BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0333.0007.p (40 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KBP3.1 B3KBP3_9BACI FxsA cytoplasmic membrane protein 67 5e-10 >B3KBP3.1 B3KBP3_9BACI FxsA cytoplasmic membrane protein Length = 130 Score = 67.0 bits (162), Expect = 5e-10, Method: Compositional matrix adjust. Identities = 28/40 (70%), Positives = 35/40 (87%) Query: 1 VTDIVGFSLVFPLTRPLYRVFLLKWIEKKMKNGTFTFYRR 40 VTDI+GF+L+FPLTRPLYR+ LLKW++K MKNG T +RR Sbjct: 91 VTDIIGFTLIFPLTRPLYRILLLKWMKKNMKNGKITIFRR 130 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.335 0.149 0.477 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 260,558,256 Number of extensions: 6002087 Number of successful extensions: 18816 Number of sequences better than 1.0e-05: 1 Number of HSP's gapped: 18816 Number of HSP's successfully gapped: 1 Length of query: 40 Length of database: 3,060,696,203 Length adjustment: 14 Effective length of query: 26 Effective length of database: 2,928,801,993 Effective search space: 76148851818 Effective search space used: 76148851818 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 39 (21.6 bits) S2: 125 (52.8 bits)