BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0312.0008.p (322 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Q65P62.1 Q65P62_BACLD YbaS 290 2e-76 C0ZEB9.1 C0ZEB9_BREBN Conserved hypothetical membrane protein 288 4e-76 P55190.3 YBAS_BACSU Uncharacterized protein ybaS 273 1e-71 A7Z0T6.1 A7Z0T6_BACA2 YbaS 272 4e-71 B4AMY1.1 B4AMY1_BACPU Sodium Bile acid symporter family 271 8e-71 >Q65P62.1 Q65P62_BACLD YbaS Length = 325 Score = 290 bits (741), Expect = 2e-76, Method: Compositional matrix adjust. Identities = 152/322 (47%), Positives = 211/322 (65%), Gaps = 2/322 (0%) Query: 1 MLLAVNRTLNRMMPLITPLSVLTGVLCGSALSSYTYLSPWLFAFMTFAGSISLGFKDFVN 60 ML +N+TL ++MPL+TPLSV++GVL L+ L PW+FAFMTFAGS+ F+ Sbjct: 1 MLQTINKTLGKIMPLLTPLSVVSGVLLADYLNGLASLVPWIFAFMTFAGSLGANFQSLKT 60 Query: 61 VLKKPLPLFACLFILHIVMPLVALGIGNVLYHADEFTITGLVLAAVIPTGVSSFIWVSIY 120 + PLP+F LF+LHI MP++A G++++ D TITGL L VIPTG++S IW ++Y Sbjct: 61 AVAHPLPMFLALFVLHIFMPVLAWSTGHLVFSGDSLTITGLTLGVVIPTGITSMIWAAMY 120 Query: 121 KGNXXXXXXXXXXXXXXAPFVVPGVLSLLIGTSVELDTGAMMSSLFWMIVVPSLLGMVLN 180 KGN +PF+VP LS+L G VE+D MM L MIV+PSLLGM++N Sbjct: 121 KGNIGLTLSIILVDTILSPFIVPFSLSILAGAHVEMDVIGMMKGLVGMIVIPSLLGMLVN 180 Query: 181 EWTKGAVAPVWSPRLNPFSKLFMAAVIMINGSVISPYLADISLHLVGLAFVIVLLASTGY 240 +++ S PFSK+ +AAV+ IN SV++PYL ++ L + +A + L+A TGY Sbjct: 181 QFSTPERTVRLSANCAPFSKVGLAAVVAINSSVVAPYLKNVDLKFLTIAVTVFLVAFTGY 240 Query: 241 ALCFLTARVLRWNEADEVALIFNGGMRNISAGAVLAVSYFPAPVAVPVVLGMVFQQLLAS 300 +L R+++ + + VALIF GGMRNISAGAVLA S+FP VAVPVV+GM+FQQ+LA+ Sbjct: 241 LTAWLIGRLMKRPQEEIVALIFTGGMRNISAGAVLATSFFPPQVAVPVVIGMLFQQILAA 300 Query: 301 LAGFLL--SHRAKVRHTAEQTT 320 L G LL S K H ++T+ Sbjct: 301 LFGHLLARSELKKAHHLDKKTS 322 >C0ZEB9.1 C0ZEB9_BREBN Conserved hypothetical membrane protein Length = 324 Score = 288 bits (738), Expect = 4e-76, Method: Compositional matrix adjust. Identities = 150/321 (46%), Positives = 208/321 (64%) Query: 2 LLAVNRTLNRMMPLITPLSVLTGVLCGSALSSYTYLSPWLFAFMTFAGSISLGFKDFVNV 61 L +NR L ++MP++TP SV GV+ G+ L + +LSPW+FAFMTF GS+ GFK+F V Sbjct: 4 LAKLNRFLEKIMPILTPSSVAIGVIAGTHLQPFAFLSPWVFAFMTFTGSLGSGFKEFAKV 63 Query: 62 LKKPLPLFACLFILHIVMPLVALGIGNVLYHADEFTITGLVLAAVIPTGVSSFIWVSIYK 121 L +PLPL L ILH +MPLVA + + Y D ITG +LAA+IPTG++SF+W SIY Sbjct: 64 LTRPLPLIVNLLILHALMPLVAWSMARLFYPDDIHVITGFLLAALIPTGITSFLWSSIYF 123 Query: 122 GNXXXXXXXXXXXXXXAPFVVPGVLSLLIGTSVELDTGAMMSSLFWMIVVPSLLGMVLNE 181 GN +P +VP +SL +G +VE+D +M LF+MIV+PSL+GM+LN Sbjct: 124 GNIALTLSIILLDTMLSPLIVPLGMSLFLGATVEMDLAELMKGLFYMIVLPSLVGMLLNH 183 Query: 182 WTKGAVAPVWSPRLNPFSKLFMAAVIMINGSVISPYLADISLHLVGLAFVIVLLASTGYA 241 +KG V +P+L PFSK+ M V+ IN S++S Y + LVG+A +++++A GY Sbjct: 184 LSKGNVKKTLAPKLAPFSKMGMGVVVAINSSMVSSYFHMMDAKLVGMAVLVLVVAILGYL 243 Query: 242 LCFLTARVLRWNEADEVALIFNGGMRNISAGAVLAVSYFPAPVAVPVVLGMVFQQLLASL 301 + + A++ W V L FN GMRNISAGAV+A++YFPAPVA+PVVLGM+FQQ+LAS Sbjct: 244 MGWGIAKLFGWERDVIVTLTFNSGMRNISAGAVMAITYFPAPVALPVVLGMLFQQILAST 303 Query: 302 AGFLLSHRAKVRHTAEQTTAA 322 G L K Q ++ Sbjct: 304 FGKFLELVEKRPQPQPQELSS 324 >P55190.3 YBAS_BACSU Uncharacterized protein ybaS Length = 306 Score = 273 bits (699), Expect = 1e-71, Method: Compositional matrix adjust. Identities = 142/296 (47%), Positives = 193/296 (65%) Query: 13 MPLITPLSVLTGVLCGSALSSYTYLSPWLFAFMTFAGSISLGFKDFVNVLKKPLPLFACL 72 MPL+TP SV+ GVL L+ Y + PW+FAF+TFAGS+S F+ + L PLP+ L Sbjct: 1 MPLLTPTSVVLGVLLSQFLNGYEWAVPWIFAFITFAGSLSANFQSLRHALSHPLPMILAL 60 Query: 73 FILHIVMPLVALGIGNVLYHADEFTITGLVLAAVIPTGVSSFIWVSIYKGNXXXXXXXXX 132 F+LHI MPL A G G++++ D TITGL LA VIPTG++S IW ++YKGN Sbjct: 61 FVLHIFMPLFAWGSGHLIFKGDPLTITGLTLAVVIPTGITSLIWAAMYKGNVGLTLSIIL 120 Query: 133 XXXXXAPFVVPGVLSLLIGTSVELDTGAMMSSLFWMIVVPSLLGMVLNEWTKGAVAPVWS 192 +P +VP LSLL G V +D MM L M+V+PS LGM+ N+ + S Sbjct: 121 VDTVLSPLIVPLSLSLLAGAQVHMDVWGMMKGLIVMVVIPSFLGMLFNQMSSPERTAFVS 180 Query: 193 PRLNPFSKLFMAAVIMINGSVISPYLADISLHLVGLAFVIVLLASTGYALCFLTARVLRW 252 L+PFSKL + AVI IN S I+PY I L G+A + +A TGYA +L ++++ Sbjct: 181 SALSPFSKLCLMAVIAINSSAIAPYFKSIDLRFAGIAVTVFFIALTGYAAAWLIGKMMKR 240 Query: 253 NEADEVALIFNGGMRNISAGAVLAVSYFPAPVAVPVVLGMVFQQLLASLAGFLLSH 308 + + V+LIF GGMRNISAGAVLAV++FP+ VAVPVV+GM+FQQ+LA+L G++L+ Sbjct: 241 RQEEIVSLIFTGGMRNISAGAVLAVTFFPSQVAVPVVIGMLFQQILAALFGYMLNR 296 >A7Z0T6.1 A7Z0T6_BACA2 YbaS Length = 327 Score = 272 bits (695), Expect = 4e-71, Method: Compositional matrix adjust. Identities = 137/307 (44%), Positives = 204/307 (66%) Query: 2 LLAVNRTLNRMMPLITPLSVLTGVLCGSALSSYTYLSPWLFAFMTFAGSISLGFKDFVNV 61 L +N+ L R+MPL+TP SV GVL +S PW+FAF+TF GS+S F+ Sbjct: 4 LQKLNQVLGRIMPLLTPSSVAAGVLLSQHISWMAGAVPWIFAFITFTGSLSANFQSLKRS 63 Query: 62 LKKPLPLFACLFILHIVMPLVALGIGNVLYHADEFTITGLVLAAVIPTGVSSFIWVSIYK 121 ++KPLP+ LF+LHI MP+ A G G++++ D T+TGL++ VIPTG++S IW ++YK Sbjct: 64 IEKPLPMIMALFVLHIFMPVFAWGSGHLIFSGDPLTVTGLIMGVVIPTGITSLIWAAMYK 123 Query: 122 GNXXXXXXXXXXXXXXAPFVVPGVLSLLIGTSVELDTGAMMSSLFWMIVVPSLLGMVLNE 181 GN +PF+VP LS+L G +V++D MM L M+V+PS+ GM++N+ Sbjct: 124 GNVGLTLSIILVDTVLSPFIVPLSLSVLAGANVQMDVWGMMKGLAEMVVIPSIAGMIVNQ 183 Query: 182 WTKGAVAPVWSPRLNPFSKLFMAAVIMINGSVISPYLADISLHLVGLAFVIVLLASTGYA 241 ++ S L+PFSK+ + VI IN S I+PY+ + + G+A ++ +A TGYA Sbjct: 184 FSPPVRTQAISRSLSPFSKICLMIVIAINSSEIAPYVTHVDIKFAGIAGMVFFIAMTGYA 243 Query: 242 LCFLTARVLRWNEADEVALIFNGGMRNISAGAVLAVSYFPAPVAVPVVLGMVFQQLLASL 301 + +L R+++ ++ + V+LI+ GGMRNISAGAVLAVS+FP+ VAVPVV+GM+FQQ+LA+L Sbjct: 244 VAWLIGRLMKRSQDEIVSLIYTGGMRNISAGAVLAVSFFPSQVAVPVVIGMLFQQILAAL 303 Query: 302 AGFLLSH 308 G+LL Sbjct: 304 FGYLLRR 310 >B4AMY1.1 B4AMY1_BACPU Sodium Bile acid symporter family Length = 321 Score = 271 bits (692), Expect = 8e-71, Method: Compositional matrix adjust. Identities = 146/308 (47%), Positives = 205/308 (66%) Query: 1 MLLAVNRTLNRMMPLITPLSVLTGVLCGSALSSYTYLSPWLFAFMTFAGSISLGFKDFVN 60 ML+ +N+ L RMMPLITP+SVL GVL + L +T++ PW+FA MTF+GS+S F + Sbjct: 1 MLVRLNQVLGRMMPLITPISVLLGVLLAAYLKDFTFIVPWVFAIMTFSGSLSSTFSALKH 60 Query: 61 VLKKPLPLFACLFILHIVMPLVALGIGNVLYHADEFTITGLVLAAVIPTGVSSFIWVSIY 120 + PLPL ILH++MPL A G++++ D TITGL LA VIPTGVSS IW ++Y Sbjct: 61 TVMHPLPLILSFMILHVLMPLYAWSAGHLIFAGDSMTITGLTLAMVIPTGVSSLIWAAMY 120 Query: 121 KGNXXXXXXXXXXXXXXAPFVVPGVLSLLIGTSVELDTGAMMSSLFWMIVVPSLLGMVLN 180 KGN +P +VP LSL +GT V +D A+M LF M+V+PS+LGM++ Sbjct: 121 KGNVGLTLSIILIDTLLSPLIVPVSLSLFVGTQVHMDMVAIMKGLFGMVVLPSILGMLVR 180 Query: 181 EWTKGAVAPVWSPRLNPFSKLFMAAVIMINGSVISPYLADISLHLVGLAFVIVLLASTGY 240 ++ K +V S L PFSKL + V+ IN SV++PYL ++ A + +A +GY Sbjct: 181 QYAKPSVTKTMSQTLAPFSKLGLFVVVSINSSVVAPYLRELDAAFFSKAATVFFIAMSGY 240 Query: 241 ALCFLTARVLRWNEADEVALIFNGGMRNISAGAVLAVSYFPAPVAVPVVLGMVFQQLLAS 300 A +L +V++ + + VA+++ GGMRNISAGAVLA+S+FP VAVPVV+GM+FQQ+LA+ Sbjct: 241 AFAWLIGKVMKCTQGEIVAIVYTGGMRNISAGAVLAISFFPPAVAVPVVIGMLFQQVLAA 300 Query: 301 LAGFLLSH 308 L GFLL+ Sbjct: 301 LFGFLLNR 308 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.328 0.140 0.424 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,733,060,565 Number of extensions: 68135583 Number of successful extensions: 336318 Number of sequences better than 1.0e-05: 642 Number of HSP's gapped: 335729 Number of HSP's successfully gapped: 642 Length of query: 322 Length of database: 3,060,696,203 Length adjustment: 136 Effective length of query: 186 Effective length of database: 1,779,438,163 Effective search space: 330975498318 Effective search space used: 330975498318 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 131 (55.1 bits)