BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0311.0004.p (472 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value C0Z7K3.1 C0Z7K3_BREBN Two-component sensor histidine kinase EC=2... 302 5e-80 C0Z912.1 C0Z912_BREBN Two-component sensor histidine kinase EC=2... 292 4e-77 Q8ENG9.1 Q8ENG9_OCEIH Sensor protein EC=2.7.13.3 259 5e-67 C0Z8I0.1 C0Z8I0_BREBN Two-component sensor histidine kinase EC=2... 208 8e-52 C1TI38.1 C1TI38_9FIRM Signal transduction histidine kinase, COG0642 203 4e-50 >C0Z7K3.1 C0Z7K3_BREBN Two-component sensor histidine kinase EC=2.7.3.- Length = 484 Score = 302 bits (774), Expect = 5e-80, Method: Compositional matrix adjust. Identities = 172/472 (36%), Positives = 265/472 (56%), Gaps = 15/472 (3%) Query: 9 KRKKHTSILSYWTVRYXXXXXXXXXXXALVTFWWIQQEAMSNRMQTTALLAQEIADRSVS 68 K + S+L YWT RY V+ +WI+ A+ +++ T L A+E+ADR V Sbjct: 9 KNNEPVSLLRYWTTRYLLTLVIGLLIIGAVSAYWIKYAAIEKQIEVTRLFAEEVADRVVD 68 Query: 69 ADGSLNVTPALHQLIEDRKRFFKLTQEMCVIITGPKGELLFSQPEMTEKEMR------YE 122 G++ V LHQ++++R++F + + + + I KG++++S+P ++ + Sbjct: 69 DVGNILVGEKLHQVLDNRRKFMGVDRNLILFIEDVKGKVVYSRPTKLPPPLQQAAPELLK 128 Query: 123 LNDSLDAARSPG---FKAAGARIQGNGETLGQVMVLQSKRSLRYIPREEXXXXXXXXXXX 179 D D R P ++ N +G+V++L + ++ + +EE Sbjct: 129 QADMRDKVRHPSGETLYVIRKNLEFNDRIIGEVILLFPETDIK-VGKEEIQYLVIMLGSL 187 Query: 180 XXXSWLTIYLLSIKLAKPIQRVASAASQISGGKYNIILETEAKEREIHELLLSFQEMAGK 239 W IY + KL++PIQ V +A QI G Y+++L KE+EIHEL+ +F EMA + Sbjct: 188 GVLGWAVIYFHARKLSEPIQNVVMSARQIVEGNYDVLLNKTIKEKEIHELVHTFAEMADR 247 Query: 240 LQQFEQSRAVMLAGVSHELKTPVTSIKGLVHAVREGIVEGEEAGEFLDIALLEAGRLQRM 299 L+Q E R +LAGV+HELKTPVTSI GL+ A+ + +V GEE EFL+I L E RLQ+M Sbjct: 248 LRQLESMRTELLAGVTHELKTPVTSISGLIQAINDEVVSGEEQKEFLEICLKETKRLQKM 307 Query: 300 VADLLDYNALAAGIVAIRHDRLEAVPLLEEIVYQWKLTQD-GEVCTPELQLPRQPLFLRG 358 V DLLD+N+ A G + + + L+ EI QW++ D G V L +Q + + Sbjct: 308 VEDLLDFNSFAVGAITVCQEEQNVNKLVREIASQWQIVNDLGHVTFTVGVLDKQTM-IYV 366 Query: 359 DPLRIQQILVNLLNNSVQAKLPGQMLQLDLRLEENPQGYAEITVTDNGTGIPEENRERIF 418 DP R+QQIL+NLLNN+ QA G + L L E I V D+G GIP + + +F Sbjct: 367 DPGRVQQILINLLNNAKQA--VGGKGSIALTLYETDTDL-RIDVKDDGPGIPAKEQPLVF 423 Query: 419 EAFFRSSGKQSTLRGLGLGLTFSRLLAEAMGGSLRLGDGPEQGCTFVIPLPL 470 E FFR S K+ +RGLGLGL++SR++A+A+GG L L + +G F + LP+ Sbjct: 424 ERFFRGSNKKDKVRGLGLGLSYSRMMAKALGGDLLLANSTSEGSVFTLILPM 475 >C0Z912.1 C0Z912_BREBN Two-component sensor histidine kinase EC=2.7.3.- Length = 477 Score = 292 bits (748), Expect = 4e-77, Method: Compositional matrix adjust. Identities = 178/470 (37%), Positives = 257/470 (54%), Gaps = 19/470 (4%) Query: 11 KKHTSILSYWTVRYXXXXXXXXXXXALVTFWWIQQEAMSNRMQTTALLAQEIADRSVSAD 70 KK TS+ YWT RY + + +WI R+ L+A E+ADR V + Sbjct: 13 KKRTSLFRYWTTRYLIILCIGLFVIGIASSYWISYSETQKRLDFMRLMAAEVADRVVDME 72 Query: 71 GSLNVTPALHQLIEDRKRFFKLTQEMCVIITGPKGELLFSQPEMTEKEMR------YELN 124 G + + P L ++++ R+ + + ++I + +F P E++ E N Sbjct: 73 GKVKMAPFLFRIVDSRQESLGVNYKPMMMILDEHKQPVFGVPGPFSSELKRLAPDLVEAN 132 Query: 125 DSL---DAARSPGFKAAGARIQGNGETLGQVMVLQSKRSLRYIPREEXXXXXXXXXXXXX 181 DSL + AR I+ + +G VM+ ++ L E Sbjct: 133 DSLSQFELARGDEVLFVKESIKVDERVVGWVMLFTPQKQL-IRSMTEFQLLAIMLLGLGL 191 Query: 182 XSWLTIYLLSIKLAKPIQRVASAASQISGGKYNIILETEAKEREIHELLLSFQEMAGKLQ 241 WL IYLL+ KL++PI+ VA AA QI G Y I L+ +E+E++EL+ SF+EMA +L+ Sbjct: 192 LGWLVIYLLTKKLSQPIKDVADAAKQIVMGNYEIQLDKNKREQEVYELIHSFEEMAERLK 251 Query: 242 QFEQSRAVMLAGVSHELKTPVTSIKGLVHAVREGIVEGEEAGEFLDIALLEAGRLQRMVA 301 Q E R +LAGV+HELKTPVTSI GLV AVRE +V GEEA EFLDI E RL +MV Sbjct: 252 QLEMMRTELLAGVTHELKTPVTSISGLVQAVREEVVSGEEAKEFLDICTKETTRLIKMVE 311 Query: 302 DLLDYNALAAGIVAIRHDRLEAVPLLEEIVYQWKLTQDGEVCTPELQLPRQPLFLRGDPL 361 DLLD+N+ A G + IR L+ EI +QW++ Q+ E + ++ P+ + DPL Sbjct: 312 DLLDFNSFAVGDIRIRRQPQNMQELIREITHQWRILQEEEKLSLHIESTPDPIMIDTDPL 371 Query: 362 RIQQILVNLLNNSVQAKLPGQMLQLDLRLEENPQGYAE---ITVTDNGTGIPEENRERIF 418 R+QQI+ NLLNN+ QA ++ + R+ + AE I V DNG GIP E + IF Sbjct: 372 RVQQIMYNLLNNAAQA------MESEGRIVVSLSASAEEIRIDVKDNGRGIPIEEQSLIF 425 Query: 419 EAFFRSSGKQSTLRGLGLGLTFSRLLAEAMGGSLRLGDGPEQGCTFVIPL 468 E F+R K+ +RGLGLGL+FSR++A+A+GG L L + G TF + L Sbjct: 426 ERFYRGEDKKHIVRGLGLGLSFSRMIAQALGGILILTESTASGSTFTLIL 475 >Q8ENG9.1 Q8ENG9_OCEIH Sensor protein EC=2.7.13.3 Length = 485 Score = 259 bits (661), Expect = 5e-67, Method: Compositional matrix adjust. Identities = 157/472 (33%), Positives = 251/472 (53%), Gaps = 16/472 (3%) Query: 9 KRKKHTSILSYWTVRYXXXXXXXXXXXALVTFWWIQQEAMSNRMQTTALLAQEIADRSVS 68 + K + YWT RY A+++ WWI+ + NR+Q LL +++ADR Sbjct: 11 NKSKRIPMRRYWTSRYLITLCVGLLIIAILSVWWIRHSTLENRVQMMELLTEDMADRYTE 70 Query: 69 ADGSLNVTPALHQLIEDRKRFFKLTQEMCVIITGPKGELLFSQPEMT--EKEMRYELNDS 126 + G + D +R+ L QE + I GE++ M+ ++E+ E+ DS Sbjct: 71 SRGERPQPGGIPGGFPD-QRWMGLEQEPLIYIIDRSGEIISRNRPMSPLDQEIDIEMLDS 129 Query: 127 --------LDAARSPGFKAAGARIQGNGETLGQVMVLQSKRSLRYIPREEXXXXXXXXXX 178 +D F + I + T+G VM+ +SK+ L + E Sbjct: 130 TETVQTLKMDDQNRLTFYMVKSPIVMDDTTIGWVMMAESKQLLTKV-NHEYKLLATMIIS 188 Query: 179 XXXXSWLTIYLLSIKLAKPIQRVASAASQISGGKYNIILETEAKEREIHELLLSFQEMAG 238 W IY+L+ +L+ PI++VA+AA I G Y + L +KE EIHEL+ SF+ MA Sbjct: 189 LALLGWAAIYILTKRLSDPIKQVANAARNIEEGDYKVELSNNSKEEEIHELVSSFKGMAN 248 Query: 239 KLQQFEQSRAVMLAGVSHELKTPVTSIKGLVHAVREGIVEGEEAGEFLDIALLEAGRLQR 298 +L++ E R +LAGV+HELKTPVTSI GL+ A+ + +V EE EFL+I+L E ++Q Sbjct: 249 RLERLEAIRTELLAGVTHELKTPVTSISGLLQAINDDVVNDEERKEFLEISLKETEKMQT 308 Query: 299 MVADLLDYNALAAGIVAIRHDRLEAVPLLEEIVYQWKLTQDGEVCTPELQLPRQPLFLRG 358 M+ DLL +N+ A+ + + +++ +EEIV+ W++T E++ + + Sbjct: 309 MIEDLLAFNSFASSTLPLNREKVAINETIEEIVHDWEITNLNNSIRVEVERNSEEAIVEV 368 Query: 359 DPLRIQQILVNLLNNSVQAKLPGQMLQLDLRLEENPQGYAEITVTDNGTGIPEENRERIF 418 D +R++QI+ NL NN+ QA G ++ + + +E Q I V D G GIP E + IF Sbjct: 369 DWIRLEQIITNLFNNASQAMSEG-IITVKILIE---QQDVWIYVVDGGIGIPMEEQALIF 424 Query: 419 EAFFRSSGKQSTLRGLGLGLTFSRLLAEAMGGSLRLGDGPEQGCTFVIPLPL 470 E F+R + K+ + RGLGLGL S+++A+AM G L L + G T + LP+ Sbjct: 425 ERFYRGNEKKYSTRGLGLGLALSKMIAQAMNGDLTLLHSDKSGTTMKLQLPI 476 >C0Z8I0.1 C0Z8I0_BREBN Two-component sensor histidine kinase EC=2.7.3.- Length = 518 Score = 208 bits (530), Expect = 8e-52, Method: Compositional matrix adjust. Identities = 119/287 (41%), Positives = 174/287 (60%), Gaps = 7/287 (2%) Query: 184 WLTIYLLSIKLAKPIQRVASAASQISGGKYNIIL--ETEAKEREIHELLLSFQEMAGKLQ 241 W +Y+LS L +P++++A AA QIS G Y L ++ KE EI +L+ SF EMA +L Sbjct: 230 WAIVYVLSRSLTQPLRQLAIAAEQISSGNYTPSLPNSSKIKEAEISQLVHSFDEMAKRLG 289 Query: 242 QFEQSRAVMLAGVSHELKTPVTSIKGLVHAVREGIVEGEEAGEFLDIALLEAGRLQRMVA 301 Q E+ R +LAGVSHEL+TPVTSI+G++ AV++G+V+G +A EF+ I++ EA RLQ MV Sbjct: 290 QLERMRTDLLAGVSHELRTPVTSIRGMIQAVKDGVVKGADADEFMQISMDEAKRLQTMVN 349 Query: 302 DLLDYNALAAGIVAIRHDRLEAVPLLEEIVYQWKLTQDGEVCTPELQLPRQPLFLRGDPL 361 DLLD++++ AG V ++ L++IV Q + + + Q GD Sbjct: 350 DLLDFSSMEAGEVFAEKQSIQIDSTLDQIVAQVQALPSFANVSVTVNPTGQDTVWIGDES 409 Query: 362 RIQQILVNLLNNSVQAKLPGQMLQLDLRLEENPQGYAEITVTDNGTGIPEENRERIFEAF 421 ++QIL+NLL NS A Q+++ + NP + I +TDNG GIPE IFE + Sbjct: 410 HVKQILLNLLGNSAAAN----ATQINIGV-HNPSDFLSIDITDNGKGIPESEVPFIFERY 464 Query: 422 FRSSGKQSTLRGLGLGLTFSRLLAEAMGGSLRLGDGPEQGCTFVIPL 468 +R K+ +GLGLGLT SRLLA+ GG++ L +G TF + L Sbjct: 465 YRGDSKRKKKQGLGLGLTISRLLAKTHGGNVELVQTSPEGTTFRLTL 511 >C1TI38.1 C1TI38_9FIRM Signal transduction histidine kinase, COG0642 Length = 301 Score = 203 bits (516), Expect = 4e-50, Method: Compositional matrix adjust. Identities = 122/284 (42%), Positives = 173/284 (60%), Gaps = 6/284 (2%) Query: 188 YLLSIKLAKPIQR-VASAASQISGGKYNIILETEAKEREIHELLLSFQEMAGKLQQFEQS 246 Y+ SI A P A AA I+ G Y L KERE+ +L+ SF +MA +L+Q E+ Sbjct: 23 YVFSISKAYPSSAGNAGAAQAIASGHYEPRLPQNLKERELQQLVTSFSDMAFQLKQLERM 82 Query: 247 RAVMLAGVSHELKTPVTSIKGLVHAVREGIVEGEEAGEFLDIALLEAGRLQRMVADLLDY 306 RA +LAGVSHEL+TPVTSI+G++ AV +V G EA EFL I+L E+ RLQ+MV DLLD+ Sbjct: 83 RADLLAGVSHELRTPVTSIRGMIQAVHGKVVTGPEADEFLYISLNESIRLQQMVEDLLDF 142 Query: 307 NALAAGIVAIRHDRLEAVPLLEEIVYQWKLTQDGEVCTPELQLPRQPLFLRGDPLRIQQI 366 + AG I + + LL+E++ Q + E + + + Q +++ GD R++Q+ Sbjct: 143 STFEAGAEPIEKEYFNLLGLLDEVICQVSILAGYEQISFDRKGLDQEVWMTGDRGRLRQV 202 Query: 367 LVNLLNNSVQAKLPGQMLQLDLRLEENPQGYAEITVTDNGTGIPEENRERIFEAFFRSSG 426 +NL +NS +A ++++ LR+ E+ EI V DNG GI E +RE IFE F+R G Sbjct: 203 FLNLFSNSQKAS--ATVIKIVLRVGEDN---IEIDVEDNGKGIEEADREYIFERFYRGQG 257 Query: 427 KQSTLRGLGLGLTFSRLLAEAMGGSLRLGDGPEQGCTFVIPLPL 470 K+ RGLGLGLT SR+L+ A GG L L +G F + LPL Sbjct: 258 KKMKPRGLGLGLTVSRMLSRAHGGDLFLLRTSSEGSVFCLTLPL 301 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.135 0.386 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,507,173,754 Number of extensions: 97384201 Number of successful extensions: 365724 Number of sequences better than 1.0e-05: 27516 Number of HSP's gapped: 317938 Number of HSP's successfully gapped: 27605 Length of query: 472 Length of database: 3,060,696,203 Length adjustment: 139 Effective length of query: 333 Effective length of database: 1,751,175,118 Effective search space: 583141314294 Effective search space used: 583141314294 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 133 (55.8 bits)