BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0333.0012.p (474 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KBP1.1 B3KBP1_9BACI Pyruvate kinase EC=2.7.1.40 840 0.0 B1DDX2.1 B1DDX2_9BACL Pyruvate kinase EC=2.7.1.40 743 0.0 Q2B2J1.1 Q2B2J1_9BACI Pyruvate kinase EC=2.7.1.40 655 0.0 A8VX19.1 A8VX19_9BACI Pyruvate kinase EC=2.7.1.40 646 0.0 Q9K844.1 Q9K844_BACHD Pyruvate kinase EC=2.7.1.40 644 0.0 >B3KBP1.1 B3KBP1_9BACI Pyruvate kinase EC=2.7.1.40 Length = 475 Score = 840 bits (2171), Expect = 0.0, Method: Compositional matrix adjust. Identities = 410/472 (86%), Positives = 444/472 (94%) Query: 1 MRKSKIVCTIGPASESLENIKKLILAGMNVARLNFSHGDFEEHGNRIKTIRQASQELGKT 60 MRK+KIVCTIGP+SESLEN KKLI+AGMNVARLNFSHGDFEEHGNRIK IRQA +EL KT Sbjct: 1 MRKTKIVCTIGPSSESLENTKKLIMAGMNVARLNFSHGDFEEHGNRIKNIRQACEELNKT 60 Query: 61 VAILLDTKGPEIRTGKLEVEPIELVQDEYLTLTTEEILGDQNRISVTYADLPSDVQVGST 120 VAILLDTKGPEIRTGKLEVEPIELVQDEY+TLTTEEILGD+NR+S+TY +LP DVQVGST Sbjct: 61 VAILLDTKGPEIRTGKLEVEPIELVQDEYITLTTEEILGDKNRLSITYKELPQDVQVGST 120 Query: 121 ILIDDGLIGLTVVDIQGTEIKTRIVNGGTIKSKKGVNVPGVAISLPGITEKDTNDIIFGI 180 ILIDDGLIGLTVV+IQGTEI+ RIVNGGTIKSKKGVNVPGVAISLPGITEKD NDIIFGI Sbjct: 121 ILIDDGLIGLTVVEIQGTEIRCRIVNGGTIKSKKGVNVPGVAISLPGITEKDANDIIFGI 180 Query: 181 EQDIDFIAASFVRKASDVQEIRALLEKHNASHIQIISKIENQQGVDNLDEILAVSDGLMV 240 EQ IDFIAASFVRKASDV EIR LL+KHN HIQIISKIENQQGVDNLDEIL VSDGLMV Sbjct: 181 EQGIDFIAASFVRKASDVLEIRELLKKHNGEHIQIISKIENQQGVDNLDEILEVSDGLMV 240 Query: 241 ARGDLGVEIPAEDVPLAQKLMIQKCNIAGKPVITATQMLDSMQRNPRPTRAEASDVANAI 300 ARGDLGVEIPAE+VPL QK MI+KCN+AGKPVITATQMLDSMQRNPRPTRAEASDVANAI Sbjct: 241 ARGDLGVEIPAEEVPLVQKRMIEKCNLAGKPVITATQMLDSMQRNPRPTRAEASDVANAI 300 Query: 301 FDGTDAIMLSGETAAGKYPVESVLTMSRIAEKAESALNHREIFMKQQIAQETTVTEAISQ 360 FDGTDAIMLSGETAAGKYPVESVLTMSRIAEKAESALN+RE+F+KQ+IAQET+VTEAISQ Sbjct: 301 FDGTDAIMLSGETAAGKYPVESVLTMSRIAEKAESALNYRELFLKQRIAQETSVTEAISQ 360 Query: 361 SVAISALDLNAKAIISSTVTGHTARVVSKYRPKSQIIAVTTQERTMRQLALVWGVTPVHG 420 SVAISALDLNAKAIISST +G TAR+VSKYRP++ I+AVTTQERT+R+LAL WGVTPV G Sbjct: 361 SVAISALDLNAKAIISSTESGQTARMVSKYRPQAPIVAVTTQERTLRRLALTWGVTPVKG 420 Query: 421 LEATSTDELLETALKGGKASGLVKAGDLVVITAGIPLGRSGSTNLVKVDTIP 472 +A+STDE+ + AL+GG SGLVK GDLVVITAG+PLGRSGSTNL+KVD IP Sbjct: 421 EQASSTDEMFDYALQGGVKSGLVKEGDLVVITAGVPLGRSGSTNLLKVDQIP 472 >B1DDX2.1 B1DDX2_9BACL Pyruvate kinase EC=2.7.1.40 Length = 584 Score = 743 bits (1917), Expect = 0.0, Method: Compositional matrix adjust. Identities = 365/468 (77%), Positives = 412/468 (88%) Query: 1 MRKSKIVCTIGPASESLENIKKLILAGMNVARLNFSHGDFEEHGNRIKTIRQASQELGKT 60 MRK+KIVCTIGP+SESLEN KKLI AGMNVARLNFSHGDFEEHGNRIK I A++ELG + Sbjct: 1 MRKAKIVCTIGPSSESLENTKKLIKAGMNVARLNFSHGDFEEHGNRIKNIGIANKELGTS 60 Query: 61 VAILLDTKGPEIRTGKLEVEPIELVQDEYLTLTTEEILGDQNRISVTYADLPSDVQVGST 120 VAILLDTKGPEIR GKL+ EPIELV E + LTTEEILGD NR+ +TY +LP+DV VGST Sbjct: 61 VAILLDTKGPEIRLGKLKEEPIELVAGERVALTTEEILGDINRVPITYDNLPNDVSVGST 120 Query: 121 ILIDDGLIGLTVVDIQGTEIKTRIVNGGTIKSKKGVNVPGVAISLPGITEKDTNDIIFGI 180 ILIDDGLIGLTV ++QGTEI RI N G IKSKKGVNVPGVAISLPGITEKD NDIIFGI Sbjct: 121 ILIDDGLIGLTVEEVQGTEIICRINNSGQIKSKKGVNVPGVAISLPGITEKDANDIIFGI 180 Query: 181 EQDIDFIAASFVRKASDVQEIRALLEKHNASHIQIISKIENQQGVDNLDEILAVSDGLMV 240 E IDF+AASFVRKASDV EIR LLE+HNASHIQIISKIENQQGVDNLDEIL VSDGLMV Sbjct: 181 EMGIDFVAASFVRKASDVLEIRELLERHNASHIQIISKIENQQGVDNLDEILEVSDGLMV 240 Query: 241 ARGDLGVEIPAEDVPLAQKLMIQKCNIAGKPVITATQMLDSMQRNPRPTRAEASDVANAI 300 ARGDLGVEIPAE+VPL QK MI+KCN AGKPVITATQMLDSMQRNPRPTRAEASDVANAI Sbjct: 241 ARGDLGVEIPAEEVPLVQKTMIEKCNRAGKPVITATQMLDSMQRNPRPTRAEASDVANAI 300 Query: 301 FDGTDAIMLSGETAAGKYPVESVLTMSRIAEKAESALNHREIFMKQQIAQETTVTEAISQ 360 FDGTDAIMLSGETAAGKYPVE+V TMSRIAE+AE+AL +REIF KQ AQ+T+VTEAISQ Sbjct: 301 FDGTDAIMLSGETAAGKYPVEAVTTMSRIAERAEAALEYREIFTKQANAQKTSVTEAISQ 360 Query: 361 SVAISALDLNAKAIISSTVTGHTARVVSKYRPKSQIIAVTTQERTMRQLALVWGVTPVHG 420 +VA+SALDLNA AI++ST +G TAR+VSKYRPK+ IIAVT E+ MR+LAL+WGV PV G Sbjct: 361 AVAVSALDLNAAAIVTSTQSGFTARMVSKYRPKAPIIAVTNDEKVMRRLALIWGVKPVLG 420 Query: 421 LEATSTDELLETALKGGKASGLVKAGDLVVITAGIPLGRSGSTNLVKV 468 A +TD + E A+ G +++GL+ GD +VITAG+P+GR+G+TNL+K+ Sbjct: 421 EIAETTDAMFENAVDGARSTGLISLGDTIVITAGVPVGRAGTTNLIKI 468 >Q2B2J1.1 Q2B2J1_9BACI Pyruvate kinase EC=2.7.1.40 Length = 586 Score = 655 bits (1691), Expect = 0.0, Method: Compositional matrix adjust. Identities = 322/473 (68%), Positives = 390/473 (82%), Gaps = 2/473 (0%) Query: 1 MRKSKIVCTIGPASESLENIKKLILAGMNVARLNFSHGDFEEHGNRIKTIRQASQELGKT 60 MRK+KIVCTIGPASES+E + +LI AGMNV+RLNFSHGDFEEHG RIK IR+AS+ GKT Sbjct: 1 MRKTKIVCTIGPASESIEKLTQLIEAGMNVSRLNFSHGDFEEHGARIKNIREASKSTGKT 60 Query: 61 VAILLDTKGPEIRTGKLEVEPIELVQDEYLTLTTEEILGDQNRISVTYADLPSDVQVGST 120 VAILLDTKGPEIRT +E +EL + ++ E+LG+ ++ SVTYA L DV GS Sbjct: 61 VAILLDTKGPEIRTNNMENGAVELEAGNNIIVSMNEVLGNADKFSVTYAGLIDDVHTGSK 120 Query: 121 ILIDDGLIGLTV--VDIQGTEIKTRIVNGGTIKSKKGVNVPGVAISLPGITEKDTNDIIF 178 IL+DDGLIGL V +D +EI T+I+N GT+K+KKGVNVPGV++ LPGITEKD NDI+F Sbjct: 121 ILLDDGLIGLEVTKIDKANSEIHTKILNSGTLKNKKGVNVPGVSVKLPGITEKDANDILF 180 Query: 179 GIEQDIDFIAASFVRKASDVQEIRALLEKHNASHIQIISKIENQQGVDNLDEILAVSDGL 238 GIEQ +DFIAASFVR+ASDV EIR LLE +NASHIQII KIENQ+GVDN+DEIL VSDGL Sbjct: 181 GIEQGVDFIAASFVRRASDVLEIRQLLEDNNASHIQIIPKIENQEGVDNIDEILEVSDGL 240 Query: 239 MVARGDLGVEIPAEDVPLAQKLMIQKCNIAGKPVITATQMLDSMQRNPRPTRAEASDVAN 298 MVARGDLGVEIPAE+VPL QK +I+KCNI GKPVITATQMLDSMQRNPRPTRAEASDVAN Sbjct: 241 MVARGDLGVEIPAEEVPLVQKSLIKKCNIQGKPVITATQMLDSMQRNPRPTRAEASDVAN 300 Query: 299 AIFDGTDAIMLSGETAAGKYPVESVLTMSRIAEKAESALNHREIFMKQQIAQETTVTEAI 358 AIFDGTDAIMLSGETAAG YPVE+V TM IA +AESAL+H+EI + E +T+AI Sbjct: 301 AIFDGTDAIMLSGETAAGTYPVEAVQTMHNIASRAESALDHKEILSNRSKDNEHNITDAI 360 Query: 359 SQSVAISALDLNAKAIISSTVTGHTARVVSKYRPKSQIIAVTTQERTMRQLALVWGVTPV 418 QSVA +AL+L+ AII+ T +GHTAR++SKYRPK+ I+AVT+QE R+L+LVWGV P Sbjct: 361 GQSVAHTALNLDVNAIITPTESGHTARMISKYRPKAPIVAVTSQESVTRRLSLVWGVYPQ 420 Query: 419 HGLEATSTDELLETALKGGKASGLVKAGDLVVITAGIPLGRSGSTNLVKVDTI 471 G EA++TD++L++A++ SGLV +GDLVVITAG+P+G +G+TNL+K+ I Sbjct: 421 VGQEASTTDDMLDSAVQESMNSGLVGSGDLVVITAGVPVGEAGTTNLMKIHVI 473 >A8VX19.1 A8VX19_9BACI Pyruvate kinase EC=2.7.1.40 Length = 536 Score = 646 bits (1666), Expect = 0.0, Method: Compositional matrix adjust. Identities = 319/471 (67%), Positives = 389/471 (82%) Query: 1 MRKSKIVCTIGPASESLENIKKLILAGMNVARLNFSHGDFEEHGNRIKTIRQASQELGKT 60 +RK+KIVCTIGPASES E + +LI AGMNVARLNFSHGDF+EHG RIK+IR+AS++ GK+ Sbjct: 3 IRKTKIVCTIGPASESPEKLTQLIDAGMNVARLNFSHGDFDEHGARIKSIREASEKAGKS 62 Query: 61 VAILLDTKGPEIRTGKLEVEPIELVQDEYLTLTTEEILGDQNRISVTYADLPSDVQVGST 120 VAILLDTKGPEIRT LE EL + + + ++ EE++G+ +RISVTY L +DV VGST Sbjct: 63 VAILLDTKGPEIRTQTLEGGIAELEKGKTVRVSMEEVVGNADRISVTYPGLVNDVHVGST 122 Query: 121 ILIDDGLIGLTVVDIQGTEIKTRIVNGGTIKSKKGVNVPGVAISLPGITEKDTNDIIFGI 180 +L+DDGLI L V I+ E+ T +VN GT+K+KKGVNVP V+++LPGITEKD NDI+FGI Sbjct: 123 LLLDDGLIELRVTAIEDKELVTEVVNSGTLKNKKGVNVPNVSVNLPGITEKDANDIVFGI 182 Query: 181 EQDIDFIAASFVRKASDVQEIRALLEKHNASHIQIISKIENQQGVDNLDEILAVSDGLMV 240 EQD+DFIAASFVR+ASDV EIR LLEKH A HIQII KIENQ+GVDN++EIL VSDGLMV Sbjct: 183 EQDVDFIAASFVRRASDVHEIRELLEKHGAEHIQIIPKIENQEGVDNIEEILEVSDGLMV 242 Query: 241 ARGDLGVEIPAEDVPLAQKLMIQKCNIAGKPVITATQMLDSMQRNPRPTRAEASDVANAI 300 ARGDLGVEIPAEDVPL QK +I+KCN GKPVITATQMLDSMQRNPRPTRAEASDVANAI Sbjct: 243 ARGDLGVEIPAEDVPLVQKDLIKKCNQIGKPVITATQMLDSMQRNPRPTRAEASDVANAI 302 Query: 301 FDGTDAIMLSGETAAGKYPVESVLTMSRIAEKAESALNHREIFMKQQIAQETTVTEAISQ 360 FDGTDAIMLSGETAAG YP ESV TM+ IA K ESAL +R+I K+ E TVT+AISQ Sbjct: 303 FDGTDAIMLSGETAAGDYPYESVTTMANIALKTESALKYRDILRKKSKEIENTVTDAISQ 362 Query: 361 SVAISALDLNAKAIISSTVTGHTARVVSKYRPKSQIIAVTTQERTMRQLALVWGVTPVHG 420 SV+ +AL+L+A AI+++T +GHTAR++SKYRP++ I+AVT+ R R LALVWGV P G Sbjct: 363 SVSHTALNLHASAIVTATQSGHTARMISKYRPEAPIVAVTSSSRVNRALALVWGVHPRTG 422 Query: 421 LEATSTDELLETALKGGKASGLVKAGDLVVITAGIPLGRSGSTNLVKVDTI 471 + STDE+L +++ + +GLV GDLVVITAG+P+G SG+TN++KV + Sbjct: 423 PQVNSTDEMLRLSVEEAQKTGLVNNGDLVVITAGVPVGESGTTNMMKVQLV 473 >Q9K844.1 Q9K844_BACHD Pyruvate kinase EC=2.7.1.40 Length = 584 Score = 644 bits (1662), Expect = 0.0, Method: Compositional matrix adjust. Identities = 318/471 (67%), Positives = 386/471 (81%) Query: 1 MRKSKIVCTIGPASESLENIKKLILAGMNVARLNFSHGDFEEHGNRIKTIRQASQELGKT 60 MRK+KIVCTIGPASES+E +++L+ AGMNVARLNFSHGDFEEHG RIK IR+A++ +GKT Sbjct: 1 MRKTKIVCTIGPASESVEKLEQLMEAGMNVARLNFSHGDFEEHGARIKNIREAAKRIGKT 60 Query: 61 VAILLDTKGPEIRTGKLEVEPIELVQDEYLTLTTEEILGDQNRISVTYADLPSDVQVGST 120 VAILLDTKGPEIRT LE EL + L ++ +E++G RISVTY L DV G+ Sbjct: 61 VAILLDTKGPEIRTQTLEGGVAELKAGQELVISMKEVVGTSERISVTYPGLVHDVVPGAK 120 Query: 121 ILIDDGLIGLTVVDIQGTEIKTRIVNGGTIKSKKGVNVPGVAISLPGITEKDTNDIIFGI 180 IL+DDGLIGL VV+++ EI T+++N GT+K+KKGVNVP V+++LPGITEKD DI FGI Sbjct: 121 ILLDDGLIGLEVVEVRDQEILTKVLNSGTLKNKKGVNVPNVSVNLPGITEKDAADIKFGI 180 Query: 181 EQDIDFIAASFVRKASDVQEIRALLEKHNASHIQIISKIENQQGVDNLDEILAVSDGLMV 240 EQ +DFIAASFVR+A DV EIR LLE+H A HI+II KIENQ+GVDN++EIL VSDGLMV Sbjct: 181 EQGVDFIAASFVRRAQDVLEIRELLEQHGAEHIKIIPKIENQEGVDNIEEILEVSDGLMV 240 Query: 241 ARGDLGVEIPAEDVPLAQKLMIQKCNIAGKPVITATQMLDSMQRNPRPTRAEASDVANAI 300 ARGDLGVEIPAE+VPL QK +I+KCN+ KPVITATQMLDSMQRNPRPTRAEASDVANAI Sbjct: 241 ARGDLGVEIPAEEVPLVQKSLIKKCNLVAKPVITATQMLDSMQRNPRPTRAEASDVANAI 300 Query: 301 FDGTDAIMLSGETAAGKYPVESVLTMSRIAEKAESALNHREIFMKQQIAQETTVTEAISQ 360 FDGTDAIMLSGETAAG YPVE+V TMSRIAE+AE ALN++EI K TT+T AISQ Sbjct: 301 FDGTDAIMLSGETAAGDYPVEAVQTMSRIAERAEQALNYQEILSKHTKEARTTITSAISQ 360 Query: 361 SVAISALDLNAKAIISSTVTGHTARVVSKYRPKSQIIAVTTQERTMRQLALVWGVTPVHG 420 SV +A +LNA AI+++T +GHTARVV+KYRPKS I+AVT+ E +RQLALVWGV + G Sbjct: 361 SVGHTAFNLNASAILTATESGHTARVVAKYRPKSPIVAVTSNETVVRQLALVWGVYSILG 420 Query: 421 LEATSTDELLETALKGGKASGLVKAGDLVVITAGIPLGRSGSTNLVKVDTI 471 EA +TD++L+ ++ S LVK GDLVVITAG+P+G +G+TN++KV I Sbjct: 421 EEAETTDDMLQVTVETALKSQLVKQGDLVVITAGVPVGETGTTNIMKVHVI 471 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.314 0.131 0.352 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,439,046,340 Number of extensions: 96813051 Number of successful extensions: 258092 Number of sequences better than 1.0e-05: 2627 Number of HSP's gapped: 250442 Number of HSP's successfully gapped: 2662 Length of query: 474 Length of database: 3,060,696,203 Length adjustment: 140 Effective length of query: 334 Effective length of database: 1,741,754,103 Effective search space: 581745870402 Effective search space used: 581745870402 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 133 (55.8 bits)