BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0307.0063.p (290 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KAS4.1 B3KAS4_9BACI Type II secretion system protein 324 6e-87 A9U6N7.1 A9U6N7_PHYPA Predicted protein 249 3e-64 B1DJH5.1 B1DJH5_9BACL Type II secretion system protein 149 2e-34 A5ZWB6.1 A5ZWB6_9FIRM Putative uncharacterized protein 103 2e-20 A3DF40.1 A3DF40_CLOTH Type II secretion system protein 99 6e-19 >B3KAS4.1 B3KAS4_9BACI Type II secretion system protein Length = 290 Score = 324 bits (830), Expect = 6e-87, Method: Compositional matrix adjust. Identities = 163/268 (60%), Positives = 193/268 (72%), Gaps = 1/268 (0%) Query: 23 CGSRYAVLRTLPMEGLRLRGLGEPFLWMIERWKPGRYFPSVMFRIQRSLQRTYGTRYSAE 82 C RY L L M+GLRL+ L P L+++ER K FP V +R+Q S+Q+ +G R+SAE Sbjct: 24 CSERYHRLGRLSMDGLRLQRLAPPVLFLLERGKVPMRFPIVFYRMQGSVQKIHGLRHSAE 83 Query: 83 RTLLFMGEMVSYSWLFLTAGSVLTVLSGETAGTVLGTFLAVMLPAALVSDLHKKVRLREQ 142 TLLFM E ++Y+WL L G + ++LSGE G + G L +LP ALV DLHKKV LREQ Sbjct: 84 MTLLFMAEALAYTWLILIGGCLWSLLSGEVIGLLAGFGLGALLPVALVKDLHKKVTLREQ 143 Query: 143 NISFELPELLNSIVLLVGAGETVQRAIIRCVDSRKGDTSHPLYKELHQMTAEWDSGYSFQ 202 +I ELPELLN IVLLVGAGETVQRAII CV+ R D SHPLY EL +M EW+ G+ FQ Sbjct: 144 DIVMELPELLNKIVLLVGAGETVQRAIIHCVE-RNEDASHPLYAELKRMVLEWEGGHPFQ 202 Query: 203 QAFENFSKRCAVQEVSLFTTTVLLNYRRGGADFVLALRDLSRMLWEKRKAISRTRGEQAS 262 QAFE F+KRC VQEVS+FTTTVLLN+RRGG DFVLALRDLSR+LWEKRKAI+RTRGEQAS Sbjct: 203 QAFEQFNKRCGVQEVSIFTTTVLLNFRRGGNDFVLALRDLSRVLWEKRKAIARTRGEQAS 262 Query: 263 SKXXXXXXXXXXXXXXXXGTPAFMMLKM 290 SK G PAFMM+ M Sbjct: 263 SKLVLPMVVIFMIVIVLLGAPAFMMMNM 290 >A9U6N7.1 A9U6N7_PHYPA Predicted protein Length = 200 Score = 249 bits (635), Expect = 3e-64, Method: Compositional matrix adjust. Identities = 121/200 (60%), Positives = 143/200 (71%) Query: 91 MVSYSWLFLTAGSVLTVLSGETAGTVLGTFLAVMLPAALVSDLHKKVRLREQNISFELPE 150 M+ YSWL L G +LT+ +G G ++G LA +LPAA + DLH KV R+Q+I+ EL E Sbjct: 1 MLCYSWLMLMLGCLLTLAAGGNTGLIIGGLLAFLLPAATIKDLHTKVDKRDQDITIELAE 60 Query: 151 LLNSIVLLVGAGETVQRAIIRCVDSRKGDTSHPLYKELHQMTAEWDSGYSFQQAFENFSK 210 LLN IVLLVGAGETVQ+AI RC ++ HPLYKEL +M + + GYSFQQ+FENFSK Sbjct: 61 LLNKIVLLVGAGETVQKAITRCARQKEAQRDHPLYKELIRMIDDLEGGYSFQQSFENFSK 120 Query: 211 RCAVQEVSLFTTTVLLNYRRGGADFVLALRDLSRMLWEKRKAISRTRGEQASSKXXXXXX 270 RCAVQEVS FTT VLLN RRGG+DF +AL DLSR LWEKRKAI+RTRGEQASSK Sbjct: 121 RCAVQEVSAFTTAVLLNLRRGGSDFAMALNDLSRGLWEKRKAITRTRGEQASSKLVFPMM 180 Query: 271 XXXXXXXXXXGTPAFMMLKM 290 GTPAFMM+ M Sbjct: 181 VIFMIVIVLVGTPAFMMMNM 200 >B1DJH5.1 B1DJH5_9BACL Type II secretion system protein Length = 282 Score = 149 bits (377), Expect = 2e-34, Method: Compositional matrix adjust. Identities = 87/213 (40%), Positives = 123/213 (57%), Gaps = 1/213 (0%) Query: 76 GTRYSAERTLLFMGEMVSYSWLFLTAGSVLTVLSGETAGTVLGTFLAVMLPAALVSDLHK 135 GT + +T + E + + L ++L VL+ E +G L ++LP + + Sbjct: 70 GTDWQLNQTKQHIAETTGFGYAVLCVCTLLAVLAKEPLLIGMGVLLGLLLPVRPFMEAGR 129 Query: 136 KVRLREQNISFELPELLNSIVLLVGAGETVQRAIIRCVDSRKGDTSHPLYKELHQMTAEW 195 V R+Q I ELPE L+ ++LLVGAGE V +A RC++ K PLYKE ++ Sbjct: 130 LVERRKQAIVSELPEALSKLMLLVGAGEPVLQAFSRCLEG-KDPAVQPLYKEWDRVVIAL 188 Query: 196 DSGYSFQQAFENFSKRCAVQEVSLFTTTVLLNYRRGGADFVLALRDLSRMLWEKRKAISR 255 +G SF A E F++ CAVQEVS+FTT +LLNYRRGG FVLALR++S LWEKRKA++R Sbjct: 189 RNGESFSTALERFNRSCAVQEVSIFTTVILLNYRRGGDQFVLALREISYSLWEKRKAVAR 248 Query: 256 TRGEQASSKXXXXXXXXXXXXXXXXGTPAFMML 288 +RGE+ASSK PA +++ Sbjct: 249 SRGEEASSKLVFPLVGILLVLMVFVAAPAMLLM 281 >A5ZWB6.1 A5ZWB6_9FIRM Putative uncharacterized protein Length = 289 Score = 103 bits (257), Expect = 2e-20, Method: Compositional matrix adjust. Identities = 56/196 (28%), Positives = 104/196 (53%), Gaps = 4/196 (2%) Query: 69 RSLQRTYGTRYSAERTLLFMGEMVSYSWLFLTAGSVLTVLSGETAGTVLGTFLAVMLPAA 128 + + G +Y+ + G ++Y++ +L+VL+G +LG +V++ Sbjct: 73 KEIAEIKGKKYAEYYYYILQGAKITYAYTVFLVFMLLSVLAGSVEAALLGVIFSVLVVMY 132 Query: 129 LVSDLHKKVRLREQNISFELPELLNSIVLLVGAGETVQRAIIRCVDSRKGDTSHPLYKEL 188 + L K+ R Q I +LP++L+ + LLV +G ++ A R + + LYKE+ Sbjct: 133 IDLSLQDKLTARRQEILMDLPQVLSKLTLLVNSGMVLRDAWKRTAITGE----RALYKEM 188 Query: 189 HQMTAEWDSGYSFQQAFENFSKRCAVQEVSLFTTTVLLNYRRGGADFVLALRDLSRMLWE 248 + E D+G A+ NF+ RC+V+EV FT+ +L N ++G + L L D+S +WE Sbjct: 189 QNTSLEIDNGIMEADAYRNFADRCSVKEVRKFTSLILQNLQKGNEELALFLGDMSTEMWE 248 Query: 249 KRKAISRTRGEQASSK 264 +K + +GE+A+S+ Sbjct: 249 MKKNEVKQKGEKANSR 264 >A3DF40.1 A3DF40_CLOTH Type II secretion system protein Length = 289 Score = 98.6 bits (244), Expect = 6e-19, Method: Compositional matrix adjust. Identities = 59/181 (32%), Positives = 91/181 (50%), Gaps = 2/181 (1%) Query: 108 LSGETAGTVLGTFLAVMLPAALVSDLHKKVRLREQNISFELPELLNSIVLLVGAGETVQR 167 ++ + A V G + +P + +L KV R +I + P+ LN ++LLV AG TV R Sbjct: 107 VTQDVATLVFGIAVTAAVPYFMDRELSGKVNRRRLSIQLDFPDFLNKVILLVNAGMTVSR 166 Query: 168 AIIRCVDSRKGDTSHPLYKELHQMTAEWDSGYSFQQAFENFSKRCAVQEVSLFTTTVLLN 227 A + V + DT LY+EL + A+ G S AFE+F+ RC + E++ F + +L N Sbjct: 167 AWEKSVTDSEKDTV--LYQELRTVLADIRGGKSETSAFEDFANRCRIPEITKFVSALLQN 224 Query: 228 YRRGGADFVLALRDLSRMLWEKRKAISRTRGEQASSKXXXXXXXXXXXXXXXXGTPAFMM 287 +G A+ V LR + WE RK ++ GE+AS+K TPA + Sbjct: 225 LNKGNAEMVSILRLQAAECWEMRKHAAKRLGEEASTKMLFPMMLMFIAVILIVATPAVLT 284 Query: 288 L 288 L Sbjct: 285 L 285 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.324 0.136 0.404 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,408,012,244 Number of extensions: 52633931 Number of successful extensions: 168631 Number of sequences better than 1.0e-05: 31 Number of HSP's gapped: 168732 Number of HSP's successfully gapped: 31 Length of query: 290 Length of database: 3,060,696,203 Length adjustment: 134 Effective length of query: 156 Effective length of database: 1,798,280,193 Effective search space: 280531710108 Effective search space used: 280531710108 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 130 (54.7 bits)