BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0307.0086.p (132 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1D6F3.1 B1D6F3_9BACL Binding-protein-dependent transport system... 162 1e-38 B3K5Y6.1 B3K5Y6_9BACI Binding-protein-dependent transport system... 159 6e-38 A8VZX7.1 A8VZX7_9BACI Anti-sigma-factor antagonist and sulfate t... 154 3e-36 B1DAC3.1 B1DAC3_9BACL Binding-protein-dependent transport system... 144 4e-33 B3K442.1 B3K442_9BACI Binding-protein-dependent transport system... 142 1e-32 >B1D6F3.1 B1D6F3_9BACL Binding-protein-dependent transport systems inner membrane component Length = 310 Score = 162 bits (409), Expect = 1e-38, Method: Compositional matrix adjust. Identities = 77/118 (65%), Positives = 96/118 (81%) Query: 6 WQVLGYQMIIFLAGMTNIPEELYEAATIDGATGIQQFRRITVPLLGPTTFFLAITSTISS 65 W LGY +II+ AG+TNI E+YEA+ IDGA+ +++F RITVPLL PTTFFLAIT I S Sbjct: 188 WASLGYTIIIYTAGLTNISNEIYEASEIDGASPLKKFFRITVPLLRPTTFFLAITMLIGS 247 Query: 66 FKVFDMIKFLTNGGPNYSSTVIVYQIYEEGFERFKMGYASAMSWVLFLIIMLVTSITW 123 FKVFD+I +LT GGPN SSTV+VY+IYEEGF + MGYASA+SW+LF II ++T+ TW Sbjct: 248 FKVFDIISYLTEGGPNNSSTVLVYRIYEEGFRNYDMGYASAISWLLFAIIGIITAATW 305 >B3K5Y6.1 B3K5Y6_9BACI Binding-protein-dependent transport systems inner membrane component Length = 306 Score = 159 bits (403), Expect = 6e-38, Method: Compositional matrix adjust. Identities = 78/128 (60%), Positives = 101/128 (78%) Query: 2 IIYIWQVLGYQMIIFLAGMTNIPEELYEAATIDGATGIQQFRRITVPLLGPTTFFLAITS 61 II W LGY +II++AG+TNI E+YEAA IDGA +++F ITVP+L PTTFFL IT Sbjct: 179 IISSWAGLGYTIIIYMAGLTNISNEIYEAADIDGAGAMKKFFAITVPMLRPTTFFLLITM 238 Query: 62 TISSFKVFDMIKFLTNGGPNYSSTVIVYQIYEEGFERFKMGYASAMSWVLFLIIMLVTSI 121 I SFKVFD+I FLT GGPN SSTV+V++IYEEGF+ + MGYASA+SW+LF II L+T++ Sbjct: 239 LIGSFKVFDIIAFLTEGGPNNSSTVLVFRIYEEGFKYYNMGYASAISWLLFAIIGLITAL 298 Query: 122 TWITQSRK 129 TW ++ + Sbjct: 299 TWKMRNEE 306 >A8VZX7.1 A8VZX7_9BACI Anti-sigma-factor antagonist and sulfate transporter Length = 327 Score = 154 bits (388), Expect = 3e-36, Method: Compositional matrix adjust. Identities = 74/120 (61%), Positives = 94/120 (78%) Query: 10 GYQMIIFLAGMTNIPEELYEAATIDGATGIQQFRRITVPLLGPTTFFLAITSTISSFKVF 69 GY +II++AG++NIP+ LYEAA +DGA Q+F IT+PLLGPT FLAIT I SFKVF Sbjct: 204 GYVVIIYIAGLSNIPDSLYEAADVDGAKPYQKFIHITMPLLGPTHMFLAITLLIGSFKVF 263 Query: 70 DMIKFLTNGGPNYSSTVIVYQIYEEGFERFKMGYASAMSWVLFLIIMLVTSITWITQSRK 129 D+I FLT GGP SS V+VY IYEEGF+RF MGYASA+SW+LF ++ ++T +TW Q+ K Sbjct: 264 DLIAFLTQGGPINSSQVLVYLIYEEGFQRFNMGYASAISWLLFAVVGILTFLTWQVQNAK 323 >B1DAC3.1 B1DAC3_9BACL Binding-protein-dependent transport systems inner membrane component Length = 294 Score = 144 bits (362), Expect = 4e-33, Method: Compositional matrix adjust. Identities = 67/132 (50%), Positives = 96/132 (72%) Query: 1 MIIYIWQVLGYQMIIFLAGMTNIPEELYEAATIDGATGIQQFRRITVPLLGPTTFFLAIT 60 +I+ IW +GY M+++LAG+ IP++LYEAA IDGA + QFR IT+P+L P FF I Sbjct: 162 IIVSIWHSIGYYMVLYLAGLQGIPKDLYEAAEIDGAGKVSQFRNITLPMLSPVIFFTVIM 221 Query: 61 STISSFKVFDMIKFLTNGGPNYSSTVIVYQIYEEGFERFKMGYASAMSWVLFLIIMLVTS 120 I+SFKVFD++ LT GGP S+ ++VY IY+ F+R++ GYASAM++VLF II+++T Sbjct: 222 GIINSFKVFDLVYVLTKGGPGRSTHMLVYDIYDTAFKRYEYGYASAMAYVLFAIILVITL 281 Query: 121 ITWITQSRKVHY 132 I + Q R V+Y Sbjct: 282 IQFRGQKRWVNY 293 >B3K442.1 B3K442_9BACI Binding-protein-dependent transport systems inner membrane component Length = 318 Score = 142 bits (357), Expect = 1e-32, Method: Compositional matrix adjust. Identities = 67/130 (51%), Positives = 92/130 (70%) Query: 3 IYIWQVLGYQMIIFLAGMTNIPEELYEAATIDGATGIQQFRRITVPLLGPTTFFLAITST 62 + +W+ +G+ MI+F+AGM IP E YEAA+IDGA+ +QFR IT+PLL P T FL ITS Sbjct: 187 VSVWKGVGFAMILFIAGMRGIPAEYYEAASIDGASKFRQFRNITLPLLSPMTLFLVITSF 246 Query: 63 ISSFKVFDMIKFLTNGGPNYSSTVIVYQIYEEGFERFKMGYASAMSWVLFLIIMLVTSIT 122 ISS +VF I +T+GGP ++ IVY IY F+ FK G ASA+ +LF+II+L+T + Sbjct: 247 ISSMQVFQSIDVMTDGGPLKATNAIVYWIYTMAFDEFKTGRASALVMILFVIILLLTLVQ 306 Query: 123 WITQSRKVHY 132 W+ +KVHY Sbjct: 307 WVVSRKKVHY 316 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.328 0.141 0.424 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 771,325,352 Number of extensions: 27718002 Number of successful extensions: 121137 Number of sequences better than 1.0e-05: 5335 Number of HSP's gapped: 119755 Number of HSP's successfully gapped: 5335 Length of query: 132 Length of database: 3,060,696,203 Length adjustment: 97 Effective length of query: 35 Effective length of database: 2,146,857,748 Effective search space: 75140021180 Effective search space used: 75140021180 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 125 (52.8 bits)