BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0307.0064.p (267 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KAS3.1 B3KAS3_9BACI Type II secretion system protein 258 5e-67 A3DF41.1 A3DF41_CLOTH Flp pilus assembly protein TadB-like protein 189 3e-46 B4BEL4.1 B4BEL4_CLOTM Flp pilus assembly protein TadB-like protein 189 3e-46 B1DJH4.1 B1DJH4_9BACL Type II secretion system protein 178 4e-43 B8I6J4.1 B8I6J4_CLOCE Flp pilus assembly protein TadB-like protein 139 3e-31 >B3KAS3.1 B3KAS3_9BACI Type II secretion system protein Length = 285 Score = 258 bits (658), Expect = 5e-67, Method: Compositional matrix adjust. Identities = 134/261 (51%), Positives = 174/261 (66%), Gaps = 3/261 (1%) Query: 10 VAPKGNPGAAAQ---LPDYTVYVLASWHKITAMLVGGTLLWAIGYLFYHNXXXXXXXXXX 66 +A K AAA+ LPDY+VY L+ H+ +++GG L IGYLFYHN Sbjct: 25 IAGKPEHAAAARTHALPDYSVYHLSMRHRTACVILGGLLFCFIGYLFYHNLILSLLLGAG 84 Query: 67 XXXXPXXXXXXXXXXXXXXXNLQFKQMLFXXXXXXXAGRSVENAFREAVQDLLMLDPEGD 126 P NL FKQ L+ AGRSVEN FR+AVQDLL+LDP G Sbjct: 85 GLMIPKLWRRFMLERRRRTLNLHFKQALYSLSSSLSAGRSVENGFRDAVQDLLLLDPGGS 144 Query: 127 SDMISELNIICARMEYGQPVEEALNDFSKRAGMEDVERFADVFSVCKRTGGDLVEIVRRT 186 SD+I EL +I +R+EYG+P+E AL DF++RAGMED+ FADVFS CKRTGGDLVE+VRRT Sbjct: 145 SDLIFELKVIVSRLEYGEPIEVALQDFARRAGMEDLTNFADVFSTCKRTGGDLVEVVRRT 204 Query: 187 STIIGEKLDMQQEIAVSIAQKKFEAKALLVSPLIMVTFMSLTAGDYMQPMYTGAGIAIST 246 S++IGEKLD+QQEI+V IAQK+FE+KALL +P +++ FM++T+ DYM+PMY+G+G IST Sbjct: 205 SSVIGEKLDIQQEISVMIAQKRFESKALLAAPFLLLLFMNMTSPDYMEPMYSGSGFMIST 264 Query: 247 XXXXXXXXXXXWTSKIMNIPL 267 W +K+MNI + Sbjct: 265 FALAALGGCFLWITKMMNIKV 285 >A3DF41.1 A3DF41_CLOTH Flp pilus assembly protein TadB-like protein Length = 268 Score = 189 bits (479), Expect = 3e-46, Method: Compositional matrix adjust. Identities = 97/259 (37%), Positives = 147/259 (56%), Gaps = 4/259 (1%) Query: 10 VAPKGNPGAAAQLPDYTVYVLASWHKITAMLVGGTLLWAIGYLFYHNXXXXXXXXXXXXX 69 VA K N L DY VYV+++ K+ +L+ ++L+AIGY+FYH+ Sbjct: 13 VAAKQNYSG---LIDYDVYVMSAKEKVFYVLLAASVLFAIGYIFYHHIIGALILTPLALL 69 Query: 70 XPXXXXXXXXXXXXXXXNLQFKQMLFXXXXXXXAGRSVENAFREAVQDLLMLDPEGDSDM 129 P NLQFK +L+ AG+SVE AF+EA+ DL +L P+ D+ + Sbjct: 70 YPKRKTKDIIEKRKSELNLQFKDLLYSISASLTAGKSVETAFKEALNDLCILYPDPDTYI 129 Query: 130 ISELNIICARMEYGQPVEEALNDFSKRAGMEDVERFADVFSVCKRTGGDLVEIVRRTSTI 189 I E+ I R+E + +E+AL D +KR+ +ED++ F DVF CKRTGG+LV ++R ++ I Sbjct: 130 IREVEYIVRRLEMNETIEDALEDLAKRSHLEDIQNFTDVFKTCKRTGGNLVNVIRNSTNI 189 Query: 190 IGEKLDMQQEIAVSIAQKKFEAKALLVSPLIMVTFMSLTAGDYMQPMY-TGAGIAISTXX 248 I +K+++++EI +A KKFE K L V P IM+ +SLT DYM P++ T G + T Sbjct: 190 INDKIEIKEEINTLLAAKKFEQKVLSVMPFIMILILSLTTEDYMAPVFDTIVGRIVMTFA 249 Query: 249 XXXXXXXXXWTSKIMNIPL 267 + KIM+I + Sbjct: 250 IIIIAIGYFVSKKIMDIKI 268 >B4BEL4.1 B4BEL4_CLOTM Flp pilus assembly protein TadB-like protein Length = 268 Score = 189 bits (479), Expect = 3e-46, Method: Compositional matrix adjust. Identities = 97/259 (37%), Positives = 147/259 (56%), Gaps = 4/259 (1%) Query: 10 VAPKGNPGAAAQLPDYTVYVLASWHKITAMLVGGTLLWAIGYLFYHNXXXXXXXXXXXXX 69 VA K N L DY VYV+++ K+ +L+ ++L+AIGY+FYH+ Sbjct: 13 VAAKQNYSG---LIDYDVYVMSAKEKVFYVLLAASVLFAIGYIFYHHIIGALILTPLALL 69 Query: 70 XPXXXXXXXXXXXXXXXNLQFKQMLFXXXXXXXAGRSVENAFREAVQDLLMLDPEGDSDM 129 P NLQFK +L+ AG+SVE AF+EA+ DL +L P+ D+ + Sbjct: 70 YPKRKTKDIIEKRKSELNLQFKDLLYSISASLTAGKSVETAFKEALNDLCILYPDPDTYI 129 Query: 130 ISELNIICARMEYGQPVEEALNDFSKRAGMEDVERFADVFSVCKRTGGDLVEIVRRTSTI 189 I E+ I R+E + +E+AL D +KR+ +ED++ F DVF CKRTGG+LV ++R ++ I Sbjct: 130 IREVEYIVRRLEMNETIEDALEDLAKRSHLEDIQNFTDVFKTCKRTGGNLVNVIRNSTNI 189 Query: 190 IGEKLDMQQEIAVSIAQKKFEAKALLVSPLIMVTFMSLTAGDYMQPMY-TGAGIAISTXX 248 I +K+++++EI +A KKFE K L V P IM+ +SLT DYM P++ T G + T Sbjct: 190 INDKIEIKEEINTLLAAKKFEQKVLSVMPFIMILILSLTTEDYMAPVFDTIVGRIVMTFA 249 Query: 249 XXXXXXXXXWTSKIMNIPL 267 + KIM+I + Sbjct: 250 IIIIAIGYFVSKKIMDIKI 268 >B1DJH4.1 B1DJH4_9BACL Type II secretion system protein Length = 287 Score = 178 bits (452), Expect = 4e-43, Method: Compositional matrix adjust. Identities = 89/244 (36%), Positives = 139/244 (56%) Query: 22 LPDYTVYVLASWHKITAMLVGGTLLWAIGYLFYHNXXXXXXXXXXXXXXPXXXXXXXXXX 81 L DY Y+L+ + + VG +++A YL YH+ P Sbjct: 42 LQDYNQYLLSYKQFMGSAAVGFVIIFAAVYLIYHSFIVSGLFAAGGLAAPRMYKSFLLVR 101 Query: 82 XXXXXNLQFKQMLFXXXXXXXAGRSVENAFREAVQDLLMLDPEGDSDMISELNIICARME 141 LQFK+ L+ AGRSVENAF + DL +L P+ +++++ E I+ R++ Sbjct: 102 RKERLQLQFKEALYSLASSLAAGRSVENAFLATIDDLKLLYPDPNTELLLEFRIVKYRLD 161 Query: 142 YGQPVEEALNDFSKRAGMEDVERFADVFSVCKRTGGDLVEIVRRTSTIIGEKLDMQQEIA 201 G+P+E+AL +KR ++D+ +F+DVF+VCKR+GGDLVE+++RTS IGEKL+++QEI+ Sbjct: 162 NGEPLEQALRSLAKRTHLDDITQFSDVFAVCKRSGGDLVEVMKRTSQTIGEKLEVKQEIS 221 Query: 202 VSIAQKKFEAKALLVSPLIMVTFMSLTAGDYMQPMYTGAGIAISTXXXXXXXXXXXWTSK 261 V +AQK+FEA+ ++ P + F+S A DYM+P+Y G G + T + Sbjct: 222 VLLAQKRFEARIMMAVPFAFLGFLSAFAPDYMKPLYGGGGYVLLTIGLVVLSFCFWLIHR 281 Query: 262 IMNI 265 IM I Sbjct: 282 IMQI 285 >B8I6J4.1 B8I6J4_CLOCE Flp pilus assembly protein TadB-like protein Length = 247 Score = 139 bits (349), Expect = 3e-31, Method: Compositional matrix adjust. Identities = 76/245 (31%), Positives = 128/245 (52%), Gaps = 1/245 (0%) Query: 24 DYTVYVLASWHKITAMLVGGTLLWAIGYLFYHNXXXXXXXXXXXXXXPXXXXXXXXXXXX 83 DY Y + + K+ L T+++A+ YLFY + Sbjct: 3 DYNEYAIPTKMKLVYALSAATVVFAVSYLFYRSFTVSIAISPLGIFYLNYRRKQIIEKRK 62 Query: 84 XXXNLQFKQMLFXXXXXXXAGRSVENAFREAVQDLLMLDPEGDSDMISELNIICARMEYG 143 NLQFK +L AG+ +E AF A+ DL +L P D+ ++ E II ++ Sbjct: 63 NELNLQFKDLLISLSSSLSAGKPLEKAFVGALSDLYVLYPSDDAYIVRETKIIINKLSLN 122 Query: 144 QPVEEALNDFSKRAGMEDVERFADVFSVCKRTGGDLVEIVRRTSTIIGEKLDMQQEIAVS 203 +EEA++DF++R+ ++D++ F+DV +CKRTGG+LV+ ++ +S II EK++M QEI Sbjct: 123 VSIEEAVSDFAERSDIDDIKNFSDVIIICKRTGGNLVDAIKNSSRIISEKIEMAQEIETL 182 Query: 204 IAQKKFEAKALLVSPLIMVTFMSLTAGDYMQPMYTGAGIAISTXXXXXXXXXXXWTSK-I 262 ++ +K E K L + P+ M+ +S TA +Y+ P++T I+ + SK I Sbjct: 183 LSSRKLEQKVLNIMPIAMIFILSTTATEYINPVFTTVTGRIAATLCLILLTAAYFISKRI 242 Query: 263 MNIPL 267 +NI + Sbjct: 243 INIKM 247 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.321 0.135 0.396 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,235,674,021 Number of extensions: 42865530 Number of successful extensions: 110371 Number of sequences better than 1.0e-05: 59 Number of HSP's gapped: 110353 Number of HSP's successfully gapped: 59 Length of query: 267 Length of database: 3,060,696,203 Length adjustment: 133 Effective length of query: 134 Effective length of database: 1,807,701,208 Effective search space: 242231961872 Effective search space used: 242231961872 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 130 (54.7 bits)