BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0315.0003.p (256 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KAC9.1 B3KAC9_9BACI Cobalamin (Vitamin B12) biosynthesis CbiX ... 336 1e-90 B1D900.1 B1D900_9BACL Cobalamin (Vitamin B12) biosynthesis CbiX ... 260 8e-68 C0ZHX2.1 C0ZHX2_BREBN Putative uncharacterized protein 187 7e-46 Q8TKD6.1 Q8TKD6_METAC Putative uncharacterized protein 139 2e-31 C1T880.1 C1T880_9FIRM Uncharacterized conserved protein 138 5e-31 >B3KAC9.1 B3KAC9_9BACI Cobalamin (Vitamin B12) biosynthesis CbiX protein Length = 254 Score = 336 bits (862), Expect = 1e-90, Method: Compositional matrix adjust. Identities = 162/251 (64%), Positives = 203/251 (80%), Gaps = 1/251 (0%) Query: 2 KPGILIISHGSRDKTWVSMVDEAVSHLTLGEELNVEASFLELVEGRLIQDGIDRLEDAGV 61 KPG+L+ISHGSR++ WVS+VDEAV L ++ V +S+LELVEGRLIQDGI LED GV Sbjct: 3 KPGVLVISHGSRERKWVSLVDEAVHRLASRMDIPVVSSYLELVEGRLIQDGITALEDQGV 62 Query: 62 TDIIVIPLFVSSGSTHVDEIEYALGAKAAPEKETELERFAVKAKVHYGYPVDDDPDIAVM 121 TDI+VIPLFVSSGSTHVDEI YA+GAKA P+ ET+L F V+++VH+G P+DDDP+IA M Sbjct: 63 TDIVVIPLFVSSGSTHVDEIAYAIGAKAVPDMETDLAPFRVRSRVHFGTPMDDDPEIAAM 122 Query: 122 IWDKLRGLSQHPERETILLVGHGSRHEGFRQRWQQGISSLAERVRRISGAAAADYGLLNP 181 +WDK++ LS +P +E ILLVGHGSRH+ FR RW++GI+SLA+RV +SG ADY LL+P Sbjct: 123 VWDKVKELSANPAKEVILLVGHGSRHDLFRSRWEKGINSLAQRVSEVSG-TGADYALLSP 181 Query: 182 DSVRGKVEYWREQGHEVLVAPLFLSEGYFTKVVIPERLRDLEYAYSGQTLLPHPLLPHWI 241 VR KV W E+G +V+VAPLFLSEGYFT+ VIP+RL+ LE YSG+TLLPHPLLP W+ Sbjct: 182 GDVRDKVTAWMERGCDVIVAPLFLSEGYFTEKVIPDRLQGLECRYSGRTLLPHPLLPQWM 241 Query: 242 ERQVKLMLEQL 252 E Q K +L+ L Sbjct: 242 ESQAKRLLQSL 252 >B1D900.1 B1D900_9BACL Cobalamin (Vitamin B12) biosynthesis CbiX protein Length = 257 Score = 260 bits (665), Expect = 8e-68, Method: Compositional matrix adjust. Identities = 133/256 (51%), Positives = 176/256 (68%), Gaps = 5/256 (1%) Query: 1 MKPGILIISHGSRDKTWVSMVDEAVSHLTLGEELN---VEASFLELVEGRLIQDGIDRLE 57 MKPGIL+ISHGSR+ WV +VDEAV+ L V +SFLE+VEGRLIQDGID LE Sbjct: 1 MKPGILVISHGSREAEWVRLVDEAVAAAAASPHLQGVPVYSSFLEIVEGRLIQDGIDALE 60 Query: 58 DAGVTDIIVIPLFVSSGSTHVDEIEYALGAKAAPEKETELERFAVKAKVHYGYPVDDDPD 117 + GVTD+ V+PLF+SSGSTHVDEI A G E E +L F V AKVHYG P+DDDPD Sbjct: 61 EQGVTDLFVLPLFLSSGSTHVDEIGQAFGLPPVAELEGDLGIFRVNAKVHYGMPIDDDPD 120 Query: 118 IAVMIWDKLRGLSQHPERETILLVGHGSRHEGFRQRWQQGISSLAERVRRISGAAAADYG 177 IA ++ ++ LS PE ET++L+ HGSR + F +RWQ G++ LAER+R++ G A + Sbjct: 121 IAELLLSNIKQLSIQPEAETVMLIAHGSREKVFHERWQHGLNQLAERLRKLGGFAHSATA 180 Query: 178 LLNPDSVRGKVEYWREQ--GHEVLVAPLFLSEGYFTKVVIPERLRDLEYAYSGQTLLPHP 235 +L PD + +E+ V+V PLFLS+GYFT VIP RL+ L+Y Y+GQ +LPHP Sbjct: 181 MLLPDQAAAHLRALQEERPSEAVIVVPLFLSKGYFTNTVIPSRLQGLDYRYNGQAMLPHP 240 Query: 236 LLPHWIERQVKLMLEQ 251 + W+ER++ L++ Sbjct: 241 AIERWLERRMTAWLQE 256 >C0ZHX2.1 C0ZHX2_BREBN Putative uncharacterized protein Length = 270 Score = 187 bits (476), Expect = 7e-46, Method: Compositional matrix adjust. Identities = 97/248 (39%), Positives = 153/248 (61%), Gaps = 3/248 (1%) Query: 2 KPGILIISHGSRDKTWVSMVDEAVSHLTLGEELNVEASFLELVEGRLIQDGIDRLEDAGV 61 K +L+I+HGS D W+++V+ AV +L + ++L VEGR I D ++RLE +G Sbjct: 3 KIAVLVIAHGSPDPDWLALVESAVWQCR--TDLPIRVAYLGGVEGRSISDEVNRLEASGA 60 Query: 62 TDIIVIPLFVSSGSTHVDEIEYALGAKAAPEKETELERFAVKAKVHYGYPVDDDPDIAVM 121 T I+V+PLF ++GS HV EI LG P ET+L R V+A++ + P++D ++ + Sbjct: 61 TTIVVVPLFATAGSNHVGEIRAMLGLDPWPTGETDLIRVPVQARILWCPPLEDHGNVEQI 120 Query: 122 IWDKLRGLSQHPERETILLVGHGSRHEGFRQRWQQGISSLAERVRRISGAAAADYGLLNP 181 + +++ LS+ P RE +LLVGHGS G +RW++ + L R++ AAA Y L P Sbjct: 121 VAERIASLSRVPSREALLLVGHGSDLPGCHERWEKLLLRLTFRLQNRYAFAAAGYATLRP 180 Query: 182 DSVRGKVEYWREQGHEVLVAPLFLSEGYFTKVVIPERLRDLEYAYSGQTLLPHPLLPHWI 241 D++ + ++G +VLV PLF+S+GYFT+ IP+RL L Y Y G LPHPL+ W+ Sbjct: 181 DTLYEQACSLAQKG-DVLVLPLFVSQGYFTRQAIPKRLAGLAYRYEGSAYLPHPLIADWM 239 Query: 242 ERQVKLML 249 + +++ L Sbjct: 240 SQSIRMAL 247 >Q8TKD6.1 Q8TKD6_METAC Putative uncharacterized protein Length = 4226 Score = 139 bits (351), Expect = 2e-31, Method: Composition-based stats. Identities = 83/242 (34%), Positives = 132/242 (54%), Gaps = 13/242 (5%) Query: 4 GILIISHGSRDKTWVSMVDEAVSHLTLGEELNVEASFLELVEGRLIQDGIDRLEDAGVTD 63 GIL+++HGS + W V +AV + VE FLE VEG I + LE+ GV Sbjct: 417 GILVVAHGSPEDEWNQPVRDAVEGIDC--PYPVELGFLEFVEGEDIGTAVASLEEQGVEH 474 Query: 64 IIVIPLFVSSGSTHVDEIEYALGAKAAPEKETELER----FAVKAKVHYGYPVDDDPDIA 119 II +P+FV+S S H++EI+Y LG ++ +E +E + A++ +DD P +A Sbjct: 475 IITVPIFVASASGHIEEIKYILGISSSITEEDAIEEGLEVVSHNAEIEMTSALDDHPLVA 534 Query: 120 VMIWDKLRGLSQHPERETILLVGHGSRHEGFRQRWQQGISSLAERVRRISGAAAADYGL- 178 ++ D++ +SQ ++E ++LV HG+ E + W+ ++SL ++++ DYG Sbjct: 535 EILDDRIATVSQQADQEIVVLVAHGTSEEEDLKVWKNNLASLGQQLKENYQFLDVDYGFV 594 Query: 179 -LNPDSVRGKVEYWREQ--GHEVLVAPLFLSEGYFTKVVIPERLRDLEYAYSGQ---TLL 232 + +VR VE + + G V+V P+ LSEG FT IPE L L Y Y + +LL Sbjct: 595 AMGEPNVRAVVEAQQAENPGASVIVMPVMLSEGTFTSTTIPEVLSGLTYLYPAEGQRSLL 654 Query: 233 PH 234 PH Sbjct: 655 PH 656 Score = 136 bits (343), Expect = 2e-30, Method: Composition-based stats. Identities = 85/241 (35%), Positives = 130/241 (53%), Gaps = 12/241 (4%) Query: 4 GILIISHGSRDKTWVSMVDEAVSHLTLGEELNVEASFLELVEGRLIQDGIDRLEDAGVTD 63 GIL+++HGS + W V +AV + VE FLE VEG I + LE+ GV Sbjct: 1988 GILVVAHGSPEDEWNQPVRDAVEGIDC--PYPVELGFLEFVEGEDIGTAVASLEEQGVER 2045 Query: 64 IIVIPLFVSSGSTHVDEIEYALGAKAAPEKETE---LERFAVKAKVHYGYPVDDDPDIAV 120 II +P+FV+S S H++EI+Y LG +A +E LE + A++ +DD P +A Sbjct: 2046 IITVPIFVASASDHIEEIKYILGISSAITEEASEEGLEVVSHSAEIEMTSALDDHPLVAE 2105 Query: 121 MIWDKLRGLSQHPERETILLVGHGSRHEGFRQRWQQGISSLAERVRRISGAAAADYGL-- 178 ++ D++ +SQ +E ++L+ HG+ E W+ ++SL ++++ DYG Sbjct: 2106 ILGDRIATVSQQAGQEIVVLIAHGTSAEADLAVWKNNLASLGQQLKENYQFLDVDYGFVA 2165 Query: 179 LNPDSVRGKVEYWREQ--GHEVLVAPLFLSEGYFTKVVIPERLRDLEYAY--SGQ-TLLP 233 L + R VE + + G ++V P+ LSEG FT IPE L L Y Y GQ +LLP Sbjct: 2166 LGEPNARAVVEAKQAENPGASIIVMPVMLSEGTFTGTKIPEVLAGLTYVYPEEGQRSLLP 2225 Query: 234 H 234 H Sbjct: 2226 H 2226 Score = 128 bits (321), Expect = 6e-28, Method: Composition-based stats. Identities = 85/249 (34%), Positives = 136/249 (54%), Gaps = 15/249 (6%) Query: 4 GILIISHGSRDKTWVSMVDEAVSHLTLGEELNVEASFLELVEGRLIQDGIDRLEDAGVTD 63 GIL+I+HGS D+ W V E+V+ + + VE FL+ E I + LE+ GV Sbjct: 3349 GILVIAHGS-DENWNQRVRESVAEVEV--PYPVELGFLD-AETENIAMAVQALEEKGVKR 3404 Query: 64 IIVIPLFVSSGSTHVDEIEYALGAKA----APEKETELERFAVKAKVHYGYPVDDDPDIA 119 I+ +P+FV S S+H++EI+Y LG + A +E LER V +DD IA Sbjct: 3405 IVAVPIFVCSASSHMEEIKYMLGLPSTLEDAAAQEEGLERIEKTTTVELTSALDDHLLIA 3464 Query: 120 VMIWDKLRGLSQHPERETILLVGHGSRHEGFRQRWQQGISSLAERVRRISGAAAADYGLL 179 ++ ++L+ LSQ E+E ++LV HG+ + WQ+ ++SL +++++I G YG + Sbjct: 3465 EILDERLKNLSQDAEKEIVVLVAHGTSNPENLAVWQEKMASLGDKLQQIQGFKQVYYGFV 3524 Query: 180 NPDS--VRGKVEYWREQGHE--VLVAPLFLSEGYFTKVVIPERLRDLEYAY--SGQ-TLL 232 + +R VE ++ + V+V P+ LSEG +T IP L + Y Y +GQ LL Sbjct: 3525 GAGTPNIRTVVEEVKKNNPDCTVIVMPVMLSEGVYTDSKIPTSLEGITYLYPDAGQRALL 3584 Query: 233 PHPLLPHWI 241 PH + +I Sbjct: 3585 PHANMAQYI 3593 Score = 58.2 bits (139), Expect = 6e-07, Method: Composition-based stats. Identities = 32/79 (40%), Positives = 45/79 (56%), Gaps = 2/79 (2%) Query: 4 GILIISHGSRDKTWVSMVDEAVSHLTLGEELNVEASFLELVEGRLIQDGIDRLEDAGVTD 63 GILI++ GS + W + +AV + VE FLE VEG I I LE+ GV Sbjct: 4150 GILIVAPGSPEDEWNQPIRDAVEAIDC--PYPVELGFLEFVEGENIWTAIASLEEQGVEH 4207 Query: 64 IIVIPLFVSSGSTHVDEIE 82 II +P+ V+S S H++EI+ Sbjct: 4208 IITVPILVTSASGHIEEIK 4226 >C1T880.1 C1T880_9FIRM Uncharacterized conserved protein Length = 1208 Score = 138 bits (347), Expect = 5e-31, Method: Compositional matrix adjust. Identities = 97/266 (36%), Positives = 141/266 (53%), Gaps = 27/266 (10%) Query: 4 GILIISHGSRDKTWVSMVDEAVSHLTLGEELNVEASFLELVEGRLIQDGIDRLEDAGVTD 63 G+L+++HGS + +W + V +AV+ + L VE FLE E I ++ LE GV Sbjct: 34 GVLVVAHGSDEASWNATVQQAVAEIAL--PYPVELGFLEFTEPD-IHAAVEALEARGVDK 90 Query: 64 IIVIPLFVSSGSTHVDEIEYALGAKA-APEKE----------------TELERFAVKAKV 106 II +PLF+SS S H++EI+Y LG + PE E +L A++ Sbjct: 91 IIAVPLFISSYSNHIEEIKYVLGLRPDLPEAEEPAGGHPGGGGDGIEEEDLTPVDTGAEI 150 Query: 107 HYGYPVDDDPDIAVMIWDKLRGLSQHPERETILLVGHGSRHEGFRQRWQQGISSLAERVR 166 +DD +A ++ D+L+ +SQ+P E +LVGHG RQ+WQ+ SLA R++ Sbjct: 151 VLTPALDDHVMVAGILADRLKTISQNPSEEIAVLVGHGPDTGEGRQKWQETFESLAGRLK 210 Query: 167 RISGAAAADYG--LLNPDSVRGKVEYWREQGHEVLVAPLFLSEGYFTKVVIPER-LRDLE 223 + A YG L+ SVR V QG +VLV P+ LSEGYFT VIP L DL+ Sbjct: 211 ALLNLKDAGYGFALMGEPSVRDSVYKAVYQG-DVLVVPVMLSEGYFTDTVIPHTLLTDLD 269 Query: 224 YAY---SGQTLLPHPLLPHWIERQVK 246 Y Y + L+PH + +IE +V Sbjct: 270 YRYPDAGNRALMPHSNIARFIELRVN 295 Score = 120 bits (300), Expect = 2e-25, Method: Compositional matrix adjust. Identities = 85/254 (33%), Positives = 131/254 (51%), Gaps = 13/254 (5%) Query: 4 GILIISHGSRDKTWVSMVDEAVSHLTLGEELNVEASFLELVEGRLIQDGIDRLEDAGVTD 63 GIL+++HGS +W V +AV ++ VEA FLE V G+ I I +LE GV Sbjct: 467 GILVLAHGSGQDSWNQPVIDAVQNVK--SPFPVEAGFLESVPGKDIPTAIRKLEAQGVER 524 Query: 64 IIVIPLFVSSGSTHVDEIEYALG--AKAAPEKETE--LERFAVKAKVHYGYPVDDDPDIA 119 II +P+FV+S S H++EI+Y LG + PE+ LE A + +DD +A Sbjct: 525 IIAVPIFVASASGHIEEIKYMLGLPSSITPEEAVAEGLEPIQHSAVIELTPALDDHILVA 584 Query: 120 VMIWDKLRGLSQHPERETILLVGHGSRHEGFRQRWQQGISSLAERVRRISGAAAADYGL- 178 ++ D++ +SQ +E ++L HG+ W+ ++SL ++++ G DYG Sbjct: 585 GILNDRIASVSQQAAQEVVVLAAHGTSSTEELAVWKHNLTSLGQKLKEKHGFLDVDYGFA 644 Query: 179 -LNPDSVRGKVEYWREQ--GHEVLVAPLFLSEGYFTKVVIPERLRDLEYAY--SGQ-TLL 232 + +R VE + G ++V P+ LSEG T IP L LEY Y +GQ +LL Sbjct: 645 AVGEPGLRAVVEDRQTAHPGASLIVMPVMLSEGTLTSNKIPSVLAGLEYIYPTAGQRSLL 704 Query: 233 PHPLLPHWIERQVK 246 PH + H I + Sbjct: 705 PHNNISHLISARAN 718 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.138 0.412 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,636,367,931 Number of extensions: 70637463 Number of successful extensions: 168928 Number of sequences better than 1.0e-05: 19 Number of HSP's gapped: 168984 Number of HSP's successfully gapped: 24 Length of query: 256 Length of database: 3,060,696,203 Length adjustment: 133 Effective length of query: 123 Effective length of database: 1,807,701,208 Effective search space: 222347248584 Effective search space used: 222347248584 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 130 (54.7 bits)