BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0311.0001.p (500 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value C0ZCL4.1 C0ZCL4_BREBN Putative amidase 520 e-145 Q2B3F0.1 Q2B3F0_9BACI Amidase 511 e-143 A6CQF1.1 A6CQF1_9BACI Amidase EC=3.5.1.4 500 e-139 C2PE53.1 C2PE53_BACCE Glutaminyl-tRNA synthase, glutamine-hydrol... 465 e-129 B2A6I4.1 B2A6I4_NATTJ Amidase 464 e-129 >C0ZCL4.1 C0ZCL4_BREBN Putative amidase Length = 483 Score = 520 bits (1339), Expect = e-145, Method: Compositional matrix adjust. Identities = 259/478 (54%), Positives = 339/478 (70%), Gaps = 2/478 (0%) Query: 20 IVEATIPEIQAALETGQMTSRQLVLMYLERIAEHDKSGLTVNSVLEINPDALFIAEALDV 79 I E +I E QAA+ G TSR+L L +L+RIA ++K G+ +N++ E+NPDAL IAE+LD Sbjct: 4 IHETSILEWQAAMTAGTTTSRELTLAFLQRIATYNKQGIRINAICELNPDALAIAESLDR 63 Query: 80 ERSLQGPRGPLHGIPVLLKDNINTGDKMHTSAGSLALADSFAGEDAFIVTRLRDAGAVIM 139 ER++ G RGPLHGIPVL+KDNI T DKMHT+AG+LALADSFA DAF+VT+LR+AGAVI+ Sbjct: 64 ERAVSGSRGPLHGIPVLIKDNIATSDKMHTTAGALALADSFASADAFVVTKLREAGAVIL 123 Query: 140 GKANMTEFANFMTNGMPSGYSSRGGQVLNPYNIST-PTXXXXXXXXXXXXCNFCTVSVGT 198 GK N+TE+ANF++N MP GYSSRGGQVLNPY F V+VGT Sbjct: 124 GKTNLTEWANFISNDMPDGYSSRGGQVLNPYGPGVLDVGGSSSGSAAGIAAGFAVVAVGT 183 Query: 199 ETSGSILNPGNLGSIIGIKPTVGMLSRSGILPLSNTQDTAGPMARTVRDAVLLLNAMLGK 258 ETSGSIL+P S++GIKPTVG++SRSGI+P+S++QDTAGP+ARTV DA +LL A+ G Sbjct: 184 ETSGSILHPAEQNSLVGIKPTVGLISRSGIIPISHSQDTAGPLARTVTDAAILLGALTGI 243 Query: 259 DDSDAAMGTGVGRLHEDYTVFLDPNGLQGARIGIPRDYYFEELTDEQLALFNASVERMRG 318 D+ D G G DY FLD +GL+GARIG+ R + E E++AL+ A++E+++ Sbjct: 244 DEKDPVTGKSEGLAQTDYLPFLDADGLRGARIGVVRSRFLAECEAEEIALYEAAIEKLKE 303 Query: 319 LGATIIDPADIRTAREIKYSSVVLNEFKTSLNAYLARLGPGAPMRTLKDIIDFNHAHPME 378 GATIID I T V+++EFK +NAYL L P+R+L+D+I FN AH + Sbjct: 304 AGATIIDAVTIPTENAEWNIHVLVHEFKVGVNAYLKTLPASYPIRSLQDVIAFNRAHEEQ 363 Query: 379 TLRYGQSTLLDAEYTSSGTFTEPQYLRDRATDLRLCKELGIDAAMKEHQLDALLFPADFG 438 L YGQ L ++E T SGT TEP+YL + DL + ++ G+DA MKEHQLDALL+P G Sbjct: 364 ALLYGQELLEESEKT-SGTLTEPEYLANLLFDLEMSQKQGLDAVMKEHQLDALLYPGSTG 422 Query: 439 ARITSRAGYPSIVVPSGYTSAGVPFGVTFSARAYQEPVLIRLAYAYEQNYKIRKAPSL 496 I ++AGYPSI VP+GYTSAG PFG+ + A+QEP L+RLAYAYEQ ++R AP + Sbjct: 423 YAIPAKAGYPSITVPAGYTSAGKPFGIMLTGLAFQEPTLLRLAYAYEQATRLRVAPDM 480 >Q2B3F0.1 Q2B3F0_9BACI Amidase Length = 506 Score = 511 bits (1315), Expect = e-143, Method: Compositional matrix adjust. Identities = 254/478 (53%), Positives = 336/478 (70%), Gaps = 2/478 (0%) Query: 20 IVEATIPEIQAALETGQMTSRQLVLMYLERIAEHDKSGLTVNSVLEINPDALFIAEALDV 79 ++EATI E+Q LE G+++S++LVLMYL RIA+ DK+G +NSVLE+NPDA+ IA ALD Sbjct: 30 LLEATIDEMQEKLEKGEVSSKELVLMYLHRIAQKDKNGPAINSVLEVNPDAVQIAAALDA 89 Query: 80 ERSLQGPRGPLHGIPVLLKDNINTGDKMHTSAGSLALADSFAGEDAFIVTRLRDAGAVIM 139 ER L+G RGPLHGIPVL+KDNI+T DKMHTSAGSLAL +S A ED+++ LR AGAVI+ Sbjct: 90 ERKLKGSRGPLHGIPVLIKDNIDTADKMHTSAGSLALKESVAKEDSYVAEALRKAGAVIL 149 Query: 140 GKANMTEFANFMTNGMPSGYSSRGGQVLNPYNIST-PTXXXXXXXXXXXXCNFCTVSVGT 198 GK NMTE+ANFMT GMPSGYSSRGGQVLNPY NF VSVGT Sbjct: 150 GKTNMTEWANFMTEGMPSGYSSRGGQVLNPYGPGNFDVGGSSSGSGAAIASNFAAVSVGT 209 Query: 199 ETSGSILNPGNLGSIIGIKPTVGMLSRSGILPLSNTQDTAGPMARTVRDAVLLLNAMLGK 258 ETSGSIL+P + S++GIKPTVG++SR GI+P++++QDTAGPMARTVRDAV LL+ + G Sbjct: 210 ETSGSILSPASQNSLVGIKPTVGLISRRGIIPIAHSQDTAGPMARTVRDAVYLLDVLAGN 269 Query: 259 DDSDAAMGTGVGRLHEDYTVFLDPNGLQGARIGIPRDYYFEELTDEQLALFNASVERMRG 318 DD D A+ + ++ FLD NGL+G RIGI R+ YF+ L+ ++L + N +VE+++ Sbjct: 270 DDRDPAVQNNPESDYTEFAGFLDENGLKGKRIGIAREVYFDYLSGDKLEVMNHAVEQLKA 329 Query: 319 LGATIIDPADIRTAREIKYSSVVLNEFKTSLNAYLARLGPGAPMRTLKDIIDFNHAHPME 378 LGA ++DP +I + + V+ EFK LNAYL + P +++L D+I FN + Sbjct: 330 LGAEVVDPVEIPSTKNNWKYDVLTYEFKADLNAYLRGVAPHIKVKSLADVIAFNLENSEA 389 Query: 379 TLRYGQSTLLDAEYTSSGTFTEPQYLRDRATDLRLCKELGIDAAMKEHQLDALLFPADFG 438 L+YGQ+ L +AE T SGT TE Y+ D+ E GID +KEH LDA++ P +FG Sbjct: 390 ALKYGQTILEEAERT-SGTLTEEAYISSLEEDVYFSTEEGIDHVLKEHHLDAIVTPNNFG 448 Query: 439 ARITSRAGYPSIVVPSGYTSAGVPFGVTFSARAYQEPVLIRLAYAYEQNYKIRKAPSL 496 A I ++AGYPSI VP+GY+ P G+TF+ A+ E LI +AYA+EQ K+RK+P L Sbjct: 449 AGIPAKAGYPSITVPAGYSLENEPVGITFTGTAFSEAGLISMAYAFEQGTKVRKSPLL 506 >A6CQF1.1 A6CQF1_9BACI Amidase EC=3.5.1.4 Length = 487 Score = 500 bits (1287), Expect = e-139, Method: Compositional matrix adjust. Identities = 255/476 (53%), Positives = 330/476 (69%), Gaps = 3/476 (0%) Query: 20 IVEATIPEIQAALETGQMTSRQLVLMYLERIAEHDKSGLTVNSVLEINPDALFIAEALDV 79 ++EATI E+ L TG++TS LVLMYL RIAE D+SG ++NS++E+NPDAL IA +LD Sbjct: 14 LIEATISELNDKLHTGEITSHDLVLMYLGRIAEVDQSGPSINSLIEVNPDALHIAASLDY 73 Query: 80 ERSLQGPRGPLHGIPVLLKDNINTGDKMHTSAGSLALADSFAGEDAFIVTRLRDAGAVIM 139 ER +G RGPLHGIPV++KDNI+TGD MHTSAGSLALA+S+A EDAF+V +LR+AGA+I+ Sbjct: 74 ERKTKGSRGPLHGIPVVIKDNIDTGDNMHTSAGSLALAESYAKEDAFLVKKLREAGAIIL 133 Query: 140 GKANMTEFANFMTNGMPSGYSSRGGQVLNPYNISTPTXXXXXXXXXXXXCNFCTVSVGTE 199 GK N+TE+ANFM MP+GYSSRGGQVLNPY N VSVGTE Sbjct: 134 GKTNLTEWANFMAEKMPTGYSSRGGQVLNPYGADFMVGGSSAGSAAAIAANLAVVSVGTE 193 Query: 200 TSGSILNPGNLGSIIGIKPTVGMLSRSGILPLSNTQDTAGPMARTVRDAVLLLNAMLGKD 259 TSGSIL+P + S++GIKPTVG++SR+G++P+S+TQDTAGPMARTV DA +LL A+ G D Sbjct: 194 TSGSILSPASQNSLVGIKPTVGLISRTGVIPISHTQDTAGPMARTVTDAAVLLQALQGVD 253 Query: 260 DSDAAMGTGVGRLHEDYTVFLDPNGLQGARIGIPRDYYFEELTDEQLALFNASVERMRGL 319 DA + L DYT L +GLQG RIGI R YF+ L + +LA+ NA+VE++ L Sbjct: 254 KKDAITASN-ELLETDYTGHLLESGLQGKRIGIARSTYFDYLDESKLAIMNAAVEKLAEL 312 Query: 320 GATIIDPADIRTAREIKYSSVVLNEFKTSLNAYLARLGPGAPMRTLKDIIDFNHAHPMET 379 GA ++D +I + +V+L EFK +NAYL + P +R L DII FN Sbjct: 313 GAEVVDSVEIPSTEADWDINVLLYEFKAGINAYLKTVDPSLGIRNLSDIIRFNTETGEPA 372 Query: 380 LRYGQSTLLDAEYTSSGTFTEPQYLRDRATDLRLCKELGIDAAMKEHQLDALLFPADFGA 439 L+YGQ + ++E T +P YLR D L K+ GIDA +KEHQLDA++FP +FGA Sbjct: 373 LKYGQKVMEESEETKG--LIDPLYLRSLEKDQYLSKKNGIDAVLKEHQLDAIVFPNNFGA 430 Query: 440 RITSRAGYPSIVVPSGYTSAGVPFGVTFSARAYQEPVLIRLAYAYEQNYKIRKAPS 495 I ++AGYPSI VP+G+T G P G+TF+ AY EP LI A+AYEQ K RK P+ Sbjct: 431 MIPAKAGYPSITVPAGFTPEGEPVGITFTGGAYSEPSLIEAAFAYEQGTKHRKPPA 486 >C2PE53.1 C2PE53_BACCE Glutaminyl-tRNA synthase, glutamine-hydrolyzing, subunit A (Glutamyl-tRNA(Gln) amidotransferase, subunit A) Length = 491 Score = 465 bits (1197), Expect = e-129, Method: Compositional matrix adjust. Identities = 237/482 (49%), Positives = 321/482 (66%), Gaps = 10/482 (2%) Query: 22 EATIPEIQAALETGQMTSRQLVLMYLERIAEHDKSGLTVNSVLEINPDALFIAEALDVER 81 E TI +IQ A+E GQ+TS++LV+ YL RIA++D+ G +NS+LEINPDA+FIAEALD ER Sbjct: 12 ELTIHDIQVAMEAGQLTSKELVMYYLHRIAKYDQEGPKINSILEINPDAIFIAEALDYER 71 Query: 82 SLQGPRGPLHGIPVLLKDNINTGDKMHTSAGSLALADSFAGEDAFIVTRLRDAGAVIMGK 141 +G RGPLHGIPVLLKDNI T D MHTSAG++AL + + EDAF+VT+LR+AGAVI+GK Sbjct: 72 KTKGGRGPLHGIPVLLKDNIETNDSMHTSAGTIALEQNISSEDAFLVTKLREAGAVIIGK 131 Query: 142 ANMTEFANFMTNGMPSGYSSRGGQVLNPYNISTP---TXXXXXXXXXXXXCNFCTVSVGT 198 NMTE AN M+ M +GYS+RGGQ +NPY NF VSVGT Sbjct: 132 TNMTELANGMSFDMWAGYSARGGQTINPYGTGEDDMFVGGSSTGSAIAVAANFTVVSVGT 191 Query: 199 ETSGSILNPGNLGSIIGIKPTVGMLSRSGILPLSNTQDTAGPMARTVRDAVLLLNAMLGK 258 ET GSIL+P S++GIKPTVG++SR GI+PL+ +QDTAGP ARTV DA +LL ++ G Sbjct: 192 ETDGSILSPAVQNSVVGIKPTVGLISRRGIIPLTYSQDTAGPFARTVTDAAILLGSLTGL 251 Query: 259 DDSDAAMGTGVGRLHEDYTVFLDPNGLQGARIGI----PRDYYFEELTDEQLALFNASVE 314 D+ D A G+ DYT +LD NGL GA+IG+ P+DYY DE+ LF ++E Sbjct: 252 DEKDVATHKSEGKAEHDYTKYLDDNGLNGAKIGVYSNAPKDYYETGEYDEK--LFKETIE 309 Query: 315 RMRGLGATIIDPADIRTAREIKYSSVVLNEFKTSLNAYLARLGPGAPMRTLKDIIDFNHA 374 +R GAT+++ DI + V L E K SL+ YL++L P+ ++ ++++FN Sbjct: 310 VLRSEGATVVEDIDIPSFHREWSWGVSLYELKHSLDNYLSKLPSTIPVHSISELMEFNKN 369 Query: 375 HPMETLRYGQSTLLDAEYTSSGTFTEPQYLRDRATDLRLCKELGIDAAMKEHQLDALLFP 434 L+YGQ T L+ T P+YL R D+ +E GID A++++ LDA+LFP Sbjct: 370 IAERALKYGQ-TKLEIRKDFPNTLRNPEYLNARLEDIYFSQEQGIDFALEKYNLDAILFP 428 Query: 435 ADFGARITSRAGYPSIVVPSGYTSAGVPFGVTFSARAYQEPVLIRLAYAYEQNYKIRKAP 494 + G+ I+++AGYPSI +P+GY G PFG+T ++ A+ E LI+LAYA+EQ K RK P Sbjct: 429 SYIGSTISAKAGYPSIAIPAGYMDNGRPFGITLASTAFSEGTLIKLAYAFEQATKHRKIP 488 Query: 495 SL 496 +L Sbjct: 489 NL 490 >B2A6I4.1 B2A6I4_NATTJ Amidase Length = 488 Score = 464 bits (1195), Expect = e-129, Method: Compositional matrix adjust. Identities = 239/478 (50%), Positives = 327/478 (68%), Gaps = 6/478 (1%) Query: 20 IVEATIPEIQAALETGQMTSRQLVLMYLERIAEHDKSGLTVNSVLEINPDALFIAEALDV 79 ++E T+ E+Q +E G++TSR LVLMY RIA D+ G +NS++E+NP+AL IA+ALD Sbjct: 14 LLETTVDELQDYMEKGEITSRDLVLMYQNRIACLDQDGPKINSIIEMNPEALQIAQALDR 73 Query: 80 ERSLQGPRGPLHGIPVLLKDNINTGDKMHTSAGSLALADSFAGEDAFIVTRLRDAGAVIM 139 ER +GPR LHGIPVLLKDNI+T DKM TSAGS+AL + A EDAFIV +LR AGAVI+ Sbjct: 74 ERKYKGPRSKLHGIPVLLKDNIDTEDKMRTSAGSIALEEHVAREDAFIVKKLRQAGAVIL 133 Query: 140 GKANMTEFANFMTNGMPSGYSSRGGQVLNPYNI-STPTXXXXXXXXXXXXCNFCTVSVGT 198 GKAN+TE+ANFM + MP+GYSSRGGQVLNPY NF +++GT Sbjct: 134 GKANLTEWANFMADNMPNGYSSRGGQVLNPYGPGELDCGGSSSGSAAALAANFTVLAIGT 193 Query: 199 ETSGSILNPGNLGSIIGIKPTVGMLSRSGILPLSNTQDTAGPMARTVRDAVLLLNAMLGK 258 ETSGSIL+P + S++GIKPTVG+ SRSGI+P+S++QDTAGPMARTV DA +LL + G Sbjct: 194 ETSGSILSPASDNSVVGIKPTVGLWSRSGIIPISHSQDTAGPMARTVEDAAILLEILSGP 253 Query: 259 DDSDAAMGTGVGRLHEDYTVFLDPNGLQGARIGIPRDYYFEELTDEQLALFNASVERMRG 318 D D T + YT +L+ +GL+G R+G+ ++ + L D +L + +++ + Sbjct: 254 DAKDPVTLTKKDDIDGRYTNYLNAHGLKGTRLGVSQE-FLNLLEDSELDVIEKAIKNLEN 312 Query: 319 LGATIIDPADIRTAREIKYS-SVVLNEFKTSLNAYLARLGPGAPMRTLKDIIDFNHAHPM 377 LGA I+ + I +I + +V+L+EFK +N YL G P+++L ++I+FN+ + Sbjct: 313 LGAHIV--SGITYPPKIDWDLNVLLHEFKVGINTYLRNTGDRVPVKSLTELINFNYRNKE 370 Query: 378 ETLRYGQSTLLDAEYTSSGTFTEPQYLRDRATDLRLCKELGIDAAMKEHQLDALLFPADF 437 L+YGQ LL + T GT TEP+YL+ R DL K+ GIDA M+E+ LDAL+FPA+ Sbjct: 371 IALKYGQERLLKSNKT-KGTLTEPEYLKSREHDLFYSKDKGIDAVMEEYNLDALIFPANL 429 Query: 438 GARITSRAGYPSIVVPSGYTSAGVPFGVTFSARAYQEPVLIRLAYAYEQNYKIRKAPS 495 GA I +RAGYPSI VP+GY S G P G+TF+A+AY+E LI++AY+YEQ R P Sbjct: 430 GANIPARAGYPSITVPAGYNSTGKPIGLTFTAKAYEEGKLIKIAYSYEQAVSNRVPPQ 487 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.318 0.135 0.384 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,862,881,651 Number of extensions: 120198253 Number of successful extensions: 281076 Number of sequences better than 1.0e-05: 4057 Number of HSP's gapped: 271623 Number of HSP's successfully gapped: 4095 Length of query: 500 Length of database: 3,060,696,203 Length adjustment: 140 Effective length of query: 360 Effective length of database: 1,741,754,103 Effective search space: 627031477080 Effective search space used: 627031477080 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 133 (55.8 bits)