BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0331.0002.p (196 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1DG98.1 B1DG98_9BACL Transcriptional regulator, TetR family 227 4e-58 A6LXA3.1 A6LXA3_CLOB8 Transcriptional regulator, TetR family 164 5e-39 B1DGV0.1 B1DGV0_9BACL Transcriptional regulator, TetR family 148 3e-34 B3KEK7.1 B3KEK7_9BACI Regulatory protein TetR 136 1e-30 C0ZEV6.1 C0ZEV6_BREBN Probable transcriptional regulator 135 1e-30 >B1DG98.1 B1DG98_9BACL Transcriptional regulator, TetR family Length = 165 Score = 227 bits (579), Expect = 4e-58, Method: Compositional matrix adjust. Identities = 108/163 (66%), Positives = 131/163 (80%), Gaps = 6/163 (3%) Query: 27 SEKDLEQITINEIAERANVNRGTVYLHYSDKYDLLDKCIEDHINELISLCKKQEANPVSQ 86 +EK+ EQITINEIA+RANVNRGTVYLHYSDKYDLLDKC E+HI E+IS+CK +E+ + Sbjct: 5 TEKEFEQITINEIADRANVNRGTVYLHYSDKYDLLDKCTEEHIGEIISICKNRES---GE 61 Query: 87 GMVYNSKPVFDYIRDHFPFFSAMFSNQRASLFRDRLLHFISASLMDKMEGPDNGHVIDNE 146 G N + VFDY ++HFPFFSAMFSN+R LFRD+LLHF S ++M KME + ID E Sbjct: 62 G---NPQQVFDYFQEHFPFFSAMFSNERVFLFRDQLLHFFSVNMMLKMEQTNEKPDIDQE 118 Query: 147 LNAQFLASAFIGIVEWWIRHQMPHSTDFMADQVRKLFKKNQIY 189 + AQF+ASAFIG+VEWWIR MPHST FMADQV+ LF+ NQ+Y Sbjct: 119 IKAQFMASAFIGVVEWWIRRGMPHSTPFMADQVQHLFENNQVY 161 >A6LXA3.1 A6LXA3_CLOB8 Transcriptional regulator, TetR family Length = 185 Score = 164 bits (414), Expect = 5e-39, Method: Compositional matrix adjust. Identities = 79/183 (43%), Positives = 121/183 (66%), Gaps = 5/183 (2%) Query: 8 DRRIIRTKQSITKSFLELFSEKDLEQITINEIAERANVNRGTVYLHYSDKYDLLDKCIED 67 DRRI ++KQ+I +F++L SEKD E+ITINEIAE ANVNRGTVYLHY DK+DL++KCI+ Sbjct: 2 DRRIEKSKQAIMGAFIKLMSEKDFEKITINEIAEEANVNRGTVYLHYEDKFDLMNKCIDT 61 Query: 68 HINELISLC--KKQEANPVSQGMVYNSKPVFDYIRDHFPFFSAMFSNQRASLFRDRLLHF 125 H+N+L C + +N S+ + + F Y+ H F+S M +N+ FR+R+L Sbjct: 62 HLNQLCDSCISDGESSNFDSKASLLQT---FQYLEKHAIFYSNMLTNKAMPAFRERILTM 118 Query: 126 ISASLMDKMEGPDNGHVIDNELNAQFLASAFIGIVEWWIRHQMPHSTDFMADQVRKLFKK 185 S+ + ++ + I + AQ++ASA +G++EWWI + MP+ +MA+Q+ +L K+ Sbjct: 119 ALKSMEEHLDMTGSNENISKNIMAQYVASAAVGVMEWWIVNSMPYPAAYMAEQLWQLLKR 178 Query: 186 NQI 188 QI Sbjct: 179 EQI 181 >B1DGV0.1 B1DGV0_9BACL Transcriptional regulator, TetR family Length = 185 Score = 148 bits (373), Expect = 3e-34, Method: Compositional matrix adjust. Identities = 78/192 (40%), Positives = 119/192 (61%), Gaps = 17/192 (8%) Query: 4 ITKTDRRIIRTKQSITKSFLELFSEKDLEQITINEIAERANVNRGTVYLHYSDKYDLLDK 63 + K DRRI++T++S+ + ++L SEK ++ITI +IA+RAN+NRGT+YLHY DKYDLLDK Sbjct: 1 MPKVDRRIVKTQESLKHAVIDLMSEKSFDEITIQDIADRANLNRGTIYLHYQDKYDLLDK 60 Query: 64 CIEDHINELISLCKKQEANPVSQGMVYNSKPVFDYIRDHFPFFSAMFSNQRA----SLFR 119 IE H+NEL + K +V P F Y ++F FFS M +++ A S FR Sbjct: 61 LIESHLNELGEMDKWACEMDWIDALV----PYFKYFEENFLFFSTMLASKEAKGAPSSFR 116 Query: 120 DRLLHFISASLMDKMEG-----PDNGHVIDNELNAQFLASAFIGIVEWWIRHQMPHSTDF 174 RLL+ S M+ +G + + +E+ Q+ +A++G++EWWIR+ MPH + Sbjct: 117 TRLLN----SFMEGFKGEIDRESERNTDLSDEVMLQYAGTAYVGVIEWWIRNGMPHPPEV 172 Query: 175 MADQVRKLFKKN 186 MA QV L +++ Sbjct: 173 MAKQVGVLLERS 184 >B3KEK7.1 B3KEK7_9BACI Regulatory protein TetR Length = 182 Score = 136 bits (342), Expect = 1e-30, Method: Compositional matrix adjust. Identities = 71/193 (36%), Positives = 118/193 (61%), Gaps = 22/193 (11%) Query: 4 ITKTDRRIIRTKQSITKSFLELFSEKDLEQITINEIAERANVNRGTVYLHYSDKYDLLDK 63 + K DRRI++T++++ K+ +EL ++K+ + ITI +IA++AN+NRGT+YLHY DKYDLL++ Sbjct: 1 MAKVDRRILKTQEALRKAVIELMADKNFDDITIQDIADQANLNRGTIYLHYQDKYDLLNQ 60 Query: 64 CIEDHINELISL----CKKQEANPVSQGMVYNSKPVFDYIRDHFPFFSAMFSNQRA-SLF 118 +E HI EL + C+ + A+ + P F+Y ++ FS M +++ A S F Sbjct: 61 IMEAHIQELKEMDTWACEMEWADALV--------PYFEYFEKNYVLFSTMLASKGAPSSF 112 Query: 119 RDRLLHFISASLMDKMEGPDNGHVIDN-----ELNAQFLASAFIGIVEWWIRHQMPHSTD 173 R RLL S M+ +G + N ++ Q+ +A++G++EWWIR+ MP+S Sbjct: 113 RTRLL----TSFMEGFKGEIDKESGKNAELHEDVMLQYAGTAYVGVIEWWIRNGMPYSPQ 168 Query: 174 FMADQVRKLFKKN 186 MA QV L ++ Sbjct: 169 VMAKQVGTLLDRS 181 >C0ZEV6.1 C0ZEV6_BREBN Probable transcriptional regulator Length = 191 Score = 135 bits (341), Expect = 1e-30, Method: Compositional matrix adjust. Identities = 67/181 (37%), Positives = 107/181 (59%), Gaps = 7/181 (3%) Query: 9 RRIIRTKQSITKSFLELFSEKDLEQITINEIAERANVNRGTVYLHYSDKYDLLDKCIEDH 68 RRI +++Q+I +F+ L SEK+ E ITIN+IA ANVNRGTVYLH+ DKYDL ++CI Sbjct: 3 RRIQKSRQAIIDAFMRLMSEKEFESITINQIAVEANVNRGTVYLHFDDKYDLREQCIAAQ 62 Query: 69 INELISLCKKQE---ANPVSQGMVYNSKPVFDYIRDHFPFFSAMFSNQRASLFRDRLLHF 125 IN+L+ C + P +++ F+Y+ H F+S M ++ +++FR+++ Sbjct: 63 INQLLRHCMSGDHLVHLPSKTALLHT----FEYLEQHASFYSIMLKSKGSTVFRNQMETM 118 Query: 126 ISASLMDKMEGPDNGHVIDNELNAQFLASAFIGIVEWWIRHQMPHSTDFMADQVRKLFKK 185 SL + ++ + +D ++ QFL SA +GI+EWWI MP+ M +Q L + Sbjct: 119 FGESLSEYLDSINLDKEMDRDITVQFLTSATVGILEWWIIRSMPYPPSVMVEQFWNLLNR 178 Query: 186 N 186 Sbjct: 179 G 179 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.322 0.136 0.399 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,084,391,091 Number of extensions: 41536875 Number of successful extensions: 121278 Number of sequences better than 1.0e-05: 838 Number of HSP's gapped: 120952 Number of HSP's successfully gapped: 839 Length of query: 196 Length of database: 3,060,696,203 Length adjustment: 129 Effective length of query: 67 Effective length of database: 1,845,385,268 Effective search space: 123640812956 Effective search space used: 123640812956 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 127 (53.5 bits)