BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0307.0058.p
         (169 letters)

Database: pfamseq 
           9,421,015 sequences; 3,060,696,203 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

B3KAS9.1 B3KAS9_9BACI Peptidase A24A prepilin type IV                  98   2e-19
B1DJI0.1 B1DJI0_9BACL Peptidase A24A prepilin type IV                  63   7e-09
C1ZBL0.1 C1ZBL0_PLALI Flp pilus assembly protein, protease CpaA        57   7e-07
C0GEA6.1 C0GEA6_9FIRM Peptidase A24A prepilin type IV                  53   8e-06

>B3KAS9.1 B3KAS9_9BACI Peptidase A24A prepilin type IV
          Length = 170

 Score = 98.2 bits (243), Expect = 2e-19,   Method: Compositional matrix adjust.
 Identities = 64/169 (37%), Positives = 87/169 (51%), Gaps = 1/169 (0%)

Query: 1   MAVWAFWSCLPFLAAAFLTDIRFMRIPNWITLPLLMAGLIVQGMMNGRQGFLVSICGAXX 60
           MAV AF  C  +L AAF+TD+R M+IPN +T+  +++G +  G ++G +G L S+ G   
Sbjct: 1   MAV-AFGICAVYLIAAFITDLRSMKIPNALTVSAMLSGPVYHGALDGWEGLLFSLKGLGA 59

Query: 61  XXXXXXXXXXXXXXXAGDVKLFTGIGAWTGILFTLQVTVYSVLFGAVIGWIIVLXXXXXX 120
                          AGDVKLF GIGAWTG+ FTLQ  +YSVLF   IG +I+L      
Sbjct: 60  GFLILLIMYFIGAVGAGDVKLFGGIGAWTGLWFTLQGIMYSVLFAGAIGLLILLWRRETM 119

Query: 121 XXXXXXXXXXXXFLMLREPXXXXXXXXXXXXFPFMLAVVPGFICAYLYL 169
                         +++              FPFM+AV+PG I  YL L
Sbjct: 120 KRVRHVVESIAGVFVMKSWLPWRNSQEEHLRFPFMMAVLPGMISTYLVL 168


>B1DJI0.1 B1DJI0_9BACL Peptidase A24A prepilin type IV
          Length = 171

 Score = 63.2 bits (152), Expect = 7e-09,   Method: Compositional matrix adjust.
 Identities = 45/148 (30%), Positives = 65/148 (43%), Gaps = 8/148 (5%)

Query: 19  TDIRFMRIPNWITLPLLMAGLIVQGMMNGRQGFLVSICGAXXXXXXXXXXXXXXXXXAGD 78
           TD++ M IPN +T+P L  G +      G  G L++I GA                 AGD
Sbjct: 18  TDLKSMTIPNLLTVPFLAGGFVYALFSGGGHGLLLAIYGAAAGFVPLLILHLAKGIGAGD 77

Query: 79  VKLFTGIGAWTGILFTLQVTVYSVLFGAVIGWIIVLXXXXXXXXXXXXXXXXXXFLMLRE 138
           VKLF  +GAW G L  LQ+ +Y++L+  ++G ++++                  F+ LR 
Sbjct: 78  VKLFAALGAWIGTLAVLQLMMYAILYAGLVGLVLLV---FHRPFARKMTAGMYTFVSLRF 134

Query: 139 PXXXXXX-----XXXXXXFPFMLAVVPG 161
                             FPFMLAV PG
Sbjct: 135 GGQAGASSWLAWAKNGQTFPFMLAVAPG 162


>C1ZBL0.1 C1ZBL0_PLALI Flp pilus assembly protein, protease CpaA
          Length = 183

 Score = 56.6 bits (135), Expect = 7e-07,   Method: Compositional matrix adjust.
 Identities = 33/89 (37%), Positives = 47/89 (52%)

Query: 20  DIRFMRIPNWITLPLLMAGLIVQGMMNGRQGFLVSICGAXXXXXXXXXXXXXXXXXAGDV 79
           D + +R+PNWIT P++++GLI   +M G  G   S+ G                  AGDV
Sbjct: 27  DGKELRVPNWITFPMVLSGLIYGFVMGGWAGLGDSLIGMVVGLACLLPLYAVGGMGAGDV 86

Query: 80  KLFTGIGAWTGILFTLQVTVYSVLFGAVI 108
           KL  G+GAW G   T Q  + SV+ GA++
Sbjct: 87  KLMAGMGAWLGAAITWQAFMVSVVVGAIM 115


>C0GEA6.1 C0GEA6_9FIRM Peptidase A24A prepilin type IV
          Length = 193

 Score = 53.1 bits (126), Expect = 8e-06,   Method: Compositional matrix adjust.
 Identities = 32/103 (31%), Positives = 50/103 (48%), Gaps = 2/103 (1%)

Query: 12  FLAAAFLTDIRFMRIPNWITLPLLMAGLIVQGMMNGRQGFLVSICGAXXXXXXXXXXXXX 71
           F+A A   D+R  RIPN IT P+++ GL++ G + G  G      G              
Sbjct: 8   FVALAAYFDVRQRRIPNKITFPIMIVGLVIYGFLEGLPGIAFGFTGLLSGLFVFFIPFAL 67

Query: 72  XXXXAGDVKLFTGIGAWTGILFTLQVTVYSVLFGAV--IGWII 112
               AGDVK+   +GA  G+ F L   ++++  G +  IG++I
Sbjct: 68  GGMGAGDVKMTAAVGALMGMQFVLHSAIFTIFAGGIMAIGYLI 110


  Database: pfamseq
    Posted date:  Aug 5, 2010  3:22 PM
  Number of letters in database: 3,060,696,203
  Number of sequences in database:  9,421,015
  
Lambda     K      H
   0.337    0.149    0.512 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 9421015
Number of Hits to DB: 691,937,962
Number of extensions: 17172440
Number of successful extensions: 83214
Number of sequences better than 1.0e-05: 13
Number of HSP's gapped: 83289
Number of HSP's successfully gapped: 13
Length of query: 169
Length of database: 3,060,696,203
Length adjustment: 125
Effective length of query: 44
Effective length of database: 1,883,069,328
Effective search space: 82855050432
Effective search space used: 82855050432
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 39 (21.7 bits)
S2: 126 (53.1 bits)