BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0307.0085.p (284 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1D6F2.1 B1D6F2_9BACL Binding-protein-dependent transport system... 346 1e-93 A8VZX8.1 A8VZX8_9BACI Diguanylate cyclase 341 4e-92 B3K5Y5.1 B3K5Y5_9BACI Binding-protein-dependent transport system... 341 6e-92 A9KI81.1 A9KI81_CLOPH Binding-protein-dependent transport system... 287 1e-75 B3KA95.1 B3KA95_9BACI Binding-protein-dependent transport system... 284 7e-75 >B1D6F2.1 B1D6F2_9BACL Binding-protein-dependent transport systems inner membrane component Length = 275 Score = 346 bits (888), Expect = 1e-93, Method: Compositional matrix adjust. Identities = 160/272 (58%), Positives = 216/272 (79%) Query: 13 KTSNLILTILFALLSVFFLLPLIWMLSAAAKTERDVWTFPIQWIPKEWNLVSNFKEVWMG 72 K S I+T+L S+ FL PL+WM+S+A K E+DV FPIQWIP++ N + NFK VWMG Sbjct: 4 KLSKSIITVLLGAFSLMFLTPLVWMVSSAFKFEKDVMRFPIQWIPEKINFIYNFKSVWMG 63 Query: 73 DVSFGLFYMNSIKLAVISTLATVIISSMAGYALSKLEFKGRGIVFSLMLAFMMIPEQATL 132 V F FY NS+K+A+I+TL T+++SSM+ YAL+KL F+GR ++F ML+FM+IP+QATL Sbjct: 64 RVPFYHFYWNSLKVAIITTLITLLVSSMSAYALTKLRFRGRHMIFLAMLSFMIIPDQATL 123 Query: 133 VPRYIMIKDFGLYDSHAALILMGMFSSYFAFLLKQFMAGIHNDLLEAAELDGAGFFRIYR 192 +PR+++I+ GLYD+HAA+ILM MFS YF FLL+QFM+ I ++ LEAA +DGAG R + Sbjct: 124 IPRFLIIRWLGLYDTHAAIILMSMFSIYFTFLLRQFMSDISDEYLEAARIDGAGHLRTFF 183 Query: 193 SVMLPLCQPILVTVGIIKFIWTWNDYQGPLIMLNSTKLYTIPLGMQFFQEEFGTRISVMM 252 S+M+PLC+P+L TV IIKFIWTWNDYQ PLI L S KLYTIPLG+ F++++ ++MM Sbjct: 184 SIMIPLCRPVLATVAIIKFIWTWNDYQNPLIFLLSKKLYTIPLGITLFRDDYTNNYAIMM 243 Query: 253 MASVAAIIPLLVLFLALQKQVIEGIAIGGVKG 284 MA+++AIIPLL +F+ +QKQVI GIA+GGVKG Sbjct: 244 MAALSAIIPLLAIFIIMQKQVINGIALGGVKG 275 >A8VZX8.1 A8VZX8_9BACI Diguanylate cyclase Length = 275 Score = 341 bits (875), Expect = 4e-92, Method: Compositional matrix adjust. Identities = 166/278 (59%), Positives = 217/278 (78%), Gaps = 3/278 (1%) Query: 7 MSLKRIKTSNLILTILFALLSVFFLLPLIWMLSAAAKTERDVWTFPIQWIPKEWNLVSNF 66 M L ++K LI TI+ +LSVFFL PLIWMLSA+ K E DV FPI+WIP+ N+V+NF Sbjct: 1 MKLNKMK---LINTIVLGVLSVFFLAPLIWMLSASFKFESDVLVFPIEWIPRNINVVNNF 57 Query: 67 KEVWMGDVSFGLFYMNSIKLAVISTLATVIISSMAGYALSKLEFKGRGIVFSLMLAFMMI 126 K VWM ++ F LFY NSIKLA I TL T++ISSMA ++L+KL FKG+ I+F M+ M+I Sbjct: 58 KAVWMENIPFYLFYWNSIKLASIMTLGTLLISSMAAFSLTKLHFKGQNIIFFAMITLMII 117 Query: 127 PEQATLVPRYIMIKDFGLYDSHAALILMGMFSSYFAFLLKQFMAGIHNDLLEAAELDGAG 186 PEQ+TLVPR+IMI+ GLY++H ALI+MG+FS YF FL++Q+M I ND +EAA++DGA Sbjct: 118 PEQSTLVPRFIMIRWMGLYNTHEALIIMGIFSIYFTFLMRQYMLSIENDYIEAAKIDGAN 177 Query: 187 FFRIYRSVMLPLCQPILVTVGIIKFIWTWNDYQGPLIMLNSTKLYTIPLGMQFFQEEFGT 246 +F IY ++LPL +PIL TVGIIKFIW WNDYQ PLI L S +LY I +GMQ FQ++F Sbjct: 178 YFTIYWRIILPLARPILATVGIIKFIWVWNDYQNPLIFLFSKELYPITMGMQLFQDDFSN 237 Query: 247 RISVMMMASVAAIIPLLVLFLALQKQVIEGIAIGGVKG 284 +++MMASV+AIIPL+++F+ LQK VI GI++GGVKG Sbjct: 238 NYAILMMASVSAIIPLVIVFIILQKHVIAGISLGGVKG 275 >B3K5Y5.1 B3K5Y5_9BACI Binding-protein-dependent transport systems inner membrane component Length = 280 Score = 341 bits (874), Expect = 6e-92, Method: Compositional matrix adjust. Identities = 168/278 (60%), Positives = 219/278 (78%) Query: 7 MSLKRIKTSNLILTILFALLSVFFLLPLIWMLSAAAKTERDVWTFPIQWIPKEWNLVSNF 66 M L R S ++ T++ LS+ FL+PLIWM+SAA K E+DV FPIQWIP++ NL NF Sbjct: 3 MQLLRKNMSKVVTTVIMGALSIVFLVPLIWMISAAFKYEKDVMRFPIQWIPEKVNLAYNF 62 Query: 67 KEVWMGDVSFGLFYMNSIKLAVISTLATVIISSMAGYALSKLEFKGRGIVFSLMLAFMMI 126 K VWMG V F FY NS K+A+I+TL T+IISSMA YAL+K+ FKGR +VF +L+FM+I Sbjct: 63 KMVWMGRVPFYDFYFNSFKIAIITTLITLIISSMAAYALTKIRFKGRNLVFMALLSFMII 122 Query: 127 PEQATLVPRYIMIKDFGLYDSHAALILMGMFSSYFAFLLKQFMAGIHNDLLEAAELDGAG 186 P+QATL+PR+++I+ FGLY++HAA+I M MFS YF FLL+QFM GI ++ LEAA +DGAG Sbjct: 123 PDQATLIPRFLLIRWFGLYNTHAAIIFMSMFSIYFTFLLRQFMIGISDEYLEAARIDGAG 182 Query: 187 FFRIYRSVMLPLCQPILVTVGIIKFIWTWNDYQGPLIMLNSTKLYTIPLGMQFFQEEFGT 246 R + S+M+PLC+P+L TV IIKFIWTWNDYQ PLI L LYTIPLGM F++++ Sbjct: 183 HLRTFASIMVPLCKPVLATVAIIKFIWTWNDYQNPLIFLLDKDLYTIPLGMTLFRDDYTN 242 Query: 247 RISVMMMASVAAIIPLLVLFLALQKQVIEGIAIGGVKG 284 ++MMMA+V+AIIPL+++F+ALQKQVI GIA+GGVKG Sbjct: 243 NYAIMMMAAVSAIIPLVIVFIALQKQVINGIALGGVKG 280 >A9KI81.1 A9KI81_CLOPH Binding-protein-dependent transport systems inner membrane component Length = 281 Score = 287 bits (734), Expect = 1e-75, Method: Compositional matrix adjust. Identities = 131/274 (47%), Positives = 201/274 (73%) Query: 11 RIKTSNLILTILFALLSVFFLLPLIWMLSAAAKTERDVWTFPIQWIPKEWNLVSNFKEVW 70 R K ++ T++ ++++ +LPL+WM+SA+ K E DV+ +P++WIPK +N+++N+KEVW Sbjct: 8 RKKIIRIVATLVLGIMALTMVLPLLWMISASMKVEADVFKYPVEWIPKRFNVIANYKEVW 67 Query: 71 MGDVSFGLFYMNSIKLAVISTLATVIISSMAGYALSKLEFKGRGIVFSLMLAFMMIPEQA 130 G +FG+FY+NSIK+ +++T V+IS+M YA SK+EFK R +F L ++ MMIP+Q Sbjct: 68 SGRYNFGIFYLNSIKVTLLTTFLQVLISAMGAYAFSKIEFKFRDGLFLLYISMMMIPDQV 127 Query: 131 TLVPRYIMIKDFGLYDSHAALILMGMFSSYFAFLLKQFMAGIHNDLLEAAELDGAGFFRI 190 T++P++++ + L+D H LIL+G FS Y FLLKQFM I L E+A++DGAG RI Sbjct: 128 TIIPKFMIFRSLKLFDKHLGLILLGSFSIYGMFLLKQFMTAIPTALSESAKIDGAGHGRI 187 Query: 191 YRSVMLPLCQPILVTVGIIKFIWTWNDYQGPLIMLNSTKLYTIPLGMQFFQEEFGTRISV 250 + ++LP+ +P L T+ ++KF+WTWNDYQ PLI L + KLYTIPLGM F E+ + S+ Sbjct: 188 FLRIILPVTKPALATLAMLKFVWTWNDYQNPLIFLKTEKLYTIPLGMTKFMTEYTSFYSL 247 Query: 251 MMMASVAAIIPLLVLFLALQKQVIEGIAIGGVKG 284 +M+A+V AIIPLL++FL QK +++G+ +G VKG Sbjct: 248 IMVAAVCAIIPLLIVFLFGQKYIMDGMMVGAVKG 281 >B3KA95.1 B3KA95_9BACI Binding-protein-dependent transport systems inner membrane component Length = 279 Score = 284 bits (726), Expect = 7e-75, Method: Compositional matrix adjust. Identities = 132/269 (49%), Positives = 192/269 (71%) Query: 16 NLILTILFALLSVFFLLPLIWMLSAAAKTERDVWTFPIQWIPKEWNLVSNFKEVWMGDVS 75 LI+TI +S+ FLLP IWML + K ERDV+ +PIQWIP+EWN + N+KEVW GD Sbjct: 11 KLIVTIAMFAVSILFLLPFIWMLVTSFKVERDVFVYPIQWIPREWNAIQNYKEVWFGDYP 70 Query: 76 FGLFYMNSIKLAVISTLATVIISSMAGYALSKLEFKGRGIVFSLMLAFMMIPEQATLVPR 135 F L+Y NSIK+++++T+ + ++S++A Y SK+ F +F ++L MIP QA L+P+ Sbjct: 71 FYLYYWNSIKVSILTTIISAMVSALAAYGFSKIRFAAGNWLFIIVLVTYMIPGQAILIPQ 130 Query: 136 YIMIKDFGLYDSHAALILMGMFSSYFAFLLKQFMAGIHNDLLEAAELDGAGFFRIYRSVM 195 +I+ ++ GL+D+H LI++G FS F+L+QF G+H + +E+A++DGA RI+ S+ Sbjct: 131 FILYRNIGLFDNHFGLIMLGSFSVLGTFMLRQFFMGVHQEFIESAKIDGANHPRIFWSIA 190 Query: 196 LPLCQPILVTVGIIKFIWTWNDYQGPLIMLNSTKLYTIPLGMQFFQEEFGTRISVMMMAS 255 LPL +P + T I++FIWTWNDYQ PLI L + +L+TIPL MQ F G S++M A+ Sbjct: 191 LPLVKPAVATYAILRFIWTWNDYQNPLIFLRTDRLFTIPLAMQKFTSLSGEFYSLIMAAA 250 Query: 256 VAAIIPLLVLFLALQKQVIEGIAIGGVKG 284 V+AI PLL++F+ QK VIEGIA+GGVKG Sbjct: 251 VSAIFPLLIVFIIGQKSVIEGIALGGVKG 279 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.330 0.142 0.430 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,539,798,331 Number of extensions: 58634694 Number of successful extensions: 237596 Number of sequences better than 1.0e-05: 6327 Number of HSP's gapped: 228375 Number of HSP's successfully gapped: 6335 Length of query: 284 Length of database: 3,060,696,203 Length adjustment: 134 Effective length of query: 150 Effective length of database: 1,798,280,193 Effective search space: 269742028950 Effective search space used: 269742028950 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.9 bits) S2: 130 (54.7 bits)