BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0327.0001.p (569 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K7J2.1 B3K7J2_9BACI Stage II sporulation protein D 346 4e-93 B1DEE5.1 B1DEE5_9BACL Stage II sporulation protein D 295 8e-78 C0Z759.1 C0Z759_BREBN Stage II sporulation protein D 277 2e-72 A4ITH3.1 A4ITH3_GEOTN Sporulation protein SpoIID 276 4e-72 B4BP50.1 B4BP50_9BACI Stage II sporulation protein D 276 4e-72 >B3K7J2.1 B3K7J2_9BACI Stage II sporulation protein D Length = 536 Score = 346 bits (888), Expect = 4e-93, Method: Compositional matrix adjust. Identities = 174/303 (57%), Positives = 219/303 (72%), Gaps = 14/303 (4%) Query: 266 QPKVSVYLSHSGQIETLPLEEYVSGVVAAEMPPGFELEALKAQAVAARTFIVRRLQAGDH 325 +P VSVY++ + +E LPLE+Y+ GV+AAEMP F+LEALKAQA+AART+IV+RL +GD Sbjct: 230 EPTVSVYVTKTDTVEELPLEQYLVGVLAAEMPAEFQLEALKAQAIAARTYIVQRLASGDR 289 Query: 326 SGVPVPGADVTDTVSHQAYVSKAALERKWTRGGRSAELAKVRRAVLETRGVVMTYQGQPI 385 SGVP A VTDTVSHQAY+++ L ++W R G+ AEL K+R+AV ET GVVMTY+G+PI Sbjct: 290 SGVPDGKALVTDTVSHQAYIAQDELTKEWRRLGKEAELTKLRQAVKETAGVVMTYEGKPI 349 Query: 386 TASFFASSGGYTENSEDYWNAAVPYLRSVASPWDQDITPNLAVTYTFSNAELIQKLGIXX 445 TASFF++S GYTENSEDYW +PYLRSV SPWD+ I+P T T +E I KLG+ Sbjct: 350 TASFFSTSNGYTENSEDYWKNEIPYLRSVESPWDKQISPKYKTTVTMPRSEFIGKLGLAD 409 Query: 446 XXXXXXXXXXXXGPAKPVSSSSASGLP-AEVLSLTAGHRVQKISIGGMVFTGREVREKLG 504 A PVS+ G +VLS T GHR++ + +GG FTGREVREKLG Sbjct: 410 G-------------AVPVSAGGDGGSSFLKVLSYTQGHRIESVRVGGKKFTGREVREKLG 456 Query: 505 LRSSQFTWKRKGNKVLITTYGNGHGVGMSQWGANGMAKQGSTATQILKHYYSGISFNQVS 564 LRSSQFT K ++ ITTYGNGHGVGMSQ+GA GMA++G A IL+HYY+ ISF++ S Sbjct: 457 LRSSQFTLHMKDGEIEITTYGNGHGVGMSQYGAEGMAREGYEAKAILEHYYTNISFDKTS 516 Query: 565 TLL 567 LL Sbjct: 517 NLL 519 >B1DEE5.1 B1DEE5_9BACL Stage II sporulation protein D Length = 383 Score = 295 bits (755), Expect = 8e-78, Method: Compositional matrix adjust. Identities = 151/305 (49%), Positives = 198/305 (64%), Gaps = 26/305 (8%) Query: 269 VSVYLSHSGQIETLPLEEYVSGVVAAEMPPGFELEALKAQAVAARTFIVRRLQAGDHSGV 328 ++VYL+ + E + LE YV GVVA EMP F LEALKAQA+AART+IVRR++ DHSG+ Sbjct: 99 ITVYLTKEQRTERVTLETYVRGVVAGEMPADFGLEALKAQAIAARTYIVRRIELDDHSGM 158 Query: 329 PVPGA----DVTDTVSHQAYVSKAALERKWTRGGRSAELAKVRRAVLETRGVVMTYQGQP 384 P GA DVTDT+ HQ Y+S L+ W +G + + +AK+ +AV ETRG V+TY+G+P Sbjct: 159 PATGAASRADVTDTIDHQIYISIDKLKELWPKGEQESRMAKLNKAVSETRGEVITYEGEP 218 Query: 385 ITASFFASSGGYTENSEDYWNAAVPYLRSVASPWDQDITPNLAVTYTFSNAELIQKLGIX 444 I A FF++S GYTENSE Y+ +PYLRSVASPWD+ ++P T ++ +LG+ Sbjct: 219 IQAVFFSTSNGYTENSEQYFQEELPYLRSVASPWDKQLSPRYQETVKLQLSDFNARLGLA 278 Query: 445 XXXXXXXXXXXXXGPAKPVSSSSASGLPAEVLSLTAGHRVQKISIGGMVFTGREVREKLG 504 KPV VL T G+R+ I I G +TGREVREKLG Sbjct: 279 SKK-------------KPV---------IRVLDKTEGNRIADILIDGKTYTGREVREKLG 316 Query: 505 LRSSQFTWKRKGNKVLITTYGNGHGVGMSQWGANGMAKQGSTATQILKHYYSGISFNQVS 564 L SS+FTWK G+++ ITT+G GHGVGMSQWGANGMA++G +A QI+ +YY+G Q S Sbjct: 317 LASSEFTWKLDGDEIDITTFGYGHGVGMSQWGANGMAEEGKSAEQIVTYYYTGTKVEQAS 376 Query: 565 TLLKK 569 L K Sbjct: 377 KLAAK 381 >C0Z759.1 C0Z759_BREBN Stage II sporulation protein D Length = 338 Score = 277 bits (709), Expect = 2e-72, Method: Compositional matrix adjust. Identities = 150/296 (50%), Positives = 188/296 (63%), Gaps = 24/296 (8%) Query: 269 VSVYLSHSGQIETLPLEEYVSGVVAAEMPPGFELEALKAQAVAARTFIVRRLQAGDHSGV 328 V VY + +ETLPLE Y++GVVAAEMP FELEALKAQA+AART+IVRRL+ G V Sbjct: 58 VKVYRTEKKAVETLPLETYITGVVAAEMPAEFELEALKAQALAARTYIVRRLKEGKFDDV 117 Query: 329 PVPGADVTDTVSHQAYVSKAALERKWTRGGRSAELA--KVRRAVLETRGVVMTYQGQPIT 386 P G V DTV HQ Y+ + +W G E ++++AV+ T GV++TYQ +PI Sbjct: 118 P-SGGQVLDTVQHQVYMDEKQRRERW---GDQYEWKNKRIQQAVMATAGVILTYQNEPID 173 Query: 387 ASFFASSGGYTENSEDYWNAAVPYLRSVASPWDQDITPNLAVTYTFSNAELIQKLGIXXX 446 A+FF++S G+TENS++YW +PYL+SVASPWD +P T S EL + LG+ Sbjct: 174 ATFFSTSNGFTENSDEYWEKPIPYLKSVASPWDIQ-SPRYEETVVLSTVELEKNLGV--- 229 Query: 447 XXXXXXXXXXXGPAKPVSSSSASGLPAEVLSLTAGHRVQKISIGGMVFTGREVREKLGLR 506 K +S SG + S T G+RV ISIGG FTGRE REKL L Sbjct: 230 --------------KLTQEASTSGSWYRIESRTTGNRVGTISIGGKEFTGREFREKLNLN 275 Query: 507 SSQFTWKRKGNKVLITTYGNGHGVGMSQWGANGMAKQGSTATQILKHYYSGISFNQ 562 SS FT +GN+V ITT G GHGVGMSQWGANGMAK G +A QI+KH+Y GIS + Sbjct: 276 SSSFTLDLRGNQVFITTKGYGHGVGMSQWGANGMAKSGKSAEQIVKHFYQGISLQE 331 >A4ITH3.1 A4ITH3_GEOTN Sporulation protein SpoIID Length = 342 Score = 276 bits (706), Expect = 4e-72, Method: Compositional matrix adjust. Identities = 146/301 (48%), Positives = 196/301 (65%), Gaps = 24/301 (7%) Query: 269 VSVYLSHSGQIETLPLEEYVSGVVAAEMPPGFELEALKAQAVAARTFIVRRLQAGDHSGV 328 V+VY S ++E +PLE+YV GVVAAEMP FELEALKAQA+ ART+IV++L A + Sbjct: 61 VAVYRSREQRVEKIPLEQYVIGVVAAEMPAEFELEALKAQALTARTYIVKQLLANQPFRL 120 Query: 329 PVPGADVTDTVSHQAYVSKAALERKWTRGGRSAELAKVRRAVLETRGVVMTYQGQPITAS 388 P GA+VTDTV+HQ Y S A L + W ++ K+ +AVL+TRG ++TY +PI A Sbjct: 121 P-EGANVTDTVTHQVYYSDAELRKLWG-SDYDWKIKKITKAVLDTRGQILTYNNEPIEAL 178 Query: 389 FFASSGGYTENSEDYWNAAVPYLRSVASPWDQDITPNLAVTYTFSNAELIQKLGIXXXXX 448 FF++S GYTENSE YW PYL+SVASPWD+ +P T + +E ++LG+ Sbjct: 179 FFSTSNGYTENSEAYWQNDFPYLKSVASPWDEQ-SPKFYQRKTMAVSEFERRLGVT---- 233 Query: 449 XXXXXXXXXGPAKPVSSSSASGLPAEVLSLTAGHRVQKISIGGMVFTGREVREKLGLRSS 508 +SS + G+ ++S T G RV ++ IGG FTGREVRE+LGL S+ Sbjct: 234 --------------LSSDGSVGV---IVSRTPGRRVGEVKIGGKTFTGREVRERLGLPST 276 Query: 509 QFTWKRKGNKVLITTYGNGHGVGMSQWGANGMAKQGSTATQILKHYYSGISFNQVSTLLK 568 FTW RKG++++ITT G GHGVGMSQ+GAN MAK+G T I+KHYY G+ + + L Sbjct: 277 DFTWARKGDEIIITTKGYGHGVGMSQYGANFMAKEGKTYADIVKHYYRGVRISSATAFLN 336 Query: 569 K 569 K Sbjct: 337 K 337 >B4BP50.1 B4BP50_9BACI Stage II sporulation protein D Length = 342 Score = 276 bits (706), Expect = 4e-72, Method: Compositional matrix adjust. Identities = 146/301 (48%), Positives = 196/301 (65%), Gaps = 24/301 (7%) Query: 269 VSVYLSHSGQIETLPLEEYVSGVVAAEMPPGFELEALKAQAVAARTFIVRRLQAGDHSGV 328 V+VY S ++E +PLE+YV GVVAAEMP FELEALKAQA+ ART+IV++L A + Sbjct: 61 VAVYRSREQRVEKIPLEQYVIGVVAAEMPAEFELEALKAQALTARTYIVKQLLANQPFRL 120 Query: 329 PVPGADVTDTVSHQAYVSKAALERKWTRGGRSAELAKVRRAVLETRGVVMTYQGQPITAS 388 P GA+VTDTV+HQ Y S A L + W ++ K+ +AVL+TRG ++TY +PI A Sbjct: 121 P-EGANVTDTVTHQVYYSDAELRKLWG-SDYDWKIKKITKAVLDTRGQILTYNNEPIEAL 178 Query: 389 FFASSGGYTENSEDYWNAAVPYLRSVASPWDQDITPNLAVTYTFSNAELIQKLGIXXXXX 448 FF++S GYTENSE YW PYL+SVASPWD+ +P T + +E ++LG+ Sbjct: 179 FFSTSNGYTENSEAYWQNDFPYLKSVASPWDEQ-SPKFYQRKTMAVSEFERRLGVT---- 233 Query: 449 XXXXXXXXXGPAKPVSSSSASGLPAEVLSLTAGHRVQKISIGGMVFTGREVREKLGLRSS 508 +SS + G+ ++S T G RV ++ IGG FTGREVRE+LGL S+ Sbjct: 234 --------------LSSDGSVGV---IVSRTPGRRVGEVKIGGKTFTGREVRERLGLPST 276 Query: 509 QFTWKRKGNKVLITTYGNGHGVGMSQWGANGMAKQGSTATQILKHYYSGISFNQVSTLLK 568 FTW RKG++++ITT G GHGVGMSQ+GAN MAK+G T I+KHYY G+ + + L Sbjct: 277 DFTWARKGDEIIITTKGYGHGVGMSQYGANFMAKEGKTYADIVKHYYRGVRISSATAFLN 336 Query: 569 K 569 K Sbjct: 337 K 337 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.315 0.129 0.382 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,735,236,687 Number of extensions: 105355542 Number of successful extensions: 263703 Number of sequences better than 1.0e-05: 455 Number of HSP's gapped: 262909 Number of HSP's successfully gapped: 527 Length of query: 569 Length of database: 3,060,696,203 Length adjustment: 141 Effective length of query: 428 Effective length of database: 1,732,333,088 Effective search space: 741438561664 Effective search space used: 741438561664 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 134 (56.2 bits)