BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0333.0006.p (371 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KBP4.1 B3KBP4_9BACI Sporulation integral membrane protein YtvI 339 2e-91 C1P9T6.1 C1P9T6_BACCO Sporulation integral membrane protein YtvI 174 2e-41 C2WTQ3.1 C2WTQ3_BACCE Sporulation integral membrane protein YtvI 168 8e-40 Q812P4.1 Q812P4_BACCR Hypothetical Membrane Spanning Protein 167 2e-39 B7HFB0.1 B7HFB0_BACC4 Sporulation integral membrane protein YtvI 167 2e-39 >B3KBP4.1 B3KBP4_9BACI Sporulation integral membrane protein YtvI Length = 372 Score = 339 bits (870), Expect = 2e-91, Method: Compositional matrix adjust. Identities = 168/327 (51%), Positives = 228/327 (69%), Gaps = 1/327 (0%) Query: 46 MHPLV-LILRGFKLPRWLAVSLSLLFYVGGTALVLTALITRLVKELIGLLQTFDLHTDQW 104 M+P V L+ R ++PRW+AV+LSLL Y G L+LTA ++RLVKELI L Q+F+LH D W Sbjct: 46 MNPCVNLLQRSLRMPRWIAVTLSLLLYFGAALLILTAAVSRLVKELIALSQSFNLHIDAW 105 Query: 105 RELLLSISRNASXXXXXXXXXXFYHDNPDYHATIDSNISRTTETVGQAVTELITGFFNMV 164 E L + ++N S FY +NP+YH TI+ NI+RTT+T+G AVT+L+TG FN + Sbjct: 106 VEWLTAWTQNESFQNIVNEISRFYQNNPNYHDTINQNITRTTQTIGTAVTDLVTGIFNGI 165 Query: 165 LKLISALPSMGSILIVIMLSAFFLSTGWERHNAKLRALVPVPLRRPVSEIWHDLRNALFG 224 L++I LP++G+I IV++LS FFLS WERHN L L+P +R+P++ I+ DL+ ALFG Sbjct: 166 LQIIYYLPNLGTIFIVVLLSTFFLSNSWERHNRALLRLLPDVIRKPMNYIFSDLKKALFG 225 Query: 225 YLRAQLVLISXXXXXXXXXXXXXXXNSAFAIGLTIGLVDLVPYLGVGIVLLPWAIYSYMT 284 + RAQL+LIS +SAF+IGL IGLVDL+PYLGVGIVL+PW++Y++M+ Sbjct: 226 FARAQLILISITAVIVTITLYALGVDSAFSIGLLIGLVDLLPYLGVGIVLIPWSLYAFMS 285 Query: 285 GNLALGIGLSVLYAVILITRQILEPKVLASSIGLDPLAMLIGVFAXXXXXXXXXXXXXXX 344 +L LGIGL+VLY++IL+ RQI+EPKVLASS+GLDPLA L+G+F Sbjct: 286 DDLTLGIGLTVLYSIILVVRQIMEPKVLASSVGLDPLAALLGMFVGLKLFGILGLIIGPV 345 Query: 345 XXXXXDAFHRAGVFRALHSYIVSGRLH 371 DAFHRA VF+ L +YI+ GR Sbjct: 346 SLVILDAFHRAHVFKDLRNYIIGGRFR 372 >C1P9T6.1 C1P9T6_BACCO Sporulation integral membrane protein YtvI Length = 372 Score = 174 bits (440), Expect = 2e-41, Method: Compositional matrix adjust. Identities = 109/329 (33%), Positives = 168/329 (51%), Gaps = 9/329 (2%) Query: 46 MHPLVLIL-RGFKLPRWLAVSLSLLFYVGGTALVLTALITRLVKELIG----LLQTFDLH 100 ++PLV L R +LPR LAV +SL+ +G V +IT LV E++ L H Sbjct: 46 INPLVNFLDRKVRLPRALAVLISLILIIG----VFVGIITLLVAEIVTGANYLADVLPEH 101 Query: 101 TDQWRELLLSISRNASXXXXXXXXXXFYHDNPDYHATIDSNISRTTETVGQAVTELITGF 160 S+ F + + TI NI + + + + ++G Sbjct: 102 VQTLVNYSESLVTAQVLPLYDQLAGLFKNLDAGQQETIVQNIQAAGQKIASSAGDFLSGL 161 Query: 161 FNMVLKLISALPSMGSILIVIMLSAFFLSTGWERHNAKLRALVPVPLRRPVSEIWHDLRN 220 F + L+S +P+ ++LI +L+ FF+S W + K + L+P R+ ++ DLRN Sbjct: 162 FKKLPGLVSWIPNAATVLIFALLATFFISKDWNQLTGKGKLLLPKKARKSGITVFADLRN 221 Query: 221 ALFGYLRAQLVLISXXXXXXXXXXXXXXXNSAFAIGLTIGLVDLVPYLGVGIVLLPWAIY 280 ALFG++RA+L LIS + A I L G+VD++PYLG G+V +PW IY Sbjct: 222 ALFGFVRAELTLISMTLVIVLIGLLILRVDYAITIALVCGIVDILPYLGTGVVFVPWIIY 281 Query: 281 SYMTGNLALGIGLSVLYAVILITRQILEPKVLASSIGLDPLAMLIGVFAXXXXXXXXXXX 340 ++TG+L L IGL+VLY V+++ RQI+EPK+L+SSIG++PLA LI +F Sbjct: 282 EFITGSLPLAIGLTVLYIVVIVQRQIMEPKILSSSIGMNPLATLIALFIGFKTVGFLGLI 341 Query: 341 XXXXXXXXXDAFHRAGVFRALHSYIVSGR 369 A RA VF + +YI+ + Sbjct: 342 LGPITLVLLTALIRAHVFHDIWNYIMGKK 370 >C2WTQ3.1 C2WTQ3_BACCE Sporulation integral membrane protein YtvI Length = 365 Score = 168 bits (425), Expect = 8e-40, Method: Compositional matrix adjust. Identities = 107/321 (33%), Positives = 156/321 (48%), Gaps = 1/321 (0%) Query: 46 MHPLVLIL-RGFKLPRWLAVSLSLLFYVGGTALVLTALITRLVKELIGLLQTFDLHTDQW 104 ++P+V L + + PR LAV +SL+ G ++T L+T + LLQ + Sbjct: 39 INPVVNFLNQKLQFPRALAVLVSLILVFGAIVGLVTYLVTEAISATTYLLQLVTVKFPDI 98 Query: 105 RELLLSISRNASXXXXXXXXXXFYHDNPDYHATIDSNISRTTETVGQAVTELITGFFNMV 164 + N F H TI NI + EL+T +++ Sbjct: 99 VTFAQQFALNHIMPLYDDLISKFNHLGEPQRYTITQNIQNLGTEATTQMKELLTAIISVL 158 Query: 165 LKLISALPSMGSILIVIMLSAFFLSTGWERHNAKLRALVPVPLRRPVSEIWHDLRNALFG 224 ISALP+ ++L+ I+L+ FF+S W R K R L+P + I+ DLR ALFG Sbjct: 159 TNFISALPTTLTVLVFILLATFFISYDWHRLAQKARKLLPNRVHGYGKTIFVDLRKALFG 218 Query: 225 YLRAQLVLISXXXXXXXXXXXXXXXNSAFAIGLTIGLVDLVPYLGVGIVLLPWAIYSYMT 284 +++AQL L+S A I + G+VDL+PYLG G V +PW IY + T Sbjct: 219 FVKAQLTLVSMTTVIVLIGLLILRVPYAITIAIITGVVDLLPYLGTGAVFVPWVIYVFFT 278 Query: 285 GNLALGIGLSVLYAVILITRQILEPKVLASSIGLDPLAMLIGVFAXXXXXXXXXXXXXXX 344 G+ A IGL +LY V+++ RQI+EPKVL+S+IGLDPLA L+ +F Sbjct: 279 GDTAFAIGLLILYIVVIVQRQIMEPKVLSSNIGLDPLATLVALFVGFKLFGFLGLIIGPV 338 Query: 345 XXXXXDAFHRAGVFRALHSYI 365 + H+A VF L YI Sbjct: 339 TLVLLNTLHKANVFHDLWKYI 359 >Q812P4.1 Q812P4_BACCR Hypothetical Membrane Spanning Protein Length = 372 Score = 167 bits (422), Expect = 2e-39, Method: Compositional matrix adjust. Identities = 107/321 (33%), Positives = 155/321 (48%), Gaps = 1/321 (0%) Query: 46 MHPLVLIL-RGFKLPRWLAVSLSLLFYVGGTALVLTALITRLVKELIGLLQTFDLHTDQW 104 ++P+V L + + PR LAV +SL+ G ++T L+T + LLQ + Sbjct: 46 INPVVNFLNQKLQFPRALAVLVSLILVFGAIVGLVTYLVTEAISATTYLLQLVTVKFPDI 105 Query: 105 RELLLSISRNASXXXXXXXXXXFYHDNPDYHATIDSNISRTTETVGQAVTELITGFFNMV 164 + N F H TI NI + EL+T + + Sbjct: 106 VTFAQQFALNHIMPLYDDLISKFNHLGEPQRYTITQNIQNLGTEATTQMKELLTAIISGL 165 Query: 165 LKLISALPSMGSILIVIMLSAFFLSTGWERHNAKLRALVPVPLRRPVSEIWHDLRNALFG 224 ISALP+ ++L+ I+L+ FF+S W R K R L+P + I+ DLR ALFG Sbjct: 166 TNFISALPTTLTVLVFILLATFFISYDWHRLAQKARKLLPNRVHGYGKTIFVDLRKALFG 225 Query: 225 YLRAQLVLISXXXXXXXXXXXXXXXNSAFAIGLTIGLVDLVPYLGVGIVLLPWAIYSYMT 284 +++AQL L+S A I + G+VDL+PYLG G V +PW IY + T Sbjct: 226 FVKAQLTLVSMTTVIVLIGLLILRVPYAITIAIITGVVDLLPYLGTGAVFVPWVIYVFFT 285 Query: 285 GNLALGIGLSVLYAVILITRQILEPKVLASSIGLDPLAMLIGVFAXXXXXXXXXXXXXXX 344 G+ A IGL +LY V+++ RQI+EPKVL+S+IGLDPLA L+ +F Sbjct: 286 GDTAFAIGLLILYIVVIVQRQIMEPKVLSSNIGLDPLATLVALFVGFKLFGFLGLIIGPV 345 Query: 345 XXXXXDAFHRAGVFRALHSYI 365 + H+A VF L YI Sbjct: 346 TLVLLNTLHKANVFHDLWKYI 366 >B7HFB0.1 B7HFB0_BACC4 Sporulation integral membrane protein YtvI Length = 372 Score = 167 bits (422), Expect = 2e-39, Method: Compositional matrix adjust. Identities = 107/321 (33%), Positives = 155/321 (48%), Gaps = 1/321 (0%) Query: 46 MHPLVLIL-RGFKLPRWLAVSLSLLFYVGGTALVLTALITRLVKELIGLLQTFDLHTDQW 104 ++P+V L + + PR LAV +SL+ G ++T L+T + LLQ + Sbjct: 46 INPVVNFLNQKLQFPRALAVLVSLILVFGAIVGLVTYLVTEAISATTYLLQLVTVKFPDI 105 Query: 105 RELLLSISRNASXXXXXXXXXXFYHDNPDYHATIDSNISRTTETVGQAVTELITGFFNMV 164 + N F H TI NI + EL+T + + Sbjct: 106 VTFAQQFALNHIMPLYDDLISKFNHLGEPQRYTITQNIQNLGTEATTQMKELLTAIISGL 165 Query: 165 LKLISALPSMGSILIVIMLSAFFLSTGWERHNAKLRALVPVPLRRPVSEIWHDLRNALFG 224 ISALP+ ++L+ I+L+ FF+S W R K R L+P + I+ DLR ALFG Sbjct: 166 TNFISALPTTLTVLVFILLATFFISYDWHRLAQKARKLLPNRVHGYGKTIFVDLRKALFG 225 Query: 225 YLRAQLVLISXXXXXXXXXXXXXXXNSAFAIGLTIGLVDLVPYLGVGIVLLPWAIYSYMT 284 +++AQL L+S A I + G+VDL+PYLG G V +PW IY + T Sbjct: 226 FVKAQLTLVSMTTVIVLIGLLILRVPYAITIAIITGVVDLLPYLGTGAVFVPWVIYVFFT 285 Query: 285 GNLALGIGLSVLYAVILITRQILEPKVLASSIGLDPLAMLIGVFAXXXXXXXXXXXXXXX 344 G+ A IGL +LY V+++ RQI+EPKVL+S+IGLDPLA L+ +F Sbjct: 286 GDTAFAIGLLILYIVVIVQRQIMEPKVLSSNIGLDPLATLVALFVGFKLFGFLGLIIGPV 345 Query: 345 XXXXXDAFHRAGVFRALHSYI 365 + H+A VF L YI Sbjct: 346 TLVLLNTLHKANVFHDLWKYI 366 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.328 0.143 0.424 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,556,799,996 Number of extensions: 52189069 Number of successful extensions: 208249 Number of sequences better than 1.0e-05: 608 Number of HSP's gapped: 208357 Number of HSP's successfully gapped: 608 Length of query: 371 Length of database: 3,060,696,203 Length adjustment: 137 Effective length of query: 234 Effective length of database: 1,770,017,148 Effective search space: 414184012632 Effective search space used: 414184012632 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 132 (55.5 bits)