BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0333.0010.p (393 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Q8R7B7.1 Q8R7B7_THETN Permeases of the major facilitator superfa... 135 9e-30 B7R9U4.1 B7R9U4_9THEO Transporter, major facilitator family 132 5e-29 C0ZL72.1 C0ZL72_BREBN Probable maltose permease 124 1e-26 P39589.1 YWBF_BACSU Uncharacterized transporter ywbF 124 1e-26 B8CX10.1 B8CX10_HALOH Major facilitator superfamily MFS_1 118 1e-24 >Q8R7B7.1 Q8R7B7_THETN Permeases of the major facilitator superfamily Length = 382 Score = 135 bits (339), Expect = 9e-30, Method: Compositional matrix adjust. Identities = 90/300 (30%), Positives = 153/300 (51%), Gaps = 5/300 (1%) Query: 33 GLDSGRIGMLMATGTLIAICIQPLWGVISDRYNQARLVL-ILSVAVPAVLAVFYQSEYFI 91 G IG+LM+ G +AI QP WG+ SDR VL IL +A ++A+F S F Sbjct: 37 GYSKSEIGILMSLGPFVAIIAQPFWGITSDRARSKNFVLQILFLASIFIMALFPLSRNFY 96 Query: 92 VLLVVYTLSTIFSSTQAPIADSYAIVAANKAGSTYGSIRMMLSIGAAVGAYAGSQYVSHF 151 L+ V TL T F ++ PI+D+ + + G ++G IRM +IG AV + + Sbjct: 97 YLVAVITLFTFFQTSTGPISDAITLEYLSGTGQSFGPIRMAGTIGYAVMSVIAGWFAQR- 155 Query: 152 SVSTIWXXXXXXXXXXXXXXXTLPKQAEENRMMSQSFSKGIKTLLGNKIFLAFLGGSFLV 211 + +I+ LP + ++ + S I L N+ + + +FL+ Sbjct: 156 HIGSIFILNIIVMFIAFMIVYKLP-VVKGHQFGKEKIS--IWKLFSNRNLVVLMLLNFLI 212 Query: 212 NQTMTAFGTYFVLAFQSVGGSTSHAGIALFIASFTNFPSMLYASKVIKKLGRERTLLLGA 271 T+ + TYF + F+ +GGS G+A FI++ + P +LYA K++K++G L+ + Sbjct: 213 QTTLGFYYTYFPIYFRKIGGSNELLGLAYFISAMSEIPFLLYADKILKRIGTRWALIGAS 272 Query: 272 LIYVLRWGIQFAFPTPGVMIGVQVLHGLSFGLFYVAAVEYVSHITLPEMQATGQSVFNIV 331 + LRW I P +++ Q+LHGL F + Y + Y++ E++A+GQ+V N++ Sbjct: 273 GVAALRWLIISFIPNAYLVLPFQLLHGLIFIVLYYSMATYINQEVPKELKASGQTVNNLI 332 >B7R9U4.1 B7R9U4_9THEO Transporter, major facilitator family Length = 382 Score = 132 bits (333), Expect = 5e-29, Method: Compositional matrix adjust. Identities = 89/300 (29%), Positives = 151/300 (50%), Gaps = 5/300 (1%) Query: 33 GLDSGRIGMLMATGTLIAICIQPLWGVISDRYNQARLVL-ILSVAVPAVLAVFYQSEYFI 91 G IG+LM+ G + I QP WG+ SDR VL IL +A +A+F S F Sbjct: 37 GYSKSEIGILMSLGPFVVIIAQPFWGITSDRARSKNFVLQILFLASIFTMALFPLSRNFY 96 Query: 92 VLLVVYTLSTIFSSTQAPIADSYAIVAANKAGSTYGSIRMMLSIGAAVGAYAGSQYVSHF 151 L+ V TL T F ++ PI+D+ + + G ++G IRM +IG AV + + Sbjct: 97 YLIAVITLFTFFQTSTGPISDAITLEYLSGTGQSFGPIRMAGTIGYAVMSVIAGWFAQR- 155 Query: 152 SVSTIWXXXXXXXXXXXXXXXTLPKQAEENRMMSQSFSKGIKTLLGNKIFLAFLGGSFLV 211 + +I+ LP + ++ + S I L N+ + + +FL+ Sbjct: 156 HIGSIFILNIIVMFIAFMIVYKLP-VVKGHQFGKEKIS--IWKLFSNRNLVVLMLLNFLI 212 Query: 212 NQTMTAFGTYFVLAFQSVGGSTSHAGIALFIASFTNFPSMLYASKVIKKLGRERTLLLGA 271 T+ + TYF + F+ +GGS G+A FI++ + P +LYA K++K++G L+ + Sbjct: 213 QTTLGFYYTYFPIYFRKIGGSNELLGLAYFISAMSEIPFLLYADKILKRIGTRWALIGAS 272 Query: 272 LIYVLRWGIQFAFPTPGVMIGVQVLHGLSFGLFYVAAVEYVSHITLPEMQATGQSVFNIV 331 + LRW I P +++ Q+LHGL F + Y + Y++ E++A+GQ+V N++ Sbjct: 273 GVAALRWLIISFIPNAYLVLPFQLLHGLIFIVLYYSMATYINQEVPKELKASGQTVNNLI 332 >C0ZL72.1 C0ZL72_BREBN Probable maltose permease Length = 406 Score = 124 bits (312), Expect = 1e-26, Method: Compositional matrix adjust. Identities = 80/296 (27%), Positives = 145/296 (48%), Gaps = 1/296 (0%) Query: 34 LDSGRIGMLMATGTLIAICIQPLWGVISDRYNQARLVLILSVAVPAVLAVFYQS-EYFIV 92 L +IG++ A +++I QP+WG+I DRY + VLIL++ + ++ + + F+ Sbjct: 47 LTGTQIGLISAVAPIVSIVAQPMWGMICDRYQIRKTVLILTLIITGLVGLLFTGVSSFVY 106 Query: 93 LLVVYTLSTIFSSTQAPIADSYAIVAANKAGSTYGSIRMMLSIGAAVGAYAGSQYVSHFS 152 + ++Y L + F S PI+DS A+ AN G +G IR+ +IG A+ A+ V + Sbjct: 107 VFLLYILLSFFQSAVVPISDSLALGYANGHGIQFGDIRLWGAIGFALAAFITGLLVEQWG 166 Query: 153 VSTIWXXXXXXXXXXXXXXXTLPKQAEENRMMSQSFSKGIKTLLGNKIFLAFLGGSFLVN 212 ++ +P++ E S GI+ L F FL SF + Sbjct: 167 PHVLFYSFCLAFLIAILFLRKVPEEVTEASRFRVSMLAGIRNLARIPRFALFLISSFCMM 226 Query: 213 QTMTAFGTYFVLAFQSVGGSTSHAGIALFIASFTNFPSMLYASKVIKKLGRERTLLLGAL 272 ++ A +F L +Q +GG+ + G+A + + + P M A ++++ G E TLLL A+ Sbjct: 227 GSVNANNIWFSLFYQDIGGTIAGIGLAFLLFAGSEAPFMKLAGYIVRRWGLELTLLLSAI 286 Query: 273 IYVLRWGIQFAFPTPGVMIGVQVLHGLSFGLFYVAAVEYVSHITLPEMQATGQSVF 328 +RW + P+ +I + + G+S G + A ++V T +Q T ++F Sbjct: 287 FCTMRWFWYSSAPSTTAVIAMFFVQGISVGFYLATAAQFVRENTPASLQVTALAIF 342 >P39589.1 YWBF_BACSU Uncharacterized transporter ywbF Length = 399 Score = 124 bits (312), Expect = 1e-26, Method: Compositional matrix adjust. Identities = 81/311 (26%), Positives = 149/311 (47%), Gaps = 5/311 (1%) Query: 28 LLKQGGLDSGRIGMLMATGTLIAICIQPLWGVISDRYNQARLVLILSVAVPAVLAVFYQS 87 L K+ L ++G++M+ G ++ I QP WG++SD + + +L + ++ ++ + Y + Sbjct: 35 LTKEQHLSGSQVGLIMSLGPIVMIFFQPFWGMLSDYTQKTKGLLAVCTSITGIIGLAYIA 94 Query: 88 -EYFIVLLVVYTLSTIFSSTQAPIADSYAIVAANKAGSTYGSIRMMLSIGAAVGAYAGSQ 146 + F + +++ F ST P++DS ++ + YG IR+ S+G V +A Q Sbjct: 95 FDSFPLFILIAACFAAFQSTIIPLSDSISLRYTQETNGNYGGIRLFGSLGFGVAVFAMGQ 154 Query: 147 YVSH-FSVSTIWXXXXXXXXXXXXXXXTLPKQAEENRMMSQSFSKGIKTLLGNKIFLAFL 205 + + + I+ +P Q + ++ + KG + L+ NK FL F+ Sbjct: 155 VTNQLYPIHVIFIFGCAFLCIAAILASQVPGQQKTTKV---NIRKGFRELISNKTFLIFM 211 Query: 206 GGSFLVNQTMTAFGTYFVLAFQSVGGSTSHAGIALFIASFTNFPSMLYASKVIKKLGRER 265 +F A TYF L G S S GI FI + P M +A I K+G Sbjct: 212 IITFTTFAPNLANNTYFSLFLDKSGASLSAIGILFFIGVISEIPFMRFAQTFIDKMGLLN 271 Query: 266 TLLLGALIYVLRWGIQFAFPTPGVMIGVQVLHGLSFGLFYVAAVEYVSHITLPEMQATGQ 325 ++L + + RW + F P+ ++ L G++ GLF AA++YV IT ++AT Sbjct: 272 VIMLSGGVSLFRWALYFTAPSLWIIYATVFLQGVAIGLFIPAALQYVKKITPRHVEATAL 331 Query: 326 SVFNIVFSGFA 336 +++ + +GF Sbjct: 332 TMYAAIGNGFG 342 >B8CX10.1 B8CX10_HALOH Major facilitator superfamily MFS_1 Length = 378 Score = 118 bits (295), Expect = 1e-24, Method: Compositional matrix adjust. Identities = 80/301 (26%), Positives = 147/301 (48%), Gaps = 4/301 (1%) Query: 29 LKQGGLDSGRIGMLMATGTLIAICIQPLWGVISDRYNQARLVLILSVAVPAVLA-VFYQS 87 K+ G +G+IG++ + I++ + PLWG+ SD +N + +LI ++ +L VF + Sbjct: 24 FKKIGFTTGQIGLIQSVPAAISLLVMPLWGMASDYFNSNKRLLIYAITGTIILNLVFLTT 83 Query: 88 EYFIVLLVVYTLSTIFSSTQAPIADSYAIVAANKAGSTYGSIRMMLSIGAAVGAYAGSQY 147 EY+ + ++ IF P+AD+ + K G+ YG R+ SIG + + Y Sbjct: 84 EYYWYIFIIMVFFVIFQKPVIPLADALLLNYLGKRGNLYGRYRVWGSIGFTITVWLIGYY 143 Query: 148 VSHFSVSTIWXXXXXXXXXXXXXXXTLPKQAEENRMMSQSFSKGIKTLLGNKIFLAFLGG 207 + + + + ++ +P+ E R+ K LL N FL Sbjct: 144 LENTNPANLFYINAVALLVALLFILKVPEGKEAIRVNRL---KEFTGLLKNMDLFYFLLF 200 Query: 208 SFLVNQTMTAFGTYFVLAFQSVGGSTSHAGIALFIASFTNFPSMLYASKVIKKLGRERTL 267 +F + T+ + T+F L G S G+AL + + + +Y+ + +K + Sbjct: 201 TFFIQLTLFSNYTFFPLYVLDNGSRESLIGLALTVGATSEMFIFVYSDNIFRKFKIKSIF 260 Query: 268 LLGALIYVLRWGIQFAFPTPGVMIGVQVLHGLSFGLFYVAAVEYVSHITLPEMQATGQSV 327 ++ ++ + LRW + FP V IG Q+LH L+FGLF+V AV Y++ I + +ATGQ++ Sbjct: 261 MISSIAFTLRWFLLAYFPVSSVFIGSQLLHSLTFGLFHVTAVNYINIICGEDFKATGQNL 320 Query: 328 F 328 + Sbjct: 321 Y 321 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.326 0.138 0.396 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,764,210,175 Number of extensions: 62623229 Number of successful extensions: 239599 Number of sequences better than 1.0e-05: 288 Number of HSP's gapped: 239876 Number of HSP's successfully gapped: 290 Length of query: 393 Length of database: 3,060,696,203 Length adjustment: 138 Effective length of query: 255 Effective length of database: 1,760,596,133 Effective search space: 448952013915 Effective search space used: 448952013915 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 132 (55.5 bits)