BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0327.0004.p (291 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K7I8.1 B3K7I8_9BACI Putative uncharacterized protein 283 2e-74 B1DEE1.1 B1DEE1_9BACL Flagellar basal body rod protein 254 5e-66 C5D968.1 C5D968_9BACI Flagellar basal body rod protein 191 8e-47 Q9K6J1.1 Q9K6J1_BACHD Flagellar basal-body rod protein 189 2e-46 B4BP36.1 B4BP36_9BACI Flagellar basal body rod protein 189 4e-46 >B3K7I8.1 B3K7I8_9BACI Putative uncharacterized protein Length = 281 Score = 283 bits (723), Expect = 2e-74, Method: Compositional matrix adjust. Identities = 141/283 (49%), Positives = 198/283 (69%), Gaps = 6/283 (2%) Query: 13 VTQQRRHDTATQNIANLNTTGYKQVDSVSHAFPEVLISAMSDGRIKPVGR----MNTGVF 68 +TQ++RH+T TQNIAN+NTTGYK +SV +FPEVL+S + VGR NTGV+ Sbjct: 1 MTQEQRHNTVTQNIANVNTTGYKATNSVQRSFPEVLVSMIGGNESSNVGRKLGKFNTGVY 60 Query: 69 AEQSVSQYLQGDLIESGKPADFALSTDLQVADPATGANINFDGSGKYITPDGEVIYRPQA 128 AE+S+S Y +G L E+ K DFAL + V +PATG FD SG+Y+ +GEVIYRP+ Sbjct: 61 AEESISLYTEGALRETNKTTDFALVSSFTVQNPATGQAYPFDASGRYVDENGEVIYRPEG 120 Query: 129 FFTVQDNEGNQLYTRNGSFTVNAAGEVLSAGGFKVVDSNGNPLILTGSQDNLKVDGQGNL 188 FFTVQ+ G+ YTRNGSF V+ G +LS G++V+D+N P++LTG + V+ QG + Sbjct: 121 FFTVQNENGDVRYTRNGSFRVSPEGNLLSESGYQVLDNNNRPIVLTGPASDFTVNEQGEI 180 Query: 189 LNPATGLPSGTRLGISVVTKPQELVRDGNGVFHADDVENADIRFANGTDNLQVRQGYLEN 248 ++ A G +G LG+S+V++P L+R+G+GVF D + ++RF DN VRQG++E Sbjct: 181 MD-AAGRGTGIILGVSIVSQPHGLIREGHGVFRMAD-PDGEVRFMTPADNGVVRQGFIEG 238 Query: 249 SNVDATKVTVDMNAAYRAYEANQKVVQFYDSSLQKAVNEVGRI 291 SNVD T+ V++ AA RAYE NQK+VQ+YD +L+KAVNE+GR+ Sbjct: 239 SNVDPTQSMVELTAALRAYETNQKIVQYYDRTLEKAVNEIGRV 281 >B1DEE1.1 B1DEE1_9BACL Flagellar basal body rod protein Length = 288 Score = 254 bits (650), Expect = 5e-66, Method: Compositional matrix adjust. Identities = 144/296 (48%), Positives = 203/296 (68%), Gaps = 13/296 (4%) Query: 1 MLRGLYTAAAGMVTQQRRHDTATQNIANLNTTGYKQVDSVSHAFPEVLIS--AMSDGRIK 58 MLRGLYTAAAGM QQRRHDT T NI N+NT GYKQ +V+ +FPE+L+S + K Sbjct: 1 MLRGLYTAAAGMTAQQRRHDTITNNITNINTPGYKQTYAVTRSFPEMLLSLTGVDQKDEK 60 Query: 59 PVGRMNTGVFAEQSVSQYLQGDLIESGKPADFALSTDLQVADPATGANINFDGSGKYITP 118 +G+++TGVFAE+S++ +LQGDL+++GK +DFAL +++ V + FD SGKY++P Sbjct: 61 QIGKVSTGVFAEESLAIHLQGDLMQTGKSSDFALVSNIDVP------GMAFDASGKYVSP 114 Query: 119 DGEVIYRPQAFFTVQDNEGNQLYTRNGSFTVNAAGEVLSAGGFKVVDSNGNPLILTG--S 176 DG+V ++PQAFFT+QD G YTR GSFT+ + G +++ G V+ ++GNP+ S Sbjct: 115 DGDVTFQPQAFFTLQDKNGEIRYTRGGSFTLTSDGFLVAGDGSNVLGADGNPIQFPQGVS 174 Query: 177 QDNLKVDGQGNLLNPATGLPSGTRLGISVVTKPQELVRDGNGVFH-ADDVENADIRFANG 235 D L + ++ TG SG +L ++ + P EL R+G+G F A D ++A R Sbjct: 175 LDGLTLTADQRFVDAKTGADSGRQLLLTRIDNPNELAREGDGKFRLAGDAQDA--RPVVA 232 Query: 236 TDNLQVRQGYLENSNVDATKVTVDMNAAYRAYEANQKVVQFYDSSLQKAVNEVGRI 291 + ++VRQGY+E SNVDA + +VD+ AA RAYEANQKV+QFYD SL KAVN+VGR+ Sbjct: 233 GERVEVRQGYVERSNVDAAQSSVDLMAALRAYEANQKVIQFYDESLDKAVNQVGRV 288 >C5D968.1 C5D968_9BACI Flagellar basal body rod protein Length = 278 Score = 191 bits (484), Expect = 8e-47, Method: Compositional matrix adjust. Identities = 116/304 (38%), Positives = 171/304 (56%), Gaps = 40/304 (13%) Query: 1 MLRGLYTAAAGMVTQQRRHDTATQNIANLNTTGYKQVDSVSHAFPEVLISAMSDGRI--- 57 MLRG YTAA+GM+ QQRR + T NIAN NT GYK + AFPE+L+S + + Sbjct: 1 MLRGFYTAASGMMAQQRRIEMWTNNIANANTPGYKSDQAALRAFPELLLSRLDKATVPTK 60 Query: 58 -------KP-VGRMNTGVFAEQSVSQYLQGDLIESGKPADFALSTDLQVADPATGANINF 109 +P +G +NTGV+ ++ + + QGD+ E+G+P D AL D TG Sbjct: 61 TPRSFPLQPTIGSINTGVYMQELIPNFRQGDIKETGQPTDIALINGTLPVDAETGK---- 116 Query: 110 DGSGKYITPDGEVIYRPQAFFTVQDNEGNQLYTRNGSFTVNAAGEVLSAGGFKVVDSNGN 169 +G + FF VQ+ G+ YTRNG+FT+N G + + G+ V+D NG Sbjct: 117 ---------EGTL------FFIVQNENGDIRYTRNGNFTLNPQGFLTTNDGWYVLDENGR 161 Query: 170 PLILTGSQDNLKVDGQGNLLNPATGLPSGTRLGISVVTKPQELVRDGNGVFHADDVENAD 229 + L + N V+ G ++ T + R+ I+ P LV++GNG+F + EN Sbjct: 162 RIQLPST--NFAVNADGTIVANNTRI---ARINIAFAANPNTLVKEGNGLFRS---ENGV 213 Query: 230 IRFANGTDNLQ--VRQGYLENSNVDATKVTVDMNAAYRAYEANQKVVQFYDSSLQKAVNE 287 + A G N+ ++QG+LE +NVD + +M +AYRA+EANQK+VQ YD S+ KAVNE Sbjct: 214 LPSALGNPNITYTLKQGFLERANVDLNRAMTEMLSAYRAFEANQKIVQAYDRSMDKAVNE 273 Query: 288 VGRI 291 +GR+ Sbjct: 274 IGRL 277 >Q9K6J1.1 Q9K6J1_BACHD Flagellar basal-body rod protein Length = 274 Score = 189 bits (480), Expect = 2e-46, Method: Compositional matrix adjust. Identities = 115/297 (38%), Positives = 170/297 (57%), Gaps = 30/297 (10%) Query: 1 MLRGLYTAAAGMVTQQRRHDTATQNIANLNTTGYKQVDSVSHAFPEVLISAMSDGRIKP- 59 MLRGL+ AAAGM+TQQ+R + T N+AN+NT GYK + AFP +LI+AM+ G + Sbjct: 1 MLRGLWGAAAGMITQQQRQEMLTNNLANVNTPGYKADQAAIRAFPNMLIAAMNTGNVPKG 60 Query: 60 ----VGRMNTGVFAEQSVSQYLQGDLIESGKPADFALSTDLQVADPATGANINFDGSGKY 115 +G + TGV+ ++ + + QGDL+E+ D AL + Sbjct: 61 VAPVIGELTTGVYLQERMPNFRQGDLVETQNNTDVALL--------------------QG 100 Query: 116 ITPDGEVIYRPQA-FFTVQDNEGNQLYTRNGSFTVNAAGEVLSAGGFKVVDSNGNPLILT 174 + P E +P A F+TVQ+ G YTRNG+FTV+ G + + GF V+ ++GNPL + Sbjct: 101 VMPTNEENGQPWALFYTVQNENGEVRYTRNGNFTVDGDGFLTTGDGFYVLGTDGNPLQV- 159 Query: 175 GSQDNLKVDGQGNLLNPATGLPSGTRLGISVVTKPQELVRDGNGVFHADDVENADIRFAN 234 + + VDG G + + A G G ++ + V P +LV++GNG+ + N Sbjct: 160 -NNEWFNVDGDGMITDEA-GAAIG-QINVVVAEDPMQLVKEGNGLLRFEGDGELPTGVGN 216 Query: 235 GTDNLQVRQGYLENSNVDATKVTVDMNAAYRAYEANQKVVQFYDSSLQKAVNEVGRI 291 N Q++QG++E SNVDA + +M AYR +EANQ+V++ YD SL KAVNEVGRI Sbjct: 217 AAVNYQLQQGFIERSNVDAAQTMTEMMQAYRTFEANQQVLRAYDESLGKAVNEVGRI 273 >B4BP36.1 B4BP36_9BACI Flagellar basal body rod protein Length = 295 Score = 189 bits (479), Expect = 4e-46, Method: Compositional matrix adjust. Identities = 113/302 (37%), Positives = 159/302 (52%), Gaps = 38/302 (12%) Query: 1 MLRGLYTAAAGMVTQQRRHDTATQNIANLNTTGYKQVDSVSHAFPEVLISAMSDGRIKP- 59 MLRGLYTAA+GM+ QQRR D T N+AN T GYK AFPE+L+S + D I Sbjct: 20 MLRGLYTAASGMLAQQRRGDWLTNNLANAETPGYKADAGAMRAFPELLMSRLEDESIPSA 79 Query: 60 ----------VGRMNTGVFAEQSVSQYLQGDLIESGKPADFALSTDLQVADPATGANINF 109 +G +NTGV+ ++ + + QGD+ E+G D AL DP TG Sbjct: 80 PPRAVPTQTAIGLLNTGVYTQELIPNFRQGDIKETGLSTDVALVDGQVPVDPTTGE---- 135 Query: 110 DGSGKYITPDGEVIYRPQAFFTVQDNEGNQLYTRNGSFTVNAAGEVLSAGGFKVVDSNGN 169 R FF V++ +G YTRNG FT++ G + + G+ V+D NG Sbjct: 136 ---------------RGTLFFAVENGQGETRYTRNGHFTLSPDGYLTTQDGWNVLDGNGE 180 Query: 170 PLILTGSQDNLKVDGQGNLLNPATGLPSGTRLGISVVTKPQELVRDGNGVFHADDVENAD 229 + + ++ DG N RLG+ P+ +V++GNG+F + + Sbjct: 181 RIAIPSESFTVRDDGTITAENRTIA-----RLGVVFAANPKTVVKEGNGLFRSTE---GP 232 Query: 230 IRFANGTDNLQVRQGYLENSNVDATKVTVDMNAAYRAYEANQKVVQFYDSSLQKAVNEVG 289 + A G V+QG++E SNVD + D+ AAYRA+EANQK++Q YD SL KAVNEVG Sbjct: 233 LPQAPGNVTYMVKQGFIERSNVDIEQTMTDLLAAYRAFEANQKIIQAYDRSLDKAVNEVG 292 Query: 290 RI 291 R+ Sbjct: 293 RL 294 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.313 0.131 0.366 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,771,314,032 Number of extensions: 80480832 Number of successful extensions: 160761 Number of sequences better than 1.0e-05: 1328 Number of HSP's gapped: 159106 Number of HSP's successfully gapped: 1359 Length of query: 291 Length of database: 3,060,696,203 Length adjustment: 134 Effective length of query: 157 Effective length of database: 1,798,280,193 Effective search space: 282329990301 Effective search space used: 282329990301 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 130 (54.7 bits)