLKRYLALYKAYTPASDIVSGYKE 293 G QL +YAYSGIT F DG ST+NDP ++E L R +Y YTP SDI +GYKE Sbjct: 212 AGGPMQLQEYLYAYSGITQPFTEDGKSTLNDPKNVEALDRLKDIYNKYTPESDISNGYKE 271 Query: 294 VVANFDSGRAAMIQHNLGSYQDHKKALGTGHFDGVLLPPSINGTRTLFGN--ANGYGIMR 351 +VA FD+G AAMIQHNLGS+ +H K LG G F P S G R + G NGY + + Sbjct: 272 MVAAFDTGSAAMIQHNLGSFGEHSKTLGEGKFFAFAPPVSATGKRVISGGYPVNGYVMFK 331 Query: 352 TTRHPDEAWRLIRYLLSEESQIYWNSQVGQIPTNLKAWNDPYFLSHPVLREAIEAFRSPD 411 ++ DEAWR I +L S ESQ YWN +GQ+PT+ N+ + ++ + EA++ + Sbjct: 332 QSQVKDEAWRFISFLCSAESQSYWNQNIGQVPTHTDLTNEDWVVNTQHINEAMKMQSDDN 391 Query: 412 TAFVRAPYELPDYPSLIRLI-EPEFQQVLAGTMDVEHFLNFWAQLMENSRRQHLH 465 T P LPDY S+ I P FQ VLAG E FL WA M + ++ Sbjct: 392 TIAFPYPLYLPDYQSIQDSIAAPGFQAVLAGQKSSEEFLTEWADAMTGAEAEYTQ 446 >C4EIT6.1 C4EIT6_STRRS Carbohydrate ABC transporter substrate-binding protein, CUT1 family Length = 438 Score = 315 bits (808), Expect = 6e-84, Method: Compositional matrix adjust. Identities = 150/438 (34%), Positives = 246/438 (56%), Gaps = 7/438 (1%) Query: 32 KSQRAACIFLIVVLGLLLSGCFPKSDNAADTAEHGGKVVLTYWDENAGPSRTPILQELLR 91 +S+ A + ++LG L+ C + D GGK +T+WD+N GP+RTP+ Q ++ Sbjct: 4 RSKHLAVLATALMLGTGLAAC-----GSDDAGSPGGKTTITFWDDNGGPARTPVWQHIIA 58 Query: 92 RFEQKNPDIQVKYEGMPAGINKAKYDLAVASGDLPDAGGINGEWIADYAAKGVLLPLDAY 151 F++ NP I VKY G+P + KYD A+A G LPD GG++ ++ A+ L PLD Sbjct: 59 EFQKANPTITVKYVGIPIAQAQQKYDTAIAGGGLPDVGGVSTAMLSSLVAQKALEPLDGR 118 Query: 152 FADWPERGQIDPSFIDYNRSLTQDGKLYQIPSTYYLDVFWYRSDWFAAAGLPRPQTWEDI 211 + G+++ + ++ DGK Y +P + + VFWYR+DWF AGL P+ W D Sbjct: 119 ISGGALNGRLNDQVVTSVKATVPDGKTYMVPMSTNMGVFWYRTDWFGEAGLEPPRNWGDF 178 Query: 212 FRSVRVLANTRQGSFGYSLRGGEGSVAQLTSLMYAYSGITGSFRTDGSSTVNDPVHIEFL 271 F + L +T + +G+++RGG GS+AQ+ ++Y SGIT F DG +TVNDP ++ L Sbjct: 179 FTATEKLTDTAKNRYGFTIRGGAGSIAQMLEVVYGQSGITEIFDADGKATVNDPKNVAAL 238 Query: 272 KRYLALYKAYTPASDIVSGYKEVVANFDSGRAAMIQHNLGSYQDHKKALGTGHFDGVLLP 331 ++ LYK TP +D+ + Y ++VA FD G A++QHNLGS+ DH K LG + +P Sbjct: 239 EKLAGLYKKVTPEADVSNDYVKMVAQFDGGNIAIMQHNLGSFNDHVKTLGKDKVAAMAVP 298 Query: 332 PSINGTRTLFGN-ANGYGIMRTTRHPDEAWRLIRYLLSEESQIYWNSQVGQIPTNLKAWN 390 S G + + N +G G+ + D A++ + S+ +W + G +P N + Sbjct: 299 KSDGGVQAILSNPVSGIGLFASGEKKDAAYKFAEFAASKAMNSHWAEKTGVLPANTEVNG 358 Query: 391 DPYFLSHPVLREAIEAFRSPDTAFVRAPYELPDYPSLIRL-IEPEFQQVLAGTMDVEHFL 449 + + P + EA++ P T V+ PY LP++ ++ + +EPEFQ+VL GT+ + FL Sbjct: 359 EAWIQGLPHIAEAVKVLNDPATKVVQMPYYLPEFNAITKTDMEPEFQKVLQGTLPAKDFL 418 Query: 450 NFWAQLMENSRRQHLHSN 467 + +A + ++ + N Sbjct: 419 DAFAAKLTEAQASYKQRN 436 >C0T359.1 C0T359_9PSEU Carbohydrate ABC transporter substrate-binding protein, CUT1 family Length = 399 Score = 314 bits (804), Expect = 2e-83, Method: Compositional matrix adjust. Identities = 155/396 (39%), Positives = 235/396 (59%), Gaps = 4/396 (1%) Query: 69 VVLTYWDENAGPSRTPILQELLRRFEQKNPDIQVKYEGMPAGINKAKYDLAVASGDLPDA 128 + +T+WD N GP RTPI +EL++RFE NPDI V Y G+P+ + KYD A+A G PD Sbjct: 1 MTITFWDNNGGPGRTPIYEELIKRFEAANPDIHVDYVGIPSSSVQQKYDTAIAGGGTPDV 60 Query: 129 GGINGEWIADYAAKGVLLPLDAYFADWPERGQIDPSFIDYNRSLTQDGKLYQIPSTYYLD 188 GG+ ++++ + L+PLD P +G++ PS ++ R D KLY +PS+ +D Sbjct: 61 GGVTTSYLSNMVGQEALVPLDEKVDGGPLKGKLLPSLVETVRQTAPDNKLYSVPSSGNMD 120 Query: 189 VFWYRSDWFAAAGLPRPQTWEDIFRSVRVLANTRQGSFGYSLRGGEGSVAQLTSLMYAYS 248 V WYR+DWFA G+ P+TW++ + L + +GY++RGG GSV QL + YAYS Sbjct: 121 VIWYRTDWFAEKGVQPPRTWDEFTAAAEKLTDASANRYGYTIRGGAGSVFQLLTEAYAYS 180 Query: 249 GITGSFRTDGSSTVNDPVHIEFLKRYLALYKAYTPASDIVSGYKEVVANFDSGRAAMIQH 308 G T SF G STV+ P + + +++ ALYK TP +D+ + Y ++VA F G AAM+ H Sbjct: 181 G-TDSFFEGGKSTVDAPGNADLVEKVAALYKKATPEADVNNNYTQMVAQFTGGSAAMMHH 239 Query: 309 NLGSYQDHKKALGTGHFDGVLLPPSINGTRTLFGN-ANGYGIMRTTRHPDEAWRLIRYLL 367 NLGSY D KALG + LP +G RT+ N +G+ + R + + D AW+ + +L Sbjct: 240 NLGSYGDVTKALGD-KVQALPLPVGPSGKRTVVPNPTDGFAVFRNSENQDAAWKFVEFLT 298 Query: 368 SEESQIYWNSQVGQIPTNLKAWNDPYFLSHPVLREAIEAFRSPDTAFVRAPYELPDYPSL 427 S ES YWN +VGQIP N + P+ + ++ A++ P T V AP LP Y S+ Sbjct: 299 SAESNSYWNEKVGQIPANTDVRSQPWIEQNKPVKMALDVLEDPATITVPAPVYLPQYSSI 358 Query: 428 IRL-IEPEFQQVLAGTMDVEHFLNFWAQLMENSRRQ 462 + EP++Q+VL G + + FL A+ + ++++ Sbjct: 359 TKADSEPQYQKVLLGQLGADEFLKAMAEKLTTAQQE 394 >Q829N7.1 Q829N7_STRAW Putative secreted sugar-binding protein Length = 445 Score = 297 bits (760), Expect = 2e-78, Method: Compositional matrix adjust. Identities = 156/440 (35%), Positives = 243/440 (55%), Gaps = 6/440 (1%) Query: 25 LKESFMKKSQRAACIFLIVVLGLLLSGCFPKSDNAA--DTAEHGGKVVLTYWDENAGPSR 82 +K S + + A + L VL L + C AA E GK +T+WD N G R Sbjct: 1 MKISIHRSRRAATAVALGSVLALTATACGDDGSGAAGDKGTEGSGKGEITFWDNNGG-VR 59 Query: 83 TPILQELLRRFEQKNPDIQVKYEGMPAGINKAKYDLAVASGDLPDAGGINGEWIADYAAK 142 T I ++++ FE+K PDI+V Y G+PA ++KYD A+ G LPD GG+ +A+ A + Sbjct: 60 TDIWKQIIADFEKKYPDIKVNYVGIPAASAQSKYDTAIQGGGLPDVGGVGTAMLAEVAVQ 119 Query: 143 GVLLPLDAYFADWPERGQIDPSFIDYNRSLTQDGKLYQIPSTYYLDVFWYRSDWFAAAGL 202 G L PLD+ G++ + +D +R+ LYQ+P++ WYR+D F +AGL Sbjct: 120 GALEPLDSRLKKSSLNGELSQNLLDSSRAAGGGDTLYQVPTSANNGTLWYRTDLFKSAGL 179 Query: 203 PRPQTWEDIFRSVRVLANTRQGSFGYSLRGGEGSVAQLTSLMYAYSGITGSFRTDGSSTV 262 P TW + + L + + FG+++RGGEGS+A +Y +GIT F +TV Sbjct: 180 DAPTTWSTFYEAADKLTDKGKNKFGFTIRGGEGSIAPALDAVYGQTGIT-EFWNGEKTTV 238 Query: 263 NDPVHIEFLKRYLALYKAYTPASDIVSGYKEVVANFDSGRAAMIQHNLGSYQDHKKALGT 322 NDP ++ L++Y+ALYK TP++D+ + + ++VA +DSG M+ HNLGSYQDH KALG Sbjct: 239 NDPKNVAALEKYIALYKKDTPSADVNNDFTKMVAQWDSGTIGMLSHNLGSYQDHLKALGA 298 Query: 323 GHFDGVLLPPSINGTRTLFGN-ANGYGIMRTTRHPDEAWRLIRYLLSEESQIYWNSQVGQ 381 G F G+ P +GTR N +G + +++++ AW+ I + +S +N GQ Sbjct: 299 GKFKGIPNPTRDDGTRVQISNPVDGMSVFKSSKNKAAAWKFIEFAVSSAENSKFNESAGQ 358 Query: 382 IPTNLKAWNDPYFLSHPVLREAIEAFRSPDTAFVRAPYELPDYPSLIRLI-EPEFQQVLA 440 +P N +A D + + A EA DT V+ PY LPD+ ++ + EP FQ++L Sbjct: 359 VPANTEAAKDTWVQQAEPTKLAAEALNGADTKIVQLPYYLPDWNTISKADNEPNFQKLLL 418 Query: 441 GTMDVEHFLNFWAQLMENSR 460 G M + FL+ A + ++ Sbjct: 419 GKMSAKEFLDTLADQLNKAQ 438 >C1AY05.1 C1AY05_RHOOB Putative ABC transporter substrate-binding protein Length = 448 Score = 291 bits (745), Expect = 1e-76, Method: Compositional matrix adjust. Identities = 147/420 (35%), Positives = 245/420 (58%), Gaps = 4/420 (0%) Query: 47 LLLSGCFPKSDNAADTAEHGGKVVLTYWDENAGPSRTPILQELLRRFEQKNPDIQVKYEG 106 L+L+ C ++ LT+WD NAGP RTP+ +EL+RRFE NP I ++Y G Sbjct: 26 LVLASCSSETSLPDGALGDPNNAQLTFWDNNAGPDRTPLYEELIRRFEAANPGIDIQYVG 85 Query: 107 MPAGINKAKYDLAVASGDLPDAGGINGEWIADYAAKGVLLPLDAYFADWPERGQIDPSFI 166 +P+ + KY A+A G PD G ++ ++A A+ ++ LD + A P+R + D + Sbjct: 86 LPSDSAQQKYQTALAGGSAPDLGIVSTAYLAPLVAQDAVISLDDFMAASPQRDEYDAGIL 145 Query: 167 DYNRSLTQDGKLYQIPSTYYLDVFWYRSDWFAAAGLPRPQTWEDIFRSVRVLANTRQGSF 226 + R L+ +P T WYR+DWFA AG+ P T+++ F + + L + G + Sbjct: 146 ESARESGGGEHLFGLPYTSNNATLWYRADWFAEAGIEPPDTYDEFFEAAKDLTDKDDGRY 205 Query: 227 GYSLRGGEGSVAQLTSLMYAYSGITGSFRTDGSSTVNDPVHIEFLKRYLALYKAYTPASD 286 G+++RGG GS+ L M+A +GI SF +G ST+N P ++ ++R+ ALY TP +D Sbjct: 206 GFTIRGGAGSIYPLLQYMFASTGIE-SFFENGESTLNRPEMVDAIERFAALYDVATPTAD 264 Query: 287 IVSGYKEVVANFDSGRAAMIQHNLGSYQDHKKALGTGHFDGVLLPPSINGTRTLFGNA-N 345 + +G+ ++VA+F G AM+QHNLGS +H+KALG V LP + +G RT+ + Sbjct: 265 VNNGFPQMVASFGGGSVAMMQHNLGSLSNHEKALGD-KAGAVALPRNEDGKRTVMTDPFP 323 Query: 346 GYGIMRTTRHPDEAWRLIRYLLSEESQIYWNSQVGQIPTNLKAWNDPYFLSHPVLREAIE 405 Y + ++++H AW+ + ++ S+ESQ YWN +VGQIP N A + P F S P ++ A+ Sbjct: 324 SYTVFKSSKHQAAAWKFLEFMTSQESQAYWNERVGQIPVNKAARSAPEFASMPSVQAALA 383 Query: 406 AFRSPDTAFVRAPYELPDYPSLIRL-IEPEFQQVLAGTMDVEHFLNFWAQLMENSRRQHL 464 A P T V P LPD+ ++++ + ++Q+VL G + + F + +A+ ++ S +++ Sbjct: 384 ALDDPKTVLVTPPDYLPDFGKIVQVQMLGQWQKVLIGQLSAQDFADDFAEKLDRSMAKYV 443 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.320 0.137 0.422 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 3,038,475,085 Number of extensions: 135805625 Number of successful extensions: 322677 Number of sequences better than 1.0e-05: 1145 Number of HSP's gapped: 322340 Number of HSP's successfully gapped: 1147 Length of query: 476 Length of database: 3,060,696,203 Length adjustment: 140 Effective length of query: 336 Effective length of database: 1,741,754,103 Effective search space: 585229378608 Effective search space used: 585229378608 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 133 (55.8 bits)