BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0307.0087.p (179 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K5Y6.1 B3K5Y6_9BACI Binding-protein-dependent transport system... 162 1e-38 A8VZX7.1 A8VZX7_9BACI Anti-sigma-factor antagonist and sulfate t... 157 4e-37 B1D6F3.1 B1D6F3_9BACL Binding-protein-dependent transport system... 143 8e-33 B1DBH8.1 B1DBH8_9BACL Binding-protein-dependent transport system... 122 2e-26 B3KA94.1 B3KA94_9BACI Binding-protein-dependent transport system... 112 2e-23 >B3K5Y6.1 B3K5Y6_9BACI Binding-protein-dependent transport systems inner membrane component Length = 306 Score = 162 bits (410), Expect = 1e-38, Method: Compositional matrix adjust. Identities = 74/160 (46%), Positives = 108/160 (67%), Gaps = 2/160 (1%) Query: 8 VEKTVPK--RTYWTRKKREQWAGWIFIAPEVXXXXXXXXXXXXXXXXXXXTEWNLVGGLS 65 V++ VPK R +WT K++E GW+F+APE+ ++W+++ G+ Sbjct: 4 VQQPVPKSRRKFWTPKRKEALIGWLFLAPEIVGMLLLNVFALGFSLYLSLSDWDMLSGVQ 63 Query: 66 AIHFIGLDNFVNLFKDNHFILALKNNVLFTVGTVPLTMLLGIVLSALIHKKLYGKAFFKV 125 I F GLDN++ +F D I A+KNN+++ V TVP+ + + +VLS LIH ++ K++FKV Sbjct: 64 GIEFTGLDNYIKMFHDPTIIQAIKNNLIYMVLTVPIPIAIALVLSVLIHNSVFFKSYFKV 123 Query: 126 AFFIPYICSTVAIAAVWQALYHPSKGPLNQMLMEMGISSP 165 AFFIPYI S +A+AAVW AL+HPS GP+NQ LME+GISSP Sbjct: 124 AFFIPYISSIIAVAAVWSALFHPSLGPINQFLMELGISSP 163 >A8VZX7.1 A8VZX7_9BACI Anti-sigma-factor antagonist and sulfate transporter Length = 327 Score = 157 bits (397), Expect = 4e-37, Method: Compositional matrix adjust. Identities = 76/171 (44%), Positives = 107/171 (62%), Gaps = 6/171 (3%) Query: 9 EKTVPKRTYWTRKKR-EQWAGWIFIAPEVXXXXXXXXXXXXXXXXXXXTEWNLVGGLSAI 67 E PK+ +R KR E GW+F+ PE+ +EWNL+GG S I Sbjct: 23 EAPPPKKASRSRMKRHEAMMGWLFLLPEMIGLAFLYVFPLGFSLFLSFSEWNLIGGFSEI 82 Query: 68 HFIGLDNFVNLFKDNHFILALKNNVLFTVGTVPLTMLLGIVLSALIHKKLYGKAFFKVAF 127 FIGLDNFV +F+D AL+NN+ +T VP++M++ ++L+ LIH K+Y + +FKVAF Sbjct: 83 RFIGLDNFVQMFQDPRVWGALRNNIFYTFMVVPISMMIALLLAVLIHNKVYLRDYFKVAF 142 Query: 128 FIPYICSTVAIAAVWQALYHPSKGPLNQMLMEMGISSPXXXRLIGWLTRAT 178 FIPYI S +A+ AVW+ALYHP++GP+NQ LM +GI +P GWL + Sbjct: 143 FIPYISSIIAVGAVWRALYHPTQGPINQFLMSIGIENPP-----GWLADTS 188 >B1D6F3.1 B1D6F3_9BACL Binding-protein-dependent transport systems inner membrane component Length = 310 Score = 143 bits (360), Expect = 8e-33, Method: Compositional matrix adjust. Identities = 65/152 (42%), Positives = 99/152 (65%) Query: 14 KRTYWTRKKREQWAGWIFIAPEVXXXXXXXXXXXXXXXXXXXTEWNLVGGLSAIHFIGLD 73 ++ +WT +RE GW+F+ PE+ ++W+++ GL I +IGL Sbjct: 17 RKKFWTPSRREALVGWLFLIPEIVGMLLLNVFALGFSLYLSFSKWDMLSGLPGIEWIGLG 76 Query: 74 NFVNLFKDNHFILALKNNVLFTVGTVPLTMLLGIVLSALIHKKLYGKAFFKVAFFIPYIC 133 N+ LF D + ALKNN+L+TV TVP+ + + +VL+ +I+ ++ K++FKV FFIPYI Sbjct: 77 NYKKLFHDPAILEALKNNLLYTVMTVPIPIAIALVLAVVINNSVFLKSYFKVVFFIPYIS 136 Query: 134 STVAIAAVWQALYHPSKGPLNQMLMEMGISSP 165 S +AIAAVW AL HPS GP+NQ+LM++GI +P Sbjct: 137 SIIAIAAVWSALLHPSLGPINQLLMQLGIDAP 168 >B1DBH8.1 B1DBH8_9BACL Binding-protein-dependent transport systems inner membrane component Length = 310 Score = 122 bits (305), Expect = 2e-26, Method: Compositional matrix adjust. Identities = 59/153 (38%), Positives = 92/153 (60%), Gaps = 2/153 (1%) Query: 13 PKRTYWTRKKREQWAGWIFIAPEVXXXXXXXXXXXXXXXXXXXTEWNLVGGLSAIHFIGL 72 PKR + + E AGW+F++P + +WN V G + ++G Sbjct: 14 PKRVR-SLQSGETLAGWLFVSPMLIGVCILVLLPIVATIVLSFADWNFVQGWKGLDWVGF 72 Query: 73 DNFVNLFKDNHFILALKNNVLFTVGTVPLTMLLGIVLSALIHKKLYGKAFFKVAFFIPYI 132 NF LF D+ FI +++NN +F + VP+ ML+ +VL+ +I + +Y K++FKVA+F+PYI Sbjct: 73 GNFKKLFHDDQFIRSVRNNFIFLLA-VPIYMLISMVLAVIIDRHVYLKSYFKVAYFMPYI 131 Query: 133 CSTVAIAAVWQALYHPSKGPLNQMLMEMGISSP 165 S VA+A VWQ L+ PS GP+NQ+L G+S+P Sbjct: 132 SSIVAVAIVWQVLFQPSYGPINQILHTFGVSNP 164 >B3KA94.1 B3KA94_9BACI Binding-protein-dependent transport systems inner membrane component Length = 315 Score = 112 bits (279), Expect = 2e-23, Method: Compositional matrix adjust. Identities = 54/157 (34%), Positives = 92/157 (58%), Gaps = 1/157 (0%) Query: 9 EKTVPKRTYWTRKKREQWAGWIFIAPEVXXXXXXXXXXXXXXXXXXXTEWNLVGGLSAIH 68 + T ++ + ++ E G F++P + +WN V G I Sbjct: 14 QPTREDKSRGSLQRGENLWGIAFVSPMLIGVIILVLFPILATLVLGFADWNFVQGWDGIQ 73 Query: 69 FIGLDNFVNLFKDNHFILALKNNVLFTVGTVPLTMLLGIVLSALIHKKLYGKAFFKVAFF 128 ++G NF L +D+ FI +++NN+LF + TVP+ M++ + L+ LI + +Y K +FKVA+F Sbjct: 74 WVGFQNFRQLLEDDMFIKSVRNNILFLL-TVPVYMMISMTLAILIDRFVYMKGYFKVAYF 132 Query: 129 IPYICSTVAIAAVWQALYHPSKGPLNQMLMEMGISSP 165 +PYI + VA+A VWQ L+ PS GP+N++L +G+S+P Sbjct: 133 MPYISNIVAVAVVWQVLFQPSYGPINEILRTLGMSNP 169 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.326 0.140 0.447 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 946,666,413 Number of extensions: 30864517 Number of successful extensions: 128179 Number of sequences better than 1.0e-05: 2469 Number of HSP's gapped: 128283 Number of HSP's successfully gapped: 2469 Length of query: 179 Length of database: 3,060,696,203 Length adjustment: 127 Effective length of query: 52 Effective length of database: 1,864,227,298 Effective search space: 96939819496 Effective search space used: 96939819496 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 126 (53.1 bits)