BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0333.0002.p (313 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KBP7.1 B3KBP7_9BACI Malate dehydrogenase, NAD-dependent 536 e-150 B1DDW5.1 B1DDW5_9BACL Malate dehydrogenase, NAD-dependent 505 e-141 C0Z7X5.1 C0Z7X5_BREBN Malate dehydrogenase EC=1.1.1.37 467 e-130 Q2B2I2.1 Q2B2I2_9BACI Malate dehydrogenase EC=1.1.1.37 453 e-125 C3B931.1 C3B931_BACMY Malate dehydrogenase 449 e-124 >B3KBP7.1 B3KBP7_9BACI Malate dehydrogenase, NAD-dependent Length = 313 Score = 536 bits (1380), Expect = e-150, Method: Compositional matrix adjust. Identities = 265/313 (84%), Positives = 283/313 (90%) Query: 1 VAIKRNKITVVGAGFTGSTTALMLAQKELGDVVLLDIPQLENPTKGKVLDMLEAGPVLKF 60 +AI R KITVVGAGFTG+TTALMLAQKELGDVVLLDIPQLENPTKGK LDMLEA PV F Sbjct: 1 MAITRKKITVVGAGFTGATTALMLAQKELGDVVLLDIPQLENPTKGKALDMLEASPVQGF 60 Query: 61 DARITGTSSYEDAADSDIVIITAGIARKPGMSRDDLVNTNAGIVKSVCENVKRVAPESIV 120 D+ I GTS+YEDAADSDIVIITAG+ARKPGMSRDDLVNTNAGIVKSVCENVK AP S V Sbjct: 61 DSNIVGTSNYEDAADSDIVIITAGVARKPGMSRDDLVNTNAGIVKSVCENVKTHAPNSTV 120 Query: 121 IILSNPVDAMTYVAFKTLGFPKNRVIGQSGVLDTARYCTFIAEELNVSVEDVRGFVLGGH 180 IILSNPVDAMTYVA+ TLGFPKNRVIGQSGVLDTARYCTFIA+ELNVSVEDVRGFVLGGH Sbjct: 121 IILSNPVDAMTYVAYNTLGFPKNRVIGQSGVLDTARYCTFIAQELNVSVEDVRGFVLGGH 180 Query: 181 GDDMVPLVRYSSVGGIPIETLIPAERIAEIVQRTRVGGGEIVSLLGNGSAYYAPAASLVQ 240 GDDMVPLVRYSSVGGIPI+TLIPAERI IVQRTRVGGGEIV+LLGNGSAYYAPAASLVQ Sbjct: 181 GDDMVPLVRYSSVGGIPIDTLIPAERIEAIVQRTRVGGGEIVNLLGNGSAYYAPAASLVQ 240 Query: 241 MTEAVLKDKKRILPVIALLEGEYGYDGLFMGIPALLGAEGIEKIFXXXXXXXXXXXXDKS 300 MTEA+LKDKKRI+PVIALLEGEYGYD LFMG+PA+LG +GIE+IF DKS Sbjct: 241 MTEAILKDKKRIIPVIALLEGEYGYDNLFMGVPAILGGDGIERIFELELTAEEKAALDKS 300 Query: 301 ADSVRSVTSVVTI 313 A+SVR+VTSVV + Sbjct: 301 AESVRNVTSVVNL 313 >B1DDW5.1 B1DDW5_9BACL Malate dehydrogenase, NAD-dependent Length = 313 Score = 505 bits (1300), Expect = e-141, Method: Compositional matrix adjust. Identities = 246/312 (78%), Positives = 277/312 (88%) Query: 1 VAIKRNKITVVGAGFTGSTTALMLAQKELGDVVLLDIPQLENPTKGKVLDMLEAGPVLKF 60 +AIKR KITVVGAGFTG+TTALM AQKELGD+VL+DIPQLENPTKGK LDMLE+ PV Sbjct: 1 MAIKRRKITVVGAGFTGATTALMAAQKELGDIVLVDIPQLENPTKGKALDMLESTPVQGL 60 Query: 61 DARITGTSSYEDAADSDIVIITAGIARKPGMSRDDLVNTNAGIVKSVCENVKRVAPESIV 120 DA ITGTS+Y++ DSD+VIITAGIARKPGMSRDDLVNTNAGIV+SVCE+VK +P + V Sbjct: 61 DANITGTSNYDETKDSDVVIITAGIARKPGMSRDDLVNTNAGIVRSVCESVKATSPNAYV 120 Query: 121 IILSNPVDAMTYVAFKTLGFPKNRVIGQSGVLDTARYCTFIAEELNVSVEDVRGFVLGGH 180 IILSNPVDAMTYVA++TLGFPKNRVIGQSGVLDTARYCTFIA+ELNVSVEDVRGFVLGGH Sbjct: 121 IILSNPVDAMTYVAYQTLGFPKNRVIGQSGVLDTARYCTFIAQELNVSVEDVRGFVLGGH 180 Query: 181 GDDMVPLVRYSSVGGIPIETLIPAERIAEIVQRTRVGGGEIVSLLGNGSAYYAPAASLVQ 240 GDDMVPLVRYS+VGGIPIE LIP++RI IVQR+RVGGGEIV+LLGNGSAYYAPAASLVQ Sbjct: 181 GDDMVPLVRYSNVGGIPIEKLIPSDRIEAIVQRSRVGGGEIVNLLGNGSAYYAPAASLVQ 240 Query: 241 MTEAVLKDKKRILPVIALLEGEYGYDGLFMGIPALLGAEGIEKIFXXXXXXXXXXXXDKS 300 MTEA+LKDKKRILPVIALLEGEYGY+ LFMG+P ++G +GIEK+ DKS Sbjct: 241 MTEAILKDKKRILPVIALLEGEYGYNNLFMGVPTIIGGDGIEKVIELELTSEEKAALDKS 300 Query: 301 ADSVRSVTSVVT 312 A+SV++V VVT Sbjct: 301 AESVQNVIKVVT 312 >C0Z7X5.1 C0Z7X5_BREBN Malate dehydrogenase EC=1.1.1.37 Length = 312 Score = 467 bits (1202), Expect = e-130, Method: Compositional matrix adjust. Identities = 228/311 (73%), Positives = 260/311 (83%) Query: 1 VAIKRNKITVVGAGFTGSTTALMLAQKELGDVVLLDIPQLENPTKGKVLDMLEAGPVLKF 60 +A R KI V+G+GFTG+TTA +L QKELGD+VL+DIPQLENPTKGK LDM+E+ PVL F Sbjct: 1 MAFNRKKIAVIGSGFTGATTAFILGQKELGDIVLVDIPQLENPTKGKALDMMESAPVLGF 60 Query: 61 DARITGTSSYEDAADSDIVIITAGIARKPGMSRDDLVNTNAGIVKSVCENVKRVAPESIV 120 DA ITGT++YED D+D+VIITAGIARKPGMSRDDLVNTNAGI+KSV E VK AP SIV Sbjct: 61 DASITGTANYEDIKDADLVIITAGIARKPGMSRDDLVNTNAGIMKSVAEQVKTYAPNSIV 120 Query: 121 IILSNPVDAMTYVAFKTLGFPKNRVIGQSGVLDTARYCTFIAEELNVSVEDVRGFVLGGH 180 ++LSNPVDAMTY FKT GFPK RVIGQSGVLDTAR+ TF+A ELNVSVEDV GFVLGGH Sbjct: 121 LVLSNPVDAMTYTFFKTSGFPKERVIGQSGVLDTARFRTFVAMELNVSVEDVTGFVLGGH 180 Query: 181 GDDMVPLVRYSSVGGIPIETLIPAERIAEIVQRTRVGGGEIVSLLGNGSAYYAPAASLVQ 240 GDDMVPLVRYS GGIP+E LIP +R+ IV+RTR GGGEIV+LLGNGSAYYAPAASLVQ Sbjct: 181 GDDMVPLVRYSYAGGIPLEKLIPKDRLDAIVERTRKGGGEIVNLLGNGSAYYAPAASLVQ 240 Query: 241 MTEAVLKDKKRILPVIALLEGEYGYDGLFMGIPALLGAEGIEKIFXXXXXXXXXXXXDKS 300 M EA+LKDKKRILP IALL+GEYGY+ L++G+P LLG GIEK+ DKS Sbjct: 241 MAEAILKDKKRILPSIALLQGEYGYNDLYLGVPTLLGTNGIEKVIELDLTAEEKAALDKS 300 Query: 301 ADSVRSVTSVV 311 ADSVRSV +V+ Sbjct: 301 ADSVRSVMAVL 311 >Q2B2I2.1 Q2B2I2_9BACI Malate dehydrogenase EC=1.1.1.37 Length = 312 Score = 453 bits (1165), Expect = e-125, Method: Compositional matrix adjust. Identities = 215/311 (69%), Positives = 259/311 (83%) Query: 1 VAIKRNKITVVGAGFTGSTTALMLAQKELGDVVLLDIPQLENPTKGKVLDMLEAGPVLKF 60 + +KR KI+V+G GFTG+TTA +LAQKELGDVVL+DIPQ+ENPTKGK LDMLEA PV F Sbjct: 1 MTLKRKKISVIGGGFTGATTAFLLAQKELGDVVLVDIPQMENPTKGKALDMLEASPVQGF 60 Query: 61 DARITGTSSYEDAADSDIVIITAGIARKPGMSRDDLVNTNAGIVKSVCENVKRVAPESIV 120 DA ITGTSSYED DSDIV+ITAGIARKPGMSRDDLV TN I+KSV + + + +PES + Sbjct: 61 DANITGTSSYEDTKDSDIVVITAGIARKPGMSRDDLVQTNQKIMKSVAQEIVKYSPESYI 120 Query: 121 IILSNPVDAMTYVAFKTLGFPKNRVIGQSGVLDTARYCTFIAEELNVSVEDVRGFVLGGH 180 I+L+NPVDAMTY F GFPKNRVIGQSG+LDTAR+ TF+A+ELN+SV+D+ GFVLGGH Sbjct: 121 IVLTNPVDAMTYTVFTESGFPKNRVIGQSGILDTARFRTFVAQELNLSVKDITGFVLGGH 180 Query: 181 GDDMVPLVRYSSVGGIPIETLIPAERIAEIVQRTRVGGGEIVSLLGNGSAYYAPAASLVQ 240 GDDMVPLVRYS GGIP+ETLIP +R+ IV+RTR GGGEIV+LLGNGSAYYAPAASLV+ Sbjct: 181 GDDMVPLVRYSYAGGIPLETLIPKDRLDAIVERTRKGGGEIVNLLGNGSAYYAPAASLVE 240 Query: 241 MTEAVLKDKKRILPVIALLEGEYGYDGLFMGIPALLGAEGIEKIFXXXXXXXXXXXXDKS 300 M EA+LKD++R+LP IA LEGEYGY+G+++G+P +LGA GIEKI DKS Sbjct: 241 MCEAILKDQRRVLPSIAYLEGEYGYEGIYLGVPTILGANGIEKIIELELTDEEKAALDKS 300 Query: 301 ADSVRSVTSVV 311 A++VR+V V+ Sbjct: 301 AEAVRNVMDVL 311 >C3B931.1 C3B931_BACMY Malate dehydrogenase Length = 312 Score = 449 bits (1155), Expect = e-124, Method: Compositional matrix adjust. Identities = 215/311 (69%), Positives = 258/311 (82%) Query: 1 VAIKRNKITVVGAGFTGSTTALMLAQKELGDVVLLDIPQLENPTKGKVLDMLEAGPVLKF 60 + IKR K++V+GAGFTG+TTA +LAQKEL DVVL+DIPQLENPTKGK LDMLEA PV F Sbjct: 1 MTIKRKKVSVIGAGFTGATTAFLLAQKELADVVLVDIPQLENPTKGKALDMLEASPVQGF 60 Query: 61 DARITGTSSYEDAADSDIVIITAGIARKPGMSRDDLVNTNAGIVKSVCENVKRVAPESIV 120 DA I GTS Y D ADSD+V+ITAGIARKPGMSRDDLV TN+ I+KS+ +++ + +P +I+ Sbjct: 61 DANIIGTSDYVDTADSDVVVITAGIARKPGMSRDDLVATNSKIMKSITKDIAKHSPNAII 120 Query: 121 IILSNPVDAMTYVAFKTLGFPKNRVIGQSGVLDTARYCTFIAEELNVSVEDVRGFVLGGH 180 ++L+NPVDAMTY FK GFPK RVIGQSGVLDTAR+ TFIA+ELN+SV+D+ GFVLGGH Sbjct: 121 VVLTNPVDAMTYSVFKEAGFPKERVIGQSGVLDTARFRTFIAQELNLSVKDITGFVLGGH 180 Query: 181 GDDMVPLVRYSSVGGIPIETLIPAERIAEIVQRTRVGGGEIVSLLGNGSAYYAPAASLVQ 240 GDDMVPLVRYS GGIP+ETLIPAER+ EIV+RTR GGGEIVSLLGNGSAYYAPAASLV+ Sbjct: 181 GDDMVPLVRYSYAGGIPLETLIPAERLEEIVERTRKGGGEIVSLLGNGSAYYAPAASLVE 240 Query: 241 MTEAVLKDKKRILPVIALLEGEYGYDGLFMGIPALLGAEGIEKIFXXXXXXXXXXXXDKS 300 MTEA+LKD++R+LP IA LEGEYGY L++G+P +LG GIEKI D+S Sbjct: 241 MTEAILKDQRRVLPAIAYLEGEYGYSDLYLGVPVILGGNGIEKIIELELREEEKEALDRS 300 Query: 301 ADSVRSVTSVV 311 +SVR+V V+ Sbjct: 301 VESVRNVMKVL 311 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.318 0.137 0.379 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,715,230,945 Number of extensions: 71753969 Number of successful extensions: 218460 Number of sequences better than 1.0e-05: 4067 Number of HSP's gapped: 213549 Number of HSP's successfully gapped: 4068 Length of query: 313 Length of database: 3,060,696,203 Length adjustment: 135 Effective length of query: 178 Effective length of database: 1,788,859,178 Effective search space: 318416933684 Effective search space used: 318416933684 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)