BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0315.0002.p (281 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1D901.1 B1D901_9BACL Diacylglycerol kinase catalytic region 355 2e-96 C5D535.1 C5D535_9BACI Diacylglycerol kinase catalytic region 283 2e-74 Q2BBZ5.1 Q2BBZ5_9BACI Putative uncharacterized protein 282 3e-74 C0Z4F8.1 C0Z4F8_BREBN Diacylglycerol kinase EC=2.7.1.107 276 2e-72 A4IK27.1 A4IK27_GEOTN Putative uncharacterized protein 275 4e-72 >B1D901.1 B1D901_9BACL Diacylglycerol kinase catalytic region Length = 304 Score = 355 bits (912), Expect = 2e-96, Method: Compositional matrix adjust. Identities = 175/278 (62%), Positives = 207/278 (74%), Gaps = 1/278 (0%) Query: 1 MKKRLADILDRLDIGGIEATCHXXXXXXXXXXXXXXXXXXXCYDLIIAAGGDGTLNEVIN 60 MK+RL DIL +LD GIE +CH YDLIIAAGGDGT+ EVIN Sbjct: 17 MKRRLPDILQKLDQAGIETSCHATIGAGDATIAAADAAERD-YDLIIAAGGDGTIYEVIN 75 Query: 61 GMAEKXXXXXXXXXXXXXXXDFARAMGIPKNWEDSCDLILRQESRLIDLGKANDRYFINI 120 GMA K DFARAMGIPK+WE +CDLI++ +R ID+G+AN+RYFINI Sbjct: 76 GMANKPNRPPLGILPLGTTNDFARAMGIPKHWEYACDLIIQNYTRPIDVGQANERYFINI 135 Query: 121 AGGGRLTELTYEVPSRLKTMMGQLAYYLKGIEKMASLSPQELYIRANGQELIHDEFMLFL 180 AGGG LTELTY+VP +LKTM+GQLAYYLKG+EKM LSP EL I A G H+EFMLFL Sbjct: 136 AGGGSLTELTYDVPIKLKTMIGQLAYYLKGLEKMTRLSPTELVIEAEGVGKFHEEFMLFL 195 Query: 181 IANTNSVGGFEKLAPDARIDDGLFDVIGVKKCNLAEFIRLVSLAIRGEHLQDKKVVYFRT 240 I N+NSVGGFEKLAPDA+ DDGLFDV+ +++CNLAEFIR+ +LA+RGEHL D V++FRT Sbjct: 196 ICNSNSVGGFEKLAPDAKPDDGLFDVVLLRRCNLAEFIRIATLALRGEHLNDSHVIHFRT 255 Query: 241 DAMEVTSPGYVQLNLDGELGGTLPGQFSILPQHLQIFA 278 + M+VTSP YVQ+NLDGE GGTLP FS+LP HLQIFA Sbjct: 256 NKMKVTSPSYVQINLDGEYGGTLPFTFSVLPSHLQIFA 293 >C5D535.1 C5D535_9BACI Diacylglycerol kinase catalytic region Length = 308 Score = 283 bits (723), Expect = 2e-74, Method: Compositional matrix adjust. Identities = 143/283 (50%), Positives = 192/283 (67%), Gaps = 6/283 (2%) Query: 1 MKKRLADILDRLDIGGIEATCHXXXXXXXXXXXXXXXXXXXCYDLIIAAGGDGTLNEVIN 60 KK L D+L +L+ G E +CH +DL++AAGGDGT+NEV+N Sbjct: 17 FKKHLPDVLVKLEKAGYETSCHATEGAGDATEAARKAVERE-FDLVVAAGGDGTINEVVN 75 Query: 61 GMAEKXXXXXXXXXXXXXXXDFARAMGIPKNWEDSCDLILRQESRLIDLGKAND----RY 116 G+A++ DFARA+G+P++ E +CD+I E+ ID+G + RY Sbjct: 76 GIADQDYRPKLGIIPVGTTNDFARAIGVPRSIERACDIIANGEAVPIDIGSVTNEGKTRY 135 Query: 117 FINIAGGGRLTELTYEVPSRLKTMMGQLAYYLKGIEKMASLSPQELYIRANGQELIHDEF 176 FINIAGGGRLTELTYEVPS+LKTM+GQLAYYLKGIE + S+ E I +G+ + E Sbjct: 136 FINIAGGGRLTELTYEVPSKLKTMLGQLAYYLKGIEMLPSIKATEARIEYDGK-MFEGEI 194 Query: 177 MLFLIANTNSVGGFEKLAPDARIDDGLFDVIGVKKCNLAEFIRLVSLAIRGEHLQDKKVV 236 MLFL++ TNSVGGFEKLAPD+ ++DG+FD+I +KK NLAEFIRLV+LA RGEH+ D ++ Sbjct: 195 MLFLVSLTNSVGGFEKLAPDSSLNDGMFDLIILKKTNLAEFIRLVTLAARGEHINDPHLI 254 Query: 237 YFRTDAMEVTSPGYVQLNLDGELGGTLPGQFSILPQHLQIFAQ 279 Y + + ++VTSP +QLNLDGE GG LPG+F L +HL++F Sbjct: 255 YTKANRIKVTSPNNMQLNLDGEYGGMLPGEFVNLYRHLEVFVS 297 >Q2BBZ5.1 Q2BBZ5_9BACI Putative uncharacterized protein Length = 325 Score = 282 bits (721), Expect = 3e-74, Method: Compositional matrix adjust. Identities = 142/277 (51%), Positives = 192/277 (69%), Gaps = 2/277 (0%) Query: 1 MKKRLADILDRLDIGGIEATCHXXXXXXXXXXXXXXXXXXXCYDLIIAAGGDGTLNEVIN 60 KK LA++L +L+ G E +CH YDL+IAAGGDGT+NEV+N Sbjct: 33 FKKNLAEVLQKLEEAGYETSCHATTCEGDATKAARVAVERK-YDLVIAAGGDGTINEVVN 91 Query: 61 GMAEKXXXXXXXXXXXXXXXDFARAMGIPKNWEDSCDLILRQESRLIDLGKANDRYFINI 120 GMAE+ DFARA+ IP++ + + D+I++ ++ +D+G+ ND+YFINI Sbjct: 92 GMAEQEYRPKLGIIPCGTTNDFARAVHIPRDVQAAVDIIIQGDTIPVDIGRFNDKYFINI 151 Query: 121 AGGGRLTELTYEVPSRLKTMMGQLAYYLKGIEKMASLSPQELYIRANGQELIHDEFMLFL 180 AGGGRLTELTYEVPS+LKTM+GQLAYYLKGIE + S+ E+ I +G+ L E MLFL Sbjct: 152 AGGGRLTELTYEVPSKLKTMLGQLAYYLKGIEMLPSIKSTEVSIEYDGK-LYEGEAMLFL 210 Query: 181 IANTNSVGGFEKLAPDARIDDGLFDVIGVKKCNLAEFIRLVSLAIRGEHLQDKKVVYFRT 240 + TNSVGGFE+LAPDA I+DGLF ++ +KK NLA+FIR+ S+A+RGEH+ D V+Y + Sbjct: 211 VGLTNSVGGFERLAPDASINDGLFSLLILKKTNLADFIRIASMAVRGEHIHDPHVIYTQA 270 Query: 241 DAMEVTSPGYVQLNLDGELGGTLPGQFSILPQHLQIF 277 + ++VTS VQLNLDGE GG LP +F L +HL++F Sbjct: 271 NRIKVTSKEKVQLNLDGEFGGLLPAEFVNLYRHLEVF 307 >C0Z4F8.1 C0Z4F8_BREBN Diacylglycerol kinase EC=2.7.1.107 Length = 296 Score = 276 bits (705), Expect = 2e-72, Method: Compositional matrix adjust. Identities = 137/279 (49%), Positives = 190/279 (68%), Gaps = 3/279 (1%) Query: 1 MKKRLADILDRLDIGGIEATCHXXXXXXXXXXXXXXXXXXXCYDLIIAAGGDGTLNEVIN 60 +++RL +ILD ++ G E +CH +D+I+AAGGDGT+ EV+N Sbjct: 17 VRRRLPEILDLMESAGYETSCHATRGEDDATEEAARAVARG-FDVILAAGGDGTIYEVVN 75 Query: 61 GMAEKXXXXXXXXXXXXXXXDFARAMGIPKNWEDSCDLILRQESRLIDLGKANDRYFINI 120 GMAE DFARA+GIPK+ + ++I + + + IDLG+ N+RYF+NI Sbjct: 76 GMAEHKARPSLGIIPCGTSNDFARAVGIPKSITRATEIITKGKKKRIDLGRINNRYFMNI 135 Query: 121 AGGGRLTELTYEVPSRLKTMMGQLAYYLKGIEKMASLSPQELYIRANGQELIHDEFMLFL 180 AGGG LT LTYEVPS+LKT++GQ+AYY+KG+EK+ SL P + + + + L+ +E M+FL Sbjct: 136 AGGGSLTNLTYEVPSKLKTLIGQMAYYVKGLEKLPSLHPIRVRLESRREVLLDEEIMVFL 195 Query: 181 IANTNSVGGFEKLAPDARIDDGLFDVIGVKKCNLAEFIRLVSLAIRGEHLQDKKVVYFRT 240 IAN+ SVGGF++LAPDA + DG DVI VKK N+AEFIRL + A+RGEHL+D ++YF+ Sbjct: 196 IANSRSVGGFDRLAPDADLSDGKLDVIVVKKMNIAEFIRLATQAVRGEHLKDPNILYFQA 255 Query: 241 DAMEVTSPG--YVQLNLDGELGGTLPGQFSILPQHLQIF 277 D ++ TSPG VQLNLDGELGG LP LP H+++F Sbjct: 256 DYIKATSPGGEKVQLNLDGELGGQLPCVVEALPGHIELF 294 >A4IK27.1 A4IK27_GEOTN Putative uncharacterized protein Length = 306 Score = 275 bits (702), Expect = 4e-72, Method: Compositional matrix adjust. Identities = 143/281 (50%), Positives = 187/281 (66%), Gaps = 8/281 (2%) Query: 1 MKKRLADILDRLDIGGIEATCHXXXXXXXXXXXXXXXXXXXCYDLIIAAGGDGTLNEVIN 60 K+ L D+L RL+ G E +CH +DL++AAGGDGT+NEV+N Sbjct: 17 FKRHLPDVLVRLEKAGYETSCHATEGPGDATKAARQAAERE-FDLVVAAGGDGTINEVVN 75 Query: 61 GMAEKXXXXXXXXXXXXXXXDFARAMGIPKNWEDSCDLILRQESRLIDLGKAND----RY 116 G+A + DFARA+G+P++ E +C++I E ID+G + RY Sbjct: 76 GIANQPYRPKLGVIPVGTTNDFARAIGVPRSIEGACEVIATGEPVAIDIGSVTNEDQTRY 135 Query: 117 FINIAGGGRLTELTYEVPSRLKTMMGQLAYYLKGIEKMASLSPQELYIRANGQELIHDEF 176 FINIAGGGRLTELTYEVPS+LKTM+GQLAYYLKGIE + S+ E I +G+ L E Sbjct: 136 FINIAGGGRLTELTYEVPSKLKTMLGQLAYYLKGIEMLPSIKATEAQIEYDGK-LFEGEI 194 Query: 177 MLFLIANTNSVGGFEKLAPDARIDDGLFDVIGVKKCNLAEFIRLVSLAIRGEHLQDKKVV 236 M+FL++ TNSVGGFEKLAPD+ ++DGLFD I VKK NLAEFIRLV+LA RGEH+ D V+ Sbjct: 195 MMFLVSLTNSVGGFEKLAPDSSLNDGLFDFIIVKKTNLAEFIRLVTLAARGEHINDPHVI 254 Query: 237 YFRTDAMEVTSPGYVQLNLDGELGGTLPGQFSILPQHLQIF 277 Y + + ++V S +QLNLDGE GG LPG+F L +HL++F Sbjct: 255 YTKANRVKVRSS--MQLNLDGEFGGMLPGEFVNLYRHLEVF 293 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.322 0.141 0.412 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,449,847,333 Number of extensions: 53346353 Number of successful extensions: 101053 Number of sequences better than 1.0e-05: 1130 Number of HSP's gapped: 98777 Number of HSP's successfully gapped: 1130 Length of query: 281 Length of database: 3,060,696,203 Length adjustment: 134 Effective length of query: 147 Effective length of database: 1,798,280,193 Effective search space: 264347188371 Effective search space used: 264347188371 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 130 (54.7 bits)