BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0307.0054.p (368 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KAT4.1 B3KAT4_9BACI Apurinic endonuclease Apn1 EC=3.1.21.2 575 e-162 B1DJI5.1 B1DJI5_9BACL Apurinic endonuclease Apn1 EC=3.1.21.2 543 e-152 C0ZIS9.1 C0ZIS9_BREBN Endonuclease IV EC=3.1.21.2 516 e-144 A8VUC2.1 A8VUC2_9BACI Deoxyribonuclease IV (Phage-T(4)-induced) ... 343 1e-92 C5D4R0.1 C5D4R0_9BACI Apurinic endonuclease Apn1 EC=3.1.21.2 340 2e-91 >B3KAT4.1 B3KAT4_9BACI Apurinic endonuclease Apn1 EC=3.1.21.2 Length = 373 Score = 575 bits (1481), Expect = e-162, Method: Compositional matrix adjust. Identities = 272/364 (74%), Positives = 310/364 (85%) Query: 1 VSCADKGLLSAANEANEYGSTSFMIYTGAPQNTRRKPIESMYPAEGKQAMKDNGVEEIVV 60 VS +DKGLL+A EA YGS+SFMIYTGAPQNTRRKPI+SM+ EGK AMK++G+EEIVV Sbjct: 8 VSFSDKGLLTATAEATSYGSSSFMIYTGAPQNTRRKPIDSMFIEEGKAAMKESGIEEIVV 67 Query: 61 HAPYIINLGSYKDSTYQLAVDFLQEEIRRTHALEVKHIVLHPGAYTDKDAEYGVQRIADG 120 HAPYIINLGSYK +T++LAV FLQEEIRRTHA+ VK+IVLHPGAYTDKDAEYG+ RIA+G Sbjct: 68 HAPYIINLGSYKPNTFELAVSFLQEEIRRTHAVGVKNIVLHPGAYTDKDAEYGIDRIAEG 127 Query: 121 LNEVLGGTNETEVHIALETMAGKGTEVGRSFEEIASIIDKVVHNERLSICLDTCHIHDAG 180 LNEVLGGT+ETEV IALETMAGKGTE+GRSFEEIASIIDKV +N+RL+ICLDTCHIHDAG Sbjct: 128 LNEVLGGTDETEVKIALETMAGKGTEMGRSFEEIASIIDKVKYNDRLAICLDTCHIHDAG 187 Query: 181 YDIVGDLDGVLQQFDQIIGLSRLGVIHINDSKNPRGAGKDRHTPIGSGYIGFETINKVVH 240 YDIV DLDGVL+QFD++IGL RL V+H+NDSKNPRGAGKDRHTPIG+G+IG+E I +VVH Sbjct: 188 YDIVNDLDGVLEQFDKLIGLDRLTVVHVNDSKNPRGAGKDRHTPIGTGWIGYEAIQRVVH 247 Query: 241 HEALAGLPFILETPWIGKDAKKLRPMYEVEIALLRGTVAERFGAEFLNEVEELHAFFAKQ 300 HE L G PFILETPWIGKD K RPMYE+EIALLRG V RFG F +VEEL AFFAK+ Sbjct: 248 HEKLQGRPFILETPWIGKDPKTQRPMYEIEIALLRGDVEGRFGPAFTQQVEELGAFFAKE 307 Query: 301 EFDPRQYVLNVWELLKNDXXXXXXDPREPLERLYDTVAAAQLFPQLSEEAVNQRLIAWLA 360 + R +VL+ W LLKND DPREP+ERLYD V AA L LSEE +NQRLIAWL+ Sbjct: 308 GIESRSFVLDTWTLLKNDAKAKKADPREPMERLYDMVMAAGLLSDLSEEQINQRLIAWLS 367 Query: 361 GKQL 364 G L Sbjct: 368 GVSL 371 >B1DJI5.1 B1DJI5_9BACL Apurinic endonuclease Apn1 EC=3.1.21.2 Length = 376 Score = 543 bits (1398), Expect = e-152, Method: Compositional matrix adjust. Identities = 251/366 (68%), Positives = 303/366 (82%), Gaps = 1/366 (0%) Query: 1 VSCADKGLLSAANEANEYGSTSFMIYTGAPQNTRRKPIESMYPAEGKQAMKDNGVEEIVV 60 VS +DKGLL+AA EA YGS+SFMIYTGAPQNTRRKPIE++Y EGKQ M+ G+ EIVV Sbjct: 8 VSFSDKGLLTAAKEAVSYGSSSFMIYTGAPQNTRRKPIETLYIEEGKQMMEQAGINEIVV 67 Query: 61 HAPYIINLGSYKDSTYQLAVDFLQEEIRRTHALEVKHIVLHPGAYTDKDAEYGVQRIADG 120 HAPYIINLGSYK+ T++LAV FLQEEIRRT + V++IVLHPGAYTDKDAEYG+ RIA+G Sbjct: 68 HAPYIINLGSYKEDTFELAVRFLQEEIRRTDYIGVRNIVLHPGAYTDKDAEYGIARIAEG 127 Query: 121 LNEVLGGTNETEVHIALETMAGKGTEVGRSFEEIASIIDKVVHNERLSICLDTCHIHDAG 180 LNEVL GT +T V+IALETMAGKGTE+GRSFEE+A IIDKV +N+RL++C+DTCH+HDAG Sbjct: 128 LNEVLEGTKDTNVNIALETMAGKGTEIGRSFEELAQIIDKVQNNDRLTVCMDTCHMHDAG 187 Query: 181 YDIVGDLDGVLQQFDQIIGLSRLGVIHINDSKNPRGAGKDRHTPIGSGYIGFETINKVVH 240 YD++ DLDGVLQ+FDQ +GL R+ V+H+NDSKNP GAGKDRH P+G+G++G E I+++VH Sbjct: 188 YDLINDLDGVLQKFDQTVGLDRVAVVHVNDSKNPVGAGKDRHAPVGAGWLGHEAIHRIVH 247 Query: 241 HEALAGLPFILETPWIGKDAKKLRPMYEVEIALLRGTVAERFGAEFLNEVEELHAFFAKQ 300 HEALAG PFILETPWIGKD KK PMYE EIALLRG RFG F + VE LH F+ KQ Sbjct: 248 HEALAGRPFILETPWIGKDDKKQFPMYEAEIALLRGDAMGRFGESFFDHVERLHHFYGKQ 307 Query: 301 EFDPRQYVLNVWELLKNDXXXXXXDPREPLERLYDTVAAAQLFP-QLSEEAVNQRLIAWL 359 E DPRQ+VL+ W++LKND DPREP+ERLYD ++ + P L+EE +NQR+ AW Sbjct: 308 EIDPRQFVLSTWDVLKNDAKAKKADPREPMERLYDLLSESDALPGDLTEEQINQRITAWF 367 Query: 360 AGKQLL 365 AGKQLL Sbjct: 368 AGKQLL 373 >C0ZIS9.1 C0ZIS9_BREBN Endonuclease IV EC=3.1.21.2 Length = 372 Score = 516 bits (1328), Expect = e-144, Method: Compositional matrix adjust. Identities = 244/360 (67%), Positives = 288/360 (80%) Query: 1 VSCADKGLLSAANEANEYGSTSFMIYTGAPQNTRRKPIESMYPAEGKQAMKDNGVEEIVV 60 VS + KGLL+AA EA YGS++FMIYTGAPQNTRRKPIE Y EGK+ M GV+EIVV Sbjct: 8 VSFSGKGLLNAAQEAATYGSSTFMIYTGAPQNTRRKPIEDQYITEGKEVMAKQGVDEIVV 67 Query: 61 HAPYIINLGSYKDSTYQLAVDFLQEEIRRTHALEVKHIVLHPGAYTDKDAEYGVQRIADG 120 HAPYIINLGSYKD TY+LAV FLQEEIRRT + VK+IVLHPGAYTDKDAEYG+ RIA+G Sbjct: 68 HAPYIINLGSYKDDTYELAVRFLQEEIRRTDYIGVKNIVLHPGAYTDKDAEYGIARIAEG 127 Query: 121 LNEVLGGTNETEVHIALETMAGKGTEVGRSFEEIASIIDKVVHNERLSICLDTCHIHDAG 180 LNEVL G +T+V IALETMAGKGTE+GRSFEEIA+II+KV N RL++C+DTCHIHDAG Sbjct: 128 LNEVLTGIKDTDVKIALETMAGKGTEIGRSFEEIAAIIEKVEDNSRLTVCMDTCHIHDAG 187 Query: 181 YDIVGDLDGVLQQFDQIIGLSRLGVIHINDSKNPRGAGKDRHTPIGSGYIGFETINKVVH 240 YDIV D DGVL+QFD+ IGL RL V+H+NDSKN RGAGKDRH PIG+G IGF+ +N +V+ Sbjct: 188 YDIVNDFDGVLEQFDRTIGLDRLAVVHLNDSKNFRGAGKDRHAPIGAGLIGFDAMNYIVN 247 Query: 241 HEALAGLPFILETPWIGKDAKKLRPMYEVEIALLRGTVAERFGAEFLNEVEELHAFFAKQ 300 HE + LP +LETPWI K+ RPMYE EIALLRG V +RFG EF++ VE L FF KQ Sbjct: 248 HEKIRHLPLVLETPWISKEKGNERPMYEAEIALLRGEVDKRFGNEFMDHVERLDFFFRKQ 307 Query: 301 EFDPRQYVLNVWELLKNDXXXXXXDPREPLERLYDTVAAAQLFPQLSEEAVNQRLIAWLA 360 + R+YV+ +WELLKND D REP+ERLYD V A+LFP+L+EE +N RL A+ A Sbjct: 308 DVTRREYVVGIWELLKNDAKAKKADGREPMERLYDMVKEARLFPELTEEQINHRLTAYFA 367 >A8VUC2.1 A8VUC2_9BACI Deoxyribonuclease IV (Phage-T(4)-induced) EC=3.1.21.2 Length = 300 Score = 343 bits (881), Expect = 1e-92, Method: Compositional matrix adjust. Identities = 168/271 (61%), Positives = 207/271 (76%), Gaps = 3/271 (1%) Query: 6 KGLLSAANEANEYGSTSFMIYTGAPQNTRRKPIESMYPAEGKQAMKDNGVEEIVVHAPYI 65 K LL+A+ EA YGST+FMIYTGAPQNTRRK IE + G+ M +G++ IVVHAPYI Sbjct: 13 KMLLAASEEAAGYGSTTFMIYTGAPQNTRRKAIEDLNIEAGRAHMNQHGIDHIVVHAPYI 72 Query: 66 INLG-SYKDSTYQLAVDFLQEEIRRTHALEVKHIVLHPGAYTDKDAEYGVQRIADGLNEV 124 IN+ + K T+QL VDFL+ EI RT AL K IVLHPGA+ A+ G+ +I +GLNEV Sbjct: 73 INIANTTKPETFQLGVDFLKSEIDRTEALGAKQIVLHPGAHVGAGADQGIAKIIEGLNEV 132 Query: 125 LGGTNETEVHIALETMAGKGTEVGRSFEEIASIIDKVVHNERLSICLDTCHIHDAGYDIV 184 + + +V IALETMAGKG+E GRSFEE+A IID V HNE+LS+C DTCH+HDAGYDIV Sbjct: 133 I--DPDIDVQIALETMAGKGSECGRSFEELAKIIDGVTHNEKLSVCFDTCHVHDAGYDIV 190 Query: 185 GDLDGVLQQFDQIIGLSRLGVIHINDSKNPRGAGKDRHTPIGSGYIGFETINKVVHHEAL 244 DLDGVL++FD I+GL RL V+HINDSKN RGA KDRH IG GYIGFE I++++HHEA Sbjct: 191 NDLDGVLKEFDDIVGLDRLKVLHINDSKNERGASKDRHENIGFGYIGFEAIDRILHHEAF 250 Query: 245 AGLPFILETPWIGKDAKKLRPMYEVEIALLR 275 +P ILETP++G+D KK P Y+ EIA+LR Sbjct: 251 GDIPKILETPYVGEDKKKRVPPYKEEIAMLR 281 >C5D4R0.1 C5D4R0_9BACI Apurinic endonuclease Apn1 EC=3.1.21.2 Length = 298 Score = 340 bits (871), Expect = 2e-91, Method: Compositional matrix adjust. Identities = 170/286 (59%), Positives = 212/286 (74%), Gaps = 6/286 (2%) Query: 6 KGLLSAANEANEYGSTSFMIYTGAPQNTRRKPIESMYPAEGKQAMKDNGVEEIVVHAPYI 65 K LL+A+ EA YG+ +FMIYTGAPQNTRRK IE + G MK++G+EEIVVHAPYI Sbjct: 14 KMLLAASEEAVSYGANTFMIYTGAPQNTRRKKIEELNIEAGLAHMKEHGIEEIVVHAPYI 73 Query: 66 INLGSYKD-STYQLAVDFLQEEIRRTHALEVKHIVLHPGAYTDKDAEYGVQRIADGLNEV 124 IN+G+ K+ T+ L V+FL+ EI RT A+ K IVLHPGA+ E G+++I +GLNEV Sbjct: 74 INIGNTKNPDTFALGVEFLRSEIERTEAIGAKQIVLHPGAHVGAGPEAGIRKIIEGLNEV 133 Query: 125 LGGTNETEVHIALETMAGKGTEVGRSFEEIASIIDKVVHNERLSICLDTCHIHDAGYDIV 184 L T E V IALETMAGKG+E GRSFEE+A IID V HNE+LS+C DTCH HDAGY+IV Sbjct: 134 L--TREQTVQIALETMAGKGSECGRSFEELAQIIDGVTHNEKLSVCFDTCHTHDAGYNIV 191 Query: 185 GDLDGVLQQFDQIIGLSRLGVIHINDSKNPRGAGKDRHTPIGSGYIGFETINKVVHHEAL 244 D DGVL++FD+IIGL RL V+HINDSKNPRG+ KDRH IG G+IGF +N +VHH L Sbjct: 192 DDFDGVLEEFDRIIGLDRLKVLHINDSKNPRGSRKDRHENIGFGHIGFAALNYIVHHPQL 251 Query: 245 AGLPFILETPWIGKDAKKLRPMYEVEIALLRGTVAERFGAEFLNEV 290 A +P ILETP++G+D +P Y+ EIA+LR V F E L ++ Sbjct: 252 ADIPKILETPYVGEDKNNKKPPYKHEIAMLRAQV---FDEELLTKI 294 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.318 0.137 0.398 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,229,215,929 Number of extensions: 97544689 Number of successful extensions: 211196 Number of sequences better than 1.0e-05: 834 Number of HSP's gapped: 209523 Number of HSP's successfully gapped: 834 Length of query: 368 Length of database: 3,060,696,203 Length adjustment: 137 Effective length of query: 231 Effective length of database: 1,770,017,148 Effective search space: 408873961188 Effective search space used: 408873961188 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)