BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0307.0065.p (410 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KAS2.1 B3KAS2_9BACI Type II secretion system protein E 550 e-155 B1DJH3.1 B1DJH3_9BACL Type II secretion system protein E 517 e-145 A3DF42.1 A3DF42_CLOTH Type II secretion system protein E 490 e-136 B4BEL5.1 B4BEL5_CLOTM Type II secretion system protein E 490 e-136 A9KQB3.1 A9KQB3_CLOPH Type II secretion system protein E 451 e-125 >B3KAS2.1 B3KAS2_9BACI Type II secretion system protein E Length = 415 Score = 550 bits (1418), Expect = e-155, Method: Compositional matrix adjust. Identities = 296/406 (72%), Positives = 342/406 (84%), Gaps = 1/406 (0%) Query: 1 MNEEMFRLLRSDIRSGLDLTSAVGNRELTAYIEQTVLARDSLRHLTAQEKHTLVKKLFDS 60 M E+ F+ +R +RS LD++++V N +L A IE+ V R L LTA EK LV+++FDS Sbjct: 1 MMEDPFKSVRDQVRSELDMSASVNNSQLMARIEEMVWRRRELMELTAAEKRRLVRRVFDS 60 Query: 61 FRXXXXLQPLVDNPAITEIMINSHDEIFVEENGLIRRLPLAFESSSRLEDIIQSVVSGVN 120 FR LQPLVDNP ITEIMINSH +IF+E++G + +LPL FES SRLEDIIQS+V VN Sbjct: 61 FRGLDVLQPLVDNPRITEIMINSHQDIFIEQDGEVNKLPLQFESQSRLEDIIQSIVGTVN 120 Query: 121 RVVNDSSPIVDARLKDGSRVNIVLPPVALKGPAMTIRKFPETPMTMNELIRRETVSMEAA 180 RVVN+S+PIVDARLKDGSRVNIVLPP+ALKGP MTIRKFPE+PMTM++L+ R ++ EAA Sbjct: 121 RVVNESTPIVDARLKDGSRVNIVLPPIALKGPTMTIRKFPESPMTMDDLVGRGALTEEAA 180 Query: 181 ELLQMLVAAKYNIFISGGTGSGKTTFLNALSQFIPPQERVITIEDSAELQIVTVPNLVSL 240 E L++LV KYNIFI GGTGSGKTTFLNALSQ+IPP ERVITIEDSAEL+IVTVPNLVSL Sbjct: 181 EQLRILVKGKYNIFIGGGTGSGKTTFLNALSQYIPPDERVITIEDSAELKIVTVPNLVSL 240 Query: 241 ETRNANTEGRGEISIRDLIRSSLRMRPNRIVVGEVRGAECLDMLQAMNTGHDGSLSTGHS 300 ETRNANTEGRGEI++RDLIRSSLRMRPNRIVVGEVRG+E LDMLQAMNTGHDGSLSTGH+ Sbjct: 241 ETRNANTEGRGEIAMRDLIRSSLRMRPNRIVVGEVRGSEALDMLQAMNTGHDGSLSTGHA 300 Query: 301 NSARDMVSRLETMVLSAADLPVAVVRQQIGSAIDIFVHLSRLRDRSRRVTEICEVAGVKE 360 N+ +DM+SRLETMVLS ADLP++VVRQQI SAIDIFVHLSRLRDRSRRVTEI EV G+ + Sbjct: 301 NNTQDMISRLETMVLSGADLPISVVRQQISSAIDIFVHLSRLRDRSRRVTEISEVIGMSD 360 Query: 361 GEVVLNPLYEFRETGGTDGHVQGGLIPSGNPMLHTGKLRMAGI-HW 405 GEVVLNPLY FRETG + G V G L P GNP+ KL MAGI W Sbjct: 361 GEVVLNPLYRFRETGESGGRVIGRLEPCGNPLQSADKLHMAGITAW 406 >B1DJH3.1 B1DJH3_9BACL Type II secretion system protein E Length = 417 Score = 517 bits (1332), Expect = e-145, Method: Compositional matrix adjust. Identities = 263/400 (65%), Positives = 322/400 (80%) Query: 9 LRSDIRSGLDLTSAVGNRELTAYIEQTVLARDSLRHLTAQEKHTLVKKLFDSFRXXXXLQ 68 L++ I+ LD + +V + +LTA +EQTV A S LTA E +LV++LF SFR LQ Sbjct: 13 LKNRIKDRLDYSGSVTDEQLTAIVEQTVFAWSSSNPLTASELASLVRRLFHSFRGLDILQ 72 Query: 69 PLVDNPAITEIMINSHDEIFVEENGLIRRLPLAFESSSRLEDIIQSVVSGVNRVVNDSSP 128 PL+D+P +TE+MIN H EIF+E G + LP +FES RLED+IQS+V+ VNRVVN+SSP Sbjct: 73 PLMDDPTVTEVMINHHSEIFIERGGRLSLLPASFESRERLEDLIQSIVASVNRVVNESSP 132 Query: 129 IVDARLKDGSRVNIVLPPVALKGPAMTIRKFPETPMTMNELIRRETVSMEAAELLQMLVA 188 IVDARLKDGSRV+IVLPPVAL+GPAMTIRKFPE P+TM+EL+ + ++ EAA LQ L Sbjct: 133 IVDARLKDGSRVHIVLPPVALRGPAMTIRKFPEKPLTMDELVAKGALTGEAAVFLQQLTK 192 Query: 189 AKYNIFISGGTGSGKTTFLNALSQFIPPQERVITIEDSAELQIVTVPNLVSLETRNANTE 248 A+YNIFISGGTG+GKTTFLNALSQ+IP ER++TIEDSAELQI +VPNLVSLETRNANTE Sbjct: 193 AQYNIFISGGTGTGKTTFLNALSQYIPQDERLVTIEDSAELQIRSVPNLVSLETRNANTE 252 Query: 249 GRGEISIRDLIRSSLRMRPNRIVVGEVRGAECLDMLQAMNTGHDGSLSTGHSNSARDMVS 308 G+GEI+IRDLIR+SLRMRPNRI+VGEVRG E LDML AMNTGH GS+STGHSN RDM+S Sbjct: 253 GKGEIAIRDLIRASLRMRPNRIIVGEVRGGEALDMLAAMNTGHAGSMSTGHSNGPRDMMS 312 Query: 309 RLETMVLSAADLPVAVVRQQIGSAIDIFVHLSRLRDRSRRVTEICEVAGVKEGEVVLNPL 368 RLETMV+SAA++PV V+R+QI SA+DI +HLSR RDRSRRVTEICEVAG+++GE+ + PL Sbjct: 313 RLETMVMSAAEMPVQVIRKQIASALDIVIHLSRFRDRSRRVTEICEVAGLEDGEIAVRPL 372 Query: 369 YEFRETGGTDGHVQGGLIPSGNPMLHTGKLRMAGIHWKGE 408 ++F E G G V G L + + HT KLRMAG+ E Sbjct: 373 FQFEEKGEEGGKVFGTLKRTDYKLYHTDKLRMAGMKLAEE 412 >A3DF42.1 A3DF42_CLOTH Type II secretion system protein E Length = 412 Score = 490 bits (1261), Expect = e-136, Method: Compositional matrix adjust. Identities = 243/405 (60%), Positives = 301/405 (74%), Gaps = 1/405 (0%) Query: 3 EEMFRLLRSDIRSGLDLTSAVGNRELTAYIEQTVLARDSLRHLTAQEKHTLVKKLFDSFR 62 E++ +R I LDL + E+ I V R +L+ EK + +F+S R Sbjct: 7 EKLIAQIRKHISENLDLRKDFSDEEIKDIITNVVFERSRDYYLSVGEKKEIADAIFNSMR 66 Query: 63 XXXXLQPLVDNPAITEIMINSHDEIFVEENGLIRRLPLAFESSSRLEDIIQSVVSGVNRV 122 LQPL+D+ +ITEIMIN D IF+E +G + +L + FES +LED+IQ++VS VNR Sbjct: 67 RLDVLQPLIDDKSITEIMINGPDSIFIERDGRVSKLNVKFESRRKLEDVIQTIVSRVNRT 126 Query: 123 VNDSSPIVDARLKDGSRVNIVLPPVALKGPAMTIRKFPETPMTMNELIRRETVSMEAAEL 182 VN++SPIVDARL DGSRVN+VLPP+AL GP +TIRKFPE PMT+ +LI+ +++ E AE+ Sbjct: 127 VNEASPIVDARLPDGSRVNVVLPPIALNGPVVTIRKFPEKPMTIEQLIKYGSITEEVAEV 186 Query: 183 LQMLVAAKYNIFISGGTGSGKTTFLNALSQFIPPQERVITIEDSAELQIVTVPNLVSLET 242 L+ LV AKYNIFI GGTGSGKTTFLNALS FIP ER++TIEDSAELQI V N+V LET Sbjct: 187 LERLVKAKYNIFICGGTGSGKTTFLNALSNFIPKDERIVTIEDSAELQITGVENIVRLET 246 Query: 243 RNANTEGRGEISIRDLIRSSLRMRPNRIVVGEVRGAECLDMLQAMNTGHDGSLSTGHSNS 302 RNANTEG+GEI+IRDLIR+SLRMRP RI+VGEVRG E LDMLQAMNTGHDGSLSTGH+NS Sbjct: 247 RNANTEGKGEITIRDLIRTSLRMRPERIIVGEVRGKEALDMLQAMNTGHDGSLSTGHANS 306 Query: 303 ARDMVSRLETMVLSAADLPVAVVRQQIGSAIDIFVHLSRLRDRSRRVTEICEVAGVKEGE 362 +DM+SRLETMVLS A++P+ +RQQI SAIDI +HL RLRD+SRR EI EV K G+ Sbjct: 307 TKDMLSRLETMVLSGAEMPLEAIRQQIASAIDIIIHLGRLRDKSRRTLEITEVVEYKNGQ 366 Query: 363 VVLNPLYEFRETGGT-DGHVQGGLIPSGNPMLHTGKLRMAGIHWK 406 +VLNPLYEF E G T + V G L + N M++ K +MAGI K Sbjct: 367 IVLNPLYEFVEEGETPEKQVIGTLRRTKNEMVNKLKFKMAGISDK 411 >B4BEL5.1 B4BEL5_CLOTM Type II secretion system protein E Length = 412 Score = 490 bits (1261), Expect = e-136, Method: Compositional matrix adjust. Identities = 243/405 (60%), Positives = 301/405 (74%), Gaps = 1/405 (0%) Query: 3 EEMFRLLRSDIRSGLDLTSAVGNRELTAYIEQTVLARDSLRHLTAQEKHTLVKKLFDSFR 62 E++ +R I LDL + E+ I V R +L+ EK + +F+S R Sbjct: 7 EKLIAQIRKHISENLDLRKDFSDEEIKDIITNVVFERSRDYYLSVGEKKEIADAIFNSMR 66 Query: 63 XXXXLQPLVDNPAITEIMINSHDEIFVEENGLIRRLPLAFESSSRLEDIIQSVVSGVNRV 122 LQPL+D+ +ITEIMIN D IF+E +G + +L + FES +LED+IQ++VS VNR Sbjct: 67 RLDVLQPLIDDKSITEIMINGPDSIFIERDGRVSKLNVKFESRRKLEDVIQTIVSRVNRT 126 Query: 123 VNDSSPIVDARLKDGSRVNIVLPPVALKGPAMTIRKFPETPMTMNELIRRETVSMEAAEL 182 VN++SPIVDARL DGSRVN+VLPP+AL GP +TIRKFPE PMT+ +LI+ +++ E AE+ Sbjct: 127 VNEASPIVDARLPDGSRVNVVLPPIALNGPVVTIRKFPEKPMTIEQLIKYGSITEEVAEV 186 Query: 183 LQMLVAAKYNIFISGGTGSGKTTFLNALSQFIPPQERVITIEDSAELQIVTVPNLVSLET 242 L+ LV AKYNIFI GGTGSGKTTFLNALS FIP ER++TIEDSAELQI V N+V LET Sbjct: 187 LERLVKAKYNIFICGGTGSGKTTFLNALSNFIPKDERIVTIEDSAELQITGVENIVRLET 246 Query: 243 RNANTEGRGEISIRDLIRSSLRMRPNRIVVGEVRGAECLDMLQAMNTGHDGSLSTGHSNS 302 RNANTEG+GEI+IRDLIR+SLRMRP RI+VGEVRG E LDMLQAMNTGHDGSLSTGH+NS Sbjct: 247 RNANTEGKGEITIRDLIRTSLRMRPERIIVGEVRGKEALDMLQAMNTGHDGSLSTGHANS 306 Query: 303 ARDMVSRLETMVLSAADLPVAVVRQQIGSAIDIFVHLSRLRDRSRRVTEICEVAGVKEGE 362 +DM+SRLETMVLS A++P+ +RQQI SAIDI +HL RLRD+SRR EI EV K G+ Sbjct: 307 TKDMLSRLETMVLSGAEMPLEAIRQQIASAIDIIIHLGRLRDKSRRTLEITEVVEYKNGQ 366 Query: 363 VVLNPLYEFRETGGT-DGHVQGGLIPSGNPMLHTGKLRMAGIHWK 406 +VLNPLYEF E G T + V G L + N M++ K +MAGI K Sbjct: 367 IVLNPLYEFVEEGETPEKQVIGTLRRTKNEMVNKLKFKMAGISDK 411 >A9KQB3.1 A9KQB3_CLOPH Type II secretion system protein E Length = 404 Score = 451 bits (1160), Expect = e-125, Method: Compositional matrix adjust. Identities = 225/396 (56%), Positives = 296/396 (74%), Gaps = 2/396 (0%) Query: 9 LRSDIRSGLDLTSAVGNRELTAYIEQTVLARDSLRHLTAQEKHTLVKKLFDSFRXXXXLQ 68 L+ I +G+D+T + EL +I++ + R +++ EK L ++F+S R LQ Sbjct: 8 LQEQILAGIDMTRELTEEELFDHIDEAIKVRGKEEYISITEKQRLRIEIFNSIRRLDVLQ 67 Query: 69 PLVDNPAITEIMINSHDEIFVEENGLIRRLPLAFESSSRLEDIIQSVVSGVNRVVNDSSP 128 LV++ +ITEIMIN EIFVE +G + + FES +LED+IQ++V+G NR++N++SP Sbjct: 68 ELVEDTSITEIMINGAKEIFVERDGKLMKWEKEFESDRKLEDVIQTIVAGANRIINEASP 127 Query: 129 IVDARLKDGSRVNIVLPPVALKGPAMTIRKFPETPMTMNELIRRETVSMEAAELLQMLVA 188 IVDARLKDGSRVNIVLPP+A+ GP +TIRKFP+ MTM +L+ +++ EAAE L+ LV Sbjct: 128 IVDARLKDGSRVNIVLPPIAIGGPTVTIRKFPKETMTMEKLVTIGSLTEEAAEFLKKLVI 187 Query: 189 AKYNIFISGGTGSGKTTFLNALSQFIPPQERVITIEDSAELQIVTVPNLVSLETRNANTE 248 A YNIF+SGGTGSGKTTFLNALS ++P +ER+ITIEDSAELQI +PNLV LE RNAN E Sbjct: 188 AGYNIFVSGGTGSGKTTFLNALSNYVPSEERIITIEDSAELQIRNIPNLVRLEVRNANVE 247 Query: 249 GRGEISIRDLIRSSLRMRPNRIVVGEVRGAECLDMLQAMNTGHDGSLSTGHSNSARDMVS 308 G+ EISIRDLI+SSLRMRP+RI+VGEVR A +DMLQA+NTGH+G +STGHSNS DM+S Sbjct: 248 GKNEISIRDLIKSSLRMRPDRIIVGEVRDASSIDMLQALNTGHNG-MSTGHSNSPTDMLS 306 Query: 309 RLETMVLSAADLPVAVVRQQIGSAIDIFVHLSRLRDRSRRVTEICEVAGVKEGEVVLNPL 368 RLETMVL AD+P+ VR+QI SAIDI +HL RLRD++R+V E+ EV +GE+ +NPL Sbjct: 307 RLETMVLLGADIPLLAVRKQIASAIDIVIHLGRLRDKTRKVLEVVEVLECVDGEIEVNPL 366 Query: 369 YEFRETGGT-DGHVQGGLIPSGNPMLHTGKLRMAGI 403 + F E G T +G V G L + N +LH KL AG+ Sbjct: 367 FLFSEEGETKEGKVLGELKKTDNSLLHVQKLLRAGL 402 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.318 0.135 0.372 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,239,709,808 Number of extensions: 88841831 Number of successful extensions: 369831 Number of sequences better than 1.0e-05: 3072 Number of HSP's gapped: 367586 Number of HSP's successfully gapped: 3073 Length of query: 410 Length of database: 3,060,696,203 Length adjustment: 138 Effective length of query: 272 Effective length of database: 1,760,596,133 Effective search space: 478882148176 Effective search space used: 478882148176 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)