BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0315.0001.p (525 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1D902.1 B1D902_9BACL RNA methyltransferase, TrmA family 659 0.0 B3KAD5.1 B3KAD5_9BACI RNA methyltransferase, TrmA family 597 e-169 Q9KF10.1 Y687_BACHD Uncharacterized RNA methyltransferase BH0687... 573 e-161 B7GFZ4.1 B7GFZ4_ANOFW Methyltransferase 562 e-158 A8FAS6.1 A8FAS6_BACP2 TrmA family RNA methyltransferase 558 e-157 >B1D902.1 B1D902_9BACL RNA methyltransferase, TrmA family Length = 499 Score = 659 bits (1700), Expect = 0.0, Method: Compositional matrix adjust. Identities = 319/503 (63%), Positives = 383/503 (76%), Gaps = 44/503 (8%) Query: 23 PVYKNDEVMLDIIGMTHEGEGVGRVEGFTLFVQGALPGEKVRAKVLKTKKQYGYAKLLEL 82 PV KNDEV++DIIG+TH+GEGVGR +G+TLF+QGALPGE+VRAKV+K KK YGYA+L EL Sbjct: 38 PVQKNDEVVVDIIGLTHDGEGVGRADGYTLFIQGALPGERVRAKVMKVKKAYGYARLQEL 97 Query: 83 VQASGDRIAPPCPIYDQCGGCQLQHMDYTAQLAWKRQLVVDNLQRIGKLQVAGAAGRGAD 142 + AS DR+ P C IYD+CGGCQLQHMDY AQL WKRQ VVD L+RIGKLQ+AG Sbjct: 98 LDASADRVVPVCGIYDKCGGCQLQHMDYAAQLNWKRQHVVDALERIGKLQIAG------- 150 Query: 143 KLEGALADTAGDSDSREDTGIKLAETXXXXXXXXXXXXXXXXIIVRPTLGMDEPWRYRNK 202 + E +GI V PT+GM++PWRYRNK Sbjct: 151 -------------EGEEASGI----------------------TVHPTVGMNDPWRYRNK 175 Query: 203 AQVPIGVTEGG--LVGGFYARGSHRIVDMETCLIQHEDNDAVVAAVKGIGRDLGITAYDE 260 AQVP+G + G L+ GFYA+GSHRI+D + C IQH+ ND VV VK IGR+LG++AYDE Sbjct: 176 AQVPVGASAGAGELIAGFYAKGSHRIIDTDQCHIQHDRNDEVVRIVKAIGRELGVSAYDE 235 Query: 261 ETGRGLLRHVVVKKAFRTGEMMLVLITNGRDIPHLDAWIGSIREALPQVESICQNVNTQR 320 E+G+GLLRHV+ + F +GE+M+VL+TNGR +P + W+ IR ALP+V+SI QNVNT+ Sbjct: 236 ESGKGLLRHVMTRTGFVSGEIMVVLVTNGRKLPQAELWVERIRAALPEVKSIVQNVNTRD 295 Query: 321 TNVIFGSETRVLWGRDVIYDYIGDVQFAISPRSFYQINPAQTEVLYGKTVEYAGLTGNET 380 TNVIFG ET+VLWG +VIYD + ++FAIS RSFYQ+NP+QT LY V+YA LTG E+ Sbjct: 296 TNVIFGDETKVLWGSEVIYDELDGIRFAISARSFYQVNPSQTIELYRTAVDYAQLTGKES 355 Query: 381 VIDAYCGIGTISLFLAQHAAKVYGVEIVPEAIEDARANAKLNGMNNVEFAVGASEDVIPT 440 VIDAYCGIGTISLFLA+ A +VYGVEIVPEAIEDA+ NA+LNG++N F G +E VIP Sbjct: 356 VIDAYCGIGTISLFLARKAGRVYGVEIVPEAIEDAKRNAELNGISNASFEAGPAEVVIPR 415 Query: 441 WREQGVTPDVIVVDPPRKGCDPRLLDTILAMKPERVVYVSCNPSTLARDLRVLEDGGYKT 500 WRE+G+ PDVIVVDPPRKGCDP LLDTIL M+PERVVYVSCNPSTLARDLR LEDGGYKT Sbjct: 416 WREEGIIPDVIVVDPPRKGCDPALLDTILKMQPERVVYVSCNPSTLARDLRTLEDGGYKT 475 Query: 501 VEVTPVDMFPHTVHVECCVSLER 523 VEVTPVDMFP T HVEC V + R Sbjct: 476 VEVTPVDMFPWTSHVECVVWMVR 498 >B3KAD5.1 B3KAD5_9BACI RNA methyltransferase, TrmA family Length = 400 Score = 597 bits (1539), Expect = e-169, Method: Compositional matrix adjust. Identities = 298/405 (73%), Positives = 329/405 (81%), Gaps = 13/405 (3%) Query: 121 VVDNLQRIGKLQVAGAAGRGADKLEGALADTAGDSDSREDTG----IKLAETXXXXXXXX 176 +VDNL+RIGKL+V E A+A D R T I+++ET Sbjct: 1 MVDNLERIGKLRVV---------RESAIAGETCVDDKRSGTDGGGDIEISETNGTNPAVS 51 Query: 177 XXXXXXXXIIVRPTLGMDEPWRYRNKAQVPIGVTEGGLVGGFYARGSHRIVDMETCLIQH 236 I+VRPTLGM EPWRYRNK+QVPIGVTEGGLVGGFYARGSHRIVDMETCLIQH Sbjct: 52 GVESDQEGILVRPTLGMSEPWRYRNKSQVPIGVTEGGLVGGFYARGSHRIVDMETCLIQH 111 Query: 237 EDNDAVVAAVKGIGRDLGITAYDEETGRGLLRHVVVKKAFRTGEMMLVLITNGRDIPHLD 296 E ND VV+ VK IGR LGITAY+EETG+GLLRHVVVK F T EMM+VL+TNG IP ++ Sbjct: 112 EQNDEVVSRVKAIGRKLGITAYNEETGQGLLRHVVVKIGFATREMMIVLVTNGERIPRVN 171 Query: 297 AWIGSIREALPQVESICQNVNTQRTNVIFGSETRVLWGRDVIYDYIGDVQFAISPRSFYQ 356 WI +IR+ LP V SICQNVNT++TNVIFG TRVLWGR+VI+DYIGDV+FAIS RSFYQ Sbjct: 172 EWIAAIRKELPAVVSICQNVNTRKTNVIFGDVTRVLWGREVIHDYIGDVKFAISARSFYQ 231 Query: 357 INPAQTEVLYGKTVEYAGLTGNETVIDAYCGIGTISLFLAQHAAKVYGVEIVPEAIEDAR 416 +NPAQTEVLYGKTVEYAGLTG ETVIDAYCGIGTISLFLAQHA KVYGVEIV EAIEDAR Sbjct: 232 VNPAQTEVLYGKTVEYAGLTGEETVIDAYCGIGTISLFLAQHAKKVYGVEIVKEAIEDAR 291 Query: 417 ANAKLNGMNNVEFAVGASEDVIPTWREQGVTPDVIVVDPPRKGCDPRLLDTILAMKPERV 476 ANA+LNGM +VEF VGASEDVIP W+EQG+ DVIVVDPPRKGCDPRLL+TIL MKPERV Sbjct: 292 ANAELNGMKHVEFEVGASEDVIPRWKEQGIEADVIVVDPPRKGCDPRLLETILEMKPERV 351 Query: 477 VYVSCNPSTLARDLRVLEDGGYKTVEVTPVDMFPHTVHVECCVSL 521 VYVSCNPSTLARDLR+LEDGGY+TVEVTPVDMFPHTVHVE L Sbjct: 352 VYVSCNPSTLARDLRILEDGGYRTVEVTPVDMFPHTVHVESVAVL 396 >Q9KF10.1 Y687_BACHD Uncharacterized RNA methyltransferase BH0687 EC=2.1.1.- Length = 458 Score = 573 bits (1478), Expect = e-161, Method: Compositional matrix adjust. Identities = 285/500 (57%), Positives = 353/500 (70%), Gaps = 51/500 (10%) Query: 23 PVYKNDEVMLDIIGMTHEGEGVGRVEGFTLFVQGALPGEKVRAKVLKTKKQYGYAKLLEL 82 PV KND V + I +TH+G GV +V+G+ LF+ ALPGE+++AKV+K KK YG+ ++L + Sbjct: 7 PVNKNDVVEVTIEDLTHDGAGVAKVDGYALFIPKALPGERLKAKVVKVKKGYGFGRVLNM 66 Query: 83 VQASGDRIAPPCPIYDQCGGCQLQHMDYTAQLAWKRQLVVDNLQRIGKLQVAGAAGRGAD 142 ++AS DR+ PCP+++QCGGCQLQHM Y AQL +K++ V D L+RIGK+ Sbjct: 67 IEASPDRVEAPCPVFNQCGGCQLQHMSYDAQLRYKQKQVQDVLERIGKI----------- 115 Query: 143 KLEGALADTAGDSDSREDTGIKLAETXXXXXXXXXXXXXXXXIIVRPTLGMDEPWRYRNK 202 + VRPT+GM+EPWRYRNK Sbjct: 116 ----------------------------------------TAVTVRPTIGMNEPWRYRNK 135 Query: 203 AQVPIGVTEGGLVGGFYARGSHRIVDMETCLIQHEDNDAVVAAVKGIGRDLGITAYDEET 262 AQVP+G EGGL+ GFY SHRI+DM+ C+IQHE+ND V+ VK + R+LGI YDEE Sbjct: 136 AQVPVGEREGGLIAGFYQERSHRIIDMDECMIQHEENDKVIRQVKELARELGIRGYDEEK 195 Query: 263 GRGLLRHVVVKKAFRTGEMMLVLITNGRDIPHLDAWIGSIREALPQVESICQNVNTQRTN 322 RG LRHVV + TGE+M+VLIT G ++PH I I +A+P V+SI QNVN +RTN Sbjct: 196 HRGTLRHVVARYGKNTGEIMVVLITRGEELPHKKTLIERIHKAIPHVKSIVQNVNPKRTN 255 Query: 323 VIFGSETRVLWGRDVIYDYIGDVQFAISPRSFYQINPAQTEVLYGKTVEYAGLTGNETVI 382 VIFG +T+VLWG + IYD IGD++FAIS RSFYQ+NP QT+VLY + +E+A LTG+ETVI Sbjct: 256 VIFGDKTKVLWGEEYIYDTIGDIKFAISARSFYQVNPEQTKVLYDQALEFANLTGSETVI 315 Query: 383 DAYCGIGTISLFLAQHAAKVYGVEIVPEAIEDARANAKLNGMNNVEFAVGASEDVIPTWR 442 DAYCGIGTISLFLAQ A VYGVEIVPEAI DA+ NA+LNG NV+FAVG +E V+P W Sbjct: 316 DAYCGIGTISLFLAQQAKHVYGVEIVPEAISDAKRNARLNGFANVQFAVGDAEKVMPWWY 375 Query: 443 EQGVTPDVIVVDPPRKGCDPRLLDTILAMKPERVVYVSCNPSTLARDLRVLEDGGYKTVE 502 QGV DVIVVDPPRKGCD LL TIL MKP+RVVYVSCNP+TLARDLRVLEDGGY+T + Sbjct: 376 AQGVRADVIVVDPPRKGCDEALLKTILNMKPDRVVYVSCNPATLARDLRVLEDGGYETKD 435 Query: 503 VTPVDMFPHTVHVECCVSLE 522 V PVDMFP T H+E LE Sbjct: 436 VQPVDMFPWTTHIESVAVLE 455 >B7GFZ4.1 B7GFZ4_ANOFW Methyltransferase Length = 456 Score = 562 bits (1448), Expect = e-158, Method: Compositional matrix adjust. Identities = 284/503 (56%), Positives = 347/503 (68%), Gaps = 51/503 (10%) Query: 23 PVYKNDEVMLDIIGMTHEGEGVGRVEGFTLFVQGALPGEKVRAKVLKTKKQYGYAKLLEL 82 PV KN+ + +TH+G GV +++GF +FV LPGEK + KV+K KK YGY +L+EL Sbjct: 4 PVAKNEYYDVIFEDLTHDGAGVAKIDGFPIFVPNGLPGEKAKVKVIKVKKGYGYGRLIEL 63 Query: 83 VQASGDRIAPPCPIYDQCGGCQLQHMDYTAQLAWKRQLVVDNLQRIGKLQVAGAAGRGAD 142 + S DR+ PC +Y QCGGCQLQH+ Y QL K + V + L RIGK++ Sbjct: 64 YEPSADRVEAPCTVYKQCGGCQLQHLSYEGQLKAKHKHVKEVLARIGKIE---------- 113 Query: 143 KLEGALADTAGDSDSREDTGIKLAETXXXXXXXXXXXXXXXXIIVRPTLGMDEPWRYRNK 202 + V P LGM++PWRYRNK Sbjct: 114 -----------------------------------------DVTVHPVLGMNDPWRYRNK 132 Query: 203 AQVPIGVTEGGLVGGFYARGSHRIVDMETCLIQHEDNDAVVAAVKGIGRDLGITAYDEET 262 AQVP+G EGGLV GFY SH I+DM+TCLIQ E ND VV VK I I AY+E+T Sbjct: 133 AQVPVGEREGGLVAGFYKERSHDIIDMDTCLIQQEMNDIVVQTVKQICEQYNIPAYNEQT 192 Query: 263 GRGLLRHVVVKKAFRTGEMMLVLITNGRDIPHLDAWIGSIREALPQVESICQNVNTQRTN 322 +GLLRH++ + T E+M+VLIT ++PH + + +I + +P V+SI QN+N +RTN Sbjct: 193 HKGLLRHIMARYGATTKEVMVVLITRTEELPHKNKIVEAIVKRVPNVKSIVQNINPKRTN 252 Query: 323 VIFGSETRVLWGRDVIYDYIGDVQFAISPRSFYQINPAQTEVLYGKTVEYAGLTGNETVI 382 VI G +TRVLWG + IYDYIGD+QFAIS RSFYQ+NP QT+VLY K +EYA LTG ETVI Sbjct: 253 VIMGEQTRVLWGSEYIYDYIGDIQFAISARSFYQVNPEQTKVLYEKALEYAELTGEETVI 312 Query: 383 DAYCGIGTISLFLAQHAAKVYGVEIVPEAIEDARANAKLNGMNNVEFAVGASEDVIPTWR 442 DAYCGIGTISLFLA++A KVYGVE+VPEAIEDA+ NA+LNGM NVEFAVG +E VIP W Sbjct: 313 DAYCGIGTISLFLAKNAKKVYGVEVVPEAIEDAKRNAELNGMTNVEFAVGEAEVVIPKWY 372 Query: 443 EQGVTPDVIVVDPPRKGCDPRLLDTILAMKPERVVYVSCNPSTLARDLRVLEDGGYKTVE 502 EQGV D IVVDPPRKGCD LL TI+AMKP+RVVYVSCNP+TLARDLR+LEDGGYKT+E Sbjct: 373 EQGVKADCIVVDPPRKGCDESLLQTIIAMKPKRVVYVSCNPATLARDLRILEDGGYKTLE 432 Query: 503 VTPVDMFPHTVHVECCVSLERTQ 525 V PVDMFPHTVHVEC V LE ++ Sbjct: 433 VQPVDMFPHTVHVECVVKLEVSK 455 >A8FAS6.1 A8FAS6_BACP2 TrmA family RNA methyltransferase Length = 465 Score = 558 bits (1439), Expect = e-157, Method: Compositional matrix adjust. Identities = 278/505 (55%), Positives = 336/505 (66%), Gaps = 52/505 (10%) Query: 23 PVYKNDEVMLDIIGMTHEGEGVGRVEGFTLFVQGALPGEKVRAKVLKTKKQYGYAKLLEL 82 PV KN+ + +THEG GV ++EGF +FV ALP E+ + KV + KK + + +L+EL Sbjct: 7 PVEKNEYYNVTFEDLTHEGAGVAKIEGFPVFVPNALPDEQGKIKVTRVKKGFAFGRLMEL 66 Query: 83 VQASGDRIAPPCPIYDQCGGCQLQHMDYTAQLAWKRQLVVDNLQRIGKLQVAGAAGRGAD 142 + S R PCPIY QCGGCQ+QHM Y QL +K++ V D L+RIGKL ++ Sbjct: 67 TKESQHRQDAPCPIYKQCGGCQIQHMSYEGQLLFKQKQVKDVLERIGKLDMS-------- 118 Query: 143 KLEGALADTAGDSDSREDTGIKLAETXXXXXXXXXXXXXXXXIIVRPTLGMDEPWRYRNK 202 + V P LGM++PW YRNK Sbjct: 119 -----------------------------------------RVKVHPVLGMEDPWNYRNK 137 Query: 203 AQVPIGVTEGGLVGGFYARGSHRIVDMETCLIQHEDNDAVVAAVKGIGRDLGITAYDEET 262 AQVP+G EGGLV GFY + +H I+DME CLIQ +ND VV AVK I + GI AY+EE Sbjct: 138 AQVPVGEREGGLVAGFYQQRTHEIIDMEKCLIQQSENDEVVQAVKEICQAFGIKAYNEER 197 Query: 263 GRGLLRHVVVKKAFRTGEMMLVLITNGRDIPHLDAWIGSIREALPQVESICQNVNTQRTN 322 +G LRHV+V+ TGEMM+V +T + P I I P + SI QNVN+++TN Sbjct: 198 HKGWLRHVMVRYGIATGEMMVVFVTRTAEFPQKQQVIEQIIARFPHIRSIVQNVNSKKTN 257 Query: 323 VIFGSETRVLWGRDVIYDYIGDVQFAISPRSFYQINPAQTEVLYGKTVEYAGLTGNETVI 382 VIFG ET VLWG + IYD IGD++FAIS RSFYQ+NPAQT++LY K +EYA L G ETVI Sbjct: 258 VIFGDETTVLWGEEYIYDTIGDIKFAISARSFYQVNPAQTKMLYDKALEYAELQGEETVI 317 Query: 383 DAYCGIGTISLFLAQHAAKVYGVEIVPEAIEDARANAKLNGMNNVEFAVGASEDVIPTWR 442 DAYCGIGTISLFLAQ A +VYGVEIVPEAIEDA+ NA LNG+NNVEFAVG +E VIP W Sbjct: 318 DAYCGIGTISLFLAQKAGRVYGVEIVPEAIEDAKRNAALNGINNVEFAVGEAETVIPNWY 377 Query: 443 EQGVTPDVIVVDPPRKGCDPRLLDTILAMKPERVVYVSCNPSTLARDLRVLEDGGYKTVE 502 ++G+ D +VVDPPRKGCD LLDTIL MKP+RVVYVSCNP TLARDLR+LEDGGY T E Sbjct: 378 KEGIKADTLVVDPPRKGCDEALLDTILKMKPKRVVYVSCNPGTLARDLRILEDGGYVTEE 437 Query: 503 VTPVDMFPHTVHVECCVSL---ERT 524 V PVDMFPHT HVEC L ERT Sbjct: 438 VQPVDMFPHTSHVECVAQLILRERT 462 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.138 0.411 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 3,020,826,347 Number of extensions: 126886537 Number of successful extensions: 336735 Number of sequences better than 1.0e-05: 2473 Number of HSP's gapped: 332323 Number of HSP's successfully gapped: 3929 Length of query: 525 Length of database: 3,060,696,203 Length adjustment: 140 Effective length of query: 385 Effective length of database: 1,741,754,103 Effective search space: 670575329655 Effective search space used: 670575329655 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 134 (56.2 bits)