BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0320.0012.p (427 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1DHG3.1 B1DHG3_9BACL Histidyl-tRNA synthetase EC=6.1.1.21 494 e-138 B3KCX5.1 B3KCX5_9BACI Histidyl-tRNA synthetase EC=6.1.1.21 484 e-135 C3F453.1 C3F453_BACTU Histidyl-tRNA synthetase 1 335 4e-90 C2QEB6.1 C2QEB6_BACCE Histidyl-tRNA synthetase 1 335 4e-90 C2X0W5.1 C2X0W5_BACCE Histidyl-tRNA synthetase 1 333 1e-89 >B1DHG3.1 B1DHG3_9BACL Histidyl-tRNA synthetase EC=6.1.1.21 Length = 427 Score = 494 bits (1272), Expect = e-138, Method: Compositional matrix adjust. Identities = 246/422 (58%), Positives = 301/422 (71%), Gaps = 3/422 (0%) Query: 1 MQNVKGTYDYFGREQAIRQKVRSTLSELFELYDFESMDXXXXXXXXXXXSKYAGGDEILR 60 MQNVKGTYD+FG+EQA+RQKVRS L E+FELYD+ESM+ SKYAGGDEIL+ Sbjct: 1 MQNVKGTYDFFGKEQAVRQKVRSVLQEVFELYDYESMESTVLHELELLTSKYAGGDEILK 60 Query: 61 EMYQLTDQGGRQLGLRYDLTIPFAKVIALNPGIEFPYKRYEIGKVFRDGPVKKGRLREFL 120 EMY+ TDQG R+LGLRYDLTIP AKV+ALNPGI+ PY+RYEIGKVFRDGPVK+GRLREFL Sbjct: 61 EMYRFTDQGARKLGLRYDLTIPLAKVMALNPGIKLPYRRYEIGKVFRDGPVKRGRLREFL 120 Query: 121 QCDADVVGIAGPQGEAELMQLAAEAFRRLEIAVTLKWNNRRFLGEILTAVGVPAXXXXEH 180 QCD D+VG+AGP+ EAELM +A E F+RL+I VTL+WNNRRFL EIL AVGVPA + Sbjct: 121 QCDVDMVGVAGPEAEAELMLIAVEVFKRLDIPVTLRWNNRRFLSEILDAVGVPA---EDS 177 Query: 181 LSVMLTLDKLAKIGSRGVLAELEGKGVAPAAVSALAELLDMDAPSFEELAENYGLGGEPG 240 LSVMLTLDKL KIGS GVL E KG++ V +AEL+ M PSF LA+ YGL G Sbjct: 178 LSVMLTLDKLEKIGSDGVLKEWRDKGISGPTVDEIAELIAMSGPSFSLLADRYGLKESRG 237 Query: 241 ALEVLALQRLIAAVGLAETCVFDPFLSRGLSFYTGTVYEIFDASGAYTSSLGGGGRYDSI 300 A E+ ALQ LI + L++ C FDPFLSRGLSFYTGTVYEIFDA G Y SS+G GGRYD+I Sbjct: 238 AAEIRALQTLIDKLSLSDICRFDPFLSRGLSFYTGTVYEIFDAGGLYASSIGSGGRYDAI 297 Query: 301 IGKLVGREDIEYPTVGISFGMESIMALLGERAVRVETSGVLVIPVGECMPQXXXXXXXXX 360 IGKL+G ED+ YP VG+SFGMESIM ++ +R + T V+V+ +G+ M + Sbjct: 298 IGKLIGSEDMAYPAVGLSFGMESIMEMIRDRPIPAPTPKVVVLSIGDTMGEALMAATALR 357 Query: 361 XXXXXXXVDXXXXXXXXXXXXXXXXXIRYVILVGESEAAEGKLRLKDMSQQSEELLTVEE 420 ++ IRY +L+GE E G++RLKDM + E + ++E Sbjct: 358 SNGIQTGMEPAGRKLKKALASLESRSIRYALLIGEEEVRTGQVRLKDMKAREEYAVPLDE 417 Query: 421 AI 422 A+ Sbjct: 418 AL 419 >B3KCX5.1 B3KCX5_9BACI Histidyl-tRNA synthetase EC=6.1.1.21 Length = 428 Score = 484 bits (1247), Expect = e-135, Method: Compositional matrix adjust. Identities = 250/427 (58%), Positives = 295/427 (69%), Gaps = 3/427 (0%) Query: 1 MQNVKGTYDYFGREQAIRQKVRSTLSELFELYDFESMDXXXXXXXXXXXSKYAGGDEILR 60 MQNVKGTYDYFG+EQA+R+KV STL E+FE YD+ M+ SKYAGG+EIL+ Sbjct: 1 MQNVKGTYDYFGQEQAVRRKVLSTLREVFECYDYAEMESTILNELELLSSKYAGGEEILK 60 Query: 61 EMYQLTDQGGRQLGLRYDLTIPFAKVIALNPGIEFPYKRYEIGKVFRDGPVKKGRLREFL 120 EMYQLTDQG RQLGLRYDLTIPFAKVIALNPGI P+KRYE+GKVFRDGPVK+GRLREFL Sbjct: 61 EMYQLTDQGARQLGLRYDLTIPFAKVIALNPGISQPFKRYEMGKVFRDGPVKRGRLREFL 120 Query: 121 QCDADVVGIAGPQGEAELMQLAAEAFRRLEIAVTLKWNNRRFLGEILTAVGVPAXXXXEH 180 QCD DVVG+AGP+ EAELMQLA E FRRLEI + L+WNNR FLGE+L +GVPA Sbjct: 121 QCDVDVVGVAGPEAEAELMQLAVEVFRRLEIPIVLRWNNRGFLGELLGGMGVPADLSS-- 178 Query: 181 LSVMLTLDKLAKIGSRGVLAELEGKGVAPAAVSALAELLDMDAPSFEELAENYGLGGEPG 240 SVMLTLDKL KIG GV EL K + P + +D+ P+FE++ Y L G Sbjct: 179 -SVMLTLDKLDKIGREGVRKELADKVIDPEICRRVLSFVDLKEPTFEQITRTYNLEPGRG 237 Query: 241 ALEVLALQRLIAAVGLAETCVFDPFLSRGLSFYTGTVYEIFDASGAYTSSLGGGGRYDSI 300 A EV LQ L+ VGL + CVFDPFLSRGLSFYTGTVYEI+DAS A+ SSLGGGGRYD+I Sbjct: 238 AREVQILQSLLQRVGLQDICVFDPFLSRGLSFYTGTVYEIYDASHAFRSSLGGGGRYDAI 297 Query: 301 IGKLVGREDIEYPTVGISFGMESIMALLGERAVRVETSGVLVIPVGECMPQXXXXXXXXX 360 IG+LVGRED+ YPTVG+SFGMESIM +L +R V V+VIP+G+ +P+ Sbjct: 298 IGQLVGREDMAYPTVGLSFGMESIMEMLRDRVVETNRPHVVVIPIGDAVPEGLMAAAALR 357 Query: 361 XXXXXXXVDXXXXXXXXXXXXXXXXXIRYVILVGESEAAEGKLRLKDMSQQSEELLTVEE 420 + I Y IL+GE EAA G LRLK+MS E L + E Sbjct: 358 NRGIRTRLATLRRKLKKTLASVSAEGIPYAILIGEDEAAAGVLRLKNMSDGIELTLKLNE 417 Query: 421 AIAKLAR 427 A ++R Sbjct: 418 AEEFISR 424 >C3F453.1 C3F453_BACTU Histidyl-tRNA synthetase 1 Length = 428 Score = 335 bits (860), Expect = 4e-90, Method: Compositional matrix adjust. Identities = 175/417 (41%), Positives = 244/417 (58%), Gaps = 10/417 (2%) Query: 1 MQNVKGTYDYFGREQAIRQKVRSTLSELFELYDFESMDXXXXXXXXXXXSKYAGGDEILR 60 M+NVKGT DY EQ +R K++ + FE Y +S++ KY GGDEIL+ Sbjct: 6 MRNVKGTKDYLPEEQVLRNKIKRACEDTFERYGCKSLETPTLNMYELMSYKYGGGDEILK 65 Query: 61 EMYQLTDQGGRQLGLRYDLTIPFAKVIALNPGIEFPYKRYEIGKVFRDGPVKKGRLREFL 120 E+Y L DQG R L LRYDLTIPFAKV+A+NP I P+KRYEIGKVFRDGP+K+GR REF+ Sbjct: 66 EIYTLQDQGKRALALRYDLTIPFAKVVAMNPNIRLPFKRYEIGKVFRDGPIKQGRFREFI 125 Query: 121 QCDADVVGIAGPQGEAELMQLAAEAFRRLEIAVTLKWNNRRFLGEILTAVGVPAXXXXEH 180 QCD D+VG+ EAELM +A E FR L + VT+++NNR+ L IL A+ +P + Sbjct: 126 QCDVDIVGVESVMAEAELMSMAFELFRTLNLEVTIQYNNRKLLNGILQAINIPTELTSD- 184 Query: 181 LSVMLTLDKLAKIGSRGVLAELEGKGV----APAAVSALAELLDMDAPSFEELAENYGLG 236 V+L+LDK+ KIG GV ++ +G+ A + + L + F+E N + Sbjct: 185 --VILSLDKIEKIGINGVRKDVLERGISEEMADTICNTILSCLQLSMADFKEAFNNPLVA 242 Query: 237 GEPGALEVLALQRLIAAVGLAETCVFDPFLSRGLSFYTGTVYEIFDASGAYTSSLGGGGR 296 G E+ LQ+ I A+G+ E +F+PFL+RGL+ YTGTVYEIF G+ TSS+G GGR Sbjct: 243 D--GVNELQQLQQFIIALGINENTIFNPFLARGLTMYTGTVYEIFLKDGSITSSIGSGGR 300 Query: 297 YDSIIGKLVGREDIEYPTVGISFGMESIMALLGERAVRVETSGVLVIPVGECMPQXXXXX 356 YD+IIG G +D+ YPTVGISFG++ I L ++ T+ V +IP+G + Sbjct: 301 YDNIIGAFRG-DDMSYPTVGISFGLDVIYTALSQKETISSTADVFIIPLGTELQCLQIAQ 359 Query: 357 XXXXXXXXXXXVDXXXXXXXXXXXXXXXXXIRYVILVGESEAAEGKLRLKDMSQQSE 413 ++ I YV+++GE E + L++M + SE Sbjct: 360 QLRSTTSLKIELELAGRKLKRALNYANKENIPYVLIIGEEEICTETVMLRNMKEGSE 416 >C2QEB6.1 C2QEB6_BACCE Histidyl-tRNA synthetase 1 Length = 436 Score = 335 bits (860), Expect = 4e-90, Method: Compositional matrix adjust. Identities = 172/417 (41%), Positives = 247/417 (59%), Gaps = 10/417 (2%) Query: 1 MQNVKGTYDYFGREQAIRQKVRSTLSELFELYDFESMDXXXXXXXXXXXSKYAGGDEILR 60 M+NVKGT DY EQ +R K++ + FE Y + ++ KY GGDEIL+ Sbjct: 3 MKNVKGTKDYLPEEQVLRNKIKRACEDTFEQYGCKPLETPTLNMYELMSYKYGGGDEILK 62 Query: 61 EMYQLTDQGGRQLGLRYDLTIPFAKVIALNPGIEFPYKRYEIGKVFRDGPVKKGRLREFL 120 E+Y L DQG R+L LRYDLTIPFAKV+A+NP I P+KRYEIGKVFRDGP+K+GR REF+ Sbjct: 63 EIYTLQDQGKRELALRYDLTIPFAKVVAMNPNIRLPFKRYEIGKVFRDGPIKQGRFREFI 122 Query: 121 QCDADVVGIAGPQGEAELMQLAAEAFRRLEIAVTLKWNNRRFLGEILTAVGVPAXXXXEH 180 QCD D+VG+ EAELM +A E FR L + VT+++NNR+ L IL A+ +P + Sbjct: 123 QCDVDIVGVESVMAEAELMSMAFELFRTLNLEVTIQYNNRKLLNGILQAINIPTERTSD- 181 Query: 181 LSVMLTLDKLAKIGSRGVLAELEGKGV----APAAVSALAELLDMDAPSFEELAENYGLG 236 V+L+LDK+ KIG GV ++ +G+ A + + L + F+E N + Sbjct: 182 --VILSLDKIEKIGIDGVRKDVLERGISEEMADTICNTVLSCLKLTIADFKEAFNNQLVT 239 Query: 237 GEPGALEVLALQRLIAAVGLAETCVFDPFLSRGLSFYTGTVYEIFDASGAYTSSLGGGGR 296 G E+ LQ+ + A+G++E +F+PFL+RGL+ YTGTVYEIF G+ TSS+G GGR Sbjct: 240 D--GVNELQQLQQYLIALGISENAIFNPFLARGLTMYTGTVYEIFLKDGSITSSIGSGGR 297 Query: 297 YDSIIGKLVGREDIEYPTVGISFGMESIMALLGERAVRVETSGVLVIPVGECMPQXXXXX 356 YD+IIG G +++ YPTVGISFG++ I L ++ T+ + +IP+G +P Sbjct: 298 YDNIIGAFRG-DNMNYPTVGISFGLDVIYTALSQKETISSTADLFIIPLGTELPCLQIAQ 356 Query: 357 XXXXXXXXXXXVDXXXXXXXXXXXXXXXXXIRYVILVGESEAAEGKLRLKDMSQQSE 413 ++ I YV+++GE E + + L++M + SE Sbjct: 357 QLRSTTSLKVELELAGRKLKRALNYANKENIPYVLIIGEEELSTNTVMLRNMKEGSE 413 >C2X0W5.1 C2X0W5_BACCE Histidyl-tRNA synthetase 1 Length = 428 Score = 333 bits (855), Expect = 1e-89, Method: Compositional matrix adjust. Identities = 172/417 (41%), Positives = 247/417 (59%), Gaps = 10/417 (2%) Query: 1 MQNVKGTYDYFGREQAIRQKVRSTLSELFELYDFESMDXXXXXXXXXXXSKYAGGDEILR 60 M+NVKGT DY EQ +R K++ + FE Y + ++ KY GGDEIL+ Sbjct: 6 MKNVKGTKDYLPEEQVLRNKIKRACEDTFERYGCKPLETPTLNMYELMSYKYGGGDEILK 65 Query: 61 EMYQLTDQGGRQLGLRYDLTIPFAKVIALNPGIEFPYKRYEIGKVFRDGPVKKGRLREFL 120 E+Y L DQG R+L LRYDLTIPFAKV+A+NP I P+KRYEIGKVFRDGP+K+GR REF+ Sbjct: 66 EIYTLQDQGKRELALRYDLTIPFAKVVAMNPNIRLPFKRYEIGKVFRDGPIKQGRFREFI 125 Query: 121 QCDADVVGIAGPQGEAELMQLAAEAFRRLEIAVTLKWNNRRFLGEILTAVGVPAXXXXEH 180 QCD D+VG+ EAELM +A E FR L + VT+++NNR+ L IL A+ +P + Sbjct: 126 QCDVDIVGVESVMAEAELMSMAFELFRTLNLEVTIQYNNRKLLNGILQAINIPTELMSD- 184 Query: 181 LSVMLTLDKLAKIGSRGVLAELEGKGV----APAAVSALAELLDMDAPSFEELAENYGLG 236 V+L+LDK+ KIG GV ++ G+G+ A + + L + F+E N + Sbjct: 185 --VILSLDKIEKIGIDGVRKDILGRGITEEMADTICNTVLSCLKLTIADFKEAFNNQLVT 242 Query: 237 GEPGALEVLALQRLIAAVGLAETCVFDPFLSRGLSFYTGTVYEIFDASGAYTSSLGGGGR 296 G E+ LQ+ + A+G+ E +F+PFL+RGL+ YTGTVYEIF G+ TSS+G GGR Sbjct: 243 D--GINELQQLQQYLIALGINENAIFNPFLARGLTMYTGTVYEIFLKDGSITSSIGSGGR 300 Query: 297 YDSIIGKLVGREDIEYPTVGISFGMESIMALLGERAVRVETSGVLVIPVGECMPQXXXXX 356 YD+IIG G +++ YPTVGISFG++ I L ++ + T+ + +IP+G + Sbjct: 301 YDNIIGAFRG-DNMNYPTVGISFGLDVIYTALSQKETKSSTADLFIIPLGTELQCLQIAQ 359 Query: 357 XXXXXXXXXXXVDXXXXXXXXXXXXXXXXXIRYVILVGESEAAEGKLRLKDMSQQSE 413 ++ I YV+++GE E + + L++M + SE Sbjct: 360 QLRSTTSLKIELELAGRKLKRALNYANKENIPYVLIIGEEELSAETVMLRNMKEGSE 416 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.139 0.389 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,161,369,663 Number of extensions: 85937385 Number of successful extensions: 211234 Number of sequences better than 1.0e-05: 1344 Number of HSP's gapped: 208480 Number of HSP's successfully gapped: 1364 Length of query: 427 Length of database: 3,060,696,203 Length adjustment: 139 Effective length of query: 288 Effective length of database: 1,751,175,118 Effective search space: 504338433984 Effective search space used: 504338433984 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 133 (55.8 bits)