BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0334.0003.p (782 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value C5D3Y6.1 C5D3Y6_9BACI Histidine kinase 179 1e-42 C3AF24.1 C3AF24_BACMY Integral membrane sensor signal transducti... 130 4e-28 C2ZYG8.1 C2ZYG8_BACCE Integral membrane sensor signal transducti... 130 7e-28 C2Z9I7.1 C2Z9I7_BACCE Integral membrane sensor signal transducti... 129 9e-28 Q01SI4.1 Q01SI4_SOLUE Sensor protein EC=2.7.13.3 116 1e-23 >C5D3Y6.1 C5D3Y6_9BACI Histidine kinase Length = 693 Score = 179 bits (454), Expect = 1e-42, Method: Compositional matrix adjust. Identities = 140/524 (26%), Positives = 250/524 (47%), Gaps = 43/524 (8%) Query: 262 DRVEKNHALVLVAGLVTSLLPFVLLYALPNIWGDRYVIAPEYTLIGLVPLSVILLYILNK 321 DR+ +N +L+AGL+ S P V+L +P+ + + Y+L ++ + L Y+L K Sbjct: 180 DRI-RNQMYLLIAGLILSFAPVVILSLIPDAFFSFSGVPFHYSLSSIIVFPMTLSYLLTK 238 Query: 322 RSMLDMQLYLPRLFIHSVYFGGVFVLLALAHV-----------VRDPFGVSGLFIC-FVA 369 + ++D R + F + V+L+L ++ ++ L +C FV Sbjct: 239 QEVVDF-----REIFRKISFQLLAVILSLVAFNLLLAMFYKFNLKSAVLINTLLVCTFVV 293 Query: 370 GTWLYRNLVQRSKRHAEGRKKWLEHQTYKLSIQAASKRNIQDILSMMGEMLHHIVDVEGI 429 +++ L + + + + ++ + + Q + ++++ ++ +++H +DV G+ Sbjct: 294 YDLIHKGLEPLKMKKWDLKNQEIQKEKLLILQQLLNGKHLEYCAKLIADLIHKTIDVSGV 353 Query: 430 FMIWQEAQNKQPIFHGTEKYGDMVYVNPA-----TGQDNRLLDRSYWARNYDFEHVVPLA 484 MIW N I H T G ++ N A Q + +N+ F P+ Sbjct: 354 CMIWNN-DNIPMILHKT---GVFLHFNDAELLTYVHQSCHPEKMNINGKNFFF---YPM- 405 Query: 485 GGEENAGEGYLCVGPKRNQSMFSAEEKQLIEEVGHESVRLLLNAGLLSGIYKEFQHTKEE 544 E+ G++ +G K N ++F EE +LIE++ ++ L ++ L I K+ + T +E Sbjct: 406 -NMEDVTIGWVIIGEKTNATVFEKEEIKLIEKIRTDATELFSSSKSLQQIEKQLKETMKE 464 Query: 545 NAAYKRDVSGIRAANYLLLEAQQAERIRLSYRLHDHLLQNLIFLSRDLEELADQGTVNAR 604 + YK+ + LL+ Q+ E+ +LS LHD +LQNLI L LE L+ ++ Sbjct: 465 SQDYKQ-------FHLLLINEQEEEKKKLSVFLHDEVLQNLILLFNKLELLSKNKEMDNG 517 Query: 605 RMASWLKCLYTSQQEIRLLCDELYPHIVDKADLEASLHWLLRTVKEQSGLNVSLDYRWGG 664 + L S EIR +C ELYP IV+ LE SL+ L R + + + +DY Sbjct: 518 VFEDIKESLRNSIFEIREMCHELYPVIVEDLGLEKSLYSLKRKCQTNHNVIIEIDYNTNL 577 Query: 665 ETPPDSILKSNLFRIIRELVLNVQKHAEASQLDIRFIHIPAEGICCTVSDNGKGFDATAF 724 P S L +FR+I+ELV N KH+ + ++ + + + V D+G GF Sbjct: 578 RVIPAS-LSIQVFRMIKELVCNAIKHSSSKKVFVSVVE-TNNFLNIKVKDHGIGFKVP-- 633 Query: 725 SEQSSFMNGSHLGLISVSSQIGHLGGELDIQSRPGKGAVITIRL 768 + S +HLGLI++ ++ L G LDIQS PG G ++I L Sbjct: 634 NRLSELSQNNHLGLITIQKRVNQLKGTLDIQSEPGAGTCVSITL 677 >C3AF24.1 C3AF24_BACMY Integral membrane sensor signal transduction histidine kinase Length = 560 Score = 130 bits (328), Expect = 4e-28, Method: Compositional matrix adjust. Identities = 134/523 (25%), Positives = 248/523 (47%), Gaps = 45/523 (8%) Query: 266 KNHALVLVAGLVTSLLPFVLLYALPNIWGDRYVIAPEYTLIGLVPLSVILLYILNKRSML 325 K+ +L+ G++ S LP VLL +P + I Y L ++ + + Y+L K+ ++ Sbjct: 41 KSQLYILIFGIILSFLPIVLLNLIPGLLFGESKIPFTYCLPSIIIFPLTISYLLIKQEVI 100 Query: 326 DMQLYLPRLFIHSVYFGGVFVLLALAHVVRDPFGVSGLFICFVAGT---------WLYRN 376 + ++ + + + +++ F+ L + V +S I + G ++Y+ Sbjct: 101 EFEIRISKWLTNLLFYLLNFLFLNFS--VEQFIDLSQKEIFILCGLSVLSVVLSVYIYK- 157 Query: 377 LVQRSKRHAEGRKKWLEHQTYKLSI-QAASK-RNIQDILSMMGEMLHHIVDVEGIFMIWQ 434 L+ R+ KK + +K+ + Q SK ++ +++ ++G++++ + ++ G+ +IW Sbjct: 158 LILSLFRYL-NIKKDAQSGLHKIELFQTLSKEKHAKELAKLVGQLINQMFNINGVSIIW- 215 Query: 435 EAQNKQPIFHGTEKYG---DM----VYVNPATGQDNRLLDRSYWARNYDFE--HVVPLAG 485 N I+H + G DM ++ + N LL + FE H++ Sbjct: 216 ---NSNSIYHSLYETGIFQDMDKFELFNQLNQLEQNYLLKEGTTPIHIQFEKYHLICFPI 272 Query: 486 GEENAGEGYLCVGPKRNQSMFSAEEKQLIEEVGHESVRLLLNAGLLSGIYKEFQHTKEEN 545 +G + +G K + ++F +E +EEV S LLL+ L+ KE + K + Sbjct: 273 LIYQGMQGAIIIGEKNDYTLFKPQEINELEEVVINSANLLLSVNTLNSNKKEIRKAKVKA 332 Query: 546 AAYKRDVSGIRAANYLLLEAQQAERIRLSYRLHDHLLQNLIFLSRDLEELADQGTVNARR 605 +K+ NY ++++ + ER RLS LHD +LQ LI ++ L + + Sbjct: 333 QNWKQ-------FNYQIIQSLENERKRLSIFLHDEILQILILQLNEIRMLQTKRNFSIDI 385 Query: 606 MASWLKCLYTSQQEIRLLCDELYPHIVDKADLEASLHWLLRTVKEQSGLNVSLDYRWGGE 665 + S + L IR +C L+P IV+ LEASLH L R + + + + + + Sbjct: 386 LNSMDEQLEDIIYSIRKMCHNLHPIIVEDLGLEASLHTLKREFELNYRILIHIQFNCKLK 445 Query: 666 TPPDSILKSNLFRIIRELVLNVQKHAEASQLDIRFIHIPAEGIC--CTVSDNGKGFDA-T 722 + L+ ++RII+EL+ N KH++ + + I HI E C CTV D+G GF Sbjct: 446 ILSKN-LEIQIYRIIKELLNNAIKHSKGNNVQI---HIIEERGCYVCTVEDDGIGFCVPN 501 Query: 723 AFSEQSSFMNGSHLGLISVSSQIGHLGGELDIQSRPGKGAVIT 765 +FSE F +HLGL+++ QI L G L I S G ++T Sbjct: 502 SFSE---FSTSNHLGLMTIQRQISELNGNLRISSEKEHGTLVT 541 >C2ZYG8.1 C2ZYG8_BACCE Integral membrane sensor signal transduction histidine kinase Length = 670 Score = 130 bits (326), Expect = 7e-28, Method: Compositional matrix adjust. Identities = 130/524 (24%), Positives = 246/524 (46%), Gaps = 41/524 (7%) Query: 266 KNHALVLVAGLVTSLLPFVLLYALPNIWGDRYVIAPEYTLIGLVPLSVILLYILNKRSML 325 K+ +L+ G++ S LP VLL +P + I Y L ++ + + Y+L K+ ++ Sbjct: 151 KSQLYILIFGIILSFLPIVLLNLIPGLLFGESKIPFTYCLPSIIIFPLTISYLLIKQEVI 210 Query: 326 DMQLYLPRLFIHSVYFGGVFVLLALAHVVRDPFGVSGLFICFVAGT-------WLYRNLV 378 + ++ + + + +++ F+ + +FI ++Y+ L+ Sbjct: 211 EFEIRISKWLTNLLFYLLNFLFFNFSVEQFIDLSQKEIFILCSLSVLSVVLSVYIYK-LI 269 Query: 379 QRSKRHAEGRKKWLEHQTYKLSI-QAASK-RNIQDILSMMGEMLHHIVDVEGIFMIWQEA 436 R+ +K + +K+ + Q SK ++ +++ ++G++++ + ++ G+ +IW Sbjct: 270 LSLFRYLNIKKDA-QSGLHKIELFQTLSKEKHAKELAKLVGQLINQMFNINGVSVIW--- 325 Query: 437 QNKQPIFHG---TEKYGDM----VYVNPATGQDNRLLDRSYWARNYDFE--HVVPLAGGE 487 N I+H T + DM ++ + N LL + FE H++ Sbjct: 326 -NSNSIYHSLYETGIFQDMDKFELFNQLNQLEQNYLLKEGTTPIHIQFEKYHLICFPILI 384 Query: 488 ENAGEGYLCVGPKRNQSMFSAEEKQLIEEVGHESVRLLLNAGLLSGIYKEFQHTKEENAA 547 +G + +G K + ++F +E +EEV S LLL+ L+ KE + K + Sbjct: 385 YQGMQGAIIIGEKNDYTLFKPQEINELEEVVINSANLLLSVNTLNSNKKEIRKAKVKAQN 444 Query: 548 YKRDVSGIRAANYLLLEAQQAERIRLSYRLHDHLLQNLIFLSRDLEELADQGTVNARRMA 607 +K+ NY ++++ ++ER RLS LHD +LQ LI ++ L + + + Sbjct: 445 WKQ-------FNYQIIQSLESERKRLSIFLHDEILQILILQLNEIRMLQTKRNFSIDILN 497 Query: 608 SWLKCLYTSQQEIRLLCDELYPHIVDKADLEASLHWLLRTVKEQSGLNVSLDYRWGGETP 667 S + L IR +C L+P IV+ LEASLH L R + + + + + + Sbjct: 498 SMDEQLEDIIYSIRKMCHNLHPIIVEDLGLEASLHTLKREFELNYRILIHIQFNCKLKIL 557 Query: 668 PDSILKSNLFRIIRELVLNVQKHAEASQLDIRFIHIPAEGIC--CTVSDNGKGFDA-TAF 724 +I + ++RII+EL+ N KH++ + + I HI E C CTV D+G GF +F Sbjct: 558 SKNI-EIQIYRIIKELLNNAIKHSKGNNVQI---HIIEERGCYVCTVEDDGIGFCVPNSF 613 Query: 725 SEQSSFMNGSHLGLISVSSQIGHLGGELDIQSRPGKGAVITIRL 768 SE F +HLGL+++ QI L G L I S G ++T + Sbjct: 614 SE---FSTSNHLGLMTIQRQISELNGNLRISSEKEHGTLVTFTI 654 >C2Z9I7.1 C2Z9I7_BACCE Integral membrane sensor signal transduction histidine kinase Length = 713 Score = 129 bits (325), Expect = 9e-28, Method: Compositional matrix adjust. Identities = 130/524 (24%), Positives = 246/524 (46%), Gaps = 41/524 (7%) Query: 266 KNHALVLVAGLVTSLLPFVLLYALPNIWGDRYVIAPEYTLIGLVPLSVILLYILNKRSML 325 K+ +L+ G++ S LP VLL +P + I Y L ++ + + Y+L K+ ++ Sbjct: 194 KSQLYILIFGIILSFLPIVLLNLIPGLLFGESKIPFTYCLPSIIIFPLTISYLLIKQEVI 253 Query: 326 DMQLYLPRLFIHSVYFGGVFVLLALAHVVRDPFGVSGLFICFVAGT-------WLYRNLV 378 + ++ + + + +++ F+ + +FI ++Y+ L+ Sbjct: 254 EFEIRISKWLTNLLFYLLNFLFFNFSVEQFIDLSQKEIFILCSLSVLSVVLSVYIYK-LI 312 Query: 379 QRSKRHAEGRKKWLEHQTYKLSI-QAASK-RNIQDILSMMGEMLHHIVDVEGIFMIWQEA 436 R+ +K + +K+ + Q SK ++ +++ ++G++++ + ++ G+ +IW Sbjct: 313 LSLFRYLNIKKDA-QSGLHKIELFQTLSKEKHAKELAKLVGQLINQMFNINGVSVIW--- 368 Query: 437 QNKQPIFHGTEKYG---DM----VYVNPATGQDNRLLDRSYWARNYDFE--HVVPLAGGE 487 N I+H + G DM ++ + N LL + FE H++ Sbjct: 369 -NSNSIYHSLYETGIFQDMDKFELFNQLNQLEQNYLLKEGTTPIHIQFEKYHLICFPILI 427 Query: 488 ENAGEGYLCVGPKRNQSMFSAEEKQLIEEVGHESVRLLLNAGLLSGIYKEFQHTKEENAA 547 +G + +G K + ++F +E +EEV S LLL+ L+ KE + K + Sbjct: 428 YQGMQGAIIIGEKNDYTLFKPQEINELEEVVINSANLLLSVNTLNSNKKEIRKAKVKAQN 487 Query: 548 YKRDVSGIRAANYLLLEAQQAERIRLSYRLHDHLLQNLIFLSRDLEELADQGTVNARRMA 607 +K+ NY ++++ ++ER RLS LHD +LQ LI ++ L + + + Sbjct: 488 WKQ-------FNYQIIQSLESERKRLSIFLHDEILQILILQLNEIRMLQTKRNFSIDILN 540 Query: 608 SWLKCLYTSQQEIRLLCDELYPHIVDKADLEASLHWLLRTVKEQSGLNVSLDYRWGGETP 667 S + L IR +C L+P IV+ LEASLH L R + + + + + + Sbjct: 541 SMDEQLEDIIYSIRKMCHNLHPIIVEDLGLEASLHTLKREFELNYRILIHIQFNCKLKIL 600 Query: 668 PDSILKSNLFRIIRELVLNVQKHAEASQLDIRFIHIPAEGIC--CTVSDNGKGFDA-TAF 724 +I + ++RII+EL+ N KH++ + + I HI E C CTV D+G GF +F Sbjct: 601 SKNI-EIQIYRIIKELLNNAIKHSKGNNVQI---HIIEERGCYVCTVEDDGIGFCVPNSF 656 Query: 725 SEQSSFMNGSHLGLISVSSQIGHLGGELDIQSRPGKGAVITIRL 768 SE F +HLGL+++ QI L G L I S G ++T + Sbjct: 657 SE---FSTSNHLGLMTIQRQISELNGNLRISSEKEHGTLVTFTI 697 >Q01SI4.1 Q01SI4_SOLUE Sensor protein EC=2.7.13.3 Length = 449 Score = 116 bits (290), Expect = 1e-23, Method: Compositional matrix adjust. Identities = 68/211 (32%), Positives = 115/211 (54%), Gaps = 10/211 (4%) Query: 562 LLEAQQAERIRLSYRLHDHLLQNLIFLSRDLEELADQGTVNARRMASWLKCLYTSQQEIR 621 +LE Q+A R S+ LHD L Q+L + +L LA A R+A + + + +R Sbjct: 241 MLEDQEATARRFSHELHDELGQSLTAVKTNLTALAGTTEPQAPRLADCVHLVDEAIGNVR 300 Query: 622 LLCDELYPHIVDKADLEASLHWLLRTVKEQSGLNVSLDYRWGGETPPDSILKSNLFRIIR 681 + L P I+D LEA L WL ++G+ V ++ + G P D+ +++LFRI + Sbjct: 301 QMSQLLRPTILDDFGLEAGLRWLADGFSARTGIEVHVESTYSGRLPDDT--ETHLFRISQ 358 Query: 682 ELVLNVQKHAEASQLDIRFIHIPAEGICCTVSDNGKGFDATAFSEQSSFMNGSHLGLISV 741 E + N+ +HA A + ++ PAE IC ++SD+G+G A NG LG+I + Sbjct: 359 EALTNIARHAAARHVTVKLDRKPAE-ICLSISDDGRGIAAAP-------QNGRGLGMIGM 410 Query: 742 SSQIGHLGGELDIQSRPGKGAVITIRLRPHH 772 ++ GG+++++SRPG+G +I +R+ H Sbjct: 411 RARARSAGGDVNVRSRPGEGVLIEVRVPLKH 441 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.323 0.137 0.416 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 4,204,214,027 Number of extensions: 166610928 Number of successful extensions: 418422 Number of sequences better than 1.0e-05: 1659 Number of HSP's gapped: 417402 Number of HSP's successfully gapped: 1660 Length of query: 782 Length of database: 3,060,696,203 Length adjustment: 144 Effective length of query: 638 Effective length of database: 1,704,070,043 Effective search space: 1087196687434 Effective search space used: 1087196687434 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 135 (56.6 bits)