BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0327.0005.p (271 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K7I7.1 B3K7I7_9BACI Putative uncharacterized protein 280 1e-73 B1DEE0.1 B1DEE0_9BACL Putative uncharacterized protein 194 5e-48 B7GMD1.1 B7GMD1_ANOFW Flagellar hook-basal body protein 152 4e-35 Q8EMA1.1 Q8EMA1_OCEIH Flagellar hook-basal body protein 149 3e-34 C4G061.1 C4G061_ABIDE Putative uncharacterized protein 148 6e-34 >B3K7I7.1 B3K7I7_9BACI Putative uncharacterized protein Length = 275 Score = 280 bits (716), Expect = 1e-73, Method: Compositional matrix adjust. Identities = 138/275 (50%), Positives = 188/275 (68%), Gaps = 4/275 (1%) Query: 1 MNNSTIGAAVSMSSLQQRLDIIADNVANMNTDGYKSKQGSFEDVLTRVQQQSKDYDQPGR 60 MNNS I A VSM++LQQRLD+IADN+AN+NT GYK K+ SFEDVLT+VQ KD+D+ GR Sbjct: 1 MNNSMISAMVSMNALQQRLDVIADNMANVNTAGYKKKEASFEDVLTQVQGHHKDFDRTGR 60 Query: 61 STPLGFNIGFGVRVPTVTTNWEEGPLKETGNPTDLAIQGNGLFGVQVNGATAYTRQGDFH 120 +TPLG+ +G+G+R+ +V N E+G +KETGNP DL I G +F VQ +GA YTR+G FH Sbjct: 61 ATPLGYTLGYGMRLSSVVPNMEQGAMKETGNPLDLMIDGQAMFAVQGDGAIGYTREGGFH 120 Query: 121 FVPDSANPDRMMLVDNTGNPVLNTQGNPLTVPADVSAAIDENGRVLTKTAENAPARVAGT 180 PD+ NP+ ++LV++ G PVL+ NP+++PA AI+E G+++ +T T Sbjct: 121 VSPDARNPNNLLLVNSQGYPVLDVNDNPISLPAKGKVAINERGQIIVETPTGGRTAPYAT 180 Query: 181 IMIVEPLSKSALQAVDGNFFVLADGVTAQQAFK----GAGEPSTIGVRSGYLEQSNVDLS 236 + +VE L AV N FVL +G+ F GEP V SG++EQSNV+L+ Sbjct: 181 LKLVELERPEGLVAVGDNLFVLGNGLAEGDVFDTTPPAPGEPPKSKVLSGFVEQSNVNLT 240 Query: 237 KEIAEMMQIQRTYQLAARALTSSDQMLGLANNMRG 271 E+ +MM++QR YQL ARAL+SSD M+ LAN MRG Sbjct: 241 DELTKMMEVQRAYQLTARALSSSDTMMNLANTMRG 275 >B1DEE0.1 B1DEE0_9BACL Putative uncharacterized protein Length = 275 Score = 194 bits (494), Expect = 5e-48, Method: Compositional matrix adjust. Identities = 119/278 (42%), Positives = 170/278 (61%), Gaps = 12/278 (4%) Query: 1 MNNSTIGAAVSMSSLQQRLDIIADNVANMNTDGYKSKQGSFEDVLTRVQQQSKDYDQPGR 60 MN+S I A+VSM++LQQ+LD++ADN+AN+NT GYK K+ +FED+L ++Q ++QPGR Sbjct: 1 MNSSMINASVSMNALQQKLDMLADNIANVNTVGYKRKEATFEDLLNNTRKQPDAFNQPGR 60 Query: 61 STPLGFNIGFGVRVPTVTTNWEEGPLKETGNPTDLAIQGNGLFGVQV--NGATAYTRQGD 118 T L FN G+G R+ + N ++G +K T TDLAI+G+ LF V V NG AYTR G Sbjct: 61 LTSLNFNQGWGSRLAMIQPNLQQGTIKTTEKDTDLAIEGDALFEVAVNANGDRAYTRNGA 120 Query: 119 FHFVPDSANPDRMMLVDNTGNPVL-NTQG--NPLTVPADVSAAIDENGRVLTKTAENAPA 175 +AN D +L G PV+ NT PL VP+ ++ +G A + Sbjct: 121 LQLTV-AANGD-TILATPEGYPVVANTPAGEGPLVVPSGYQLRVNADGTA-EGVATDGST 177 Query: 176 RVAGTIMIVEPLSKSALQAVDGNFFVLADGVTAQQAFKGA---GEPSTIGVRSGYLEQSN 232 G+I +V+ +AL V N + +ADG+ + GE + I +R G LEQSN Sbjct: 178 IELGSIKLVQATRPAALTLVADNLYEIADGINVADVLQNVTPNGE-NGIALRQGALEQSN 236 Query: 233 VDLSKEIAEMMQIQRTYQLAARALTSSDQMLGLANNMR 270 V+L E+ E++ +QR YQ+AARALTSSD M+GLAN++R Sbjct: 237 VNLGDEMTELINVQRAYQMAARALTSSDTMMGLANSLR 274 >B7GMD1.1 B7GMD1_ANOFW Flagellar hook-basal body protein Length = 274 Score = 152 bits (383), Expect = 4e-35, Method: Compositional matrix adjust. Identities = 100/276 (36%), Positives = 150/276 (54%), Gaps = 9/276 (3%) Query: 1 MNNSTIGAAVSMSSLQQRLDIIADNVANMNTDGYKSKQGSFEDVLTRVQQQSKDYDQPGR 60 M S I AA +M+ LQQ+LD+I+ N+AN+NT G+K + F ++L + D+ R Sbjct: 1 MLRSMITAANTMNQLQQQLDVISHNIANVNTTGFKKRDAVFGELLAQQFTNLPTTDEAPR 60 Query: 61 STPLGFNIGFGVRVPTVTTNWEEGPLKETGNPTDLAIQGNG-LFGVQVNGATAYTRQGDF 119 TP G +G G +V + ++G + +T P DLA+ G F + V+GA YTR G F Sbjct: 61 LTPAGVRLGVGAKVASTYMQMKQGSVVKTDRPLDLALTKEGQFFRIFVDGAIQYTRDGAF 120 Query: 120 HFVPDSANPDRMMLVDNTGNPVLNTQGNPLTVP---ADVSAAIDENGRVLTKTAENAPAR 176 + P + NPD +M+V G VLN P+TV D+ + +NG + A N Sbjct: 121 YLSPSANNPDELMIVTADGYAVLNENDEPITVRDGFKDIQ--VTQNG-TMQFIAPNGNIM 177 Query: 177 VAGTIMIVEPLSKSALQAVDGNFFVLADGVTAQQA-FKGAG-EPSTIGVRSGYLEQSNVD 234 I + +QA+ N F +G+ + A AG + I ++ LEQSNV+ Sbjct: 178 QTVNIGVSYITRPQLMQALGDNRFAFPNGLNVEDATINLAGNNRNNISLQQYALEQSNVE 237 Query: 235 LSKEIAEMMQIQRTYQLAARALTSSDQMLGLANNMR 270 L+ E++E+M QR+YQL ARA+T SDQM+GL N +R Sbjct: 238 LATEMSELMITQRSYQLNARAITLSDQMMGLINGIR 273 >Q8EMA1.1 Q8EMA1_OCEIH Flagellar hook-basal body protein Length = 276 Score = 149 bits (376), Expect = 3e-34, Method: Compositional matrix adjust. Identities = 104/283 (36%), Positives = 152/283 (53%), Gaps = 21/283 (7%) Query: 1 MNNSTIGAAVSMSSLQQRLDIIADNVANMNTDGYKSKQGSFEDVLTRVQQQSKDYDQP-- 58 M+ I AAV+M+ LQ +LD+I +N+AN T GYKS+Q F +LT QQ + P Sbjct: 1 MSQMMIQAAVTMNQLQNKLDLIGNNMANSQTTGYKSRQAEFSSLLT---QQVNNLTDPAN 57 Query: 59 --GRSTPLGFNIGFGVRVPTVTTNWEEGPLKETGNPTDLAIQG-NGLFGVQV--NG--AT 111 GR TP G +G G R+ + N +G ++ET D+A+ N LF V+V NG T Sbjct: 58 AQGRLTPDGIRVGSGSRLGSTNMNLNQGAIQETNRDLDVALTSENLLFQVEVTENGQVGT 117 Query: 112 AYTRQGDFHFVPDSANPDRMMLVDNTGNPVLNTQGNPLTVPADVSAAIDENGRVLTKTAE 171 YTR G F+ P +MML + GNPV+ G L S I+E G++L + Sbjct: 118 RYTRDGSFYLSP--MENGQMMLSTSDGNPVIGNNGPILIQDGFDSIEINEYGQLLVE--R 173 Query: 172 NAPARVAGTIMIVEPLSKSALQAVDGNFFVL----ADGVTAQQAFKGAGEPSTIGVRSGY 227 N + + +VE + L++ N F L A G A++ + P T ++S Sbjct: 174 NGQQAIEDQLSVVEAIRPRLLESTGQNLFQLPNLEALGFNAEEIIQETN-PQTAILQSKA 232 Query: 228 LEQSNVDLSKEIAEMMQIQRTYQLAARALTSSDQMLGLANNMR 270 LEQSNVD+SKE+++++ QR YQ +R +T DQM+GL N +R Sbjct: 233 LEQSNVDISKEMSDLLMTQRLYQFNSRTITMGDQMMGLVNQIR 275 >C4G061.1 C4G061_ABIDE Putative uncharacterized protein Length = 274 Score = 148 bits (373), Expect = 6e-34, Method: Compositional matrix adjust. Identities = 101/281 (35%), Positives = 153/281 (54%), Gaps = 19/281 (6%) Query: 1 MNNSTIGAAVSMSSLQQRLDIIADNVANMNTDGYKSKQGSFEDVLTRVQQQSKDYDQPGR 60 M S AA M + Q LD I++N+AN+NT G+K++ F+ +L + Q ++K D G Sbjct: 1 MLRSLWSAASGMMAQQVNLDTISNNIANINTTGFKTQSMEFKSLLYQ-QLKTKSTDSEGN 59 Query: 61 STPLGFNIGFGVRVPTVTTNWEEGPLKETGNPTDLAIQGNGLFGVQVNGATAYTRQGDFH 120 P+G +G GVR ++T + +G +TG DL IQGNG F V+V TAYTR G Sbjct: 60 PKPIGVQVGLGVRNASITAKFTQGAALDTGEMLDLFIQGNGFFAVKVGEKTAYTRNGHMV 119 Query: 121 FVPDSANPDRMMLVDNTGNPVLNTQGNPLTV-----PADVSAAIDENGRVLTKTAENAPA 175 F S+ D L + GNP+L+T+G P+ + P D+S ID +G VL + + Sbjct: 120 F---SSVADGWELTTSDGNPILSTKGTPIKIDSKWDPKDIS--IDLSGNVLHRVDDKEAG 174 Query: 176 RVA----GTIMIVEPLSKSALQAVDGNFFVL--ADGVTAQQAFKGAGEPSTIGVRSGYLE 229 G I + + + + L G+ + A G A+ AG ++ ++SGYLE Sbjct: 175 TTKYDNFGKIALYQFNNPAGLNRSGGSLYYETPASG-EARSEDTDAGLNKSL-IKSGYLE 232 Query: 230 QSNVDLSKEIAEMMQIQRTYQLAARALTSSDQMLGLANNMR 270 SNV + E+ M+ QR YQL ++A+T+SD+ML ANN+R Sbjct: 233 GSNVSAADEMVNMIVTQRAYQLNSKAITASDEMLEQANNLR 273 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.313 0.129 0.361 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,587,565,722 Number of extensions: 69150617 Number of successful extensions: 156499 Number of sequences better than 1.0e-05: 2367 Number of HSP's gapped: 153463 Number of HSP's successfully gapped: 2421 Length of query: 271 Length of database: 3,060,696,203 Length adjustment: 133 Effective length of query: 138 Effective length of database: 1,807,701,208 Effective search space: 249462766704 Effective search space used: 249462766704 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 130 (54.7 bits)