BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0331.0003.p (246 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1DG99.1 B1DG99_9BACL Short-chain dehydrogenase/reductase SDR 407 e-112 A3WK52.1 A3WK52_9GAMM Putative uncharacterized protein 278 3e-73 Q2KDH6.1 Q2KDH6_RHIEC Putative oxidoreductase protein 271 4e-71 B1YGL2.1 B1YGL2_EXIS2 Short-chain dehydrogenase/reductase SDR 269 2e-70 B1JA95.1 B1JA95_PSEPW Short-chain dehydrogenase/reductase SDR 266 1e-69 >B1DG99.1 B1DG99_9BACL Short-chain dehydrogenase/reductase SDR Length = 246 Score = 407 bits (1046), Expect = e-112, Method: Compositional matrix adjust. Identities = 197/245 (80%), Positives = 227/245 (92%) Query: 1 MENIQNKVVIITGASSGIGEATAKLLAQNGAKVVLAARREERLNAIVNEIKQGGGEAVSV 60 MENI++KVVIITGASSGIGEATAKLLA GAKVVLAARRE+RL ++VN+IKQ GGEAVSV Sbjct: 1 MENIKDKVVIITGASSGIGEATAKLLADQGAKVVLAARREDRLQSVVNDIKQNGGEAVSV 60 Query: 61 KADVISAEDMQKLAKFALNTYGRIDVWVNNAGVMPSSRLHELRVGEWDQMIDVNIKGVLY 120 +ADV+S+EDM++LA+FAL+ YGRIDV VNNAG+MPSSR++ELRV EW+QMIDVNIKGVLY Sbjct: 61 RADVVSSEDMKRLAQFALDKYGRIDVLVNNAGIMPSSRMNELRVEEWEQMIDVNIKGVLY 120 Query: 121 GIAAVLPAMREQQSGHIINLSSTAGYQVSPTNAVYAATKFAVRAISEGLRLEESSASRIR 180 GIAAVLP MREQ+SGH+INLSSTAGY VS T+AVYAATKFAVRAISEGLRLEES+ S IR Sbjct: 121 GIAAVLPVMREQKSGHVINLSSTAGYHVSATSAVYAATKFAVRAISEGLRLEESADSGIR 180 Query: 181 STLIAPGLTKTELFDSITSPEVQTIANQLSGVGISPYSIARAIAFAINEPDDTLISEIMV 240 ST+++PGLT TELF SITSPEVQ +A+Q+ G+GI+P SIARAIAFAINEPDD L+SEIMV Sbjct: 181 STVVSPGLTNTELFSSITSPEVQAMASQIGGMGINPSSIARAIAFAINEPDDALVSEIMV 240 Query: 241 RPTAL 245 RPTAL Sbjct: 241 RPTAL 245 >A3WK52.1 A3WK52_9GAMM Putative uncharacterized protein Length = 245 Score = 278 bits (711), Expect = 3e-73, Method: Compositional matrix adjust. Identities = 142/243 (58%), Positives = 176/243 (72%), Gaps = 2/243 (0%) Query: 1 MENIQNKVVIITGASSGIGEATAKLLAQNGAKVVLAARREERLNAIVNEIKQGGGEAVSV 60 M + NKVVIITGASSG+GE TAK+LA GAK+VL ARREERL A+ + IK GGEA+ Sbjct: 1 MSEVANKVVIITGASSGLGEETAKMLASKGAKLVLGARREERLKALADSIKNDGGEAIFK 60 Query: 61 KADVISAEDMQKLAKFALNTYGRIDVWVNNAGVMPSSRLHELRVGEWDQMIDVNIKGVLY 120 DV +Q LA AL +GRIDV VNNAG+MP + L EL++ EWDQMIDVNIKGV+Y Sbjct: 61 TVDVTDKSQVQALADAALEQFGRIDVLVNNAGLMPLAPLDELKIDEWDQMIDVNIKGVMY 120 Query: 121 GIAAVLPAMREQQSGHIINLSSTAGYQVSPTNAVYAATKFAVRAISEGLRLEESSASRIR 180 G+AAVLP+MR+Q+SGHIINLSS AG+ V P VY ATKFAV+AISEG+R E S IR Sbjct: 121 GVAAVLPSMRKQKSGHIINLSSVAGHVVFPGATVYCATKFAVKAISEGIRQE--SNGEIR 178 Query: 181 STLIAPGLTKTELFDSITSPEVQTIANQLSGVGISPYSIARAIAFAINEPDDTLISEIMV 240 +T I+PG TEL D I+ + + +A+ L I SIARAI FAI +P D ++E+++ Sbjct: 179 ATNISPGAVSTELTDHISHKDSKEMADDLYQDAIGSDSIARAITFAIEQPGDVDVNEMII 238 Query: 241 RPT 243 RPT Sbjct: 239 RPT 241 >Q2KDH6.1 Q2KDH6_RHIEC Putative oxidoreductase protein Length = 244 Score = 271 bits (692), Expect = 4e-71, Method: Compositional matrix adjust. Identities = 140/246 (56%), Positives = 176/246 (71%), Gaps = 3/246 (1%) Query: 1 MENIQNKVVIITGASSGIGEATAKLLAQNGAKVVLAARREERLNAIVNEIKQGGGEAVSV 60 M +I+ KV+ ITGASSGIGEATAK+LA GA++V+ ARR ERL A+ EI+ GGG Sbjct: 1 MSDIEGKVIAITGASSGIGEATAKVLAAAGAQIVIGARRSERLEALAGEIEAGGGTVRLR 60 Query: 61 KADVISAEDMQKLAKFALNTYGRIDVWVNNAGVMPSSRLHELRVGEWDQMIDVNIKGVLY 120 K DV ++ A FA + +GR+DV VNNAGVMP S L L+V EWD+M+DVNIKGVLY Sbjct: 61 KLDVTDRAQVEAFAGFARSEFGRLDVIVNNAGVMPLSPLDALKVEEWDRMVDVNIKGVLY 120 Query: 121 GIAAVLPAMREQQSGHIINLSSTAGYQVSPTNAVYAATKFAVRAISEGLRLEESSASRIR 180 GIAA LP M+ Q SG IINLSS G+ VSPT AVY ATK+AVRAIS+GLR E +RIR Sbjct: 121 GIAAALPIMKAQGSGQIINLSSIGGHSVSPTAAVYCATKYAVRAISDGLRQE---TNRIR 177 Query: 181 STLIAPGLTKTELFDSITSPEVQTIANQLSGVGISPYSIARAIAFAINEPDDTLISEIMV 240 T+I+PG T +EL ++IT P + + ISP ++A AI +AI++PDD +SEI+V Sbjct: 178 VTVISPGTTTSELAETITDPTARDAMKAFRAITISPEAVANAILYAISQPDDVDVSEIIV 237 Query: 241 RPTALP 246 RPTA P Sbjct: 238 RPTASP 243 >B1YGL2.1 B1YGL2_EXIS2 Short-chain dehydrogenase/reductase SDR Length = 242 Score = 269 bits (687), Expect = 2e-70, Method: Compositional matrix adjust. Identities = 143/241 (59%), Positives = 186/241 (77%), Gaps = 2/241 (0%) Query: 4 IQNKVVIITGASSGIGEATAKLLAQNGAKVVLAARREERLNAIVNEIKQGGGEAVSVKAD 63 I+NKVVIITGASSGIGEATAKLLA+ GA++VLAARRE+RL + EI+Q GG+AV D Sbjct: 2 IENKVVIITGASSGIGEATAKLLAKQGAQLVLAARREDRLKTLQQEIEQLGGKAVYQVTD 61 Query: 64 VISAEDMQKLAKFALNTYGRIDVWVNNAGVMPSSRLHELRVGEWDQMIDVNIKGVLYGIA 123 V + +++LAK A +T+G +DV VNNAG+MP S+L++ + EW+ M+DVNIKGVLYGI Sbjct: 62 VTDSAQVEQLAKLAQDTFGSVDVLVNNAGLMPLSKLNKNKQDEWNTMVDVNIKGVLYGIG 121 Query: 124 AVLPAMREQQSGHIINLSSTAGYQVSPTNAVYAATKFAVRAISEGLRLEESSASRIRSTL 183 AVLP MREQ+ GHIIN+SS AG+ V P++AVY+ TKFAVRAI+EGLR EES + IR+T+ Sbjct: 122 AVLPYMREQKRGHIINISSVAGHDVMPSSAVYSGTKFAVRAITEGLRKEESVENNIRATI 181 Query: 184 IAPGLTKTELFDSITSPEVQTIANQLSGVGISPYSIARAIAFAINEPDDTLISEIMVRPT 243 I+PG TEL D IT E++ L + +IARAIA+A+NEPDD ++EI++RPT Sbjct: 182 ISPGAVDTELKDHITDEEIKQGIGDLK--AMDADAIARAIAYAVNEPDDVAVNEILIRPT 239 Query: 244 A 244 A Sbjct: 240 A 240 >B1JA95.1 B1JA95_PSEPW Short-chain dehydrogenase/reductase SDR Length = 245 Score = 266 bits (680), Expect = 1e-69, Method: Compositional matrix adjust. Identities = 135/246 (54%), Positives = 177/246 (71%), Gaps = 2/246 (0%) Query: 1 MENIQNKVVIITGASSGIGEATAKLLAQNGAKVVLAARREERLNAIVNEIKQGGGEAVSV 60 M I+ KV++ITGASSGIGEATA+LLA GA+VVL ARR +RL + EI+ G A + Sbjct: 1 MHGIEQKVIVITGASSGIGEATARLLASKGARVVLGARRTDRLETLAREIRSAGDVADVL 60 Query: 61 KADVISAEDMQKLAKFALNTYGRIDVWVNNAGVMPSSRLHELRVGEWDQMIDVNIKGVLY 120 DV + +DMQ FA+ +GR+DV +NNAGVMP S+L L+V EW++MIDVNI+GVL+ Sbjct: 61 ALDVTNLDDMQSFIDFAIELHGRVDVLINNAGVMPLSKLEALKVDEWNRMIDVNIRGVLH 120 Query: 121 GIAAVLPAMREQQSGHIINLSSTAGYQVSPTNAVYAATKFAVRAISEGLRLEESSASRIR 180 GIAA LP M+EQ++G IIN++S Y VSPT AVY ATK+AVRAISEGLR E IR Sbjct: 121 GIAATLPLMQEQRAGQIINIASIGAYAVSPTAAVYCATKYAVRAISEGLRQE--VGGDIR 178 Query: 181 STLIAPGLTKTELFDSITSPEVQTIANQLSGVGISPYSIARAIAFAINEPDDTLISEIMV 240 T+IAPG+T++EL DSI+ +T + + I +IARAIA+A+ +P D +SE++V Sbjct: 179 VTVIAPGVTESELADSISDEGGRTEMREFRKIAIPASAIARAIAYAVEQPADVDVSELIV 238 Query: 241 RPTALP 246 RPTA P Sbjct: 239 RPTASP 244 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.314 0.130 0.350 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,234,177,987 Number of extensions: 44482533 Number of successful extensions: 208286 Number of sequences better than 1.0e-05: 32184 Number of HSP's gapped: 174862 Number of HSP's successfully gapped: 32266 Length of query: 246 Length of database: 3,060,696,203 Length adjustment: 132 Effective length of query: 114 Effective length of database: 1,817,122,223 Effective search space: 207151933422 Effective search space used: 207151933422 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 129 (54.3 bits)