BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0315.0008.p (415 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1DI84.1 B1DI84_9BACL Integral membrane sensor signal transducti... 374 e-101 A3IDF5.1 A3IDF5_9BACI Two-component sensor histidine kinase 246 3e-63 Q5WJZ1.1 Q5WJZ1_BACSK Two-component sensor histidine kinase 244 1e-62 C5DAH0.1 C5DAH0_9BACI Histidine kinase 234 1e-59 C2VA57.1 C2VA57_BACCE Sensor histidine kinase 216 4e-54 >B1DI84.1 B1DI84_9BACL Integral membrane sensor signal transduction histidine kinase Length = 435 Score = 374 bits (959), Expect = e-101, Method: Compositional matrix adjust. Identities = 196/405 (48%), Positives = 251/405 (61%), Gaps = 2/405 (0%) Query: 10 GEFKINPFDGDIFRIXXXXXXXXXXXXXXXXXPYVITGVATGIVVLLFRTAMD-AVWXXX 68 GEFKINPF G++FRI PY TG+A G VLLFR D A Sbjct: 30 GEFKINPFSGEVFRIGLGSSAFLFLLLLMSHLPYWRTGIAAGATVLLFRVTFDGAASPGT 89 Query: 69 XXXXXXXXXHFSAMVYYIVFALLMNLIKSRIDSFHPLVLGGVAALIDLLSNEMELLTRLI 128 HFSAM+YYIVFA M LI+ R+ +PL+LG +LID +SNE ELL R + Sbjct: 90 FYLLGSLKTHFSAMLYYIVFAAGMALIQRRLHQMNPLILGACVSLIDFVSNEAELLMRSV 149 Query: 129 VLDSATFRLNEWTYLMAIAVLRTYFTTGVYSSISVSQLRITQREQTRRIEQMLGFGSGLY 188 F+ N+W + IAV+R+YFTTG+YSSI+V Q+R EQ +RIEQM+ GSGLY Sbjct: 150 AFGLPYFQTNQWMLIPVIAVVRSYFTTGIYSSIAVHQMRSVHAEQQKRIEQMINVGSGLY 209 Query: 189 GEVFYLEKSIGTLENVTLSSYELYRNLKAEEEMPRYSRQVLEITQQIHEVKKDSQRILAG 248 GEVFYL KS+ +E +T S++LY L +E + YSR VLEITQQIHEVKKDSQRILAG Sbjct: 210 GEVFYLRKSMDVIERITAKSHDLY-GLLNQEGLNGYSRSVLEITQQIHEVKKDSQRILAG 268 Query: 249 LVKLAEREVTGDMPISDILRFTVKSNANYAEMLGKQISFSIQHTSDYTTDSYIPXXXXXX 308 L+KLA+ E +M +++I+ +KSN Y+ ML K++ +DY T YIP Sbjct: 269 LMKLADLESAPEMTLAEIVELVIKSNGEYSRMLKKEVRIEKDVGNDYLTAHYIPLITVLN 328 Query: 309 XXXXXXVEVIQGKGSISLDMYEKEDNTLFTVTDSGSGIPERDLELVFEPGFTTKFNEEGV 368 VE I+ G+I + + E+E TLF V+D+G+GI E+D ++VFEPGFTTKFN+EGV Sbjct: 329 NLAANSVEAIERHGTIRIRVSEREGETLFIVSDTGTGIAEQDQDMVFEPGFTTKFNQEGV 388 Query: 369 AATGIGLSHVRDIVHLFAGEITVQPASHSGGAMFQIIVPTAQLRK 413 AATGIGLSHVRDIV F G + F PT +L+K Sbjct: 389 AATGIGLSHVRDIVGSFTGRFLLMEHGREAETTFIAAFPTVKLKK 433 >A3IDF5.1 A3IDF5_9BACI Two-component sensor histidine kinase Length = 403 Score = 246 bits (628), Expect = 3e-63, Method: Compositional matrix adjust. Identities = 153/415 (36%), Positives = 226/415 (54%), Gaps = 13/415 (3%) Query: 1 MAAAGTAVAGEFKINPFDGDIFRIXXXXXXXXXXXXXXXXXPYVITGVATGIVVLLFRTA 60 M A TA+ E KI PF FR P ++ G AT IV ++FR Sbjct: 1 MIAVLTAIGSEIKIMPFANTTFRFGLGTIIFFLCTLIKPT-PIILAGFATSIVTIIFRVF 59 Query: 61 MDAVWXXXXXXXXXXXXHFSAMVYYIVFALLMNLIKSRIDSFHPLVLGGVAALIDLLSNE 120 + H A ++Y++FA+ + ++ + PL LG + A +++SN Sbjct: 60 NS--YLHDISVLESIFNHLPAGLFYVLFAICLQILDVQTYREKPLKLGLLVAFSEVISNL 117 Query: 121 MELLTRLIVLDSATFRLNEWTYLMAIAVLRTYFTTGVYSSISVSQLRITQREQTRRIEQM 180 E + R V L++ L+A+A+LR++F G+YSSI ++ EQ +R+++M Sbjct: 118 AEQIFRFFVQTYTFLFLHDLLILLAVALLRSFFVVGIYSSILIA-------EQKKRVQEM 170 Query: 181 LGFGSGLYGEVFYLEKSIGTLENVTLSSYELYRNLKAEEEMPRYSRQVLEITQQIHEVKK 240 L GS LY E YL+KS+ +E +T S +ELYR LKA+ S Q L I Q+IHEVKK Sbjct: 171 LNIGSDLYVETLYLKKSMNHIETITASGFELYRQLKAQGYRAE-SLQALHIAQEIHEVKK 229 Query: 241 DSQRILAGLVKLAEREVTGDMPISDILRFTVKSNANYAEMLGKQISFSIQHTSDYTTDSY 300 DSQRI AG+ K+ G +S++L + N+ YAE+LGK I F D+ T + Sbjct: 230 DSQRIYAGISKIVGERSFGPFILSELLHYIEDGNSKYAELLGKNIQFETSIDFDFQTKEH 289 Query: 301 IPXXXXXXXXXXXXVEVIQGKGSISLDMYEKEDNTLFTVTDSGSGIPERDLELVFEPGFT 360 I VE I+ +G IS+ + +EDNT+ T+ D+G GI + D ++FEPGFT Sbjct: 290 IALLALLNNLTANAVEAIEEQGIISIAIQRQEDNTVVTICDNGKGISQNDFSIIFEPGFT 349 Query: 361 TKFNEEGVAATGIGLSHVRDIVHLFAGEITVQPASHSGGAMFQIIVPTAQLRKEE 415 TK+N EGVAATGIGLSHV +++ G ITV+ S++ F+II+PT ++ E Sbjct: 350 TKYNVEGVAATGIGLSHVAELIEKLKGSITVE--SNNQYTTFKIIIPTQTIQTGE 402 >Q5WJZ1.1 Q5WJZ1_BACSK Two-component sensor histidine kinase Length = 422 Score = 244 bits (623), Expect = 1e-62, Method: Compositional matrix adjust. Identities = 144/411 (35%), Positives = 221/411 (53%), Gaps = 8/411 (1%) Query: 3 AAGTAVAGEFKINPFDGDIFRIXXXXXXXXXXXXXXXXXPYVITGVATGIVVLLFRTAMD 62 TA+ GE + PF + FR P +TG+ TG VV+LFR Sbjct: 13 GVATALMGELRFAPFGWE-FRFGMGSSTFFFLLLAFRQAPPFLTGILTGAVVVLFRVLTS 71 Query: 63 AVWXXX-XXXXXXXXXHFSAMVYYIVFALLMNLIKSRIDSFHPLVLGGVAALIDLLSNEM 121 ++ H SAM YY F L + L+ +++ + L G +ID+ SN Sbjct: 72 TLFLEDPYSFVEVLFVHLSAMFYYWAFGLGITLLPTKLVQKNMLWFGLFVVVIDIASNLT 131 Query: 122 ELLTRLIVLDSATFRLNEWTYLMAIAVLRTYFTTGVYSSISVSQLRITQREQTRRIEQML 181 EL R ++ D T +W L+ + V+R YF GVY+SI+ + + +EQ +R E +L Sbjct: 132 ELFARSVLQDITTLSWTDWLALLVVTVIRVYFVLGVYASIAQHKQQTILKEQKKRYETLL 191 Query: 182 GFGSGLYGEVFYLEKSIGTLENVTLSSYELYRNLKAEEEMPRYSRQVLEITQQIHEVKKD 241 + L+GE +YL K I T+EN+T ++ LYR LK +E Y ++ L+I+Q+IHE+KKD Sbjct: 192 NVSASLFGESYYLSKVIETVENMTNRAFYLYRKLK--QEQSAYHQEALQISQEIHEIKKD 249 Query: 242 SQRILAGLVKLAEREVTGD-MPISDILRFTVKSNANYAEMLGKQISFSIQHTSDYTTDSY 300 +QRI AGL KL + T + + + + F +K N +Y+ L K+I F + TS Y T+ + Sbjct: 250 AQRIQAGLAKLNNHDHTFEQLNLKQLGDFAIKGNESYSFFLNKRIRFRQEITSTYLTNKH 309 Query: 301 IPXXXXXXXXXXXXVEVIQGKGSISLDMYEKEDNTLFTVTDSGSGIPERDLELVFEPGFT 360 + +E I +G+I L +YEK + T F VTD+G+GI E D+ + EPGFT Sbjct: 310 VELLSCLNNLLANAIESIDKEGTILLSIYEKNEMTYFEVTDNGAGIAEEDMHHLCEPGFT 369 Query: 361 TKFNEEGVAATGIGLSHVRDIVHLFAGEITVQPASHSGGAMFQIIVPTAQL 411 TK+ E+G +TGIGL+HV+ IV G + + SH +++PT +L Sbjct: 370 TKYREDGSPSTGIGLTHVQHIVTDLGGFLQFE-RSHVTKVC--LVIPTKRL 417 >C5DAH0.1 C5DAH0_9BACI Histidine kinase Length = 422 Score = 234 bits (596), Expect = 1e-59, Method: Compositional matrix adjust. Identities = 144/413 (34%), Positives = 219/413 (53%), Gaps = 7/413 (1%) Query: 5 GTAVAGEFKINPFDGDIFRIXXXXXXXXXXXXXXXXXPYVITGVATGIVVLLFRTAMDAV 64 TA GE KINPF+ FR + G+ G V+ FR A+D + Sbjct: 14 ATAFFGEMKINPFNSP-FRFSLGSAIFFFGLISFEMISPLFIGICAGCFVVGFRIALD-M 71 Query: 65 WXXXXXXXXXXXXHFSAMVYYIVFALLMNLIKSRIDSFHPLVLGGVAALIDLLSNEMELL 124 H A YYI FAL++ + + R P+ G + A++D SN ELL Sbjct: 72 LTGQAALSDSFFTHAPAACYYINFALIVQITRFRRLLEFPIRAGLLGAVLDFSSNSCELL 131 Query: 125 TRLIVLDSATFRLNEWTYLMAIAVLRTYFTTGVYSSISVSQLRITQREQTRRIEQMLGFG 184 R ++ +S ++ A+LR++ G+Y+ + LR + + +E+++ Sbjct: 132 FRYLLGESFIVTYQVMMVMLLFAILRSFCVIGLYNIFVMKHLRTLGEARQQELERLMMIN 191 Query: 185 SGLYGEVFYLEKSIGTLENVTLSSYELYRNLKAEEEMPRYSRQVLEITQQIHEVKKDSQR 244 +GLY E FYL+KS+ LE +T SYELY L E++ ++ L I + IHEVKKD QR Sbjct: 192 TGLYEETFYLQKSMTYLEEITRKSYELYSRLMEGEKVKPHA--ALYIAEHIHEVKKDVQR 249 Query: 245 ILAGLVKL-AEREVTGDMPISDILRFTVKSNANYAEMLGKQISFSIQHTSDYTTDSYIPX 303 I AGL KL ++ + +PI+++ V++N YAE+LGK+I F+ D +TD Sbjct: 250 IFAGLSKLIGQQPLRRRLPINELCGMVVRANEKYAELLGKKIQFAQTCHVDLSTDHVYAL 309 Query: 304 XXXXXXXXXXXVEVIQGKGSISLDMYEKEDNTLFTVTDSGSGIPERDLELVFEPGFTTKF 363 VE I GSI L + ++ N + VTDSG GI + D + +F+PGFTTK+ Sbjct: 310 LSVLNNLVANAVEAIPTSGSIHLQVELQQSNLIVEVTDSGIGIAKEDADWIFQPGFTTKY 369 Query: 364 NEEGVAATGIGLSHVRDIVHLFAGEITVQPASHSGGAMFQIIVPTAQ-LRKEE 415 +++G +TGIGL+H RDIV G I + +S+ G +F++ +PT+Q LRKEE Sbjct: 370 DQQGNPSTGIGLTHARDIVQSLHGHIQL-VSSNPGQTIFRLTIPTSQLLRKEE 421 >C2VA57.1 C2VA57_BACCE Sensor histidine kinase Length = 432 Score = 216 bits (550), Expect = 4e-54, Method: Compositional matrix adjust. Identities = 123/410 (30%), Positives = 218/410 (53%), Gaps = 8/410 (1%) Query: 8 VAGEFKINPFDGDIFRIXXXXXXXXXXXXXXXXXPYVITGVATGIVVLLFRTAMDAVWXX 67 +AGE +PF+ D+FR+ P GV GI V+LFR ++D V Sbjct: 26 IAGELNFHPFN-DMFRVSFGTPLFFFLLLFLRRIPAAAAGVLVGISVVLFRVSLDWVLQG 84 Query: 68 XXXXXXXXXXHFSAMVYYIVFALLMNLIKSRIDSFH--PLVLGGVAALIDLLSNEMELLT 125 + YY ++ L + R++ FH P+V+G + I+++++ EL Sbjct: 85 SFHMTESFYLRYPVFFYYFIYGSLFSF--CRVNKFHQKPIVIGCLGISIEIIASMSELAF 142 Query: 126 RLIVLDSATFRLNEWTYLMAIAVLRTYFTTGVYSSISVSQLRITQREQTRRIEQMLGFGS 185 +++ T ++E L+ IA+ R++F G + +++ + ++ + + + E ML + S Sbjct: 143 YHMLVLGTTITVSEVNKLIIIAIFRSFFALGFLNMMNLYETKLKESQVRKENENMLMYLS 202 Query: 186 GLYGEVFYLEKSIGTLENVTLSSYELYRNLKAEEEMPRYSRQVLEITQQIHEVKKDSQRI 245 LY E +L+K++ E +T +Y+LYRNL+A +++ S+ L+I ++HE+KKD+QRI Sbjct: 203 NLYVESVHLKKTLQNAELITQEAYQLYRNLQANDDLN--SKTALKIAGEVHEIKKDNQRI 260 Query: 246 LAGLVKLA-EREVTGDMPISDILRFTVKSNANYAEMLGKQISFSIQHTSDYTTDSYIPXX 304 AGL KL ++ V + ++ V+ N YAEMLGK I FS ++ Sbjct: 261 FAGLSKLLLDKNVAEYVEGHELAEIIVRINEKYAEMLGKDIRFSKHIEGEHAAYHVYTVL 320 Query: 305 XXXXXXXXXXVEVIQGKGSISLDMYEKEDNTLFTVTDSGSGIPERDLELVFEPGFTTKFN 364 VE I+ +G IS+ +Y+++ + F V D G GI ++ +LVF+PGFT+K++ Sbjct: 321 SIFNNLVANAVEAIEDRGLISIKLYKRDKHIFFEVIDDGPGIAQKYKKLVFKPGFTSKYD 380 Query: 365 EEGVAATGIGLSHVRDIVHLFAGEITVQPASHSGGAMFQIIVPTAQLRKE 414 + G +TGIGLS++ ++V GE+ ++ GG F + +P L++E Sbjct: 381 QTGTPSTGIGLSYINEMVTELGGEVRLEERETGGGCKFIVCLPECSLKRE 430 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.321 0.136 0.381 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,002,035,913 Number of extensions: 74713184 Number of successful extensions: 273804 Number of sequences better than 1.0e-05: 326 Number of HSP's gapped: 279903 Number of HSP's successfully gapped: 326 Length of query: 415 Length of database: 3,060,696,203 Length adjustment: 138 Effective length of query: 277 Effective length of database: 1,760,596,133 Effective search space: 487685128841 Effective search space used: 487685128841 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)