BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0327.0022.p (390 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K7H2.1 B3K7H2_9BACI Sensor DegS domain protein 640 0.0 B1DEC1.1 B1DEC1_9BACL Histidine kinase 505 e-141 C0Z731.1 C0Z731_BREBN Two-component sensor histidine kinase EC=2... 355 3e-96 A5D2A9.1 A5D2A9_PELTS Sensor protein DegS and signal transductio... 332 5e-89 B7GL15.1 B7GL15_ANOFW Signal transduction histidine kinase 325 3e-87 >B3K7H2.1 B3K7H2_9BACI Sensor DegS domain protein Length = 384 Score = 640 bits (1651), Expect = 0.0, Method: Compositional matrix adjust. Identities = 316/385 (82%), Positives = 355/385 (92%), Gaps = 1/385 (0%) Query: 6 VEFQADAIDRVIKNTIDVMESSKYQIFEILQVARDELVALTKELQRVMEETDETLQKVDK 65 +++QADAIDRVIKN I VME SKYQ+FEIL+ +R EL +L ELQR ++ET ETL+KVD+ Sbjct: 1 MDYQADAIDRVIKNAIKVMEDSKYQMFEILEASRQELASLNHELQRTLKETTETLEKVDQ 60 Query: 66 LELQYHRSRIRLTEVSRDFVRYSEKDIRVAYEKATELQLELMMTREREVYLRSRRDELQM 125 LEL Y RSRIRLTEVSRDFVRY E+DI+ AYEKAT+LQL+LM+ RE+E+YL++RRDELQ Sbjct: 61 LELNYRRSRIRLTEVSRDFVRYKEEDIKQAYEKATQLQLDLMIYREKEMYLKARRDELQK 120 Query: 126 RVRSVENSVERAESIGSQMSVVLEYLSGELGQVTRIVESAKNRQMIGLKIILAQEEERKR 185 RVR+VENSVERAESIGSQM VVLEYLSGELGQV+RI+ESAKNRQ+IGLKIILAQEEERKR Sbjct: 121 RVRNVENSVERAESIGSQMGVVLEYLSGELGQVSRIIESAKNRQVIGLKIILAQEEERKR 180 Query: 186 IAREIHDGPAQMLANLVLRTEIVERMLVKQEFGLVQDEVLDLKGQVRYSLEEMRKVIFNL 245 I+REIHDGPAQ+LANLVLRTEIVERML KQEF +VQDE++DLKGQVR SLEEMRKVIFNL Sbjct: 181 ISREIHDGPAQLLANLVLRTEIVERMLAKQEFKMVQDEIVDLKGQVRSSLEEMRKVIFNL 240 Query: 246 RPMALDDLGLIPTLRKYVHDYEEKTKIRTSFETRGKEHRLSSAMEAAVYRLVQEALSNAA 305 RPMALDDLGLIPTLRKYVHDYEEK+KIRT FETRGKEHRLSSAMEAA+YRLVQEALSNAA Sbjct: 241 RPMALDDLGLIPTLRKYVHDYEEKSKIRTIFETRGKEHRLSSAMEAAIYRLVQEALSNAA 300 Query: 306 KHAFPSYVLVEITYQAQLIKIVVKDNGLGFNVKKTSEQANRESFGLVGMRERVELLEGRM 365 KHA+P+YVLVEITYQAQL+KIVV+DNGLGFNV EQ +++ FGL+GMRERVELLEGRM Sbjct: 301 KHAYPTYVLVEITYQAQLVKIVVQDNGLGFNV-DLLEQKSKDHFGLIGMRERVELLEGRM 359 Query: 366 EILSAENQGTTIVIHIPTNVEKGKE 390 EI SAENQGT IVIHIPTNVEK KE Sbjct: 360 EIDSAENQGTKIVIHIPTNVEKRKE 384 >B1DEC1.1 B1DEC1_9BACL Histidine kinase Length = 386 Score = 505 bits (1301), Expect = e-141, Method: Compositional matrix adjust. Identities = 249/379 (65%), Positives = 312/379 (82%), Gaps = 1/379 (0%) Query: 13 IDRVIKNTIDVMESSKYQIFEILQVARDELVALTKELQRVMEETDETLQKVDKLELQYHR 72 I+RVI N I VME SK QI+EI + AR EL +L +EL+ V+ ET T+++VD+LE Y R Sbjct: 8 INRVITNAISVMEDSKLQIYEICESARQELESLGQELEIVLAETLTTIERVDQLEQDYRR 67 Query: 73 SRIRLTEVSRDFVRYSEKDIRVAYEKATELQLELMMTREREVYLRSRRDELQMRVRSVEN 132 +RIRLTEVS+DFVRY E+DI+ AYEKAT++QL+LM+ RE+E YL++RRD+LQ RVR+VE Sbjct: 68 ARIRLTEVSKDFVRYKEEDIKTAYEKATQIQLDLMVYREKEAYLKTRRDDLQKRVRNVEK 127 Query: 133 SVERAESIGSQMSVVLEYLSGELGQVTRIVESAKNRQMIGLKIILAQEEERKRIAREIHD 192 S+ERAE+I SQ++VVL+YLSG+L QV+RI+ESAK RQMIGLKII+AQEEERKRI+REIHD Sbjct: 128 SIERAETIASQINVVLQYLSGDLNQVSRILESAKTRQMIGLKIIVAQEEERKRISREIHD 187 Query: 193 GPAQMLANLVLRTEIVERMLVKQEFGLVQDEVLDLKGQVRYSLEEMRKVIFNLRPMALDD 252 GPAQ LAN+VLRTEIVERML+K+EF +VQDE+LDLKGQVR LEE+RK+IFNLRPMALDD Sbjct: 188 GPAQSLANIVLRTEIVERMLLKKEFQMVQDELLDLKGQVRSGLEEIRKIIFNLRPMALDD 247 Query: 253 LGLIPTLRKYVHDYEEKTKIRTSFETRGKEHRLSSAMEAAVYRLVQEALSNAAKHAFPSY 312 LGL+P LRK+ HD+E+KTKIRT FE GKE R+ SAMEA V+RLVQEA +NA KHA S+ Sbjct: 248 LGLVPALRKFAHDFEDKTKIRTIFELSGKEVRMPSAMEAGVFRLVQEAFTNAQKHARASF 307 Query: 313 VLVEITYQAQLIKIVVKDNGLGFNVKKTSEQA-NRESFGLVGMRERVELLEGRMEILSAE 371 V +E+ + + + I + DNG+GF+ ++ A N SFGL+GMRERV+LLEGRMEI S Sbjct: 308 VTLEVAFTTETVTIAIVDNGIGFHSEQAEAHAQNNSSFGLIGMRERVDLLEGRMEIDSKL 367 Query: 372 NQGTTIVIHIPTNVEKGKE 390 QGT I+I+IP VE KE Sbjct: 368 GQGTKIIINIPITVESRKE 386 >C0Z731.1 C0Z731_BREBN Two-component sensor histidine kinase EC=2.7.3.- Length = 380 Score = 355 bits (912), Expect = 3e-96, Method: Compositional matrix adjust. Identities = 187/370 (50%), Positives = 268/370 (72%), Gaps = 2/370 (0%) Query: 13 IDRVIKNTIDVMESSKYQIFEILQVARDELVALTKELQRVMEETDETLQKVDKLELQYHR 72 +D VIK TI+ +E+SK QIF+I + AR +L EL + +E + +QKVD LEL Y + Sbjct: 10 LDGVIKRTIETVETSKTQIFDIAENARQHGNSLKFELHELRQEISKVIQKVDALELAYRK 69 Query: 73 SRIRLTEVSRDFVRYSEKDIRVAYEKATELQLELMMTREREVYLRSRRDELQMRVRSVEN 132 SR +L VSR+F Y+E+DIRVAYE+A+ +Q+EL + RERE L+ RR++L+ ++R++E Sbjct: 70 SRNKLVLVSRNFHMYTEEDIRVAYEEASRIQVELSIYRERENNLKRRRNDLERQLRTLEE 129 Query: 133 SVERAESIGSQMSVVLEYLSGELGQVTRIVESAKNRQMIGLKIILAQEEERKRIAREIHD 192 ++ RAE + SQM VVL YL+G+L ++ +ESAK Q++GLK+I AQEEERKR+AREIHD Sbjct: 130 TIVRAEKLVSQMGVVLGYLTGDLSKIGEALESAKQHQLMGLKVIQAQEEERKRVAREIHD 189 Query: 193 GPAQMLANLVLRTEIVERMLVKQEFGLVQDEVLDLKGQVRYSLEEMRKVIFNLRPMALDD 252 GPAQ +AN+VLR+EIVERML + Q E+ +LK VR SL ++R++IF+LRPMALDD Sbjct: 190 GPAQSMANVVLRSEIVERMLKNERILEAQMELHELKEMVRLSLADVRRIIFDLRPMALDD 249 Query: 253 LGLIPTLRKYVHDYEEKTKIRTSFETRGKEHRLSSAMEAAVYRLVQEALSNAAKHAFPSY 312 LGL+PTL+KY+ EE+ + G E L S+++AA++RLVQE L+N KHA + Sbjct: 250 LGLVPTLQKYIQTCEERIQSSIDLVVFGVEPPLRSSVKAAIFRLVQECLNNVEKHAGATT 309 Query: 313 VLVEITYQAQLIKIVVKDNGLGFNVKKTSEQANRESFGLVGMRERVELLEGRMEILSAEN 372 V V++ + + +VVKD+G+GF+++K AN SFGL+GMRERV+LLEG +E+ SA Sbjct: 310 VQVKLEFVQDSLSLVVKDDGVGFDLEK--RMANGNSFGLLGMRERVQLLEGSVELQSAPG 367 Query: 373 QGTTIVIHIP 382 +GT ++ IP Sbjct: 368 EGTKVIFQIP 377 >A5D2A9.1 A5D2A9_PELTS Sensor protein DegS and signal transduction histidine kinase Length = 378 Score = 332 bits (850), Expect = 5e-89, Method: Compositional matrix adjust. Identities = 169/377 (44%), Positives = 260/377 (68%), Gaps = 1/377 (0%) Query: 8 FQADAIDRVIKNTIDVMESSKYQIFEILQVARDELVALTKELQRVMEETDETLQKVDKLE 67 F+ A+DR+IK T++V+E SK QI+ I + +R E++ +T+EL + E +E + VD L Sbjct: 2 FEVHALDRIIKETLEVIERSKIQIYGIAENSRAEMLRITEELDEIKNEVNELINYVDSLS 61 Query: 68 LQYHRSRIRLTEVSRDFVRYSEKDIRVAYEKATELQLELMMTREREVYLRSRRDELQMRV 127 ++ ++RIRL EVS+DF +Y+EKDI+ AY+KA LQ+EL+ +ERE LR RRD L++ + Sbjct: 62 IEEKKARIRLAEVSKDFQQYTEKDIKEAYDKAYALQVELIKAKEREKLLRYRRDHLEISL 121 Query: 128 RSVENSVERAESIGSQMSVVLEYLSGELGQVTRIVESAKNRQMIGLKIILAQEEERKRIA 187 R ++N+V+RAE + SQ+S+V YLSGEL + + + Q + L II AQEEERKR+A Sbjct: 122 RRLKNTVQRAEKLVSQVSIVFNYLSGELQDLNSKIGELQQAQQMALSIIKAQEEERKRVA 181 Query: 188 REIHDGPAQMLANLVLRTEIVERMLVKQEFGLVQDEVLDLKGQVRYSLEEMRKVIFNLRP 247 REIHDGPAQ +AN+V+R E ++L V++E+L L+ VR L ++RK+IF+LRP Sbjct: 182 REIHDGPAQSMANIVMRAEYCLKLL-DMNPAKVREELLALQDLVRMGLADVRKIIFDLRP 240 Query: 248 MALDDLGLIPTLRKYVHDYEEKTKIRTSFETRGKEHRLSSAMEAAVYRLVQEALSNAAKH 307 M LDDLGL P +++Y+ DY+E+ ++ F G++ RL S++E A++R++QEA++N KH Sbjct: 241 MLLDDLGLAPAIKRYLADYKEQYGLQVDFLFFGQQQRLDSSIEVALFRVIQEAVTNIRKH 300 Query: 308 AFPSYVLVEITYQAQLIKIVVKDNGLGFNVKKTSEQANRESFGLVGMRERVELLEGRMEI 367 A LV++ + + I +KD+G GF++ R+ +GLVGMRERV+LL G + I Sbjct: 301 ARAKNALVKMEILQKKVNIHIKDDGKGFDLDSVMADRERDGYGLVGMRERVQLLRGEINI 360 Query: 368 LSAENQGTTIVIHIPTN 384 ++A QGT+I I +P + Sbjct: 361 ITAPGQGTSISISVPLD 377 >B7GL15.1 B7GL15_ANOFW Signal transduction histidine kinase Length = 381 Score = 325 bits (834), Expect = 3e-87, Method: Compositional matrix adjust. Identities = 171/379 (45%), Positives = 259/379 (68%), Gaps = 4/379 (1%) Query: 4 ASVEFQADAIDRVIKNTIDVMESSKYQIFEILQVARDELVALTKELQRVMEETDETLQKV 63 ++ + A +DR++K I+ ++ SK +IF I + +R E L +EL+R EE + + Sbjct: 5 SNKKMNAKELDRIVKKMIETVDQSKAEIFHISEQSRKEHERLLEELRRTKEEVQRVISQT 64 Query: 64 DKLELQYHRSRIRLTEVSRDFVRYSEKDIRVAYEKATELQLELMMTREREVYLRSRRDEL 123 D LE++ +R RL++VS++F RY+E +IR AYEKA LQ+EL M +E+E LR RRDEL Sbjct: 65 DDLEMKTRLARQRLSDVSKNFSRYTEAEIRQAYEKAHALQMELAMAQEKEKQLRQRRDEL 124 Query: 124 QMRVRSVENSVERAESIGSQMSVVLEYLSGELGQVTRIVESAKNRQMIGLKIILAQEEER 183 + R+ +V+ ++ERA+ + Q++VVL YL+G+ +++ + A +Q GL+II AQEEER Sbjct: 125 ERRLAAVKETIERADHLIGQVTVVLNYLNGDFRELSEFITGANEKQEFGLRIIEAQEEER 184 Query: 184 KRIAREIHDGPAQMLANLVLRTEIVERMLVKQEFGLVQDEVLDLKGQVRYSLEEMRKVIF 243 KR++REIHDGPAQMLAN+++R++++ER+ ++ E+ DLK VR +L E+R++I+ Sbjct: 185 KRLSREIHDGPAQMLANVIMRSDLIERIYRERGAEEAIREMRDLKKMVRSALYEVRRIIY 244 Query: 244 NLRPMALDDLGLIPTLRKYVHDYEEKTKIRTSFETRGKEHRLSSAMEAAVYRLVQEALSN 303 +LRPMALDDLGLIPTL+KY+ EE K +F G+ RLS E A++RLVQEA+ N Sbjct: 245 DLRPMALDDLGLIPTLKKYLQTIEEYHKKTITFTYIGEVRRLSDRFEVAIFRLVQEAVQN 304 Query: 304 AAKHAFPSYVLVEITYQAQLIKIVVKDNGLGFNVKKTSEQANRESFGLVGMRERVELLEG 363 A KHA + V+I + + IVVKD+G GFN + ++A FGL+GMRER+E LEG Sbjct: 305 ALKHAEAKEIQVKIELKKDHLLIVVKDDGKGFNPNEKKDKA----FGLIGMRERIEWLEG 360 Query: 364 RMEILSAENQGTTIVIHIP 382 ++ + S +GT + +HIP Sbjct: 361 KLHLYSQLGRGTIVTMHIP 379 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.317 0.133 0.344 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,825,922,294 Number of extensions: 67563908 Number of successful extensions: 326050 Number of sequences better than 1.0e-05: 3701 Number of HSP's gapped: 319939 Number of HSP's successfully gapped: 3701 Length of query: 390 Length of database: 3,060,696,203 Length adjustment: 138 Effective length of query: 252 Effective length of database: 1,760,596,133 Effective search space: 443670225516 Effective search space used: 443670225516 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)