BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0311.0020.p (254 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1DED3.1 B1DED3_9BACL Glycosyl transferase, WecB/TagA/CpsF famil... 199 2e-49 C0Z749.1 C0Z749_BREBN Probable teichoic acid biosynthesis protei... 188 4e-46 B3K7I1.1 B3K7I1_9BACI Glycosyl transferase, WecB/TagA/CpsF famil... 185 3e-45 A4J962.1 A4J962_DESRM N-acetylmannosaminyltransferase EC=2.4.1.187 179 2e-43 C3H960.1 C3H960_BACTU Glycosyl transferase, WecB/TagA/CpsF 178 5e-43 >B1DED3.1 B1DED3_9BACL Glycosyl transferase, WecB/TagA/CpsF family EC=2.4.1.187 Length = 253 Score = 199 bits (506), Expect = 2e-49, Method: Compositional matrix adjust. Identities = 103/243 (42%), Positives = 154/243 (63%), Gaps = 11/243 (4%) Query: 11 TVSILGVRFSKLTLEETVLHLAEQIENKRDKLFHLITANPEIXXXXXXL--LQRIIQSAD 68 TV I G+ FSK++++ETV +L E IE+KR H+ITANP + ++++ A+ Sbjct: 7 TVPIYGIPFSKMSMDETVKYLTEAIEHKRQT--HVITANPIMVMSAVESQDYYKMMRKAE 64 Query: 69 MVVPDGIGIVLAARRQGNPIPERVTGYDLLLKLLEQGNERTWSFYFLGTDEETSRQAVEK 128 ++VPDG G+V AA G P+ ERVTG+DLL +L+++G +R W+ Y LGT E A EK Sbjct: 65 LIVPDGAGVVWAANYVGTPVTERVTGFDLLHRLMQEGEKRKWTAYLLGTSTEIIEAAAEK 124 Query: 129 ITHSYPKVRIAGRHHGFFDAAEEPEIIAGIQQSRPDILILAMGAPYSDKWLHKHKDELAG 188 + YP+VRI G +G+F ++ E+I ++Q+ PD+L +A GA + W+ K+K +L G Sbjct: 125 LQLQYPQVRIVGCRNGYFGPEQDAEVIEAVRQAAPDLLFVARGADTQEPWIAKYKKQL-G 183 Query: 189 VKLVFGVGGSLDVISGKVKPTPPVWKKLNLEWAHRLLFSPVAKGQKSRWRRQSALPKFVY 248 V + GVGGS D+I+GK+K P +++KL LEW +RLL P R R LPKFV Sbjct: 184 VPFIMGVGGSFDIIAGKLKRAPVLFQKLRLEWFYRLLQEP------KRLPRMMVLPKFVV 237 Query: 249 RTM 251 + + Sbjct: 238 KVV 240 >C0Z749.1 C0Z749_BREBN Probable teichoic acid biosynthesis protein A EC=2.4.1.187 Length = 245 Score = 188 bits (477), Expect = 4e-46, Method: Compositional matrix adjust. Identities = 101/237 (42%), Positives = 144/237 (60%), Gaps = 12/237 (5%) Query: 13 SILGVRFSKLTLEETVLHLAEQIENKRDKLFHLITANPEIXXXX--XXLLQRIIQSADMV 70 +IL V F+ ETV H+ E+I+ + H++TANPEI + I++ A V Sbjct: 7 TILDVPFTTRGFRETVDHITERIQGGQKT--HVVTANPEIVMVARENRAFRSIVEQA-YV 63 Query: 71 VPDGIGIVLAARRQGNPIPERVTGYDLLLKLLEQGNERTWSFYFLGTDEETSRQAVEKIT 130 VPDGIGIV AA+ PI ERVTG +LL L+ + ++ W Y LG + A +K++ Sbjct: 64 VPDGIGIVYAAKWTNQPIYERVTGVELLEALMAKADQHQWGVYLLGAKPDVIHLAKDKLS 123 Query: 131 HSYPKVRIAGRHHGFFDAAEEPEIIAGIQQSRPDILILAMGAPYSDKWLHKHKDELAGVK 190 YP RI G G+F EE +I+ I +++P +L +A+GAP D+W+ K++D+L Sbjct: 124 ARYPNARIVGCRDGYFRPEEETQIVQEIAEAKPQLLFVALGAPRQDEWMAKYRDQL-NAS 182 Query: 191 LVFGVGGSLDVISGKVKPTPPVWKKLNLEWAHRLLFSPVAKGQKSRWRRQSALPKFV 247 L+ GVGGS DVISGKVK P +W+KL+LEW +RL P SRW+RQ A+P+FV Sbjct: 183 LMMGVGGSFDVISGKVKRAPEIWQKLHLEWFYRLASEP------SRWKRQLAIPRFV 233 >B3K7I1.1 B3K7I1_9BACI Glycosyl transferase, WecB/TagA/CpsF family EC=2.4.1.187 Length = 252 Score = 185 bits (470), Expect = 3e-45, Method: Compositional matrix adjust. Identities = 101/243 (41%), Positives = 150/243 (61%), Gaps = 11/243 (4%) Query: 11 TVSILGVRFSKLTLEETVLHLAEQIENKRDKLFHLITANPEIXXXXXX--LLQRIIQSAD 68 TVS+ + FSKL++++TV +L E +++ + H+ITANP + +R++Q A+ Sbjct: 9 TVSVFDIPFSKLSMKDTVKYLTEAVQSGQPH--HVITANPIMVMTAVNNPEYKRMMQGAE 66 Query: 69 MVVPDGIGIVLAARRQGNPIPERVTGYDLLLKLLEQGNERTWSFYFLGTDEETSRQAVEK 128 ++VPDG GIV AA G+P+ RVTG++LL +LL+ G W F+ LGT E ++A E+ Sbjct: 67 IIVPDGTGIVWAAGVGGDPLEGRVTGFELLHELLKVGETYRWKFFLLGTTAEVIQEAAER 126 Query: 129 ITHSYPKVRIAGRHHGFFDAAEEPEIIAGIQQSRPDILILAMGAPYSDKWLHKHKDELAG 188 + YP I G GFF E+ +I I+ + PDIL +A GA + W+ +HK L Sbjct: 127 LQMQYPAAIICGYRDGFFGPDEDAAVIEQIRAASPDILFVARGADNQEPWIAQHKQALQ- 185 Query: 189 VKLVFGVGGSLDVISGKVKPTPPVWKKLNLEWAHRLLFSPVAKGQKSRWRRQSALPKFVY 248 V +V GVGGS D+ISGK+K P V++KL LEW +RLL P +R++R ALPKFV Sbjct: 186 VPVVMGVGGSFDIISGKLKRAPKVFQKLRLEWFYRLLKEP------TRYKRMLALPKFVV 239 Query: 249 RTM 251 + + Sbjct: 240 KVL 242 >A4J962.1 A4J962_DESRM N-acetylmannosaminyltransferase EC=2.4.1.187 Length = 243 Score = 179 bits (454), Expect = 2e-43, Method: Compositional matrix adjust. Identities = 96/239 (40%), Positives = 137/239 (57%), Gaps = 10/239 (4%) Query: 12 VSILGVRFSKLTLEETVLHLAEQIENKRDKLFHLITANPEIX--XXXXXLLQRIIQSADM 69 + +LG L ++ETV + E + K K +IT NPE L +++Q AD+ Sbjct: 3 IKLLGAPVDALNMKETVSQIKEFFK-KGSKPHFIITLNPEYLYRAQDNEELMKLVQGADL 61 Query: 70 VVPDGIGIVLAARRQGNPIPERVTGYDLLLKLLEQGNERTWSFYFLGTDEETSRQAVEKI 129 V PDG GIV AA+ G P+PERVTG DL+L L+ + W + LG + + + Sbjct: 62 VTPDGTGIVWAAKMAGFPVPERVTGIDLMLNLIPVAEQEGWGIFLLGAAPGVAEDTAKNL 121 Query: 130 THSYPKVRIAGRHHGFFDAAEEPEIIAGIQQSRPDILILAMGAPYSDKWLHKHKDELAGV 189 YP + IAG H G+F EE I+ I +++P +L +A+G P ++W+H++KD+L GV Sbjct: 122 KKQYPNLIIAGTHDGYFKPQEEAAIVKKIAEAKPHLLFVALGMPRQEQWIHRYKDQL-GV 180 Query: 190 KLVFGVGGSLDVISGKVKPTPPVWKKLNLEWAHRLLFSPVAKGQKSRWRRQSALPKFVY 248 + GVGGS DVI+G+V+ P +KLNLEW RLL P RWRRQ LPKF + Sbjct: 181 PVSMGVGGSFDVIAGRVERVSPWLQKLNLEWLGRLLKEP------QRWRRQLVLPKFAW 233 >C3H960.1 C3H960_BACTU Glycosyl transferase, WecB/TagA/CpsF Length = 246 Score = 178 bits (451), Expect = 5e-43, Method: Compositional matrix adjust. Identities = 96/245 (39%), Positives = 147/245 (60%), Gaps = 11/245 (4%) Query: 11 TVSILGVRFSKLTLEETVLHLAEQIENKRDKLFHLITANPEIXXXXXX--LLQRIIQSAD 68 TV ILGV FS +T++ETV +L EQ+E++R F ++TANPEI + + + D Sbjct: 5 TVDILGVPFSTMTMDETVQYLKEQLESERTHTFQVVTANPEIVMCAKKDEKFYQTLLNTD 64 Query: 69 MVVPDGIGIVLAARRQGNPIPERVTGYDLLLKLLEQGNE--RTWSFYFLGTDEETSRQAV 126 ++ PDGIG+V A+ G P+ ERV G+DL+ LL + +E + S + LG + A Sbjct: 65 LITPDGIGVVKASGMLGTPLKERVAGFDLMCNLLAKLSEDSKPVSVFLLGAKPHVVQAAA 124 Query: 127 EKITHSYPKVRIAGRHHGFFDAAEEPEIIAGIQQSRPDILILAMGAPYSDKWLHKHKDEL 186 E +T +Y V IAG G+F +E II+ IQ+++PD+L++A+G P + ++ +K L Sbjct: 125 EHLTKTYSAVSIAGIQDGYFKQEDEENIISRIQEAKPDLLLVALGFPRQENFIQNNKHRL 184 Query: 187 AGVKLVFGVGGSLDVISGKVKPTPPVWKKLNLEWAHRLLFSPVAKGQKSRWRRQSALPKF 246 K+ GVGGSLDV +G+VK P + ++LEW +RL +P +RWRRQ L +F Sbjct: 185 E-TKMAVGVGGSLDVWAGEVKRAPKWIQAIHLEWFYRLCSNP------TRWRRQLVLAEF 237 Query: 247 VYRTM 251 + M Sbjct: 238 LKEVM 242 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.320 0.138 0.413 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,572,429,851 Number of extensions: 66323771 Number of successful extensions: 147052 Number of sequences better than 1.0e-05: 856 Number of HSP's gapped: 145302 Number of HSP's successfully gapped: 856 Length of query: 254 Length of database: 3,060,696,203 Length adjustment: 133 Effective length of query: 121 Effective length of database: 1,807,701,208 Effective search space: 218731846168 Effective search space used: 218731846168 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 129 (54.3 bits)