BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0333.0004.p (370 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KBP5.1 B3KBP5_9BACI 2-methylcitrate synthase/citrate synthase ... 555 e-156 B1DDW7.1 B1DDW7_9BACL 2-methylcitrate synthase/citrate synthase ... 496 e-138 Q5KWB5.1 Q5KWB5_GEOKA Citrate synthaseII EC=2.3.3.1 459 e-127 C3IXS6.1 C3IXS6_9BACI 2-methylcitrate synthase/citrate synthase ... 459 e-127 B7YAF8.1 B7YAF8_9BACI 2-methylcitrate synthase/citrate synthase ... 459 e-127 >B3KBP5.1 B3KBP5_9BACI 2-methylcitrate synthase/citrate synthase II EC=2.3.3.1 Length = 370 Score = 555 bits (1429), Expect = e-156, Method: Compositional matrix adjust. Identities = 266/370 (71%), Positives = 298/370 (80%) Query: 1 MTATKGLEGIVAXXXXXXXXVDGVLTYRGYDIDDLAEHATFEETAYLLWFGNLPTTPELQ 60 MTATKGLEGIVA VDGVLTYRGYDIDDLAEHA+FEE AYLLWFG LP EL+ Sbjct: 1 MTATKGLEGIVATTSAISSIVDGVLTYRGYDIDDLAEHASFEEVAYLLWFGKLPNVEELK 60 Query: 61 ALQRDLSAFAPIPEQVIAQMKLYPKEANTMXXXXXXXXXXXXYDEAADDMSREANEIKAV 120 ALQ+DLS +A IP+++I Q+KLYPK+ NTM YD DDMSREANE KAV Sbjct: 61 ALQQDLSNYAAIPDELIQQIKLYPKDGNTMAALRSAVSALALYDAEGDDMSREANEKKAV 120 Query: 121 KLQAQIPTVVAALARIRKGLEPVAPKEGLSLAENFLYMLWGKQPDIVSVKXXXXXXXXXX 180 KLQAQ+PT++AA+AR+R+G EPVAPK G S+AENFLYML G+ PD S+K Sbjct: 121 KLQAQLPTIIAAIARVRQGKEPVAPKAGASIAENFLYMLRGEDPDQTSIKALDQALVLHA 180 Query: 181 XXXXNASTFAGRVTVATLSDIYSGVTSAIGALKGPLHGGANEAVMKMLEEIGTLDAVEPY 240 NASTFA RVTVATLSDIYSGVTSAIGALKGPLHGGANEAVMKML E+G++D + Y Sbjct: 181 DHELNASTFAARVTVATLSDIYSGVTSAIGALKGPLHGGANEAVMKMLNEVGSIDRAQAY 240 Query: 241 IQGKLERREKIMGFGHRVYKNGDPRAKHLMKMSHELGLMKNDTALYDMSVKVEELITSKK 300 IQ KL+ REKIMGFGHRVYKNGDPRAKHL KMS ELG MK DT LYDMSV ++E +T++K Sbjct: 241 IQEKLDNREKIMGFGHRVYKNGDPRAKHLQKMSRELGEMKGDTTLYDMSVLIDETVTNQK 300 Query: 301 GLKPNVDFYSASVYTQLGIERELFTPIFAISRTSGWTAHILEQYEDNRIIRPRAEYTGMS 360 GLKPNVDFYSASVYTQLGIE ELFTPIFAISR SGWTAHILEQYE NRIIRPRAEYTGM+ Sbjct: 301 GLKPNVDFYSASVYTQLGIEHELFTPIFAISRVSGWTAHILEQYEGNRIIRPRAEYTGMT 360 Query: 361 EQKYVPVDER 370 +QKYVP++ R Sbjct: 361 DQKYVPIELR 370 >B1DDW7.1 B1DDW7_9BACL 2-methylcitrate synthase/citrate synthase II EC=2.3.3.1 Length = 370 Score = 496 bits (1276), Expect = e-138, Method: Compositional matrix adjust. Identities = 237/370 (64%), Positives = 288/370 (77%) Query: 1 MTATKGLEGIVAXXXXXXXXVDGVLTYRGYDIDDLAEHATFEETAYLLWFGNLPTTPELQ 60 MTATKGLEGIVA +DGVLTYRG +IDDLAE+ATFEE AYLLW+G LP EL Sbjct: 1 MTATKGLEGIVAASSSISSIIDGVLTYRGINIDDLAENATFEEVAYLLWYGKLPNRSELD 60 Query: 61 ALQRDLSAFAPIPEQVIAQMKLYPKEANTMXXXXXXXXXXXXYDEAADDMSREANEIKAV 120 LQ+ L ++ IP QVI Q+KLYP+ N+M YDE+A++MS EAN++KA+ Sbjct: 61 GLQQKLDQYSAIPAQVIEQIKLYPQGTNSMAALRTAVSSLALYDESANEMSPEANQLKAI 120 Query: 121 KLQAQIPTVVAALARIRKGLEPVAPKEGLSLAENFLYMLWGKQPDIVSVKXXXXXXXXXX 180 KLQAQ+PTVVAA ARIR+G EP+APK+G+S+A NFLYML G++P V++K Sbjct: 121 KLQAQLPTVVAAFARIREGKEPIAPKKGVSIAYNFLYMLTGEEPAEVAIKALDQALVLHA 180 Query: 181 XXXXNASTFAGRVTVATLSDIYSGVTSAIGALKGPLHGGANEAVMKMLEEIGTLDAVEPY 240 NASTFA RVTVATLSDIYSGVTSAIGALKGPLHGGANEAVM MLEEIG+++ VE Y Sbjct: 181 DHELNASTFAARVTVATLSDIYSGVTSAIGALKGPLHGGANEAVMVMLEEIGSMENVEGY 240 Query: 241 IQGKLERREKIMGFGHRVYKNGDPRAKHLMKMSHELGLMKNDTALYDMSVKVEELITSKK 300 I L ++KIMGFGHRVYK+GDPRAKHL +MS ELG + + LY+MS+K+E+++T +K Sbjct: 241 INNALANKQKIMGFGHRVYKDGDPRAKHLQQMSRELGKLTGNMELYEMSIKIEDMVTGQK 300 Query: 301 GLKPNVDFYSASVYTQLGIERELFTPIFAISRTSGWTAHILEQYEDNRIIRPRAEYTGMS 360 GLKPNVDFYSASVYT LGI R+LFTPIFAISR SGW+AHILEQYE+NR+IRPRA+Y G Sbjct: 301 GLKPNVDFYSASVYTTLGIPRDLFTPIFAISRVSGWSAHILEQYENNRLIRPRADYVGPV 360 Query: 361 EQKYVPVDER 370 KY+P+++R Sbjct: 361 NAKYIPIEQR 370 >Q5KWB5.1 Q5KWB5_GEOKA Citrate synthaseII EC=2.3.3.1 Length = 372 Score = 459 bits (1181), Expect = e-127, Method: Compositional matrix adjust. Identities = 223/371 (60%), Positives = 273/371 (73%), Gaps = 1/371 (0%) Query: 1 MTATKGLEGIVAXXXXXXXXVDGVLTYRGYDIDDLAEHATFEETAYLLWFGNLPTTPELQ 60 MT T+GLEG+VA +D LTY GYDIDDLAE+A+FEE YLLW LPT +L+ Sbjct: 1 MTVTRGLEGVVATTSSISSIIDDTLTYVGYDIDDLAENASFEEVVYLLWHRELPTKEQLE 60 Query: 61 ALQRDLSAFAPIPEQVIAQMKLYP-KEANTMXXXXXXXXXXXXYDEAADDMSREANEIKA 119 L++ L+ A IP ++I KLYP + + M YDE AD M++EAN KA Sbjct: 61 ELKKQLAENAEIPNEIIEHFKLYPIDKVHPMAALRTAVSLLGLYDEEADVMTKEANYRKA 120 Query: 120 VKLQAQIPTVVAALARIRKGLEPVAPKEGLSLAENFLYMLWGKQPDIVSVKXXXXXXXXX 179 ++LQA+IPT+V A AR+RKGLEPVAP++ LS A NFLYML GK+PD ++ + Sbjct: 121 IRLQAKIPTIVTAFARVRKGLEPVAPRKDLSFAANFLYMLTGKEPDDIATEAFNKALVLH 180 Query: 180 XXXXXNASTFAGRVTVATLSDIYSGVTSAIGALKGPLHGGANEAVMKMLEEIGTLDAVEP 239 NASTF RV VATLSDIYSG+T+AIGALKGPLHGGANEAVMKML EIGT+D VEP Sbjct: 181 ADHELNASTFTARVCVATLSDIYSGITAAIGALKGPLHGGANEAVMKMLTEIGTVDNVEP 240 Query: 240 YIQGKLERREKIMGFGHRVYKNGDPRAKHLMKMSHELGLMKNDTALYDMSVKVEELITSK 299 YI+ KL +EKIMGFGHRVY+ GDPRAKHL KMS +L + + Y+MS K+EE++TS+ Sbjct: 241 YIRRKLANKEKIMGFGHRVYRKGDPRAKHLKKMSEKLTKLVGEPHWYEMSTKIEEIVTSE 300 Query: 300 KGLKPNVDFYSASVYTQLGIERELFTPIFAISRTSGWTAHILEQYEDNRIIRPRAEYTGM 359 K L PNVDFYSASVY LGI+ +LFTPIFA+SRTSGW AHILEQY++NR+IRPRAEYTG Sbjct: 301 KALPPNVDFYSASVYHCLGIDHDLFTPIFAVSRTSGWLAHILEQYDNNRLIRPRAEYTGP 360 Query: 360 SEQKYVPVDER 370 ++ YVP+DER Sbjct: 361 GKRAYVPIDER 371 >C3IXS6.1 C3IXS6_9BACI 2-methylcitrate synthase/citrate synthase II EC=2.3.3.1 Length = 372 Score = 459 bits (1181), Expect = e-127, Method: Compositional matrix adjust. Identities = 223/371 (60%), Positives = 273/371 (73%), Gaps = 1/371 (0%) Query: 1 MTATKGLEGIVAXXXXXXXXVDGVLTYRGYDIDDLAEHATFEETAYLLWFGNLPTTPELQ 60 MT T+GLEG+VA +D LTY GYDIDDLAE+A+FEE YLLW LPT +L+ Sbjct: 1 MTVTRGLEGVVATTSSISSIIDDTLTYVGYDIDDLAENASFEEVVYLLWHRELPTKEQLE 60 Query: 61 ALQRDLSAFAPIPEQVIAQMKLYP-KEANTMXXXXXXXXXXXXYDEAADDMSREANEIKA 119 L++ L+ A IP ++I KLYP + + M YDE AD M++EAN KA Sbjct: 61 ELKKQLAENAEIPNEIIEHFKLYPIDKVHPMAALRTAVSLLGLYDEEADVMTKEANYRKA 120 Query: 120 VKLQAQIPTVVAALARIRKGLEPVAPKEGLSLAENFLYMLWGKQPDIVSVKXXXXXXXXX 179 ++LQA+IPT+V A AR+RKGLEPVAP++ LS A NFLYML GK+PD ++ + Sbjct: 121 IRLQAKIPTIVTAFARVRKGLEPVAPRKDLSFAANFLYMLTGKEPDDIATEAFNKALVLH 180 Query: 180 XXXXXNASTFAGRVTVATLSDIYSGVTSAIGALKGPLHGGANEAVMKMLEEIGTLDAVEP 239 NASTF RV VATLSDIYSG+T+AIGALKGPLHGGANEAVMKML EIGT+D VEP Sbjct: 181 ADHELNASTFTARVCVATLSDIYSGITAAIGALKGPLHGGANEAVMKMLTEIGTVDNVEP 240 Query: 240 YIQGKLERREKIMGFGHRVYKNGDPRAKHLMKMSHELGLMKNDTALYDMSVKVEELITSK 299 YI+ KL +EKIMGFGHRVY+ GDPRAKHL KMS +L + + Y+MS K+EE++TS+ Sbjct: 241 YIRRKLANKEKIMGFGHRVYRKGDPRAKHLKKMSEKLTKLVGEPHWYEMSTKIEEIVTSE 300 Query: 300 KGLKPNVDFYSASVYTQLGIERELFTPIFAISRTSGWTAHILEQYEDNRIIRPRAEYTGM 359 K L PNVDFYSASVY LGI+ +LFTPIFA+SRTSGW AHILEQY++NR+IRPRAEYTG Sbjct: 301 KALPPNVDFYSASVYHCLGIDHDLFTPIFAVSRTSGWLAHILEQYDNNRLIRPRAEYTGP 360 Query: 360 SEQKYVPVDER 370 ++ YVP+DER Sbjct: 361 GKRAYVPIDER 371 >B7YAF8.1 B7YAF8_9BACI 2-methylcitrate synthase/citrate synthase II EC=2.3.3.1 Length = 372 Score = 459 bits (1181), Expect = e-127, Method: Compositional matrix adjust. Identities = 223/371 (60%), Positives = 273/371 (73%), Gaps = 1/371 (0%) Query: 1 MTATKGLEGIVAXXXXXXXXVDGVLTYRGYDIDDLAEHATFEETAYLLWFGNLPTTPELQ 60 MT T+GLEG+VA +D LTY GYDIDDLAE+A+FEE YLLW LPT +L+ Sbjct: 1 MTVTRGLEGVVATTSSISSIIDDTLTYVGYDIDDLAENASFEEVVYLLWHRELPTKEQLE 60 Query: 61 ALQRDLSAFAPIPEQVIAQMKLYP-KEANTMXXXXXXXXXXXXYDEAADDMSREANEIKA 119 L++ L+ A IP ++I KLYP + + M YDE AD M++EAN KA Sbjct: 61 ELKKQLAENAEIPNEIIEHFKLYPIDKVHPMAALRTAVSLLGLYDEEADVMTKEANYRKA 120 Query: 120 VKLQAQIPTVVAALARIRKGLEPVAPKEGLSLAENFLYMLWGKQPDIVSVKXXXXXXXXX 179 ++LQA+IPT+V A AR+RKGLEPVAP++ LS A NFLYML GK+PD ++ + Sbjct: 121 IRLQAKIPTIVTAFARVRKGLEPVAPRKDLSFAANFLYMLTGKEPDDIATEAFNKALVLH 180 Query: 180 XXXXXNASTFAGRVTVATLSDIYSGVTSAIGALKGPLHGGANEAVMKMLEEIGTLDAVEP 239 NASTF RV VATLSDIYSG+T+AIGALKGPLHGGANEAVMKML EIGT+D VEP Sbjct: 181 ADHELNASTFTARVCVATLSDIYSGITAAIGALKGPLHGGANEAVMKMLTEIGTVDNVEP 240 Query: 240 YIQGKLERREKIMGFGHRVYKNGDPRAKHLMKMSHELGLMKNDTALYDMSVKVEELITSK 299 YI+ KL +EKIMGFGHRVY+ GDPRAKHL KMS +L + + Y+MS K+EE++TS+ Sbjct: 241 YIRRKLANKEKIMGFGHRVYRKGDPRAKHLKKMSEKLTKLVGEPHWYEMSTKIEEIVTSE 300 Query: 300 KGLKPNVDFYSASVYTQLGIERELFTPIFAISRTSGWTAHILEQYEDNRIIRPRAEYTGM 359 K L PNVDFYSASVY LGI+ +LFTPIFA+SRTSGW AHILEQY++NR+IRPRAEYTG Sbjct: 301 KALPPNVDFYSASVYHCLGIDHDLFTPIFAVSRTSGWLAHILEQYDNNRLIRPRAEYTGP 360 Query: 360 SEQKYVPVDER 370 ++ YVP+DER Sbjct: 361 GKRAYVPIDER 371 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.316 0.133 0.378 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,909,709,471 Number of extensions: 71582474 Number of successful extensions: 184060 Number of sequences better than 1.0e-05: 3656 Number of HSP's gapped: 174942 Number of HSP's successfully gapped: 3668 Length of query: 370 Length of database: 3,060,696,203 Length adjustment: 137 Effective length of query: 233 Effective length of database: 1,770,017,148 Effective search space: 412413995484 Effective search space used: 412413995484 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 132 (55.5 bits)