BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0327.0010.p (388 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K7I2.1 B3K7I2_9BACI Polysaccharide pyruvyl transferase 435 e-120 B1DED4.1 B1DED4_9BACL Polysaccharide pyruvyl transferase 343 2e-92 B7GMA3.1 B7GMA3_ANOFW Polysaccharide pyruvyl transferase family ... 220 2e-55 C2YMP1.1 C2YMP1_BACCE Pyruvyl-transferase 210 2e-52 Q9L471.2 Q9L471_BACAN Hypothetical pyruvyl-transferase 209 5e-52 >B3K7I2.1 B3K7I2_9BACI Polysaccharide pyruvyl transferase Length = 412 Score = 435 bits (1118), Expect = e-120, Method: Compositional matrix adjust. Identities = 222/414 (53%), Positives = 282/414 (68%), Gaps = 28/414 (6%) Query: 1 MVAASQKIIISGYYGFRNSGDEAVLQSILNALQKHSLATGTVIEPVVLSIDPEWTTATYG 60 MV+ S+ I+ISGYYGF+NSGDEAVL+SIL AL++ A G I PVVLSIDPEWT+A YG Sbjct: 1 MVSTSKTIVISGYYGFKNSGDEAVLKSILTALEQEGQAAGVDINPVVLSIDPEWTSAAYG 60 Query: 61 VKAVHRMKLSEVRXXXXXXXXXXXXXXXXXQDVTGSKTIPYYLGIIKLAQWMGKPTFIYA 120 VK+VHRMKL EVR QD T KTIPYYLG++K+AQW+GKPTFIYA Sbjct: 61 VKSVHRMKLGEVRQAIKDSDGLISGGGSLLQDATSPKTIPYYLGVLKIAQWLGKPTFIYA 120 Query: 121 QGIGPVNRKLFHPLIKAVFRKCSYISVRDEQSRDLLQSMGLVGNKIDVVPDPVMGLSLPE 180 QG+GPVNRKLF+P+I+++FRKC+YISVRD QS +LLQSMG+ G + VVPDPVMGL LP Sbjct: 121 QGVGPVNRKLFYPMIRSIFRKCTYISVRDMQSGELLQSMGISGEAVHVVPDPVMGLPLP- 179 Query: 181 DGQG-------------------SSTEAGGESSPQ---KTPLSRPD----LPVVGVSVRF 214 +G+G SS E ESS + + LS D LPV+G+SVR+ Sbjct: 180 NGEGIHRDSRLGSALKENEAASVSSIEEQRESSEEPVKPSSLSSGDTKGSLPVIGISVRY 239 Query: 215 WEQSREELDAVADGLLQASARTPLHVRFLPFHHPSDHEPSRYIMEKLEKGMSQNGGVISI 274 W R+EL AVA+GL + + P+H+RFLPFH P D E S++I + + +S+NG +S Sbjct: 240 WHPERKELKAVAEGLKALAEQRPVHLRFLPFHQPDDIEASQFIADAM-GDVSKNGSALSF 298 Query: 275 CEDAEHPQQMLREVGTCDLLIGMRLHSLIYAAGRRVPLIGISYDPKIDHFLERVGCRPIG 334 D + PQ MLREV CD+++GMRLHSLIYAA + VPL+GISYDPKI+HFL R+G P+G Sbjct: 299 YRDEKQPQDMLREVSRCDMILGMRLHSLIYAANQSVPLLGISYDPKINHFLRRLGSEPVG 358 Query: 335 DTSSLDGGKLAAEVLQLLESGDEWKREREPLIASLVQEAEAPARQIIEYFRRKG 388 + SLD L ++L+LL+ WK E IA L +EA PAR I +Y R +G Sbjct: 359 SSESLDPSVLKDQMLKLLDDAAAWKAEHGEQIALLKREAAMPARHIAQYLRHRG 412 >B1DED4.1 B1DED4_9BACL Polysaccharide pyruvyl transferase Length = 385 Score = 343 bits (880), Expect = 2e-92, Method: Compositional matrix adjust. Identities = 177/363 (48%), Positives = 227/363 (62%), Gaps = 9/363 (2%) Query: 7 KIIISGYYGFRNSGDEAVLQSILNALQKHSLATGTVIEPVVLSIDPEWTTATYGVKAVHR 66 +I+ISGYYGFRNSGDEAVL+SIL AL + A G I+PVVLS DP WT+ YGV+AV R Sbjct: 11 RIVISGYYGFRNSGDEAVLRSILLALAEQGEANGVQIQPVVLSADPAWTSRMYGVEAVRR 70 Query: 67 MKLSEVRXXXXXXXXXXXXXXXXXQDVTGSKTIPYYLGIIKLAQWMGKPTFIYAQGIGPV 126 M +V QD TG+KTIPYY GIIKLAQW+GKPTFIY+QGIGPV Sbjct: 71 MHPKDVLNALRASDGLISGGGSLLQDATGAKTIPYYTGIIKLAQWLGKPTFIYSQGIGPV 130 Query: 127 NRKLFHPLIKAVFRKCSYISVRDEQSRDLLQSMGLVGNKIDVVPDPVMGLSLPEDGQGSS 186 NR+ PLI++ RK +Y+SVRD +S +LL+ +G+ +I+VVPDPVMGL LP Sbjct: 131 NRRWMDPLIRSSMRKSAYVSVRDAESAELLKRIGVAAGRIEVVPDPVMGLPLPAGAAAGR 190 Query: 187 TEAGGESSPQKTPLSRPDLPVVGVSVRFWEQSREELDAVADGLLQASARTPLHVRFLPFH 246 A + +PV+GVS+R W + E+L AD L + ++R P+ +RFLPFH Sbjct: 191 AAAAAGEAAA--------VPVLGVSLRHWRKDGEDLGRAADALAELASRRPVRLRFLPFH 242 Query: 247 HPSDHEPSRYIMEKLEKGMSQNGGVISICEDAEHPQQMLREVGTCDLLIGMRLHSLIYAA 306 P D E S+ +ME+L G G + + PQQML EV CD L GMRLH+LIYAA Sbjct: 243 TPDDAEASKLVMERL-GGRLAGGSSAELAAPGDDPQQMLLEVSRCDALFGMRLHALIYAA 301 Query: 307 GRRVPLIGISYDPKIDHFLERVGCRPIGDTSSLDGGKLAAEVLQLLESGDEWKREREPLI 366 + VP++G+SYDPKID FL R+G P+G T LD A +LL+ + WK + I Sbjct: 302 NQMVPMLGLSYDPKIDQFLHRLGLTPVGTTEQLDPIAFADSAQRLLDDANGWKEKHREAI 361 Query: 367 ASL 369 L Sbjct: 362 ERL 364 >B7GMA3.1 B7GMA3_ANOFW Polysaccharide pyruvyl transferase family protein Length = 368 Score = 220 bits (561), Expect = 2e-55, Method: Compositional matrix adjust. Identities = 144/392 (36%), Positives = 210/392 (53%), Gaps = 41/392 (10%) Query: 5 SQKIIISGYYGFRNSGDEAVLQSILNALQKHSLATGTVIEPVVLSIDPEWTTATYGVKAV 64 + I++SGYYGF N GDEA+L +I+ AL+ H ++ VLS DPE+T TYGV AV Sbjct: 8 TMNIVLSGYYGFHNVGDEAILYAIIQALRAHD----PNVQVTVLSNDPEFTKQTYGVHAV 63 Query: 65 HRMKLSEVRXXXXXXXXXXXXXXXXXQDVTGSKTIPYYLGIIKLAQWMGKPTFIYAQGIG 124 +R ++ ++ QD TG ++IPYY GI+++A+W+GKP +YAQG+G Sbjct: 64 NRWRIGDIVRALRQSDGLISGGGSLLQDQTGWRSIPYYTGIMQIAKWLGKPVMVYAQGMG 123 Query: 125 PVNRKLFHPLIKAVFRKCSYISVRDEQSRDLLQSMGLVGNKIDVVPDPVMGLSLPEDGQG 184 PVN+K L+K + ++VRDE S LL+++G V I +VPDPVMGL P Sbjct: 124 PVNKKQNQWLMKWTLNQVDRLTVRDEPSAQLLKALG-VTKPITIVPDPVMGLKPP----- 177 Query: 185 SSTEAGGESSP----QKTPLSRPDLPVVGVSVRFWEQSR--EELDAVADGLLQASARTPL 238 ++P Q+ +R PVV VSVR W + R EE AVA L S Sbjct: 178 --------TTPTVWWQQQSFTR---PVVAVSVRDWPRGRDFEEKIAVA---LDRSIEAGF 223 Query: 239 HVRFLPFHHPSDHEPSRYIMEKLEKGMSQNGGVISICEDAEHPQQMLREVGTCDLLIGMR 298 V F+P H D + SR ++ M + V++ DA ++M + + DLL+GMR Sbjct: 224 DVVFIPMHGHHDDQTSRRVL-----AMMKERAVVA-PYDASIEEKMAM-IASADLLVGMR 276 Query: 299 LHSLIYAAGRRVPLIGISYDPKIDHFLERVGCRPIG---DTSSLDGGKLAAEVLQLLESG 355 LH+LI+AA P + +SYDPKID F + +PI + DG L + + Q + Sbjct: 277 LHALIFAAVVHTPFVALSYDPKIDAFANQAN-QPIFAHVEDDRWDGEALFSYIQQCMNKR 335 Query: 356 DEWKREREPLIASLVQEAEAPARQIIEYFRRK 387 +E K +A L Q A+ A++ + FR K Sbjct: 336 EERKEAMIQAVAPLQQTAQQTAKEALALFRNK 367 >C2YMP1.1 C2YMP1_BACCE Pyruvyl-transferase Length = 367 Score = 210 bits (535), Expect = 2e-52, Method: Compositional matrix adjust. Identities = 138/374 (36%), Positives = 199/374 (53%), Gaps = 31/374 (8%) Query: 7 KIIISGYYGFRNSGDEAVLQSILNALQKHSLATGTVIEPVVLSIDPEWTTATYGVKAVHR 66 ++++SGYYGF N GDEA+LQSI+ AL + +E VVLS DP++T YGV+AV+R Sbjct: 2 RLVLSGYYGFYNVGDEAILQSIIKALHEED----PTLELVVLSNDPDYTRKMYGVEAVNR 57 Query: 67 MKLSEVRXXXXXXXXXXXXXXXXXQDVTGSKTIPYYLGIIKLAQWMGKPTFIYAQGIGPV 126 + + QD T K+I YY GI+++A+++ KP +IYAQGIGP+ Sbjct: 58 WDIRAIYKEIKKSNGLISGGGSLLQDKTSIKSILYYTGIMRIARFLKKPYYIYAQGIGPI 117 Query: 127 NRKLFHPLIKAVFRKCSYISVRDEQSRDLLQSMGLVGNKIDVVPDPVMGLSLPEDGQGSS 186 ++ L+K K +YISVRDE S L+ MG + I++VPDPV+ PE G Sbjct: 118 TKRQNRLLVKWQVSKAAYISVRDEDSFLYLKDMG-IKKDIELVPDPVLACQ-PE---GMK 172 Query: 187 TEAGGESSPQKTPLSRPDLPVVGVSVRFWEQSREELDAVADGLLQASARTPLHVRFLPFH 246 ++ + S Q V+ VSVR+W+ + + +AD L Q R H+ F+P H Sbjct: 173 SDWLQKHSIQG--------KVIAVSVRYWDAKEDYMKKLADTLKQFK-RDGYHILFVPMH 223 Query: 247 HPSDHEPSRYIMEKLEKGMSQNGGVISICEDAEHPQQMLREVGTCDLLIGMRLHSLIYAA 306 P D SR I+ M + ++ D +L E C LLIGMRLH+LI +A Sbjct: 224 GPFDQNASRDIINL----MGEEAHMLPYKLDIHEKISILSE---CSLLIGMRLHALILSA 276 Query: 307 GRRVPLIGISYDPKIDHFLERVGCRPIGDTSSLDGGKLAAEVLQLLESGDEWKREREPLI 366 +P++GISYDPKID FL++V IG+ G AE L + ++E + Sbjct: 277 VANIPMVGISYDPKIDSFLQQVNQPIIGNVD----GDWTAET--LYNVATKQLEQKEYVQ 330 Query: 367 ASLVQEAEAPARQI 380 A+L Q E QI Sbjct: 331 ATLEQRVEELREQI 344 >Q9L471.2 Q9L471_BACAN Hypothetical pyruvyl-transferase Length = 367 Score = 209 bits (531), Expect = 5e-52, Method: Compositional matrix adjust. Identities = 140/392 (35%), Positives = 206/392 (52%), Gaps = 39/392 (9%) Query: 7 KIIISGYYGFRNSGDEAVLQSILNALQKHSLATGTVIEPVVLSIDPEWTTATYGVKAVHR 66 ++++SGYYGF N GDEA+LQSI+ AL + +E VVLS DP++T YGV+AV+R Sbjct: 2 RLVLSGYYGFYNVGDEAILQSIIKALHEED----PTLELVVLSNDPDYTRKMYGVEAVNR 57 Query: 67 MKLSEVRXXXXXXXXXXXXXXXXXQDVTGSKTIPYYLGIIKLAQWMGKPTFIYAQGIGPV 126 + + QD T K+I YY GI+++A+++ KP +IYAQGIGP+ Sbjct: 58 WDIRAIYKEIKRSNGLISGGGSLLQDKTSIKSILYYTGIMRIARFLKKPYYIYAQGIGPI 117 Query: 127 NRKLFHPLIKAVFRKCSYISVRDEQSRDLLQSMGLVGNKIDVVPDPVMGLSLPEDGQGSS 186 ++ L+K K YISVRDE S L+ +G + I++VPDPV+ PE G Sbjct: 118 TKRQNRLLVKWQVSKAEYISVRDEDSFLYLKEIG-IKKDIELVPDPVLACQ-PE---GMK 172 Query: 187 TEAGGESSPQKTPLSRPDLPVVGVSVRFWEQSREELDAVADGLLQASARTPLHVRFLPFH 246 +E + S Q V+ VSVR+W+ + + +AD L Q R H+ F+P H Sbjct: 173 SEWLQKHSIQGK--------VIAVSVRYWDAKEDYMKKLADTLKQLK-RDGYHILFVPMH 223 Query: 247 HPSDHEPSRYIMEKLEKGMSQNGGVISICEDAEHPQQMLREVGTCDLLIGMRLHSLIYAA 306 P D SR I+ M + ++ D +L E C LLIGMRLH+LI +A Sbjct: 224 GPFDQNASRDIINL----MGEEAHMLPYKLDIHEKISILSE---CSLLIGMRLHALILSA 276 Query: 307 GRRVPLIGISYDPKIDHFLERVGCRPIGDTSSLDGGKLAAEVL-----QLLESGDEWKRE 361 +P++GISYDPKID FL++V IG+ G AE L + LE + + Sbjct: 277 VANIPMVGISYDPKIDSFLQQVNQPIIGNVD----GDWTAETLYNVATKQLEQKEYVQET 332 Query: 362 REPLIASLVQEAEAPARQII-----EYFRRKG 388 E + L ++ +R II + F+++G Sbjct: 333 LEQRVEELREQISTASRYIISDLNSKEFKKRG 364 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.318 0.136 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,276,736,839 Number of extensions: 94452330 Number of successful extensions: 230722 Number of sequences better than 1.0e-05: 182 Number of HSP's gapped: 230039 Number of HSP's successfully gapped: 182 Length of query: 388 Length of database: 3,060,696,203 Length adjustment: 138 Effective length of query: 250 Effective length of database: 1,760,596,133 Effective search space: 440149033250 Effective search space used: 440149033250 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)